Supplemental Table 2. Expression profile of cell cycle genes in FOXF1 siRNA-transfected BT549 cells vs. control siRNA-transfected cells siControl siFOXF1 siControl siFOXF1 siControl siFOXF1 Gene symbol Average Ct Average Ct AVG Delta(Ct) (Ct(GOI)AVG - AveDelta(Ct) Ct(HKG)) (Ct(GOI)2^(-Avg.(Delta(Ct)) - Ave Ct(HKG)) 2^(-Avg.(Delta(Ct)) A01 ABL1 20.391 21.0059 0.55892 0.65368 0.67881013 0.635656822 A02 ANAPC2 32.7951 38.8081 12.96302 18.45588 0.00012524 2.78116E-06 A03 ANAPC4 24.797 27.2979 4.96492 6.94568 0.032019176 0.008112262 A04 DIRAS3 Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined A05 ATM 31.4392 32.8151 11.60712 12.46288 0.00032056 0.000177133 A06 ATR 22.1905 22.2736 2.35842 1.92138 0.195004592 0.26400186 A07 BAX 21.7453 21.5838 1.91322 1.23158 0.265499307 0.42585081 A08 BCCIP 22.4774 23.2689 2.64532 2.91668 0.159837741 0.132431663 A09 BCL2 Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined A10 BIRC5 32.4976 31.663 12.66552 11.31078 0.000153921 0.000393655 A11 BRCA1 26.31 30.093 6.47792 9.74078 0.011218939 0.001168781 A12 BRCA2 25.3534 25.4835 5.52132 5.13128 0.021772939 0.028531907 B01 CCNB1 15.6774 15.1967 -4.15468 -5.15552 17.81079489 35.64233627 B02 CCNB2 20.1845 19.4191 0.35242 -0.93312 0.783269127 1.909400841 B03 CCNC 19.3827 19.6594 -0.44938 -0.69282 1.365453325 1.616440047 B04 CCND1 Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined B05 CCND2 29.8269 29.5986 9.99482 9.24638 0.000980075 0.001646502 B06 CCNE1 22.2102 22.1076 2.37812 1.75538 0.192359902 0.296195166 B07 CCNF 27.5356 27.1297 7.70352 6.77748 0.004797438 0.009115389 B08 CCNG1 18.6415 19.3194 -1.19058 -1.03282 2.282444848 2.046019649 B09 CCNG2 27.2948 28.1258 7.46272 7.77358 0.005668882 0.004570032 B10 CCNH 19.088 19.0514 -0.74408 -1.30082 1.674905849 2.463688742 B11 CCNT1 27.314 29.2946 7.48192 8.94238 0.005593938 0.00203271 B12 CCNT2 23.1953 23.2571 3.36322 2.90488 0.097178434 0.133519281 C01 CDC16 18.847 19.2198 -0.98508 -1.13242 1.979423072 2.192261655 C02 CDC2 17.5007 18.1826 -2.33138 -2.16962 5.032865349 4.499048752 C03 CDC20 18.4535 18.4698 -1.37858 -1.88242 2.60012323 3.686929934 C04 CDC34 34.6153 34.0491 14.78322 13.69688 3.54656E-05 7.53058E-05 C05 CDK2 19.058 19.3012 -0.77408 -1.05102 1.710099181 2.071994251 C06 CDK4 21.036 21.5722 1.20392 1.21998 0.434094184 0.429288669 C07 CDK5R1 34.2815 33.7238 14.44942 13.37158 4.46983E-05 9.43525E-05 C08 CDK5RAP1 27.7663 27.208 7.93422 6.85578 0.004088479 0.008633852 C09 CDK6 20.0366 21.7226 0.20452 1.37038 0.867827375 0.386789356 C10 CDK7 19.6398 19.8845 -0.19228 -0.46772 1.142567976 1.382922204 C11 CDK8 28.2742 27.9151 8.44212 7.56288 0.002875204 0.005288668 C12 CDKN1A 20.3262 20.1767 0.49412 -0.17552 0.709994621 1.129371398 D01 CDKN1B 27.624 27.3991 7.79192 7.04688 0.004512304 0.007562715 D02 CDKN2A 17.6365 18.178 -2.19558 -2.17422 4.580737845 4.513416759 D03 CDKN2B 24.5389 25.7537 4.70682 5.40148 0.038291819 0.023658788 D04 CDKN3 18.062 17.8909 -1.77008 -2.46132 3.410728693 5.507203808 D05 CHEK1 19.7164 20.0825 -0.11568 -0.26972 1.083485615 1.205573826 D06 CHEK2 21.0729 21.1993 1.24082 0.84708 0.423132088 0.555908752 D07 