(Ex Vivo) with Newly Diagnosed Acute Myeloid
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PHARMACOLOGICAL PROFILE OF CYTARABINE AND IDARUBICIN IN PATIENT SAMPLES (EX VIVO) WITH NEWLY DIAGNOSED ACUTE MYELOID LEUKEMIA IDENTIFIES RESPONDERS VS NON RESPONDERS Pau Montesinos1, David Martinez1, Joaquín Martínez-López2, Raimundo Garcia3, Jaime Pérez de Oteyza4, Pascual Fernandez5, Josefina Serrano6, Ángeles Fernández7, Pilar Herrera8, Arancha Alonso9, Ataulfo Gonzalez10, Concepción Bethancourt11, Esperanza Lavilla12, Juan Antonio Vera13, Begoña Navas14, Gabriela Rodríguez-Macías15, Juan Antonio López16, Santiago Jiménez17, Adriana Simiele18, Bernardo González19, Jose Angel Hernández20, Raúl Córdoba21, Consolación Rayón22, Carmen Burgaleta23, Jordi Sierra24, Iñaki Trocóniz25, Ignacio Ortega26, Andrew G Bosanquet27, Daniel Primo28, Pilar Hernández-Campo28, Julian Gorrochategui28, Jose L. Rojas28, Teresa A. Bennett28 , Belén Liébana28, Rocío López28, Joan Ballesteros Nobell28 , Federico Moscardó1, Miguel Ángel Sanz1 1Haematology, Hospital Universitari i politecnic La Fe, Valencia, Spain, 2Haematology, Hospital Universitario Doce de Octubre, Madrid, Spain,3Haematology, Hospital Universitario General de Castellón, Castellón, Spain, 4Haematology, Hospital de Madrid Norte Sanchinarro, Madrid, Spain, 5Haematology, Hospital General Universitario de Alicante, Alicante, Spain, 6Haematology, Hospital Universitario Reina Sofía, Córdoba, Spain, 7Haematology, Complejo Hospitalario Xeral Cíes de Vigo, Vigo, Spain, 8Haematology, Hospital Universitario Ramón y Cajal, Madrid, Spain, 9Haematology, Hospital Quirón Madrid, Spain, 10Haematology, Hospital Clínico San Carlos, Madrid, Spain, 11Hospital Regional Universitario Carlos Haya, Málaga, Spain, 12Hospital Universitario Lucus Augusti, Lugo, Spain, 13Hospital Universitario Virgen de la Macarena, Sevilla, Spain, 14Hospital Moncloa, Madrid, Spain, 15Haematology, Hospital Universitario Gregorio Marañón, Madrid, Spain, 16Haematology, Complejo Hospitalario de Jaén, Jaén, Spain, 17Haematology, Hospital Universitario de Gran Canaria Doctor Negrín, Gran Canaria, Spain, 18Haematology, Hospital Povisa, Vigo, Spain, 19Haematology, Hospital Universitario de Canarias, Tenerife, Spain, 20Haematology, Hospital Universitario Infanta Leonor, Madrid, Spain, 21Haematology, Hospital Universitario Infanta Sofía, Madrid, Spain, 22Haematology, Hospital Universitario Central de Asturias, Oviedo, Spain, 23Haematology, Hospital Universitario Príncipe de Asturias, Madrid, Spain, 24Haematology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain, 25Universidad de Navarra, Pamplona, Spain, 26Universidad del País Vasco, Bilbao, Spain, 27Bath Cancer Research, Bath, England, 28Vivia Biotech, Madrid, Spain, ABSTRACT METHODS METHODS Background and objectives: Complete remission (CR) after induction therapy is the first treatment goal in acute myeloid leukemia (AML) patients. The aim of ExviTech© Platform this study is to determine the ability of the Vivia’s novel ex vivo drug sensitivity platform Exvitech analyzing leukemic cell death to predict the CR rates after Robotic Sample Automated FCM BeckmanCoulter Cyan Flow Proprietary Activity Base Bioinformatics Results induction chemotherapy with cytarabine (Ara-C) and idarubicin (Ida) in 1st line AML. Preparation Based Screening Cytometer Analysis Software + Clinical Info Eight different concentrations of each Whole sample vs. Isolated Leukocytes: A. A Patients and Methods: This non-interventional and prospective study included samples from patients over 18 years of age diagnosed with de novo AML in drug or drug combination is run for Dose-response curves for IDA and CYT in the used treatment protocols. The Spanish centers from the PETHEMA group. Marrow samples were collected at diagnosis, sent to the Vivia laboratories, and incubated for 48 hours in whole max concentration used is listed isolated leukocytes and whole sample. samples in well plates containing Ara-C, Ida, or the combination Ara-C + Ida, each at 8 different concentrations to calculate dose responses. Annexin V-FITC Data, from sample 6 below, displays a log was used to quantify the drug-induced apoptosis. Pharmacological responses are calculated using pharmacokinetic population models. Induction response 1 2 3 4 5 6 7 8 9 10 11 12 difference in the EC50s for IDA, but equal A DMSO DMSO 0.47% was assessed according to the Cheson criteria (2003). Patients attaining a CR/CRi were classified as responders. The remaining patients were considered as DMSO 0.47% results for CYT. B. The EC50 (y-axis) of the B CYTARABINE 150µM C whole sample and the isolated leukocyte resistant. Patients dying during induction response assessment were non-evaluable. The correlation between the pharmacologic parameters and the clinical IDARUBICIN 1.5µM D response was modeled using a generalized additive model with a logit link and a binomial distribution for residuals. Kernel density estimates were then used FLUDARABINE 127.5µM fraction from 9 patient samples with E MITOXANTRONE to plot empirical probability density functions of the model fitted values in the response scale for both groups. Their separation was quantified as the area 10µM cytarabine. C. EC50 of the same samples to F CLOFARABINE 74.8µM B under the ROC curve and a cut point was selected using the Youden’s criterion to optimize the classification probabilities (sensitivity, specificity). 