Microbial Morphology and Community Structure in a Suspended Carrier Biofilm Reactor As a Function of Substrate Loading Rates
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Response of Heterotrophic Stream Biofilm Communities to a Gradient of Resources
The following supplement accompanies the article Response of heterotrophic stream biofilm communities to a gradient of resources D. J. Van Horn1,*, R. L. Sinsabaugh1, C. D. Takacs-Vesbach1, K. R. Mitchell1,2, C. N. Dahm1 1Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA 2Present address: Department of Microbiology & Immunology, University of British Columbia Life Sciences Centre, Vancouver BC V6T 1Z3, Canada *Email: [email protected] Aquatic Microbial Ecology 64:149–161 (2011) Table S1. Representative sequences for each OTU, associated GenBank accession numbers, and taxonomic classifications with bootstrap values (in parentheses), generated in mothur using 14956 reference sequences from the SILVA data base Treatment Accession Sequence name SILVA taxonomy classification number Control JF695047 BF8FCONT18Fa04.b1 Bacteria(100);Proteobacteria(100);Gammaproteobacteria(100);Pseudomonadales(100);Pseudomonadaceae(100);Cellvibrio(100);unclassified; Control JF695049 BF8FCONT18Fa12.b1 Bacteria(100);Proteobacteria(100);Alphaproteobacteria(100);Rhizobiales(100);Methylocystaceae(100);uncultured(100);unclassified; Control JF695054 BF8FCONT18Fc01.b1 Bacteria(100);Planctomycetes(100);Planctomycetacia(100);Planctomycetales(100);Planctomycetaceae(100);Isosphaera(50);unclassified; Control JF695056 BF8FCONT18Fc04.b1 Bacteria(100);Proteobacteria(100);Gammaproteobacteria(100);Xanthomonadales(100);Xanthomonadaceae(100);uncultured(64);unclassified; Control JF695057 BF8FCONT18Fc06.b1 Bacteria(100);Proteobacteria(100);Betaproteobacteria(100);Burkholderiales(100);Comamonadaceae(100);Ideonella(54);unclassified; -
Community and Whole Genome Analysis of Wastewater Bacteria
Community and Whole Genome Analysis of Wastewater Bacteria By Daniel Rice BSc(Hons) A thesis submitted in total fulfilment of the requirements for the degree of Master of Science School of Life Sciences College of Science, Health and Engineering La Trobe University Victoria Australia February 2020 1 TABLE OF CONTENTS TABLE OF CONTENTS ................................................................................................................ i LIST OF FIGURES ..................................................................................................................... iii LIST OF TABLES ....................................................................................................................... iv LIST OF ABBREVIATIONS .......................................................................................................... v STATEMENT OF AUTHORSHIP ................................................................................................vii ACKNOWLEDGEMENTS ......................................................................................................... viii SUMMARY ..............................................................................................................................ix SECTION 1: INTRODUCTION 1.1 Activated sludge process ................................................................................................... 2 1.2 Community profiling of activated sludge bacterial communities ....................................... 7 1.2.1 Phosphorous removal .......................................................................................... -
Hydrogenophaga Electricum Sp. Nov., Isolated from Anodic Biofilms of an Acetate-Fed Microbial Fuel Cell
