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Laboratory Safety Manual
LABORATORY SAFETY MANUAL Environment, Health & Safety 1120 Estes Drive Extension CB# 1650 TABLE OF CONTENTS Section or Chapter Page Introduction i Emergency Telephone Numbers ii EHS – Scope of Service iii Condensed Laboratory Safety Information for New Research Personnel v Chapter 1 – Laboratory Safety at the University of North Carolina at Chapel Hill 1-1 Chapter 2 – Laboratory Safety Plan 2-1 Chapter 3 – General Safety Principles and Practices 3-1 Chapter 4 – Proper Storage of Chemicals in Laboratories 4-1 Chapter 5 – Protective Clothing and Equipment 5-1 Chapter 6 – Safe Handling of Chemicals 6-1 Chapter 7 – Highly Toxic Chemicals and Select Carcinogens 7-1 Chapter 8 – Reproductive Health 8-1 Chapter 9 – Controlled Substances 9-1 Chapter 10 – Fire Safety 10-1 Chapter 11 – Explosive and Reactive Chemical Hazards 11-1 Chapter 12 – Management of Laboratory Wastes 12-1 Chapter 13 – Safe Handling of Peroxidizable Compounds 13-1 Chapter 14 – Safe Handling of Laboratory Animals 14-1 Chapter 15 – Safe Handling of Biological Hazards 15-1 Chapter 16 – Biological Safety Cabinets 16-1 Chapter 17 – Laboratory Hoods 17-1 Chapter 18 – Safe Use of Nanomaterials 18-1 Revisions to Laboratory Safety Manual REV-1 Laboratory Safety Manual – the University of North Carolina at Chapel Hill INTRODUCTION This manual is a safety reference document for laboratory personnel at the University of North Carolina at Chapel Hill. The University’s Department of Environment, Health & Safety prepared this manual, followed by review and approval from both the University’s Laboratory and Chemical Safety Committee (LCSC) and the University Safety and Security Committee (USSC). -
Food, Food Chemistry and Gustatory Sense
Food, Food Chemistry and Gustatory Sense COOH N H María González Esguevillas MacMillan Group Meeting May 12th, 2020 Food and Food Chemistry Introduction Concepts Food any nourishing substance eaten or drunk to sustain life, provide energy and promote growth any substance containing nutrients that can be ingested by a living organism and metabolized into energy and body tissue country social act culture age pleasure election It is fundamental for our life Food and Food Chemistry Introduction Concepts Food any nourishing substance eaten or drunk to sustain life, provide energy and promote growth any substance containing nutrients that can be ingested by a living organism and metabolized into energy and body tissue OH O HO O O HO OH O O O limonin OH O O orange taste HO O O O 5-caffeoylquinic acid Coffee taste OH H iPr N O Me O O 2-decanal Capsaicinoids Coriander taste Chilli burning sensation Astray, G. EJEAFChe. 2007, 6, 1742-1763 Food and Food Chemistry Introduction Concepts Food Chemistry the study of chemical processes and interactions of all biological and non-biological components of foods biological substances areas food processing techniques de Man, J. M. Principles of Food Chemistry 1999, Springer Science Fennema, O. R. Food Chemistry. 1985, 2nd edition New York: Marcel Dekker, Inc Food and Food Chemistry Introduction Concepts Food Chemistry the study of chemical processes and interactions of all biological and non-biological components of foods biological substances areas food processing techniques carbo- hydrates water minerals lipids flavors vitamins protein food enzymes colors additive Fennema, O. R. Food Chemistry. -
2020 Program Book
PROGRAM BOOK Note that TAGC was cancelled and held online with a different schedule and program. This document serves as a record of the original program designed for the in-person meeting. April 22–26, 2020 Gaylord National Resort & Convention Center Metro Washington, DC TABLE OF CONTENTS About the GSA ........................................................................................................................................................ 