DOCSLIB.ORG

Nodaviridae Phenuiviridae Dicistroviridae Permutotetraviridae

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Nodaviridae Phenuiviridae Dicistroviridae Permutotetraviridae Nodaviridae Phenuiviridae Uukuniemi virus AIU95037.1 Nursery bend carp nodavirus 100 85 Edward river carp nodavirus 99 Zaliv Terpeniya virus QCF29627.1 97Wemen carp nodavirus 100 Chize virus AFH08728.1 94 77 Grand Arbaud virus AFH08732.1 86Murray-Darling rainbowfish nodavirus 100 Murre virus AFH08736.1 89 Macrobrachium rosenbergii nodavirus AAQ54758.2 100 Precarious point virus AEL29680.1 100 Macquarie river carp nodavirus 91 Manawa virus AFN73042.1 100 Barwon river carp nodavirus Kabuto mountain virus YP_009449450.1 91 85 Penaeus vannamei nodavirus YP_004207810.1 Huangpi Tick Virus 2 YP_009293590.1 100Khasan virus AII79370.1 Nodamura virus NP_077730.1 100 60 Kaisodi virus YP_009551639.1 100 Black beetle virus YP_053043.1 72 99Flock House virus NP_689444.1 Silverwater virus AIU95032.1 59 Bole Tick Virus 1 AJG39234.1 100 Eastern mosquitofish nodavirus 53 FTLS virus AID69020.1 Boolarra virus NP_689439.1 100 100 Severe fever with thrombocytopenia syndrome virus QPF77321.1 Betanodavirus sp. AVM87352.1 58 Heartland virus YP_009047242.1 99 43 Golden pompano nervous necrosis virus AEK48148.1 36 Pena Blanca virus QBQ01759.1 90 92 Senegalese sole Iberian betanodavirus YP_009047239.1 100 Anhanga virus YP_009346019.1 Redspotted grouper nervous necrosis virus YP_611155.1 Urucuri virus YP_009346026.1 96 90 Atlantic cod betanodavirus Ac06NorPmB ABU95420.1 Blacklegged tick phlebovirus 1 ANT80544.1 99 100 Sara tick phlebovirus QPD01621.1 10099 Barfin flounder virus BF93Hok ACF36168.1 100 Tiger puffer nervous necrosis virus YP_003288759.1 Murray-Darling rainbowfish phenuivirus Hubei diptera virus 3 YP_009329894.1 Striped jack nervous necrosis virus NP_599247.1 100 100 Lunovirus AKA58518.1 Parry’s Creek phasivirus 1 QLJ83473.1 97 Gouleako virus ABP68557.1 Pariacoto virus NP_620109.1 100 94 Yichang Insect virus YP_009305143.1 100 78 Alphanodavirus HB 2007/CHN ADF97523.1 Mantodean phenui related virus OKIAV283 QMP82145.1 Bat guano associated nodavirus GF 4n ADI48250.1 100 Hemipteran phenui related virus OKIAV285 QMP82212.1 0.4 0.3 Dicistroviridae Permutotetraviridae Drosophila C virus AWI97853.1 100 Culex Daeseongdong-like virus AXQ04818.1 97 Warroolaba Creek virus 2 QIJ25855.1 100 Lake Burrendong carp dicsitrovirus Daeseongdong virus 2 YP_009182196.1 93 100 Cricket paralysis virus AKA63263.1 100 97 Drosophila A virus AKH67341.1 Nilaparvata lugens C virus AIY53985.1 28 Anopheles C virus YP_009252204.1 Euprosterna elaeasa virus NP_573541.1 99 100 Bundaberg bee AWK77856 22 59 Thosea asigna virus YP_009665207.1 100 Goose dicistrovirus QIJ70020.1 Drosophila kikkawai virus 1 AYQ66681.1 52 Niehaus virus AOC55066.1 49 Israeli acute paralysis virus ASS83234.1 100 Sanxia water strider virus 19 YP_009337667.1 100 Kashmir bee virus QIN93564.1 Shinobi tetravirus YP_009553485.1 100 Linepithema humile virus 1 AWK91473.1 92 Atrpec virus 1 AYN75539.1 100 100 Culex permutotetra-like virus BBO25553.1 Wenzhou channeled applesnail virus 2 YP_009336777.1 100 Halhan virus AYN75548 Newfield virus AWY11097.1 88 Centovirus AC YP_009315868.1 100 Hubei permutotetra-like virus 2 YP_009337336.1 Midge dicistrovirus AOX47515.1 100 100 Fletcher virus AOX15248.1 91 Sanxia permutotetra-like virus 1 YP_009337650.1 100 72 Mosquito dicistrovirus YP_009315870.1 Murray-Darling carp permutotetravirus 100 Edward river carp dicistrovirus 100 Robinvale bee virus 3 AWK77878.1 Egaro virus YP_009272815.1 0.3 0.4.