J. Gen. Appl. Microbiol., 59, 261‒266 (2013) Full Paper Hydrogenophaga electricum sp. nov., isolated from anodic biofilms of an acetate-fed microbial fuel cell Zen-ichiro Kimura and Satoshi Okabe* Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060‒8628, Japan (Received October 25, 2012; Accepted April 2, 2013) A Gram-negative, non-spore-forming, rod-shaped bacterial strain, AR20T, was isolated from an- odic biofilms of an acetate-fed microbial fuel cell in Japan and subjected to a polyphasic taxo- nomic study. Strain AR20T grew optimally at pH 7.0‒8.0 and 25°C. It contained Q-8 as the pre- dominant ubiquinone and C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:02OH), and C18:1ω7c as the major fatty acids. The DNA G+C content was 67.1 mol%. A neighbor-joining phylogenetic tree revealed that strain AR20T clustered with three type strains of the genus Hydrogenophaga (H. flava, H. bisanensis and H. pseudoflava). Strain AR20T exhibited 16S rRNA gene sequence similarity values of 95.8‒97.7% to the type strains of the genus Hydrogenophaga. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain AR20T is considered a novel species of the genus Hydrogenophaga, for which the name Hydrogenophaga electricum sp. nov. is pro- posed. The type strain is AR20T (= KCTC 32195T = NBRC 109341T). Key Words—Hydrogenophaga electricum; hydrogenotrophic exoelectrogen; microbial fuel cell Introduction the MFC was analyzed. Results showed that bacteria belonging to the genera Geobacter and Hydrogenoph- Microbial fuel cells (MFCs) are devices that are able aga were abundantly present in the anodic biofilm to directly convert the chemical energy of organic community (Kimura and Okabe, 2013). -
Bioflocculation of Wastewater Organic Matter at Short Retention Times
Bioflocculation of Wastewater Organic Matter at Short Retention Times Lena Faust Thesis committee Promotor Prof. dr. ir. H.H.M. Rijnaarts Professor, Chair Environmental Technology Wageningen University Co-promotor Dr. ir. H. Temmink Assistant professor, Sub-department of Environmental Technology Wageningen University Other members Prof. Dr A.J.M. Stams, Wageningen University Prof. Dr. I. Smets, University of Leuven, Belgium Prof. Dr. B.-M. Wilen, Chalmers University of Technology, Sweden Prof. Dr. D.C. Nijmeijer, University of Twente, The Netherlands This research was conducted under the auspices of the Graduate School SENSE (Socio- Economic and Natural Sciences of the Environment) Bioflocculation of Wastewater Organic Matter at Short Retention Times Lena Faust Thesis submitted in fulfillment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr M.J. Kropff, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Wednesday 3rd of December 2014 at 1.30 p.m. in the Aula. Lena Faust Bioflocculation of Wastewater Organic Matter at Short Retention Times, 163 pages. PhD thesis, Wageningen University, Wageningen, NL (2014) With references, with summaries in English and Dutch ISBN 978-94-6257-171-6 ˮDa steh ich nun, ich armer Tor, und bin so klug als wie zuvor.ˮ Dr. Faust (in Faust I written by Johann Wolgang von Goethe) Contents 1 GENERAL INTRODUCTION ....................................................................................................................1 -
Genome Analysis of Two Bacterial Strains Isolated from Diseased
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 6 September 2020 doi:10.20944/preprints202009.0144.v1 1 Article 2 Genome analysis of two bacterial strains isolated 3 from diseased freshwater sponge reveals the 4 probable cause of its joint domination in microbial 5 community 6 Ivan Petrushin 1,2,*, Sergei Belikov 1 and Lubov Chernogor 1, 7 1 Limnological institute, Siberian Branch of the Russian Academy of Sciences, Irkutsk 664033, Russia; 8 [email protected] 9 2 Irkutsk State University, Irkutsk 664003, Russia; [email protected] 10 * Correspondence: [email protected]; 11 12 Abstract: Endemic freshwater sponges (Demosponges, Lubomirskiidae) dominate in Lake Baikal 13 and are multicellular filter-feeding animals represent a complex consortium of many species of 14 eukaryotes and prokaryotes. In recent years, mass disease and death of the L. baicalensis have been 15 an urgent problem of Lake Baikal. The etiology and ecology of these events remain unknown. 16 Bacteria in microbiomes of diseased sponges of the families Flavobacteriaceae and Oxalobacteraceae 17 were dominant. Both species are opportunistic pathogens common for freshwater ecosystems. The 18 aim of our study is to analyze the genomes of strains Janthinobacterium sp. SLB01 and Flavobacterium 19 sp. SLB02, isolated from diseased sponges to identify the reasons for their joint dominance. The first 20 one attacks the other cells using type VI secretion system, suppress gram-positive bacteria with 21 violacein pigment and regulate its own activity via quorum sensing. It makes the floc and strong 22 biofilm by exopolysaccharide biosynthesis and PEP‐CTERM proteins expression. The second one 23 utilizes the fragments of cell walls produced of polysaccharides. -
Rpon (Σ54) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola Tertiaricarbonis Strain
Lawrence Berkeley National Laboratory Recent Work Title RpoN (σ54) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola tertiaricarbonis Strain. Permalink https://escholarship.org/uc/item/9f26h2cp Journal Applied and environmental microbiology, 83(14) ISSN 0099-2240 Authors Yu, Dianzhen Xia, Ming Zhang, Liping et al. Publication Date 2017-07-01 DOI 10.1128/aem.00709-17 Peer reviewed eScholarship.org Powered by the California Digital Library University of California RpoN (σσ54) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola tertiaricarbonis Strain Dianzhen Yu,a,b Ming Xia,a,b Liping Zhang,a Yulong Song,a You Duan,a,b Tong Yuan,d,f Minjie Yao,c Liyou Wu,d Chunyuan Tian,e Zhenbin Wu,a Xiangzhen Li,c Jizhong Zhou,d Dongru Qiua Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Chinaa ; University of Chinese Academy of Sciences, Beijing, Chinab; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Chinac ; Institute for Environmental Genomics, Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma, USAd; School of Life Sciences and Technology, Hubei Engineering University, Xiaogan, Chinae; College of Life Science, Henan Agricultural University, Zhengzhou, Chinaf ABSTRACT Some bacteria are capable of forming flocs, in which bacterial cells become self-flocculated by secreted extracellular polysaccharides and other biopolymers. The floc-forming bacteria play a central role in activated sludge, which has been widely utilized for the treatment of municipal sewage and industrial wastewater. Here, we use a floc-forming bacterium, Aquincola tertiaricarbonis RN12, as a model to explore the biosynthesis of extracellular polysaccharides and the regulation of floc formation. -
55Th Annual W.W.O.A. Conference October 5-8, 2021 La Crosse Convention Center, La Crosse Inside This Issue… 2021- 2022 W.W.O.A
VOL. 241, JUNE 2021 WISCONSIN WASTEWATER OPERATORS’ ASSOCIATION, INC. Aerial view of Jefferson Wastewater Treatment Plant, Jefferson, Wisconsin 55th Annual W.W.O.A. Conference October 5-8, 2021 La Crosse Convention Center, La Crosse Inside This Issue… 2021- 2022 W.W.O.A. OFFICIAL DIRECTORY • Presidents message / Page 3 Don Lintner Jenny Pagel President Director (2021) N2511 State Rd 57 Wastewater Foreman • Tribute to Tim Nennig / Page 4 New Holstein, WI 53061 City of Clintonville Cell: 920-418-3869 N9055 Cty Road M [email protected] Shiocton WI 54170 • City of Jefferson Wastewater / Page 6 Work: 715-823-7675 Rick Mealy Cell: 920-606-4634 President Elect [email protected] Independent Contractor Lab • Board meeting minutes & Regulatory Assistance April 2 and 3, 2020 / Page 17 319 Linden Lane Marc Stephanie Delavan WI 53115 Director (2020) Cell: 608-220-9457 Director of Public Works • Collection System seminars / Page 24 [email protected] Village of Valders 1522 Puritan Rd New Holstein WI 53061 Jeremy Cramer Work: 920-629-4970 • Board meeting minutes Vice President Wastewater Treatment Cell: 920-251-8100 March 19, 2020 / Page 25 Director [email protected] City of Sun Prairie Joshua Voigt 300 E Main Street • Reminder: Director (2022) Sun Prairie WI 53590 Direct Sales Representative Awards nominations / Page 26 Work: 608-825-0731 Flygt a Xylem Brand Cell: 608-235-9280 3894 Lake Drive jcramer@ Hartford WI 53027 • Troubleshooting Corner: cityofsunprairie.com Work: 262-506-2343 Zoogloea and Thauera / Page 27 Cell: 414-719-5567 [email protected] Jeff Smudde • Index of advertisers / Page 30 Past President Nate Tillis Director of Environmental Director (2022) Programs Maintenance Supervisor NEW Water (GBMSD) City of Waukesha 2231 N Quincy St. -
Ample Arsenite Bio-Oxidation Activity in Bangladesh Drinking Water Wells: a Bonanza for Bioremediation?