CKS1B Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined D08 CKS2 17.7676 18.9019 -2.06448 -1.45032 4.182831842 2.732686576 D09 CUL1 19.9308 19.9251 0.09872 -0.42712 0.933861172 1.344546826 D10 CUL2 19.4667 19.3871 -0.36538 -0.96512 1.288220897 1.952225896 D11 CUL3 35.485 39.9909 15.65292 19.63868 1.94089E-05 1.22509E-06 D12 DDX11 29.9368 29.871 10.10472 9.51878 0.000908189 0.001363207 E01 DNM2 32.4104 32.3534 12.57832 12.00118 0.000163512 0.000243941 E02 E2F4 30.9643 31.2912 11.13222 10.93898 0.000445521 0.000509376 E03 GADD45A Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined E04 GTF2H1 21.4486 21.3144 1.61652 0.96218 0.32612117 0.513280729 E05 GTSE1 22.0112 21.7734 2.17912 1.42118 0.220810396 0.373406773 E06 HERC5 29.2313 29.0098 9.39922 8.65758 0.001480992 0.002476331 E07 HUS1 Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined E08 KNTC1 21.8068 21.7638 1.97472 1.41158 0.254419296 0.375899786 E09 KPNA2 36.6913 33.7573 16.85922 13.40508 8.41142E-06 9.21868E-05 E10 MAD2L1 18.1565 18.2572 -1.67558 -2.09502 3.194477548 4.272320859 E11 MAD2L2 35.0533 34.8664 15.22122 14.51418 2.61792E-05 4.27363E-05 E12 MCM2 23.1059 24.4197 3.27382 4.06748 0.10339082 0.059643966 F01 MCM3 37.3741 33.013 17.54202 12.66078 5.23993E-06 0.000154428 F02 MCM4 18.2862 18.5102 -1.54588 -1.84202 2.919821147 3.585116498 F03 MCM5 21.784 21.9788 1.95192 1.62658 0.258472016 0.323855017 F04 MKI67 19.2416 19.175 -0.59048 -1.17722 1.505747642 2.261405955 F05 MNAT1 19.6929 19.7648 -0.13918 -0.58742 1.101278992 1.50255729 F06 MRE11A 30.9678 32.5749 11.13572 12.22268 0.000444441 0.000209222 F07 NBN Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined F08 PCNA 17.4911 17.7371 -2.34098 -2.61512 5.066466778 6.126741575 F09 RAD1 21.7625 21.9925 1.93042 1.64028 0.262352783 0.320794208 F10 RAD17 21.0312 20.6359 1.19912 0.28368 0.435540867 0.821492894 F11 RAD51 29.9035 30.6412 10.07142 10.28898 0.000929395 0.000799297 F12 RAD9A 26.4463 26.3916 6.61422 6.03938 0.010207547 0.015204266 G01 RB1 39.5 35.0395 19.66792 14.68728 1.20051E-06 3.79043E-05 G02 RBBP8 18.9719 19.1509 -0.86018 -1.20132 1.815264781 2.299499685 G03 RBL1 21.1005 21.3763 1.26842 1.02408 0.415114146 0.491723769 G04 RBL2 Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined G05 RPA3 19.5674 19.4371 -0.26468 -0.91512 1.201369548 1.885725916 G06 SERTAD1 24.4933 24.1882 4.66122 3.83598 0.039521459 0.070025297 G07 SKP2 20.4889 21.0351 0.65682 0.68288 0.634274831 0.622920519 G08 SUMO1 22.3272 22.0501 2.49512 1.69788 0.177375665 0.308238719 G09 TFDP1 24.9559 24.8139 5.12382 4.46168 0.028679824 0.045383762 G10 TFDP2 23.5693 24.8361 3.73722 4.48388 0.074986776 0.044690748 G11 TP53 35.0331 36.1395 15.20102 15.78728 2.65483E-05 1.7683E-05 G12 UBA1 Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined H01 B2M 15.8312 16.2871 -4.00088 -4.06512 16.00976249 16.73875127 H02 HPRT1 27.69 28.22 7.85792 7.86778 0.004310527 0.004281168 H03 RPL13A 16.8619 17.5346 -2.97018 -2.81762 7.836340032 7.049984082 H04 GAPDH 13.4913 14.0455 -6.34078 -6.30672 81.05223145 79.16111346 H05 ACTB 25.286 25.6739 5.45392 5.32168 0.022814265 0.0250043 siControl siFOXF1 Log10 (2^(-Avg.