Load more

Recommended publications
  • Differential Segregation of Nodaviral Coat Protein and RNA Into Progeny Virions During Mixed Infection with FHV and Nov
    Virology 454-455 (2014) 280–290 Contents lists available at ScienceDirect Virology journal homepage: www.elsevier.com/locate/yviro Differential segregation of nodaviral coat protein and RNA into progeny virions during mixed infection with FHV and NoV Radhika Gopal, P. Arno Venter 1, Anette Schneemann n Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA article info abstract Article history: Nodaviruses are icosahedral viruses with a bipartite, positive-sense RNA genome. The two RNAs are Received 30 December 2013 packaged into a single virion by a poorly understood mechanism. We chose two distantly related Returned to author for revisions nodaviruses, Flock House virus and Nodamura virus, to explore formation of viral reassortants as a 27 January 2014 means to further understand genome recognition and encapsidation. In mixed infections, the viruses Accepted 3 March 2014 were incompatible at the level of RNA replication and their coat proteins segregated into separate Available online 21 March 2014 populations of progeny particles. RNA packaging, on the other hand, was indiscriminate as all four viral Keywords: RNAs were detectable in each progeny population. Consistent with the trans-encapsidation phenotype, Flock House virus fluorescence in situ hybridization of viral RNA revealed that the genomes of the two viruses co-localized Nodamura virus throughout the cytoplasm. Our results imply that nodaviral RNAs lack rigorously defined packaging Mixed infection signals and that co-encapsidation of the viral RNAs does not require a pair of cognate RNA1 and RNA2. Viral assembly & RNA encapsidation 2014 Elsevier Inc. All rights reserved. Viral reassortant Introduction invaginations of the outer membrane of the organelle (Kopek et al., 2007).
    [Show full text]
  • Bulged Stem-Loop
    Proc. Nati. Acad. Sci. USA Vol. 89, pp. 11146-11150, December 1992 Biochemisty Evidence that the packaging signal for nodaviral RNA2 is a bulged stem-loop (defecfive-interfering RNA/blpartite genone/RNA p ag) WEIDONG ZHONG, RANJIT DASGUPTA, AND ROLAND RUECKERT* Institute for Molecular Virology and Department of Biochemistry, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706 Communicated by Paul Kaesberg, August 26, 1992 ABSTRACT Flock house virus is an insect virus ging tion initiation site of T3 promoter and the cloned DI DNA to the family Nodaviridae; members of this family are char- through oligonucleotide-directed mutagenesis. Such RNA acterized by a small bipartite positive-stranded RNA genome. transcripts, however, still had four extra nonviral bases atthe The blrger genomic m , RNA1, encodes viral repliation 3' end (4). proteins, whereas the smaller one, RNA2, e coat protein. In Viro Transcription of Cloned FIV DNA. Selected DNA Both RNAsarepa ed in a single particle. A defective- clones were cleaved with the restriction enzyme Xba I, and interferin RNA (DI-634), isolated from a line of DrosophUa the resulting linear DNA templates (0.03 mg/ml) were tran- cells persistently infected with Flock house virus, was used to scribed with T3 RNA polymerase as described by Konarska show that a 32-base regionofRNA2 (bases 186-217) is required et al. (20). One-half millimolar guanosine(5')triphospho- for pcaing into virions. RNA folding analysis predicted that (5')guanosine [G(5')ppp(5')GI was included in the reaction this region forms a stem-loop structure with a 5-base loop and mixture to provide capped transcripts.
