Acylase I From porcine

Product Number A3010 Storage Temperature -20 °C

Product Description Aminoacylase from pig kidney is activated4 by EC Number: 3.5.1.14 Zn2+ and Co2+ and inhibited by SH blocking reagents, CAS Number: 9012-37-7 disulfide reducing reagents, N-tosyl-L-alanine, Molecular Weight: 86 kDa1 DL-norleucine, and N-tosyl-L-lysine Synonyms: N-Acylamino acid , chloromethylketone.5 Aminoacylase Precautions and Disclaimer Acylase I from porcine kidney is a dimer consisting of For Laboratory Use Only. Not for drug, household or 2 equal subunits of 43 kDa. Each subunit contains other uses. 12 sulfhydryl groups and 2 disulfide bonds. The also has a divalent (Zn2+) associated Preparation Instructions with it.1 This enzyme is soluble in potassium phosphate buffer, pH 7.0, (1 mg/ml), yielding a clear solution. Acylase from porcine kidney catalyzes the following reaction: References

N-acyl-L- + H2O®a carboxylate + L-amino 1. Hernandez, A., and Pina, E., A Simplified acid. Purification Method of Aminoacylase I. Prep. Biochem., 19, 247-249 (1989). The enzyme hydrolyzes a variety of N-acyl-L-amino 2. Enzyme Handbook, 1st ed., II, Barman, T. E., ed., acids and dehydropeptides containing dehydroalanyl Springer-Verlag (Berlin, Germany: 1969), p. 656. residues. N-acyl-D-amino acids and dehydropeptides 3. Henseling, J., and Rohm, K. R., Unusual Solvent other than dehydroalanine are not hydrolyzed. The Isotope Effects Of the Aminoacylase-catalyzed following compounds are hydrolyzed by acylase I: Hydrolysis Of Acetylamino Acids. FEBS Lett., 219, chloroacetyl-L-amino acids, L-leucinamide, 27-30 (1987). chloroacetyldehydroalanine, glycyl-L-amino acids, and 4. Greenstein, J.P., Dehydropeptidases from kidney. glycyldehydroalanine.2 Aminoacylase is widely used Meth. Enzymol., II, 109-119 (1955). as a reagent to resolve amino acid racemates. The Km 5. Enzyme Handbook, 4, Schomberg, D., and for acyl amino acids have been reported in the Salzmann, M., eds., Springer-Verlag (Berlin, literature: Ac-L-Met (0.99 mM), Ac-L-Glu (10.2 mM), Germany: 1991), EC 3.5.1.14, pp. 1-4 (1991). and Ac-L-Phe (5.5 mM).3 TMG/RXR 6/06

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