9. Danish Conference on Biotechnology and Molecular Biology May 22-23, 2014 Hotel Munkebjerg, Vejle Animal Cell Cultures - Expression and Engineering Animal Cell Cultures - Expression and Engineering Front page image http://scitechdaily.com/microfluidic-system-precisely-measures-mammalian-cell-growth-rates/ 2 9. Danish Conference on Biotechnology and Molecular Biology May 22-23, 2014 Hotel Munkebjerg, Vejle Animal Cell Cultures - Expression and Engineering Over the last fifty years research in cell biotechnology has revealed substantial potentials for the production of bioactive proteins and use of the cells themselves particularly in medical applications. The development of efficient and safe processes for production of novel pharmaceuticals is of significant industrial importance and subject to extensive research efforts. The increasing availability of genome editing tools and genome sequences of mammalian cell factories, such as the Chinese hamster ovary cell, and other animal cell cultures enables for the first time a systems biotechnology driven approach to cell factory design. The conference will focus on - Perspectives in Animal Cell Factory Research - Expression and production systems - Technology platforms - Systems biology - Post translational modifications - Mammalian Cell Factories - Insect Cell Factories - Commercial perspectives and regulatory issues The conference includes a poster session covering a broad range of topics on biotechnology as well as a commercial exhibition of equipment, consumables and services to Danish biotechnology. 3 Animal Cell Cultures - Expression and Engineering Organisers Danish Biotechnological Society (DBS) is a scientific society established in 2006 in collaboration between Danish Biotechnology Forum and The Danish Society for Biochemistry and Molecular Biology. DBS is organised as part of The Danish Society of Engineers, IDA. The purpose of DBS is to facilitate networking within the field of biotechnology in Denmark, creating links between universities, research institutions, hospitals and companies. DBS represents professionals working with biotechnology and seeks to promote and communicate important issues on biotechnology, biochemistry and molecular biology to the public. DBS organises scientific conferences and meetings on biotechnology. Working group members - Anders Vagnø Pedersen, ALK-Abelló A/S - Catharina Stenholm, Novozymes A/S - Inge Kjærbølling, Society for Biological Engineering at Technical University of Denmark - Holti Kellezi, Synapse, University of Copenhagen - Jens Gram, CMC Biologics - Jochen Förster, Technical University of Denmark - Lars Haastrup Pedersen, Aalborg University - Leif Schauser, CLC Bio - Nils Joakim Færgeman, University of Southern Denmark - Steen Gammeltoft, Glostrup Hospital - Stephanie Mesker, Technical University of Denmark - Thomas Schou Larsen, Biopeople – Innovation - Uffe Hasbro Mortensen, Technical University of Denmark Co-organisers The conference is organised in collaboration with Danish Society of Biochemistry and Molecular Biology as well as BioPeople Secretariat Birgitte Magnér-Egeberg, IDA Netværkscenter, Kalvebod Brygge 31-33, DK-1780 Kbh. V. Tel: 33 18 46 46, e- mail: [email protected] 4 Program 5 Animal Cell Cultures - Expression and Engineering May 22 9.00 – 9.50 Registration, coffee and tea 9.50 – 10.00 Welcome by Lars Haastrup Pedersen, DBS, DK 10.00 – 11.00 Session 1 Perspectives in Animal Cell Factory Research chair: Lars Haastrup Pedersen, Aalborg University, DK 10.00 – 10.30 The genome area of CHO: from blackbox optimisation to designer cell lines? L1.1 Nicole Borth, University of Natural Resources and Life Sciences, AUT 10.30 – 11.00 Microparticles as cell-to-cell communicators to empower therapies and technology: L1.2 the case of megakaryocytic micro particles Terry Papoutsakis, University of Delaware, USA 11.00 – 13.00 Posters and exhibition, meet the speakers, and lunch 13.00 – 14.00 Session 2 Cell engineering and post-transcriptional mechanisms chair:, Thomas Schou Larsen, BioPeople, DK 13.00 – 13.30 Screening of small RNAs for impact on apoptosis and protein expression in cell cultures L2.1 Michael Betenbaugh, Johns Hopkins University ,USA 13.30 – 14.00 Efficient precise glycoengineering of cellular post translational modification pathways L2.2 Eric Paul Bennett, Copenhagen University, DK 14.00 – 15.00 Posters and exhibition, meet the speakers, hotel check in 15.00 – 16.00 Session 3 Expression and Production Systems chair: Jens Gram, CMC Biologics, DK 15.00 –15.30 The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments Jonathan D. Chesnut, Thermo Fisher Scientific, USA L3.1 15.30 – 16.00 Accelerating Genome Editing in CHO Cells using CRISPR CAS9 L3.2 Helene Faustrup Kildegård, Technical