95% G idarubicin. PANOBINOSTAT 30µM H confidence intervals for sampling errors were calculated for all these quantifiers. Screening Setup and Workflow Data Analysis: performed using the Results: 199 patient samples were used to calculate the dose response curves for Ara-C alone, Ida alone, and synergism of the Ara-C plus Ida combination. DAY 1 1 2 3 4 5 6 7 8 9 10 11 12 DAY 3 PB or BM A population approach using NONMEM 7.2.: For clinical correlation we used 100 patients with a median age of 52 years (range 26 to 85). Dose responses for Ara-C alone are shown in Figure 3.A; note that DMSO DMSO 0.47% Analysis and Import B DMSO 0.47% DAUNORUBICIN 3µM population PD modelling of the ex vivo for many samples there is a significant number (>XX%) of resistant cells to Ara-C (bracket). This is a strong clinical predictor of resistance because in the into ActivityBase C ETOPOSIDE 225µM Split sample response vs concentration data in REPORT GENERATED D 100µM patient the drug will never be present at these high doses for 48h. The second variable that is a good predictor of response is the synergism between these 2 6-THIOGUANINE E monotherapy (fig.1), establishing for each drugs. The generalized additive model identified an algebraic combination of these variables that included also the maximum percentage of cells depleted by CYCLOPHOSPHAMIDE100µM C F 15µM MEPHALAN patient the 95% prediction intervals (PI) of Ara-C that yielded the best marker to separate both groups of patients. The overlap between the probability density functions of the fitted values was small G 5 -AZACYTIDINE 50µM the isobologram from each individual H (figure 5). The area under the corresponding ROC curve was 0.853 (0.773, 0.933) and the classification probabilities for the optimal cut point, expressed as Sample Validation/ Cell parameter (fig.4) computation of the percentages, were 81% (64% to 91%) and 80% (69% to 88%) for sensitivity and specificity, respectively. Results are shown in Figure 6; sixty-nine patients (69%) Count Apoptotic achieved CR after Ida + Ara-C, and the remaining 31 (31%) were resistant. When the ex vivo test predicted a patient as sensitive it was correct in 55/61 cases combination index using raw data Plated Analysis with: descriptors from combination experiments. (90%) and when it predicted resistant it was correct 25/39 cases (64%). Overall, the clinical response of 80 patients (80%) was correctly anticipated. Drugs Annexin V Anti-CD45 Drug-Sample Plates Annexin V Anti-CD14 Conclusions: This study shows that this novel ex vivo pharmacological profile test is able to predict the clinical response to Ida + Ara-C induction. Further Chou and Talalay. 2010. Cancer Research 48H Anti-CD34 Anti-CD64 Live efforts are in progress to refine the prediction model to remove as much as random variability as possible and to identify other sources of variability. A PM Anti-CD117 Anti-CD13 70: 440-446. Anti-HLA-DR Anti-CD11b test-adapted Clinical Trial is planned to evaluate the impact of the PM test over clinical outcomes. RESULTS Figure 6 Figure 1 Objectives & Study Design Figure 2 Pharmacological ex vivo Data: Figure 3 Individual Dose Response Curves Figure 5 Logistic additive model of ex vivo CYT-IDA vs Clinical Outcome 90% Prediction ex vivo Personalized Medicine Test Single drugs & Synergism A cells Resistant ROC Curve A Empirical probability distributions of the B Clinical outcome Background & Objectives Statist. signif. low conf. limit AUC 0.872 > 0.5 marker in resistant vs. sensitive patients Sensitivity: 87%, specificity: 91% RESISTANT SENSITIVE Subtotal • Complete remission (CR) after induction is the A at optimal cutpoint (star, Youden’s criterion) Positive predictive value % first treatment goal in AML patients 5 20 5 Complete remission 32.5% SURV_IDX (%) SURV_IDX 25 Partial remission or resistant disease 26.0% RESISTANT 6.5% 80.00 • Response to chemotherapy is the main 4 prognostic factor Negative predictive 3 3 49 value % • There is no test accurately predicting the B 52 67.5% Exvivo responseExvivo 63.6% SENSITIVE Density 3.9% 94.23 response to specific drug schedules. 2 [CYTARABINE] (µM) • The aim is to determine the ability of an ex- 1 Sensitivity % Specificity % Prediction rate % vivo drug sensitivity