Brian O'neal Bachmann, Ph.D
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Prepared: January 5, 2011 BRIAN O’NEAL BACHMANN, PH.D. Departments of Chemistry and Biochemistry Vanderbilt University Nashville, TN 37235-1822 Phone: (615) 322-8865; Fax: (615) 343-1234 E-mail: [email protected] http://www.vanderbilt.edu/AnS/Chemistry/BachmannGroup/ DEGREES EARNED 1995 – 2000 The Johns Hopkins University, Baltimore, Maryland. Ph.D. degree in Chemistry, December, 2000. Dissertation entitled: Identification and Characterization of β−Lactam Synthetase of Clavulanic Acid Biosynthesis. Advisor: Professor Craig. A. Townsend 1993 – 1994 Southern Methodist University, Dallas, Texas. M.S. degree in Chemistry, December 1994. Thesis entitled: Synthesis and Evaluation of Mechanism Based β−lactamase Inhibitors. Advisor: Professor John D. Buynak 1988 – 1992 Virginia Tech, Blacksburg, Virginia. B.S. degree in Chemistry, December 1992. Undergraduate Research Advisor: Tomas Hudlicky EMPLOYMENT HISTORY 2000 – 2001 Postdoctoral Fellow, The Johns Hopkins University Department of Chemistry, Baltimore, Maryland 2001 – 2002 Assistant Director of Chemistry, Ecopia Biosciences (now Thallion Pharmaceuticals), Montreal, Quebec, Canada 2002 – 2003 Director of Chemistry, Ecopia Biosciences (now Thallion Pharmaceuticals), Montreal, Quebec, Canada 2003 – current Assistant Professor of Chemistry, Vanderbilt University Department of Chemistry, Nashville, Tennessee 2000 – current Director, Board of Directors, Dymax Corporation, Torrington, Connecticut HONORS AND AWARDS • American Society of Chemists: Outstanding Graduate Student in Chemistry, 1994 • Commercial Development Award – Dymax Corporation, 1995 • Sonneborn Graduate Fellowship, 1997 – 1999 • Alexander Kossiakoff Graduate Student, 2000 • Sarah and Adolph Roseman Achievement Award in for Outstanding Accomplishment in Chemistry, 2000 • Petroleum Research Fund type G award, 2004 1 • RO1 ‘model proposal’ at NIH workshop for new investigators, 2010 PROFESSIONAL SOCIETIES AND ACTIVITIES • American Chemical Society (member) 1995 - present • Society for Industrial Microbiology (member) 2000 - present • American Society of Microbiology (member) 2010 - present • National Association of Corporate Directors (member) 2001- present • Reviewer for Nature Chemical Biology, Proceedings of the National Academy of Sciences, Journal of the American Chemical Society, Chemistry & Biology, Journal of Biological Chemistry and Applied Environmental Microbiology among others • Reviewer for the Petroleum Research Fun Type G- Grant award, 2004 • Study Section Member for Program Project Award, National Inst. Health, 2005 • Study Section Member (Ad Hoc) for SBC-B, Nat. Inst. Health 2006, 2010 • Session Convener, Society for Industrial Microbiology Annual Meeting, 2006 RESEARCH Published Articles (Corresponding author underlined) Independent Work 1. Panosian, T. D., Nannemann, D. P., Watkins, G., Phelan, V. V., McDonald, W. H., Wadzinski, B. E., Bachmann, B. O. and Iverson, T. M., The Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle. Journal of Biological Chemistry. 