Monitoring Population Size of Endangered Hawaiian Duck and Prevalence of Mallard/Hawaiian Duck Hybrids on Hanalei and Hulē‘Ia Nwrs Kaua‘I, Hawai‘I Final Report
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Monitoring population size of endangered Hawaiian duck and prevalence of Mallard/Hawaiian duck hybrids on Hanalei and Hulē‘ia NWRs Kaua‘i, Hawai‘i Final Report Koloa maoli female (Photo M. Couffer/USFWS) Submitted 1 September 2018: Inventory and Monitoring Program Initiative, Region 1, USFWS Submitted by: Dr. Bruce D. Dugger Christopher P. Malachowski Department of Fisheries and Wildlife Department of Fisheries and Wildlife Oregon State University Oregon State University 104 Nash Hall 104 Nash Hall Corvallis, OR 97331 Corvallis, OR 97331 Phone: 541-737-2465 Phone: 215-850-1978 E-mail: [email protected] E-mail: [email protected] Kimberly J. Uyehara Kaua‘i National Wildlife Refuge Complex U.S. Fish and Wildlife Service PO Box 1128 Kīlauea, HI 96754 Phone: 808-635-9309 E-mail: [email protected] ‘‘The findings and conclusions in this article are those of the author(s) and do not necessarily represent the views of the U.S. Fish and Wildlife Service.’’ TABLE OF CONTENTS 1. EXECUTIVE SUMMARY………………………………………... 1 2. INTRODUCTION………………………………………………….. 3 a. Objectives………………………………………………….. 4 3. STUDY AREA……………………………………………………… 5 4. METHODS………………………………………………………….. 5 5. RESULTS…………………………………………………………… 14 6. DISCUSSION……………………………………………………….. 19 7. ACKNOWLEDGMENTS…………………………………………… 26 8. LITERATURE CITED………………………………………………. 27 * All photos credited C. Malachowski unless otherwise noted ii EXECUTIVE SUMMARY The Hawaiian duck (hereafter Koloa) was listed as an endangered species in 1967. Key threats to species recovery are the lack of basic demographic information, like population size that can be used to inform recovery decisions, and genetic introgression with feral Mallards. Here we report on the results of a joint research project and conservation action to achieve the following objectives: 1) investigate the value of using banded birds and a mark-resight analysis approach to estimate the population size of Koloa using Hanalei and Hulē‘ia NWRs; 2) Determine the prevalence of Mallard/Koloa hybrids on Hanalei and Hulē‘ia NWRs; 3) Collect data to help refine the Mallard/Koloa hybrid key to improve implementation of hybrid removal activities at both Kaua‘i refuges and James Campbell NWR on O‘ahu; 4) Conduct field surveys at Hanalei and Hulē‘ia to survey for extent and seasonality of intra-island movements; and 5) Develop operational survey to annually detect and remove hybrids from Hanalei and Hulē‘ia NWRs; explore application of these methods to James Campbell NWR on O‘ahu. Between December 2010 and December 2012 we captured and banded 835 unique Koloa, most at Hanalei NWR (97.0%); 78.4% of captured birds were male. The male-bias in the sex ratio was higher than previously reported for any continental dabbling duck, was consistent with other measures of the sex ratio at Hanalei NWR, and is something that should be investigated. We detected no band loss or excessive wear looking at 918 recaptures through November 2015. Persistent banding increased the average number of banded birds detected on surveys from 6.2 in 2011 to 34.5 in 2013. Of those birds that were determined to be banded, we were able to read the alpha numeric code for just over 39% of bands in 2011 and 52% in 2013; however, for most birds it was difficult to read bands during visual surveys. Nine captured males (1.1% of our capture sample) scored as hybrids using a plumage and morphometric key and were subsequently humanely euthanized. Subsequent genetic testing assigned 8 of those 9 birds a probability of 95% being Koloa. We convincingly demonstrated that a large number of Koloa can be safely captured using a traditional trapping technique developed for waterfowl, the baited swim-in trap. However, the considerable survey effort needed to detect and read alpha-numeric color bands in the field indicated that a band-resighting will not work for the surveyor who needs to efficiently cover many sites in a single day or for busy refuge biologists who do not have the time available for band searching with considerable intensity. Thus, we recommend future efforts to estimate population size or survival using banded birds adopt a capture-recapture methodology. The 1 occurrence of Mallard-Koloa hybrids at Hanalei NWR is very low, a conclusion supported by subsequent genetic testing of a large sample of our captured birds. Previous estimates for the prevalence of hybrids on the island have been based on observations of “hybrid-looking” birds seen during periodic surveys. Our work on the plumages and molts in conjunction with collaborators at the University of California Davis has revealed significantly more variation in male plumages than previously described for Koloa. This variation is known to occur in birds that are genetically confirmed to be Koloa; thus, any estimate of hybrid prevalence based solely on visual sightings will likely be biased high for males. Continued application of the existing plumage/morphometric