microorganisms Article Ample Arsenite Bio-Oxidation Activity in Bangladesh Drinking Water Wells: A Bonanza for Bioremediation? 1,2 3 3 1 1, Zahid Hassan , Munawar Sultana , Sirajul I. Khan , Martin Braster , Wilfred F.M. Röling y and Hans V. Westerhoff 1,4,5,* 1 Department of Molecular Cell Biology, Faculty of Science, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands 2 Department of Genetic Engineering and Biotechnology, Jagannath University, Dhaka 1100, Bangladesh 3 Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh 4 Manchester Centre for Integrative Systems Biology (MCISB), School of Chemical Engineering and Analytical Sciences (SCEAS), the University of Manchester, Manchester M13 9PL, UK 5 Synthetic Systems Biology and Nuclear Organization, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands * Correspondence: h.v.westerhoff@vu.nl; Tel.: +31-205-987-230 Deceased 25 September 2015. y Received: 18 June 2019; Accepted: 31 July 2019; Published: 8 August 2019 Abstract: Millions of people worldwide are at risk of arsenic poisoning from their drinking water. In Bangladesh the problem extends to rural drinking water wells, where non-biological solutions are not feasible. In serial enrichment cultures of water from various Bangladesh drinking water wells, we found transfer-persistent arsenite oxidation activity under four conditions (aerobic/anaerobic; heterotrophic/autotrophic). This suggests that biological decontamination may help ameliorate the problem. The enriched microbial communities were phylogenetically at least as diverse as the unenriched communities: they contained a bonanza of 16S rRNA gene sequences. These related to Hydrogenophaga, Acinetobacter, Dechloromonas, Comamonas, and Rhizobium/Agrobacterium species. In addition, the enriched microbiomes contained genes highly similar to the arsenite oxidase (aioA) gene of chemolithoautotrophic (e.g., Paracoccus sp. -
DGGE) and PGR Cloning of 16S Rrna Genes
THE UNIVERSITY OF NEW SOUTH thesis/Dissertation Sheet Surname or Family name:LE Rrst ^ I Other narne/s: Abbreviation for degree as given in the University calendar: MSc | ScliooliBiotechnolo^^^^^^ and Biomolecular ScienoBS Faculty: Science Title:Community analysis and physiological characterisation of bacterial isolates from a nitrifying membrane bioreactor Abstract This thesis focuses on the identification of early colonisers on membrane surfaces used in wastewater treatment, as well as the physiological characterisation of bacterial cultures isolated from different micro- environments of a membrane bioreactor (MBR). The bacterial community composition of early biofilms on membrane surfaces under different hydrodynamic conditions (pressurised and non-pressurised) and of the activated sludge in an MBR were examined by culture-independent, molecular-based methods of PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and PGR cloning of 16S rRNA genes. A bench-scale, nitrifying MBR treating artificial waste was employed. The hollow fibre ultrafiltration membrane was made of polypropylene with an average pore diameter of 0.04 \im. Analysis of DGGE profiles of the sessile communities on membrane surfaces revealed that Tetrasphaera elongata species were important colonisers due to their ability to bind to membrane surfaces irrespective of the hydrodynamic context and exposure time. Interactions between isolates from the bioreactor and membrane surfaces were further investigated by characterising the physiological traits important in biofilm initiation and proliferation on membrane surfaces such as motility, auto-aggregation, co-aggregation, hydrophobicity and quorum sensing. Bacterial strains were isolated from floes and supernatant phases of the activated sludge as well as from pressurised membrane surfaces. Microbacterium sp. were prevalent in all culture collections. -
Cooperative Interaction of Janthinobacterium Sp. SLB01 and Flavobacterium Sp
International Journal of Molecular Sciences Article Cooperative Interaction of Janthinobacterium sp. SLB01 and Flavobacterium sp. SLB02 in the Diseased Sponge Lubomirskia baicalensis Ivan Petrushin 1,2,* , Sergei Belikov 1 and Lubov Chernogor 1 1 Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Irkutsk 664033, Russia; [email protected] (S.B.); [email protected] (L.C.) 2 Faculty of Business Communication and Informatics, Irkutsk State University, Irkutsk 664033, Russia * Correspondence: [email protected] Received: 31 August 2020; Accepted: 25 October 2020; Published: 30 October 2020 Abstract: Endemic freshwater sponges (demosponges, Lubomirskiidae) dominate in Lake Baikal, Central Siberia, Russia. These sponges are multicellular filter-feeding animals that represent a