3 Conference Organizers ...........................................................................................................................................4 General Information ...............................................................................................................................................7 Mobile App ....................................................................................................................................................7 Registration, Badges, and Pre-ordered T-shirts .............................................................................................7 Oral Presenters: Speaker Ready Room - Camellia 4.......................................................................................7 Poster Sessions and Exhibits - Prince George’s Exhibition Hall ......................................................................7 GSA Central - Booth 520 ................................................................................................................................8 Internet Access ..............................................................................................................................................8 -
Association of Three Biomarkers of Nicotine As Pharmacogenomic Indices of Cigarette Consumption in Military Populations DISSERTA
Association of Three Biomarkers of Nicotine as Pharmacogenomic Indices of Cigarette Consumption in Military Populations DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By William Arthur Matcham Graduate Program in Nursing The Ohio State University 2014 Dissertation Committee: Professor Karen L. Ahijevych, PhD, Advisor Professor Donna L. McCarthy, PhD Professor Kristine Browning, PhD Professor Yvette Conley, PhD Copyright by William Arthur Matcham 2014 ABSTRACT Tobacco-related diseases have reached epidemic proportions. There is no risk-free level of tobacco exposure. In the United States, tobacco use is the single largest preventable cause of death and disease in both men and women. Cigarette smoking alone accounts for approximately 443,000 deaths per year (one fifth of total US deaths) costing a staggering $193 billion per year in avoidable healthcare expenses and lost productivity. Literature shows military populations have rates of tobacco use two to three times higher than the civilian population. Military personnel returning from deployment in conflict areas can exceed 50% smoking prevalence. Research shows that genetic factors account for 40-70% of variation in smoking initiation and 50-60% of variance in cessation success. In the U.S., tobacco is responsible for more deaths than alcohol, AIDS, car accidents, illegal drugs, murders and suicides combined. This descriptive, cross-sectional study examined three of the biological markers used in tobacco research: the α4β2 brain nicotinic receptors (nAChR) that contribute to genetic risk for nicotine dependence, nicotine metabolite ratio (NMR) as a phenotypic marker for CYP2A6 activity, and bitter taste phenotype (BTP) to determine their impact on cigarette consumption in military populations. -
Molecular Clock Or Erratic Evolution?
Proc. Natl. Acad. Sci. USA Vol. 93, pp. 11729-11734, October 1996 Evolution Molecular clock or erratic evolution? A tale of two genes (neutral theory of evolution/glycerol-3-phosphate dehydrogenase/superoxide dismutase/Drosophila/Ceratitis) FRANcIsco J. AyALA*t, ELADIO BARRIO*t, AND JAN KWIATOWSKI* *Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697; and iDepartment of Genetics, University of Valencia, 46100-Burjassot, Valencia, Spain Contributed by Francisco J. Ayala, July 19, 1996 ABSTRACT We have investigated the evolution of glycer- Dorsilopha s.g. ol-3-phosphate dehydrogenase (Gpdh). The rate of amino acid Hirtodrosophila s.g. replacements is 1 x 10'10/site/year when Drosophila species Drosophila s.g. are compared. The rate is 2.7 times greater when Drosophila and Chymomyza species are compared; and about 5 times Zaprionus s.g. greater when any of those species are compared with the Sophophora s.g. medfly Ceratitis capitata. This rate of 5 x 10'10/site/year is also the rate observed in comparisons between mammals, or Chymomyz a between different animal phyla, or between the three multi- cellular kingdoms. We have also studied the evolution of Scaptodrosophila Cu,Zn superoxide dismutase (Sod). The rate of amino acid replacements is about 17 x 10'10/site/year when compari- Ceratitis sons are made between dipterans or between mammals, but only 5 x 10-10 when animal phyla are compared, and only 3 x 100 0 10-10 when the multicellular kingdoms are compared. The apparent decrease by about a factor of 5 in the rate of SOD evolution as the divergence between species increases can be My consistent with the molecular clock hypothesis by assuming the FIG. -
