TMC6 (EVER1) Antibody Affinity Purified Polyclonal Antibody Catalog # AG1387

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TMC6 (EVER1) Antibody Affinity purified polyclonal antibody Catalog # AG1387

Specification

TMC6 (EVER1) Antibody - Product Information

Application WB Primary Accession Q7Z403 Reactivity Human, Rat Host Rabbit Clonality Polyclonal Calculated MW 90045 Homology Rat, mouse - 13/14 amino acid residues are identical. Western blot analysis of rat prostate TMC6 (EVER1) Antibody - Additional (lanes 1and 4), rat testis (lanes 2 and 5) Information and rat placenta (lanes 3 and 6): 1-3. Anti-TMC6 (EVER1) antibody (#AG1387), Gene ID (1:800). 11322 4-6. Anti-TMC6 (EVER1) antibody, preincubated with the control peptide antigen. Other Names Transmembrane channel-like protein 6, Epidermodysplasia verruciformis protein 1, TMC6 (EVER1) Antibody - Background Protein LAK-4, TMC6, EVER1, EVIN1 EVER gene belongs to larger family of proteins Related products for control referred to as the transmembrane channel-like experiments 1 protein (TMC) family . EVER1/TMC6 protein, Control peptide antigen (supplied with the integral membrane protein, is located in the antibody free of charge). endoplasmic reticulum (ER) of keratinocytes, where it forms a complex with zinc transporter-1 Target/Specificity Peptide CGPFRTLDTMYEAGR, corresponding (ZnT-1) a membrane protein responsible for zinc efflux and resistance to zinc toxicity, thereby to amino acid residues 681-695 of human 2 TMC6 (Accession Q7Z403). Intracellular, controlling the cellular zinc balance . The EVER1 Cytoplasm. protein is predicted to have 10 transmembrane domains and 2 leucine zipper motifs1. The Dilution EVER1 gene encodes 2 alternatively spliced WB~~1:200-1:2000 proteins, one of 805 amino acids and the other of 454 amino acids, in the same open reading Peptide Confirmation frame. The EVER genes are thought to be Confirmed by mass-spectrography and amino transcribed in numerous tissues including the acid analysis. skin1. Mutations in the TMC6 are implicated in the development of the rare autosomal recessive Format disease Epidermodysplasia Verruciformis (EV)3. Affinity purified antibody, lyophilized powder EV is characterized by extreme susceptibility to infection by cutaneous human papillomaviruses Reconstitution (HPV) of the beta genus. The TMC6 genes may 25 µl, 50 µl or 0.2 ml deionized water, be involved in controlling HPV gene expression depending on the sample size. and replication in epidermal keratinocytes or they may directly affect innate and adaptive Antibody Concentration After immune responses which control the clearance of Reconstitution 4 HPV-infected keratinocytes in EV cases . A recent 0.85 mg/ml. report indicated an association between genetic variation in the TMC6 region and development of Storage Before Reconstitution cervical cancer (CxCa) 5. Lyophilized powder can be stored intact at room temperature for several weeks. For TMC6 (EVER1) Antibody - References longer periods, it should be stored at -20°C. References 1. Lazarczyk, M. et al. (2009) Storage After Reconstitution Microbiol. Mol. Biol. Rev. 73, 348. 2. Lazarczyk, The reconstituted solution can be stored at M. et al. (2008) J. Exp. Med. 205, 35. 3. Ramoz, 4ºC for up to 2 weeks. For longer periods, N. et al. (2002) Nat. Genet. 32, 579. 4. Orth, G. small aliquots should be stored at -20ºC or et al. (2008) Curr. Top Microbiol. Immunol. 321, below. Avoid multiple freezing and thawing. 59. 5. Castro, F.A. et al. (2012) Int. J. Cancer 130, The further dilutions should be made using a 349. carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control Antigen Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control Antigen Reconstitution 100 µl DDW.

Control Antigen Storage After Reconstitution -20ºC.

Preadsorption Control 1 µg peptide per 1 µg antibody.

Formulation Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

TMC6 (EVER1) Antibody - Protocols

Provided below are standard protocols that you may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture