Phialemonium Guarroi Fungal Planet Description Sheets 451

450 Persoonia – Volume 42, 2019

Phialemonium guarroi Fungal Planet description sheets 451

Fungal Planet 941 – 19 July 2019 Phialemonium guarroi Rodr.-Andr., Cano & Stchigel, sp. nov.

Etymology. In honour of the mycologist Josep Guarro Artigas. Notes — Phialemonium guarroi was recovered from a soil sample collected in Punta Gorda, La Palma, Canary Islands, Classiﬁcation — Cephalothecaceae, Sordariales, Sordari Spain. The genus Phialemonium was established by Gams & omycetes. McGinnis (1983). Phialemonium contains seven accepted spe- Mycelium composed of septate, hyaline, smooth- and thin- cies, mostly isolated from environmental sources and human walled hyphae, 1.5–2 μm wide, becoming cinnamon and specimens (Rivero et al. 2009, Perdomo et al. 2011, Guarro moniliform in old cultures, whose cells reach up to 10 μm diam. 2012, Crous et al. 2015b). Phialemonium guarroi is morpho- Conidiophores absent or poorly differentiated, often consisting logically similar to Phialemonium inflatum. However, the new in single lateral phialides and adelophialides borne directly species can be distinguished from the latter due to the pro- from aerial hyphae, occasionally composed of a short stipe duction of phialides which proliferate percurrently to form long of up to 15 μm long and bearing 1–3 phialides in an irregular chains (feature not reported in P. inflatum) and the production of arrangement. Phialides abundant, hyaline, smooth-walled, smaller conidia than those of P. inflatum. Based on a megablast flask-shaped, with more or less inflated at the base and tapering search of NCBIs GenBank nucleotide database, the closest hit towards the top, 12–15 × 1.5–2 μm, percurrently proliferating using the ITS sequence is the ex-type strain of P. inflatum CBS to form long chains in old cultures. Adelophialides hyaline, 259.39 (GenBank LT633912; Identities = 490/535 (92 %), 10 smooth-walled, cylindrical but slightly tapering towards the top, gaps (1 %)); using the LSU sequence was the same ex-type 12–15 × 1.5–2 μm. Conidia hyaline, aseptate, lemon-shaped, strain of P. inflatum (GenBank LT633912; Identities = 845/857 3–3.5 × 1.5–2 μm, smooth-walled, produced in chains of up (99 %), no gaps). The ITS-LSU phylogenetic tree corroborated to 25 conidia, with a cylindrical-truncate scar at both ends. the placement of our isolate as a new species of Phialemonium, Chlamydospores and sexual morph not observed. being located phylogenetically close to P. inflatum. Culture characteristics — Colonies on OA reaching 9–10 mm diam after 2 wk at 25 °C, flattened, velvety, grey (6B1; Korne rup & Wanscher 1978), margins regular, sporulation sparse, Phialemonium sp. FMR 17080 RAxML/BI ITS-LSU exudate absent; reverse pale yellow (3A3), diffusible pigment absent. Colonies on PCA attaining 10–11 mm diam after 2 wk at 25 °C, flattened, velvety, white (4A2), margins regular, spo rulation abundant, exudate absent; reverse yellowish grey Phialemonium obovatum CBS 279.76 (3B2), diffusible pigment absent. Colonies on PDA of 12–13 mm Phialemonium obovatum CBS 730.97 T diam after 2 wk at 25 °C, elevated, velvety to floccose, margin irregular, yellowish brown (5E4) at centre and yellowish grey Cephalotheca foveolata UTHSC 08-2766 100 / 0.99 (3B2) at edge, exudate absent, sporulation abundant; reverse Cephalotheca foveolata NBRC 100905 T olive brown (4E6) at centre and white (4A1) at edge, diffusible Phialemonium atrogriseum CBS 604.67 T pigments absent. Minimum, optimal and maximum temperature of growth (on PDA): 15 °C, 25 °C and 30 °C, respectively. Phialemonium atrogriseum CBS 306.85

Typus. Spain, Canarias, Santa Cruz de Tenerife province, La Palma, Punta Phialemonium guarroi FMR 17080 T Gorda, isolated from soil, Aug. 2009, A.M. Stchigel & M. Calduch (holotype T CBS H-23924, cultures ex-type FMR 17080 = CBS 145626; ITS and LSU 91 / 0.99 Phialemonium globosum CBS 131713 sequences GenBank LR535737 and LR535738, MycoBank MB830182). T 78 / 0.99 Phialemonium limoniforme FMR 13627

94 / - Phialemonium inflatum NBRC 31965

Phialemonium inflatum CBS 259.39 T 93 / 0.98 Cephalotheca sulfurea CBS 135.34

Phialemonium dimorphosporum CBS 491.82

Phialemonium curvatum UTHSC 06-4324

Lecythophora luteoviridis CBS 206.38 T Lecythophora lignicola CBS 267.33 T

0.04

Maximum likelihood tree obtained from the ITS-LSU alignment of our isolate and sequences retrieved from GenBank. The tree was built by using RAxML CIPRES (http://www.phylo.org/sub_sections/portal/) and the analysis of pro bability was run in MrBayes v. 3.2.6 (Ronquist et al. 2012). Bootstrap support (BS) values ≥ 70 % and Bayesian posterior probability (PP) values ≥ 0.95 are presented at the nodes. Fully supported branches (100 % BS / 1 PP) are indicated in bold. Lecythophora luteoviridis CBS 206.38 and Lecythophora lignicola CBS 267.33 were used as outgroup. The new species proposed in Colour illustrations. Typical vegetation of La Palma island, Canary Islands this study is indicated in bold. TRepresents the ex-type strains of the taxa archipelago, Spain (Photo credit: A. DeCort). Moniliform cells, adelophialides, employed in this analysis. phialides and conidia. Scale bars = 10 µm.

Ernesto Rodríguez-Andrade, José F. Cano-Lira & Alberto M. Stchigel, Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV), Sant Llorenç 21, 43201 Reus, Tarragona, Spain; e-mail: [email protected], [email protected] & [email protected]