    [Show full text]
  • A Preliminary Study of Viral Metagenomics of French Bat Species in Contact with Humans: Identification of New Mammalian Viruses
    A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses. Laurent Dacheux, Minerva Cervantes-Gonzalez, Ghislaine Guigon, Jean-Michel Thiberge, Mathias Vandenbogaert, Corinne Maufrais, Valérie Caro, Hervé Bourhy To cite this version: Laurent Dacheux, Minerva Cervantes-Gonzalez, Ghislaine Guigon, Jean-Michel Thiberge, Mathias Vandenbogaert, et al.. A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.. PLoS ONE, Public Library of Science, 2014, 9 (1), pp.e87194. 10.1371/journal.pone.0087194.s006. pasteur-01430485 HAL Id: pasteur-01430485 https://hal-pasteur.archives-ouvertes.fr/pasteur-01430485 Submitted on 9 Jan 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License A Preliminary Study of Viral Metagenomics of French Bat Species in Contact with Humans: Identification of New Mammalian Viruses Laurent Dacheux1*, Minerva Cervantes-Gonzalez1,
    [Show full text]
  • Virus Particle Structures
    Virus Particle Structures Virus Particle Structures Palmenberg, A.C. and Sgro, J.-Y. COLOR PLATE LEGENDS These color plates depict the relative sizes and comparative virion structures of multiple types of viruses. The renderings are based on data from published atomic coordinates as determined by X-ray crystallography. The international online repository for 3D coordinates is the Protein Databank (www.rcsb.org/pdb/), maintained by the Research Collaboratory for Structural Bioinformatics (RCSB). The VIPER web site (mmtsb.scripps.edu/viper), maintains a parallel collection of PDB coordinates for icosahedral viruses and additionally offers a version of each data file permuted into the same relative 3D orientation (Reddy, V., Natarajan, P., Okerberg, B., Li, K., Damodaran, K., Morton, R., Brooks, C. and Johnson, J. (2001). J. Virol., 75, 11943-11947). VIPER also contains an excellent repository of instructional materials pertaining to icosahedral symmetry and viral structures. All images presented here, except for the filamentous viruses, used the standard VIPER orientation along the icosahedral 2-fold axis. With the exception of Plate 3 as described below, these images were generated from their atomic coordinates using a novel radial depth-cue colorization technique and the program Rasmol (Sayle, R.A., Milner-White, E.J. (1995). RASMOL: biomolecular graphics for all. Trends Biochem Sci., 20, 374-376). First, the Temperature Factor column for every atom in a PDB coordinate file was edited to record a measure of the radial distance from the virion center. The files were rendered using the Rasmol spacefill menu, with specular and shadow options according to the Van de Waals radius of each atom.
    [Show full text]
  • Data Mining Cdnas Reveals Three New Single Stranded RNA Viruses in Nasonia (Hymenoptera: Pteromalidae)
    Insect Molecular Biology Insect Molecular Biology (2010), 19 (Suppl. 1), 99–107 doi: 10.1111/j.1365-2583.2009.00934.x Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae) D. C. S. G. Oliveira*, W. B. Hunter†, J. Ng*, Small viruses with a positive-sense single-stranded C. A. Desjardins*, P. M. Dang‡ and J. H. Werren* RNA (ssRNA) genome, and no DNA stage, are known as *Department of Biology, University of Rochester, picornaviruses (infecting vertebrates) or picorna-like Rochester, NY, USA; †United States Department of viruses (infecting non-vertebrates). Recently, the order Agriculture, Agricultural Research Service, US Picornavirales was formally characterized to include most, Horticultural Research Laboratory, Fort Pierce, FL, USA; but not all, ssRNA viruses (Le Gall et al., 2008). Among and ‡United States Department of Agriculture, other typical characteristics – e.g. a small icosahedral Agricultural Research Service, NPRU, Dawson, GA, capsid with a pseudo-T = 3 symmetry and a 7–12 kb USA genome made of one or two RNA segments – the Picor- navirales genome encodes a polyprotein with a replication Abstractimb_934 99..108 module that includes a helicase, a protease, and an RNA- dependent RNA polymerase (RdRp), in this order (see Le We report three novel small RNA viruses uncovered Gall et al., 2008 for details). Pathogenicity of the infections from cDNA libraries from parasitoid wasps in the can vary broadly from devastating epidemics to appar- genus Nasonia. The genome of this kind of virus ently persistent commensal infections. Several human Ј is a positive-sense single-stranded RNA with a 3 diseases, from hepatitis A to the common cold (e.g.