key would result in false positives for 1% of birds (i.e., they score as hybrids when genetically they score as Koloa). Given examples of variation in culmen measurements among measurers and among attempts for a single measurer on a single bird, a more risk adverse method (e.g., minimize false positives) of using the morphometric key would involve building in measurement error to the decision making threshold criteria values. Despite banding more than 1,000 Koloa on Kaua‘i since 2010, and extensive survey activities on Oʻahu during summer 2015, no birds banded on Kaua‘i have been observed on O‘ahu. We conclude the report by describing a protocol that could be used to implement a capture-recapture program at Hanalei NWR to estimate Koloa population size and possibly survival. 2 INTRODUCTION The Hawaiian duck or Koloa maoli (Anas wyvilliana, hereafter Koloa) is endemic to Hawai‘i and one of three extant waterfowl species occurring on the Hawaiian Islands (Olson and James 1982, Engilis et al. 2002). Relatively common during the early 1900s, the Koloa has since experienced a significant statewide population decline because of factors that include wetland loss, over harvest by hunters, and introduced mammalian predators. More recently, the genetic integrity of the species has been threatened by hybridization with feral Mallards (Engilis et al. 2002). Consequently, Koloa was listed as a Federally-endangered species in 1967 and has the highest recovery priority for the four listed wetland birds that occur on the main Hawaiian Islands (USFWS 2011). Kaua‘i is the only island that likely supports a population of non-hybridized Koloa (Wells et al. 2016), and Hanalei and Hulē‘ia National Wildlife Refuges (NWRs) are the number one and two most important sites for Koloa in the state. There is currently no credible estimate of Koloa population size at any spatial scale (Engilis et al. 2002). Although state biannual counts may provide an index for wetlands, counts do not include streams where many Koloa are believed to reside on Kaua‘i (Schwartz and Schwartz 1953, Swedberg 1967). The state biannual count peaked at 524 for the all-island survey and 459 for Kaua‘i in January 2004 (Hawai‘i DLNR unpublished data 1986-2006). However, during banding activities from December 2010 to March 2011 on Hanalei NWR we banded 291 individual Koloa and resighting activities indicated that greater than 75% of birds on the refuge were not banded (C. Malachowski unpubl. data), which indicated the count data were likely biased low. Additionally, limited radio telemetry data from Hanalei NWR (C. Malachowski unpubl. data) indicate there are common movements of Koloa between Hanalei NWR and surrounding areas indicating that mid-day counts on the refuge likely are not adequately characterizing the role that Hanalei NWR plays in meeting the daily habitat needs for Koloa in the Hanalei region. These facts point to a need to investigate other methods for estimating Koloa population size. Beginning in December of 2010, we initiated a joint conservation action and research project to begin identifying and removing hybrids from Hanalei and Hulē‘ia and investigate basic aspects 3 of Koloa ecology that could aid in species recovery including movements, survival, nest success and habitat use. Our initial success with trapping and banding Koloa (mentioned above) suggested that capture-recapture or mark-resighting methodologies might provide a means of estimating annual survival and regional population size as well as helping understand movements of Koloa around Kaua‘i and between islands. However, capturing and marking a sample of birds is only one step to make such calculations possible. Additional fieldwork to determine band retention rate, encounter rate with banded birds, and determine our ability to read bands in the field was also needed. Using funds from USFWS (Region 1 Refuges, Migratory Birds and Inventory and Monitoring and Oregon State University and in-kind contributions from the Kaua‘i NWRC) we continued our conservation effort to identify and remove hybrids and expanded our effort to survey for banded birds on Kaua‘i. Our objectives would help meet multiple monitoring and research objectives listed in the draft Hanalei and Hulē‘ia NWR Comprehensive Conservation Plan (CCP) and Hawaiian Waterbird Recovery Plan. The protocol for our survey also allowed us to move forward with key conservation and research activities that supported implementation and assessment of refuge management plans and inform refuge staff about the value of habitat management programs on both refuges. Objectives: 1. Investigate the value of using banded birds and a mark-resight analysis approach to estimate the population size of Koloa using Hanalei and Hulē‘ia NWRs. 2. Determine the prevalence of Mallard/Koloa hybrids on Hanalei and Hulē‘ia NWRs. 3. Collect data to help refine the Mallard/Koloa hybrid key to improve implementation of hybrid removal activities at both Kaua‘i refuges and James Campbell NWR on O‘ahu. 4. Conduct field surveys at Hanalei and Hulē‘ia to survey for extent and seasonality of intra- island movements.