Taste Sensibility to Phenylthiocarbamide and Its Relationship to Food Preferences
127 ORIGINAL ARTICLE Taste sensibility to phenylthiocarbamide and its relationship to food preferences Sensibilidade à feniltiocarbamida e sua relação com a preferência alimentar Marcela Maria Pandolfi1. Charles Yea Zen Chow2. Luciana Sayumi Fugimoto Higashi2. Ana Thamilla Fonseca2. Myllena de Andrade Cunha2. Carolina Nunes França1,3. Patrícia Colombo-Souza1. 1 Post Graduation Program on Health Sciences, Santo Amaro University (UNISA). 2 Medicine Students, Santo Amaro University (UNISA). 3 Cardiology Division, Federal University of São Paulo (UNIFESP). ABSTRACT Introduction: Foods like cabbage, broccoli, pepper and wine, containing proteins such as phenylthiocarbamide (PTC), cause a bitter taste in some people. Studies showed the relation between tasting profile and the development of obesity, and consequently leading to cardiovascular disease. Objective: Compare the differences in the taste sensibility to PTC present in some foods in individuals classified according to the nutritional status. Methods: One hundred fifty-three patients classified as eutrophic, overweight or obese received one drop of each PTC solution in the tongue, since the most diluted to the individual perception to bitter taste, in a total of 15 increasing grades. Results: Participants were predominantly middle-aged females, eutrophics and supertasters. It was found a correlation between PTC solution and red wine in the three groups evaluated (eutrophic, obese and overweight). Besides, eutrophic and obese that disliked wine had more sensibility to PTC. Conclusion: Our main finding showed that eutrophic and obese that disliked red wine had more sensibility to PTC. We did not find differences in the sensitivity to PTC for the other foods analyzed (fried foods, fruit and vegetables). Keywords: Taste sensibility. -
Sexual Selection Operating in a Wild Population of Drosophila Robusta
BRIEF COMMUNICATIONS Evuluriun, 50(5), 1996, pp. 2095-2100 SEXUAL SELECTION OPERATING IN A WILD POPULATION OF DROSOPHILA ROBUSTA WILLIAMJ. ETGES Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas 72701 E-mail: [email protected] Key words.-Breeding site ecology, Drosophila, inversion polymorphism, life cycle components, mating success, natural selection, sexual selection. Received July 18, 1995. Accepted January 9, 1996. Almost 60 years have elapsed since the discovery of vari- (e.g., Prout 1971; Bundgaard and Christiansen 1972). Inver- ation in the polytene chromosomes of Drosophila (Sturtevant sion polymorphisms in cactophilic D. buzzatii have been stud- and Dobzhansky 1936). Inversion polymorphism studies have ied this way, a species that feeds and breeds in the fermenting revealed some of the intricacies of evolutionary change, but tissues of several species of prickly pear cactus. In general, even now, when interest in them has waned, there is little cactophilic Drosophila have taught us much about mainte- understanding of the particular alleles contained within them nance of genetic variability, host specificity, chemical ecol- that alone or in relation to other genes influence any com- ogy, trophic adaptation, and life history evolution (Barker ponent of fitness. Perhaps the most important rule about in- and Starmer 1982; Barker et al. 1990). Despite the fact that version polymorphisms learned from a long history of lab- 48% (44191) of all described and undescribed D. repleta oratory experiments (cf. Lewontin et al. 1981) is their sen- group species possess inversion polymorphism (Wasserman sitivity to environmental features such as temperature and 1992), in only a few species has there been concerted effort nutrition. -