complex consortium of many species of eukaryotes and prokaryotes. In recent years, mass disease and death of Lubomirskia baicalensis has been a significant problem in Lake Baikal. The etiology and ecology of these events remain unknown. Bacteria from the families Flavobacteriaceae and Oxalobacteraceae dominate the microbiomes of diseased sponges. Both species are opportunistic pathogens common in freshwater ecosystems. The aim of our study was to analyze the genomes of strains Janthinobacterium sp. SLB01 and Flavobacterium sp. SLB02, isolated from diseased sponges to identify the reasons for their joint dominance. Janthinobacterium sp. SLB01 attacks other cells using a type VI secretion system and suppresses gram-positive bacteria with violacein, and regulates its own activity via quorum sensing. It produces floc and strong biofilm by exopolysaccharide biosynthesis and PEP-CTERM/XrtA protein expression. Flavobacterium sp. SLB02 utilizes the fragments of cell walls produced by polysaccharides. These two strains have a marked difference in carbohydrate acquisition. -
Microbial Community in Hyperalkaline Steel Slag-Fill Emulates Serpentinizing Springs
diversity Article Microbial Community in Hyperalkaline Steel Slag-Fill Emulates Serpentinizing Springs J. Ingemar Ohlsson 1 , Jay T. Osvatic 2 , Eric D. Becraft 3 and Wesley D. Swingley 1,* 1 Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA 2 Centre for Microbiology and Environmental Systems Science, Division of Microbial Ecology, University of Vienna, 1090 Vienna, Austria 3 University of North Alabama, Florence, AL 35632, USA * Correspondence: [email protected]; Tel.: +1-209-777-4653 Received: 21 May 2019; Accepted: 21 June 2019; Published: 30 June 2019 Abstract: To date, a majority of studies of microbial life in hyperalkaline settings focus on environments that are also highly saline (haloalkaline). Haloalkaline conditions offer microbes abundant workarounds to maintain pH homeostasis, as salt ions can be exchanged for protons by dedicated antiporter proteins. Yet hyperalkaline freshwater systems also occur both naturally and anthropogenically, such as the slag fill aquifers around former Lake Calumet (Chicago, IL, USA). In this study, 16S rRNA gene sequences and metagenomic sequence libraries were collected to assess the taxonomic composition and functional potential of microbes present in these slag-polluted waterways. Relative 16S rRNA gene abundances in Calumet sediment and water samples describe community compositions not significantly divergent from those in nearby circumneutral conditions. Major differences in composition are mainly driven by Proteobacteria, primarily one sequence cluster closely related to Hydrogenophaga, which comprises up to 85% of 16S rRNA gene abundance in hyperalkaline surface sediments. Sequence identity indicates this novel species belongs to the recently established genus Serpentinomonas, a bacterial lineage associated with natural freshwater hyperalkaline serpentinizing springs. -
Microbial Diversity of a Remote Aviation Fuel Contaminated
ntal & A me na n ly o t ir ic v a Udotong et al., J Environ Anal Toxicol 2015, 5:6 n l T E o Journal of f x o i l c DOI: 10.4172/2161-0525.1000320 o a n l o r g u y o J Environmental & Analytical Toxicology ISSN: 2161-0525 ResearchResearch Article Article OpenOpen Access Access Microbial Diversity of a Remote Aviation Fuel Contaminated Sediment of a Lentic Ecosystem in Ibeno, Nigeria Udotong IR1*, Uko MP1 and Udotong JIR2 1Department of Microbiology, University of Uyo, Uyo, Akwa Ibom State, Nigeria 2Department of Biochemistry, University of Uyo, Uyo, Akwa Ibom State, Nigeria Abstract Environmental pollution from Oil & Gas Exploration & Production (O&G E&P) activities remains one of the major problems in the oil-producing communities of Nigeria. This results from improper oily wastes disposal as well as incessant oil spills in the region. The operator’s lack of responsible business practices in wastes management and the over-dependence of the economy on oil and gas earnings, in the most part, exacerbate the problems of environmental pollution. Government's lack of political will power which treats issues on oil pollution with levity and long period of neglect of these polluted sites leave the environment ecologically destabilized. Studies to ascertain the ecological status of remote aviation fuel-contaminated sediment of a lentic ecosystem in Inua Eyet Ikot village, Ibeno, Nigeria, have been carried out using conventional microbiological culture-dependent methods. This methodology is known to reveal only <1% of the microbial diversity present. These results were therefore considered inaccurate and grossly misleading.