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Spectrin beta III (SPTBN2) Mouse Monoclonal Antibody [Clone SPTBN2/1584]

Catalog No Format Size Price (USD)

6712-MSM4-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00

6712-MSM4-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 459.00

6712-MSM4-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 459.00

Human ID 6712 Immunogen Recombinant fragment (around aa356-475) of human SPTBN2 (exact sequence is proprietary) Human SwissProt O15020 Host / Ig Isotype Mouse / IgG1, kappa Human Unigene 26915 Mol. Weight of Antigen 246kDa Human Gene Symbol SPTBN2 Cellular Localization Cell Surface Human 11q13.2 Location Species Reactivity Human. Rat. Mouse.

Synonyms Beta III ; SCA5; Spectrin beta chain brain 2; Spectrin Positive Control PANC-1, HeLa cells. Pancreas or Liver. beta non-erythrocytic 2; Spectrin non-erythroid beta chain 2; 5; SPTBN2

Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta III Monoclonal Antibody (SPTBN2/1584).

Specificity & Comments Supplied As Spectrin is an binding protein that is a major component of the plasma 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. membrane skeleton. function as membrane organizers and Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available stabilizers by forming dimers, tetramers and higher polymers. Vertebrate WITHOUT BSA & azide at 1.0mg/ml. spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of Storage and Stability function. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 expressed in nervous tissues with highest expression levels in the to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS cerebellum, where it is found in Purkinje cell soma and dendrites. required.

Known Applications & Suggested Dilutions Limitations This antibody is available for research use only and is not approved for use in diagnosis.

Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Warranty Western Blot (1-2ug/ml) There are no warranties, expressed or implied, which extend beyond this Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) description. Company is not liable for any personal injury or economic (Staining of formalin-fixed tissues requires heating tissue sections in 10mM loss resulting from this product. Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Key References

1. Coelman, T.R., et al. J.S. 1989. Functional diversity among spectrin isoforms. Cell Motil. 12: 225-247. 2. Speicher, D.W. 1986. The present status of erythrocyte spectrin structure: the 106-residue repetitive structure is a basic feature of an entire class of . J. Cell. Biochem. 30: 245-258.

NBT01584