2010, . (in press) Pubmed. DOI. 2. Vey, J. L., Al-Mestarihi, A., Hu, Y., Funk, M. A., Bachmann, B. O. and Iverson, T. M., Structure and mechanism of ORF36, an amino sugar oxidizing enzyme in everninomicin biosynthesis. Biochemistry. 2010, 49, 9306-17.Pubmed. DOI. 3. Akif, M., Ntai, I., Sturrock, E. D., Isaac, R. E., Bachmann, B. O. and Acharya, K. R., Crystal structure of a phosphonotripeptide K-26 in complex with angiotensin converting enzyme homologue (AnCE) from Drosophila melanogaster. Biochemical and biophysical research communications. 2010, . (in press). Pubmed. DOI. 4. Bachmann, B. O., McNees, R., Melancon, B. J., Ghidu, V. P., Clark, R., Crews, B. C., Deguire, S. M., Marnett, L. J. and Sulikowski, G. A., Light-induced isomerization of apoptolidin A leads to inversion of C2-C3 double bond geometry. Organic Letters. 2010, 12, 2944-2947. Pubmed. DOI. 2 5. Panosian, T. D., Nannemann, D. P., Bachmann, B. O. and Iverson, T. M., Crystallization and preliminary X-ray analysis of a phosphopentomutase from Bacillus cereus. Acta Crystallographica F. 2010, 66, 811-814. Pubmed. DOI. 6. Du, Y., Gisselberg, J. E., Johnson, J. D., Lee, P. J., Prigge, S. T. and Bachmann, B. O., Lactococcus lactis fabH, encoding β−ketoacyl-acyl carrier protein synthase, can be functionally replaced by the Plasmodium falciparum congener. Applied and Environmental Microbiology. 2010, 76, 3959-3966. Pubmed. DOI. 7. Nannemann, D. P., Kaufmann, K. W., Meiler, J. and Bachmann, B. O., Design and directed evolution of a dideoxy purine nucleoside phosphorylase. Protein Engineering Design and Selection. 2010, 23, 607-616. Pubmed. DOI. 8. Reniere, M. L., Ukpabi, G. N., Harry, S. R., Stec, D. F., Krull, R., Wright, D. W., Bachmann, B. O., Murphy, M. E. and Skaar, E. P., The IsdG-family of haem oxygenases degrades haem to a novel chromophore. Molecular Microbiology. 2010, 75, 1529-1538. Pubmed. DOI. 9. Bachmann, B. O., Biosynthesis: is it time to go retro? (commentary). Nature Chemical Biology. 2010, 6, 390-393. Pubmed. DOI. 10. Carter, M. D., Phelan, V. V., Sandlin, R. D., Bachmann, B. O. and Wright, D. W., Lipophilic mediated assays for β−hematin inhibitors. Combinatorial Chemistry & High Throughput Screening. 2010, 13, 285-292. Pubmed. 11. Scism, R. A. and Bachmann, B. O., Five-component cascade synthesis of nucleotide analogues in an engineered self-immobilized enzyme aggregate. Chembiochem. 2010, 11, 67-70. Pubmed. DOI. 12. Phelan, V. V., Du, Y., McLean, J. A. and Bachmann, B. O., Adenylation enzyme 18 characterization using γ − O4-ATP pyrophosphate exchange. Chemistry & Biology. 2009, 16, 473-478. Pubmed. DOI. 13. Ghidu, V. P., Ntai, I., Wang, J., Jacobs, A. T., Marnett, L. J., Bachmann, B. O. and Sulikowski, G. A., Combined chemical and biosynthetic route to access a new apoptolidin congener. Organic Letters. 2009, 11, 3032-3034. Pubmed. DOI. 14. Ntai, I. and Bachmann, B. O., Identification of ACE pharmacophore in the phosphonopeptide metabolite K-26. Bioorganic & Medicinal Chemistry Letters. 2008, 18, 3068-3071. Pubmed. DOI. 15. Hu, Y., Phelan, V. V., Farnet, C. M., Zazopoulos, E. and Bachmann, B. O., Reassembly of anthramycin biosynthetic gene cluster by using recombinogenic cassettes. Chembiochem. 