University of Denmark, DK 16.00 – 17.00 Posters and exhibition, meet the speakers 17.00 – 18.00 Session 3 cont. Expression and production in CHO cells chair: Leif Schauser, CLC Bio, DK 17.00 – 17.30 CHO cell engineering for improved therapeutic protein production L3.3 Gyun Min Lee, KAIST, KOR 17.30 – 18.00 Large-scale transient gene expression platforms using HEK293 and CHO cells for protein production L3.4 Yves Durocher, Animal Cell Technology Research Group, National Research Council, CAN 18.00 – 19.00 Posters and exhibition, meet the speakers 19.30 – Conference dinner 6 May 23 8.30 – 9.30 Session 4 Insect Cell Factories chair: Leif Schauser, CLC Bio, DK 8.30 – 9.00 Development of Drosophila S2 insect-cell based Malaria Vaccine production processes L4.1 Wian de Jongh, ExpreS2ion Biotechnologies, DK 9.00 – 9.30 The state-of-play of baculovirus-mediated protein expression in insect cells L4.2 Svend Kjær, London Research Institute, UK 9.30 – 10.30 Posters and exhibition, meet the speakers, hotel check out 10.30 – 11.45 Session 5 Mammalian Cell Culture Production Systems chair: Nils Færgeman, University of Southern Denmark, DK 10.30 – 10.55 Industrial production systems L5.1 Jens Gram, CMC Biologics, DK 10.55 – 11.20 Cell cultures on chip - possibilities and challenges L5.2 Martin Dufva, Department of Micro- and Nanotechnology, Technical University of Denmark, DK 11.20 – 11.45 Bioprocess cost modeling guiding early stage process development L5.3 Kai M. Touw, Crucell, NL 11.45 – 13.15 Lunch, posters and exhibition, meet the speakers 13.15 – 14.05 Session 6 Regulatory Issues and Commercial Perspectives chair: Thomas Schou Larsen, BioPeople, DK 13.15 – 13.40 Regulatory Environment of White Biotechnology with a Shade of Red L6.1 Carsten Hjort, Novozymes A/S, DK 13.40 – 14.05 Biological engineering – a stronghold in Danish biotech L6.2 Martin Bonde, Dansk Biotek 14.05 – 14.40 Posters and exhibition, meet the speakers 14.40 – 15.55 Session 7 Mammalian Cell Factories and Systems biology chair: Mikael Rørdam Andersen, Technical University of Denmark, DK 14.40 – 15.05 Engineering Biological Networks in CHO Cell Lines L7.1 Mikael Rørdam Andersen, Department of Systems Biology, Technical University of Denmark, DK 15.05 – 15.30 In-vivo like performance of microgravity spheroids in-vitro L7.2 Stephen J. Fey, University of Southern Denmark, DK 15.30 – 15.55 Towards Holistic Biotechnology – L7.3 An Industrial Perspective on Next Generation Cell Culture Processes Ali Kazemi Seresht Cell Culture Technology, Novo Nordisk A/S, DK 15.55 – 16.00 Closing remarks Lars Haastrup Pedersen, DBS, DK 7 Animal Cell Cultures - Expression and Engineering 8 Lecture abstracts 9 Animal Cell Cultures - Expression and Engineering 10 L1.1 The genome area of CHO: from blackbox optimisation to designer cell lines? Nicole Borth University of Natural Resources and Life Sciences Vienna (BOKU) and Austrian Center of Industrial Biotechnology (ACIB), Austria During the last 25 years, recombinant protein production in Chinese Hamster Ovary cells has reached a level of optimisation that has turned it into an “established technology”. Indeed, the improvements in yields achieved are remarkable: titers have increased from 0.1-0.4 g/l to reliable 2-6 g/l, with top values of 20 g/l reported. Nevertheless this achievement was based on empirical approaches and was largely due to enhancements of the medium composition and the higher cell densities thus reached. Details of what happened inside the cells have remained mostly a blackbox, despite the advances accomplished. With the availability of a genomic sequence, both of several CHO cell lines and the reference genome from the Chinese hamster, there now is the opportunity to accomplish a step change in our understanding and control of both the bioprocesses and cell behaviour. In parallel, new tools and methods are emerging that enable precise genome and epigenome editing, which together with the sequence information will enable the generation of designer cell lines, with precisely edited properties for different product categories. The present talk will give an overview over the available CHO databases and sequences and will then focus on how this genomic information can be used to obtain a comprehensive understanding of the regulatory network in place in this industrially used production cell line. In addition, the currently discussed plans for development of infrastructure and software to be available at www.CHOgenome.org will be discussed. 11 Animal Cell Cultures - Expression and Engineering L1.2 Microparticles as cell-to-cell communicators to empower therapies and technology: the case of megakaryocytic microparticles Eleftherios Terry Papoutsakis & Jinlin Jiang University of Delaware, USA A