Introduction and Bibliography
Pacific Science (1988), vol. 42, nos. 1-2 © 1988 by the University of Hawaii Press. All rights reserved Introduction and Bibliography Hampton Carson first came to Hawaii in LITERATURE CITED June 1963 at the urging of Elmo Hardy and Wilson Stone. That year saw the first major CARSON, H. L. 1980. Hypotheses that blur gathering in Honolulu of scientists from and grow. Pages 383-384 in E. Mayr and many specialties in the interdisciplinary and W. B. Provine, eds. The evolutionary syn cooperative pattern that has proved so pro thesis: Perspectives on the unification of ductive in the study of Hawaiian Drosophila biology. Harvard Univ. Press, Cambridge, on "the Project" (Spieth 1980). Carson came Mass . with Harrison Stalker from Washington SPIETH, H. T. 1980. Hawaiian Drosophila University in St. Louis. Together (following Project. Proc., Hawaiian Entomol. Soc. Dobzhansky), they had developed a power 23(2) :275-291. ful method of population studies based on detailed examination of the distribution of inversions in the polytene chromosomes of Bibliography ofHampton Lawrence Carson Drosophila robusta and other species of the 1934-1986 mesic forests of the central and eastern United States. 1. CARSON, H. L. 1934. Labrador quarry. Carson's cytological approach can be traced General Mag. 37(1) :97-104. to the influence of McClung, and especially 2. CARSON, H. L. 1935. Use of medicinal of Metz, during his graduate studies at the herbs among the Labrador Eskimo. University of Pennsylvania in the late 1930s General Mag. 37(4):436-439. and early 1940s (Carson 1980). These studies 3. CARSON, H . L. -
Cataglyphis, an Ant from the Sahara Desert WALTER J
Proc. Natl. Acad. Sci. USA Vol. 92, pp. 2994-2998, March 1995 Ecology Heat shock protein synthesis and thermotolerance in Cataglyphis, an ant from the Sahara desert WALTER J. GEHRING*t AND RUDIGER WEHNERt *Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland; and *Zoological Institute, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland Contributed by Walter J. Gehring, December 30, 1994 ABSTRACT The ant Cataglyphis lives in the Sahara desert cognates (HSCs), which are strongly expressed at normal and is one of the most thermotolerant land animals known. It temperatures (5, 6). In contrast to HSP70, the HSP83 protein forages at body temperatures above 50°C, and the critical is not only heat inducible, but it is also expressed at relatively thermal maxima are at 53.6 + O.8AC for Cataglyphis bombycina high levels during development at normal temperatures. The and 55.1 + 1.1°C for Cataglyphis bicolor. The synthesis and four small HSPs, HSP22, -23, -26, and -27, are encoded by four accumulation of heat shock proteins (HSPs) were analyzed in genes clustered at the same chromosomal locus. They are heat Cataglyphis and compared to Formica, an ant living in more inducible in all cells but are also expressed in various tissues moderate climates, and to two Drosophila species. In Catagly- during development at normal temperatures. In contrast to HSP70 and HSP83, which are highly conserved in evolution, phis, protein synthesis continues at temperatures up to 45°C the four small HSPs are more variable among various Dro- as compared to 39°C for Formica and Drosophila. -
Ant Community Potential for Pest Control in Olive Groves: Management and Landscape
bioRxiv preprint doi: https://doi.org/10.1101/2020.05.18.103028; this version posted May 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Title: 2 Ant community potential for pest control in olive groves: management and landscape 3 effects 4 5 Authors 6 Carlos Martínez-Núñez 1*, Pedro J. Rey1,2, Teresa Salido1, Antonio, J. Manzaneda1,3, 7 Francisco M. Camacho1 and Jorge Isla4 8 1 Dept. Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén. E-23071 Jaén, 9 Spain. 10 2 Instituto Interuniversitario del Sistema Tierra de Andalucía, Universidad de Jaén, E-23071 11 Jaén, Spain. 12 3 Centro de Estudios Avanzados en Ciencias de la Tierra, Energía y Medio Ambiente. 13 Universidad de Jaén, E-23071 Jaén, Spain. 14 4 Integrative Ecology Group, Estación Biológica de Doñana, EBD-CSIC, Sevilla, Spain. 15 16 *Corresponding author: [email protected] 17 Declaration of interest: None 18 19 20 bioRxiv preprint doi: https://doi.org/10.1101/2020.05.18.103028; this version posted May 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 21 Abstract 22 Ants are important pest control agents in many agroecosystems worldwide. However, little is 23 known about how management, ecological contrast generated by different agricultural practices, 24 and landscape complexity affect their communities and the potential pest control that they can 25 provide. -