2008, 9, 1603-1608. Pubmed. DOI. 16. Hu, Y., Al-Mestarihi, A., Grimes, C. L., Kahne, D. and Bachmann, B. O., A unifying nitrososynthase involved in nitrosugar biosynthesis. Journal of the American Chemical Society. 2008, 130, 15756-15757. Pubmed. DOI. 3 17. Scism, R. A., Stec, D. F. and Bachmann, B. O., Synthesis of nucleotide analogues by a promiscuous phosphoribosyltransferase. Organic Letters. 2007, 9, 4179-4182. Pubmed. DOI. 18. Hu, Y., Phelan, V., Ntai, I., Farnet, C. M., Zazopoulos, E. and Bachmann, B. O., Benzodiazepine biosynthesis in Streptomyces refuineus. Chemistry and Biology. 2007, 14, 691- 701. Pubmed. DOI. 19. Bachmann, B. O., Foundations for directed alkaloid biosynthesis (commentary). Chemistry and Biology. 2007, 14, 875-876. Pubmed. DOI. 20. Wilkinson, B. and Bachmann, B. O., Biocatalysis in pharmaceutical preparation and alteration. Current Opinion in Chemical Biology. 2006, 10, 169-176. Pubmed. DOI. 21. Sulikowski, M. M. and Bachmann, B. O., Designing a curriculum that goes beyond a list of topics (commentary). ACS Chemical Biology. 2006, 1, 489-491. Pubmed. DOI. 22. Ntai, I., Phelan, V. V. and Bachmann, B. O., Phosphonopeptide K-26 biosynthetic intermediates in Astrosporangium hypotensionis. Chemical Communications. 2006, 4518-4520. Pubmed. DOI. 23. Ntai, I., Manier, M. L., Hachey, D. L. and Bachmann, B. O., Biosynthetic origins of C-P bond containing tripeptide K-26. Organic Letters. 2005, 7, 2763-2765. Pubmed. DOI. 24. Bachmann, B. O., Decoding chemical structures from genomes (commentary). Nature Chemical Biology. 2005, 1, 244-245. (Commentary). Pubmed. DOI. Book Chapters 25. Bachmann, B. O. and Ravel, J., Chapter 8. Methods for in silico prediction of microbial polyketide and nonribosomal peptide biosynthetic pathways from DNA sequence data. Methods in enzymology. 2009, 458, 181-217. Pubmed. DOI. Submitted Articles (review pending 2010) 26. Timothy D. Panosian, David P. Nannemann, Guy Watkins, Brian Wadzinski, Brian O. Bachmann, Tina M. Iverson. Turnover of Bacillus cereus phosphopentomutase is primed by phosphorylation of Thr-85 and triggered by ribose-5-phosphate binding Journal of Biological Chemistry, submitted June 2010. 27. Jessica L. Vey, Ahmad Al-Mestarihi, Yunfeng Hu, Michael A. Funk, Brian O. Bachmann, and T. M. Iverson. Mechanism of amino sugar oxidation during everninomycin biosynthesis. Journal of the American Chemical Society, submitted June 2010. 4 28. Larissa Spell Fen, Cody Goodwin, Brian O. Bachman and John McLean. Ion mobility mass spectrometry for peptide natural product discovery. Journal of the American Chemical Society 2003, and prior to Vanderbilt 29. McAlpine, J. B., Bachmann, B. O., Piraee, M., Tremblay, S., Alarco, A. M., Zazopoulos, E. and Farnet, C. M., Microbial Genomics as a guide to drug discovery and structural elucidation: ECO-02301, a novel antifungal agent, as an example. Journal of Natural Products. 2005, 68, 493-496. DOI. 30. Zazopoulos, E., Huang, K. X., Staffa, A., Liu, W., Bachmann, B. O., Nonaka, K., Ahlert, J., Thorson, J. S., Shen, B. and Farnet, C. M., A genomics-guided approach for discovering and expressing cryptic metabolic pathways. Nature Biotechnology. 2003, 21, 187-190. DOI. 31. Miller, M. T., Bachmann, B.