    [Show full text]
  • Epidemiology of White Spot Syndrome Virus in the Daggerblade Grass Shrimp (Palaemonetes Pugio) and the Gulf Sand Fiddler Crab (Uca Panacea)
    The University of Southern Mississippi The Aquila Digital Community Dissertations Fall 12-2016 Epidemiology of White Spot Syndrome Virus in the Daggerblade Grass Shrimp (Palaemonetes pugio) and the Gulf Sand Fiddler Crab (Uca panacea) Muhammad University of Southern Mississippi Follow this and additional works at: https://aquila.usm.edu/dissertations Part of the Animal Diseases Commons, Disease Modeling Commons, Epidemiology Commons, Virology Commons, and the Virus Diseases Commons Recommended Citation Muhammad, "Epidemiology of White Spot Syndrome Virus in the Daggerblade Grass Shrimp (Palaemonetes pugio) and the Gulf Sand Fiddler Crab (Uca panacea)" (2016). Dissertations. 895. https://aquila.usm.edu/dissertations/895 This Dissertation is brought to you for free and open access by The Aquila Digital Community. It has been accepted for inclusion in Dissertations by an authorized administrator of The Aquila Digital Community. For more information, please contact [email protected]. EPIDEMIOLOGY OF WHITE SPOT SYNDROME VIRUS IN THE DAGGERBLADE GRASS SHRIMP (PALAEMONETES PUGIO) AND THE GULF SAND FIDDLER CRAB (UCA PANACEA) by Muhammad A Dissertation Submitted to the Graduate School and the School of Ocean Science and Technology at The University of Southern Mississippi in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy Approved: ________________________________________________ Dr. Jeffrey M. Lotz, Committee Chair Professor, Ocean Science and Technology ________________________________________________ Dr. Darrell J. Grimes, Committee Member Professor, Ocean Science and Technology ________________________________________________ Dr. Wei Wu, Committee Member Associate Professor, Ocean Science and Technology ________________________________________________ Dr. Reginald B. Blaylock, Committee Member Associate Research Professor, Ocean Science and Technology ________________________________________________ Dr. Karen S. Coats Dean of the Graduate School December 2016 COPYRIGHT BY Muhammad* 2016 Published by the Graduate School *U.S.
    [Show full text]
  • Emerging Viral Diseases of Fish and Shrimp Peter J
    Emerging viral diseases of fish and shrimp Peter J. Walker, James R. Winton To cite this version: Peter J. Walker, James R. Winton. Emerging viral diseases of fish and shrimp. Veterinary Research, BioMed Central, 2010, 41 (6), 10.1051/vetres/2010022. hal-00903183 HAL Id: hal-00903183 https://hal.archives-ouvertes.fr/hal-00903183 Submitted on 1 Jan 2010 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Vet. Res. (2010) 41:51 www.vetres.org DOI: 10.1051/vetres/2010022 Ó INRA, EDP Sciences, 2010 Review article Emerging viral diseases of fish and shrimp 1 2 Peter J. WALKER *, James R. WINTON 1 CSIRO Livestock Industries, Australian Animal Health Laboratory (AAHL), 5 Portarlington Road, Geelong, Victoria, Australia 2 USGS Western Fisheries Research Center, 6505 NE 65th Street, Seattle, Washington, USA (Received 7 December 2009; accepted 19 April 2010) Abstract – The rise of aquaculture has been one of the most profound changes in global food production of the past 100 years. Driven by population growth, rising demand for seafood and a levelling of production from capture fisheries, the practice of farming aquatic animals has expanded rapidly to become a major global industry.