Cataglyphis, an Ant from the Sahara Desert WALTER J
Proc. Natl. Acad. Sci. USA Vol. 92, pp. 2994-2998, March 1995 Ecology Heat shock protein synthesis and thermotolerance in Cataglyphis, an ant from the Sahara desert WALTER J. GEHRING*t AND RUDIGER WEHNERt *Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland; and *Zoological Institute, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland Contributed by Walter J. Gehring, December 30, 1994 ABSTRACT The ant Cataglyphis lives in the Sahara desert cognates (HSCs), which are strongly expressed at normal and is one of the most thermotolerant land animals known. It temperatures (5, 6). In contrast to HSP70, the HSP83 protein forages at body temperatures above 50°C, and the critical is not only heat inducible, but it is also expressed at relatively thermal maxima are at 53.6 + O.8AC for Cataglyphis bombycina high levels during development at normal temperatures. The and 55.1 + 1.1°C for Cataglyphis bicolor. The synthesis and four small HSPs, HSP22, -23, -26, and -27, are encoded by four accumulation of heat shock proteins (HSPs) were analyzed in genes clustered at the same chromosomal locus. They are heat Cataglyphis and compared to Formica, an ant living in more inducible in all cells but are also expressed in various tissues moderate climates, and to two Drosophila species. In Catagly- during development at normal temperatures. In contrast to HSP70 and HSP83, which are highly conserved in evolution, phis, protein synthesis continues at temperatures up to 45°C the four small HSPs are more variable among various Dro- as compared to 39°C for Formica and Drosophila. -
On Some Population Parameters of Drosophilids in Switzerland (Diptera, Drosophilidae)
Bächli, G; Schatzmann, E; Haring, E (2008). On some population parameters of drosophilids in Switzerland (Diptera, Drosophilidae). Mitteilungen- Schweizerische Entomologische Gesellschaft , 81:243-260. Postprint available at: http://www.zora.uzh.ch University of Zurich Posted at the Zurich Open Repository and Archive, University of Zurich. Zurich Open Repository and Archive http://www.zora.uzh.ch Originally published at: Mitteilungen- Schweizerische Entomologische Gesellschaft 2008, 81:243-260. Winterthurerstr. 190 CH-8057 Zurich http://www.zora.uzh.ch Year: 2008 On some population parameters of drosophilids in Switzerland (Diptera, Drosophilidae) Bächli, G; Schatzmann, E; Haring, E Bächli, G; Schatzmann, E; Haring, E (2008). On some population parameters of drosophilids in Switzerland (Diptera, Drosophilidae). Mitteilungen- Schweizerische Entomologische Gesellschaft , 81:243-260. Postprint available at: http://www.zora.uzh.ch Posted at the Zurich Open Repository and Archive, University of Zurich. http://www.zora.uzh.ch Originally published at: Mitteilungen- Schweizerische Entomologische Gesellschaft 2008, 81:243-260. MITTEILUNGEN DER SCHWEIZERISCHEN ENTOMOLOGISCHEN GESELLSCHAFT BULLETIN DE LA SOCIÉTÉ ENTOMOLOGIQUE SUISSE 81: 243–260, 2008 On some population parameters of drosophilids in Switzerland (Diptera, Drosophilidae) GERHARD BÄCHLI1, ERNST SCHATZMANN2 & ELISABETH HARING3 1 Zoological Museum, Winterthurerstrasse 190, CH-8047 Zürich; [email protected] 2 Hofwisenstrasse 3, CH-8317 Tagelswangen; [email protected] 3 Museum of Natural History, 1. Zoological Department, Molecular Systematics, Burgring 7, A-1010 Vienna; [email protected] Based on a collection of Drosophilidae in western Switzerland, the counts of flies were analyzed to get coordinated information about population parameters such as aggregation, succession, sex ratio, association and diurnal activity.