(Delta(Ct)))Log10 (2^(-Avg.(Delta(Ct)))Fold change Gene description -0.168251685 -0.196777288 -1.067887744 C-abl oncogene 1, receptor tyrosine kinase -3.902257854 -5.555773476 -45.03141792 Anaphase promoting complex subunit 2 -1.494589846 -2.09085802 -3.947009523 Anaphase promoting complex subunit 4 Undetermined Undetermined Undetermined DIRAS family, GTP-binding RAS-like 3 -3.494091283 -3.751700712 -1.809711846 Ataxia telangiectasia mutated -0.709955162 -0.578393013 1.353823814 Ataxia telangiectasia and Rad3 related -0.575936608 -0.370742522 1.603962041 BCL2-associated X protein -0.796320668 -0.878008168 -1.206945055 BRCA2 and CDKN1A interacting protein Undetermined Undetermined Undetermined B-cell CLL/lymphoma 2 -3.812701431 -3.404884054 2.557510211 Baculoviral IAP repeat-containing 5 -1.95004823 -2.932266961 -9.598839537 Breast cancer 1, early onset -1.662082936 -1.544669196 1.310429736 Breast cancer 2, early onset 1.250683302 1.551966163 2.001164826 Cyclin B1 -0.106088991 0.28089711 2.437732799 Cyclin B2 0.135276859 0.208559602 1.183812011 Cyclin C Undetermined Undetermined Undetermined Cyclin D1 -3.008740621 -2.783437731 1.679975275 Cyclin D2 -0.715885453 -0.528422034 1.539796824 Cyclin E1 -2.318990592 -2.040224775 1.900053446 Cyclin F 0.358400292 0.3109098 -1.115553729 Cyclin G1 -2.246502569 -2.340080754 -1.240446918 Cyclin G2 0.223990399 0.391585839 1.470941631 Cyclin H -2.252282345 -2.691924613 -2.751960953 Cyclin T1 -1.012430102 -0.874456014 1.373959996 Cyclin T2 0.296538628 0.340892388 1.107525564 Cell division cycle 16 homolog (S. cerevisiae) 0.701815311 0.653120699 -1.118650992 Cell division cycle 2, G1 to S and G2 to M 0.414993931 0.566664884 1.417982768 Cell division cycle 20 homolog (S. cerevisiae) -4.450192652 -4.123171727 2.123346768 Cell division cycle 34 homolog (S. cerevisiae) 0.233021299 0.316388546 1.211622269 Cyclin-dependent kinase 2 -0.362416032 -0.367250574 -1.011194134 Cyclin-dependent kinase 4 -4.34970884 -4.025246669 2.110873319 Cyclin-dependent kinase 5, regulatory subunit 1 (p35) -2.388438212 -2.063795424 2.111751389 CDK5 regulatory subunit associated protein 1 -0.061566655 -0.412525485 -2.243669223 Cyclin-dependent kinase 6 0.057882048 0.14079775 1.210363175 Cyclin-dependent kinase 7 -2.541331347 -2.276653734 1.839406062 Cyclin-dependent kinase 8 -0.148744941 0.052836785 1.590675992 Cyclin-dependent kinase inhibitor 1A (p21, Cip1) -2.345601644 -2.121322256 1.676020738 Cyclin-dependent kinase inhibitor 1B (p27, Kip1) 0.660935438 0.654505437 -1.01491577 Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) -1.416894004 -1.626007501 -1.618502955 Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) 0.532847175 0.740931149 1.614670736 Cyclin-dependent kinase inhibitor 3 0.03482315 0.08119381 1.11268097 CHK1 checkpoint homolog (S. pombe) -0.373524039 -0.254996489 1.313794836 CHK2 checkpoint homolog (S. pombe) Undetermined Undetermined Undetermined CDC28 protein kinase regulatory subunit 1B 0.621470405 0.436589823 -1.530666517 CDC28 protein kinase regulatory subunit 2 -0.029717681 0.128575932 1.439771634 Cullin 1 0.10999034 0.290530069 1.515443431 Cullin 2 -4.71199844 -5.911831755 -15.84285019 Cullin 3 -3.041823818 -2.865438302 1.501016671 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) -3.786451615 -3.612715163 1.491888793 Dynamin 2 -3.351132138 -3.292961102 1.143328518 E2F transcription factor 4, p107/p130-binding Undetermined Undetermined Undetermined Growth arrest and DNA-damage-inducible, alpha -0.486621009 -0.289645041 1.573895767 General transcription factor IIH, polypeptide 1, 62kDa -0.655980484 -0.427817809 1.691074243 G-2 and S-phase expressed 1 -2.829447156 -2.60619127 1.67207551 Hect domain and RLD 5 Undetermined Undetermined Undetermined HUS1 checkpoint homolog (S. pombe) -0.594449953 -0.424927921 1.477481432 Kinetochore associated 1 -5.075130923 -4.035331174 10.95972733 Karyopherin alpha 2 (RAG cohort 1, importin alpha 2) 0.50439984 0.630663862 1.337408322 MAD2 mitotic arrest deficient-like 1 (yeast) -4.582043791 -4.369203542 1.632451353 MAD2 mitotic arrest deficient-like 2 (yeast) -0.98551802 -1.224433487 -1.733466553 Minichromosome maintenance complex component 2 -5.280674205 -3.811274549 29.47132461 Minichromosome maintenance complex component 3 0.46535625 0.554503273 1.22785483 Minichromosome maintenance complex component 4 -0.587586469 -0.48964937 1.252959689 Minichromosome maintenance complex component 5 0.177752192 0.354378531 1.501849242 Antigen identified by monoclonal antibody Ki-67 0.041897355 0.17683104 1.364374787 Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) -3.352185743 -3.679393307 -2.124259475 MRE11 meiotic recombination 11 homolog A (S. cerevisiae) Undetermined Undetermined Undetermined Nibrin 0.704705199 0.787229562 1.209273019 Proliferating cell nuclear antigen -0.581114324 -0.493773481 1.222758929 RAD1 homolog (S. pombe) -0.360971088 -0.085396189 1.886144229 RAD17 homolog (S. pombe) -3.031799519 -3.097291605 -1.162765362 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) -1.991078618 -1.818034535 1.489512262 RAD9 homolog A (S. pombe) -5.920633872 -4.421311835 31.57344987 Retinoblastoma 1 0.258939982 0.361633354 1.266757175 Retinoblastoma binding protein 8 -0.381832467 -0.308278798 1.184550742 Retinoblastoma-like 1 (p107) Undetermined Undetermined Undetermined Retinoblastoma-like 2 (p130) 0.079676619 0.27547857 1.569646841 Replication protein A3, 14kDa -1.403167036 -1.154745043 1.771829767 SERTA domain containing 1 -0.197722522 -0.205567363 -1.018227546 S-phase kinase-associated protein 2 (p45) -0.751105963 -0.511112809 1.737773434 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) -1.542423512 -1.343099511 1.582428155 Transcription factor Dp-1 -1.12501532 -1.349782377 -1.677903796 Transcription factor Dp-2 (E2F dimerization partner 2) -4.575962985 -4.75244483 -1.501349644 Tumor protein p53 Undetermined Undetermined Undetermined Ubiquitin-like modifier activating enzyme 1 1.204384889 1.223723056 1.045534016 Beta-2-microglobulin -2.365469624 -2.368437779 -1.006857839 Hypoxanthine phosphoribosyltransferase 1 0.894113273 0.848188136 -1.111540103 Ribosomal protein L13a 1.908764976 1.898511894 -1.023889482 Glyceraldehyde-3-phosphate dehydrogenase -1.641793514 -1.601985307 1.095994076 Actin, beta