    [Show full text]
  • Energetics of Quasiequivalence: Computational Analysis of Protein-Protein Interactions in Icosahedral Viruses
    546 Biophysical Journal Volume 74 January 1998 546–558 Energetics of Quasiequivalence: Computational Analysis of Protein-Protein Interactions in Icosahedral Viruses Vijay S. Reddy,* Heidi A. Giesing,* Ryan T. Morton,* Abhinav Kumar,* Carol Beth Post,# Charles L. Brooks, III,* and John E. Johnson* *Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, and #Department of Medicinal Chemistry, Purdue University, West Lafayette, Indiana 47907 USA ABSTRACT Quaternary structure polymorphism found in quasiequivalent virus capsids provides a static framework for studying the dynamics of protein interactions. The same protein subunits are found in different structural environments within these particles, and in some cases, the molecular switching required for the polymorphic quaternary interactions is obvious from high-resolution crystallographic studies. Employing atomic resolution structures, molecular mechanics, and continuum electrostatic methods, we have computed association energies for unique subunit interfaces of three icosahedral viruses, black beetle virus, southern bean virus, and human rhinovirus 14. To quantify the chemical determinants of quasiequivalence, the energetic contributions of individual residues forming quasiequivalent interfaces were calculated and compared. The potential significance of the differences in stabilities at quasiequivalent interfaces was then explored with the combinatorial assembly approach. The analysis shows that the unique association energies computed for each virus
    [Show full text]
  • Novel RNA Viruses from the Transcriptome of Pheromone Glands in the Pink Bollworm Moth, Pectinophora Gossypiella
    Entomology Publications Entomology 6-15-2021 Novel RNA Viruses from the Transcriptome of Pheromone Glands in the Pink Bollworm Moth, Pectinophora gossypiella Xiaoyi Dou Iowa State University Sijun Liu Iowa State University Victoria Soroker Institute of Plant Protection, ARO Ally Harari Institute of Plant Protection, ARO Russell Jurenka Iowa State University, [email protected] Follow this and additional works at: https://lib.dr.iastate.edu/ent_pubs Part of the Bioinformatics Commons, Entomology Commons, and the Genetics Commons The complete bibliographic information for this item can be found at https://lib.dr.iastate.edu/ ent_pubs/596. For information on how to cite this item, please visit http://lib.dr.iastate.edu/ howtocite.html. This Article is brought to you for free and open access by the Entomology at Iowa State University Digital Repository. It has been accepted for inclusion in Entomology Publications by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Novel RNA Viruses from the Transcriptome of Pheromone Glands in the Pink Bollworm Moth, Pectinophora gossypiella Abstract In this study, we analyzed the transcriptome obtained from the pheromone gland isolated from two Israeli populations of the pink bollworm Pectinophora gossypiella to identify viral sequences. The lab population and the field samples carried the same viral sequences. We discovered four novel viruses: two positive- sense single-stranded RNA viruses, Pectinophora gossypiella virus 1 (PecgV1, a virus of Iflaviridae) and Pectinophora gossypiella virus 4 (PecgV4, unclassified), and two negative-sense single-stranded RNA viruses, Pectinophora gossypiella virus 2 (PecgV2, a virus of Phasmaviridae) and Pectinophora gossypiella virus 3 (PecgV3, a virus of Phenuiviridae).
    [Show full text]
  • Diversity and Evolution of Viral Pathogen Community in Cave Nectar Bats (Eonycteris Spelaea)
    viruses Article Diversity and Evolution of Viral Pathogen Community in Cave Nectar Bats (Eonycteris spelaea) Ian H Mendenhall 1,* , Dolyce Low Hong Wen 1,2, Jayanthi Jayakumar 1, Vithiagaran Gunalan 3, Linfa Wang 1 , Sebastian Mauer-Stroh 3,4 , Yvonne C.F. Su 1 and Gavin J.D. Smith 1,5,6 1 Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore 169857, Singapore; [email protected] (D.L.H.W.); [email protected] (J.J.); [email protected] (L.W.); [email protected] (Y.C.F.S.) [email protected] (G.J.D.S.) 2 NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 119077, Singapore 3 Bioinformatics Institute, Agency for Science, Technology and Research, Singapore 138671, Singapore; [email protected] (V.G.); [email protected] (S.M.-S.) 4 Department of Biological Sciences, National University of Singapore, Singapore 117558, Singapore 5 SingHealth Duke-NUS Global Health Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore 168753, Singapore 6 Duke Global Health Institute, Duke University, Durham, NC 27710, USA * Correspondence: [email protected] Received: 30 January 2019; Accepted: 7 March 2019; Published: 12 March 2019 Abstract: Bats are unique mammals, exhibit distinctive life history traits and have unique immunological approaches to suppression of viral diseases upon infection. High-throughput next-generation sequencing has been used in characterizing the virome of different bat species. The cave nectar bat, Eonycteris spelaea, has a broad geographical range across Southeast Asia, India and southern China, however, little is known about their involvement in virus transmission.
