Ch 13 Game

review A cross between dissimilar individuals to bring together their best characteristics is called

A B Inbreeding C Hybridization D Sequencing • Ans: C Used to insert new genes into plant cells • A Gel electrophoresis • B • C Polymerase chain reaction • D Inbreeding Ans: B Makes many copies of a DNA sample • A Gel electrophoresis • B Plasmid • C Polymerase chain reaction • D Inbreeding • Ans: C Separates DNA fragments

• A Gel electrophoresis • B Plasmid • C Polymerase chain reaction • D Inbreeding Ans: A Continued breeding of individuals with similar characteristics • A Gel electrophoresis • B Plasmid • C Polymerase chain reaction • D Inbreeding • Ans: D

Which of the following can be used to produce organisms with desirable traits? • A Inbreeding • B Genetic engineering • C Inducing mutations • D All of the above • Ans: D

A member of a population of genetically identical cells produced from a single cell is a • A Clone • B Plasmid • C Mutant • D Sequence • Ans: A

Changing the DNA of an organism is called • A Genetic engineering • B Hybridization • C Selective breeding • D Inbreeding • Ans: A

DNA can be cut into shorter sequences by proteins known as • A Restriction • B • C Mutagens • D Clones • Ans: A

Which of the following characteristics does NOT apply to a plasmid? • A Made of DNA • B In animal cells • C Circular • D Accepts foreign DNA • Ans: B What has been produced in the drawing below? • A A clone • B Recombinant DNA • C A • D A • Ans: B

When cell transformation is successful, the recombinant DNA • A Undergoes mutation • B Is treated with antibiotics • C Becomes part of the transformed cell’s genome • D Becomes a nucleus • Ans: C Bacteria often contain small circular molecules of DNA known as • A Clones • B Restriction enzymes • C Plasmids • D Hybrids • Ans: C

Organisms that contain genes from other organisms are called • A Transgenic • B Mutagenic • C Donor organisms • D Cloned organisms • Ans: A Which of the following is most likely to bring together two recessive alleles for a genetic defect? • A hybridization • B inbreeding • C genetic engineering • D transformation • Ans: B Scientists produced oil-eating bacteria by • A inbreeding bacteria • B making bacteria polyploid • C hybridizing bacteria • D inducing mutations in bacteria • Ans: D Which of the following includes all of the others? • A inbreeding • B hybridization • C induced mutations • D selective breeding • Ans: D Polyploidy instantly results in a new plant species because it • A changes a species’ number of chromosomes • B causes mutations • C produces a hardier species • D all of the above • Ans: A Knowing the sequence of an organism’s DNA allows researchers to • A reproduce the organism • B study specific genes • C cut the DNA • D mutate the DNA • Ans: B

Suppose a restriction recognizes the six- base sequence GTTAAC CAATTG in a double strand of DNA. If the cut creates two sticky ends that are four bases long, what will one of the exposed sequences (sticky ends) be? • A GTTA • B CAAT • C TTAA • D AATG • Ans: C

Which of the following includes all of the others inside of it? • A transformed bacterium • B foreign gene • C recombinant DNA • D plasmid • Ans: A

To produce transgenic bacteria that make , which of the following steps did scientists have to take first? • A Insert the human insulin gene into a plasmid • B Extract the insulin from the bacterial culture • C Use a to cut out the insulin gene from human DNA • D Transform bacteria with the recombinant plasmid • Ans: C

Which of the following is a clone? (TRIPLE PTS) • A the adult female sheep whose DNA was used to produce Dolly • B a transgenic mouse • C a bacterium taken from a bacterial colony • D the plant with the luciferase gene • Ans: C

Use p. 339, #3; What can the researcher conclude about the bacteria that grow on the nutrient medium? • A They are resistant to ampicillan. • B They contain recombinant DNA • C They contain a human gene • D All of the above • Ans: D

Use p. 339, #4; Which of the following would indicate that the bacteria contain the human gene?

• A They produce the human protein encoded by the human gene. • B They produce ampicillin. • C Both A and B • D Neither A nor B • Ans: A Use p. 339, #9; What can you conclude about cycles 18-26? • A PCR produced accurate copies of template DNA at an exponential rate. • B The amount of DNA produced by PCR doubled with each cycle of the reaction. • C The DNA copies produced by PCR were not accurate copies of the original DNA template. • D A and B only. • Ans: D Use p. 339, #10; Based on the graph, which of the following might have happened between cycles 26 and 28? • A PCR stopped producing accurate copies of the template. • B The rate of the reaction slowed down. • C All the template DNA was used up. • D A and C only. • Ans: B