    [Show full text]
  • Viral Nanoparticles and Virus-Like Particles: Platforms for Contemporary Vaccine Design Emily M
    Advanced Review Viral nanoparticles and virus-like particles: platforms for contemporary vaccine design Emily M. Plummer1,2 and Marianne Manchester2∗ Current vaccines that provide protection against infectious diseases have primarily relied on attenuated or inactivated pathogens. Virus-like particles (VLPs), comprised of capsid proteins that can initiate an immune response but do not include the genetic material required for replication, promote immunogenicity and have been developed and approved as vaccines in some cases. In addition, many of these VLPs can be used as molecular platforms for genetic fusion or chemical attachment of heterologous antigenic epitopes. This approach has been shown to provide protective immunity against the foreign epitopes in many cases. A variety of VLPs and virus-based nanoparticles are being developed for use as vaccines and epitope platforms. These particles have the potential to increase efficacy of current vaccines as well as treat diseases for which no effective vaccines are available. 2010 John Wiley & Sons, Inc. WIREs Nanomed Nanobiotechnol 2011 3 174–196 DOI: 10.1002/wnan.119 INTRODUCTION are normally associated with virus infection. PAMPs can be recognized by Toll-like receptors (TLRs) and he goal of vaccination is to initiate a strong other pattern-recognition receptors (PRRs) which Timmune response that leads to the development are present on the surface of host cells.4 The of lasting and protective immunity. Vaccines against intrinsic properties of multivalent display and highly pathogens are the most common, but approaches to ordered structure present in many pathogens also develop vaccines against cancer cells, host proteins, or 1,2 facilitate recognition by PAMPs, resulting in increased small molecule drugs have been developed as well.
    [Show full text]
  • Synthesis of Potato Virus X Rnas by Membrane- Containing Extracts
    JOURNAL OF VIROLOGY, July 1996, p. 4795–4799 Vol. 70, No. 7 0022-538X/96/$04.0010 Copyright q 1996, American Society for Microbiology Synthesis of Potato Virus X RNAs by Membrane- Containing Extracts SERGEY V. DORONIN AND CYNTHIA HEMENWAY* Department of Biochemistry, North Carolina State University, Raleigh, North Carolina 27695-7622 Received 16 October 1995/Accepted 30 March 1996 Membrane-containing extracts isolated from tobacco plants infected with the plus-strand RNA virus, potato virus X (PVX), supported synthesis of four major, high-molecular-weight PVX RNA products (R1 to R4). Nuclease digestion and hybridization studies indicated that R1 and R2 are a mixture of partially single- stranded replicative intermediates and double-stranded replicative forms. R3 and R4 are double-stranded products containing sequences typical of the two major PVX subgenomic RNAs. The newly synthesized RNAs were demonstrated to have predominantly plus-strand polarity. Synthesis of these products was remarkably stable in the presence of ionic detergents. Potato virus X (PVX), the type member of the Potexvirus tracts were derived from Nicotiana tabacum leaves at 7 days genus, is a flexuous rod-shaped particle containing a single, postinoculation by a procedure similar to that described by genomic RNA of 6.4 kb that is capped and polyadenylated (21, Lurie and Hendrix (15). Inoculated and upper leaves (50 g) 27). Of the five open reading frames (ORFs), the first encodes were homogenized in 150 ml of buffer I (50 mM Tris-HCl [pH a 165-kDa protein (P1) that has homology to other known 7.5], 250 mM sucrose, 5 mM MgCl2, 1 mM EDTA, 10 mM RNA-dependent RNA polymerase (RdRp) proteins (23).
    [Show full text]