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Where Nitrite Respiration Meets Electrotrophy: Diversity Studies and Functional Characterization of Autotrophic Bacterial Isolates from Bioelectrochemical Systems

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Where Nitrite Respiration Meets Electrotrophy: Diversity Studies and Functional Characterization of Autotrophic Bacterial Isolates from Bioelectrochemical Systems WHERE NITRITE RESPIRATION MEETS ELECTROTROPHY: DIVERSITY STUDIES AND FUNCTIONAL CHARACTERIZATION OF AUTOTROPHIC BACTERIAL ISOLATES FROM BIOELECTROCHEMICAL SYSTEMS Ariadna Vilar Sanz Per citar o enllaçar aquest document: Para citar o enlazar este documento: Use this url to cite or link to this publication: http://hdl.handle.net/10803/383756 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices. WARNING. Access to the contents of this doctoral thesis and its use must respect the rights of the author. It can be used for reference or private study, as well as research and learning activities or materials in the terms established by the 32nd article of the Spanish Consolidated Copyright Act (RDL 1/1996). Express and previous authorization of the author is required for any other uses. In any case, when using its content, full name of the author and title of the thesis must be clearly indicated. Reproduction or other forms of for profit use or public communication from outside TDX service is not allowed. Presentation of its content in a window or frame external to TDX (framing) is not authorized either. These rights affect both the content of the thesis and its abstracts and indexes. Doctoral thesis Where nitrite respiration meets electrotrophy: Diversity studies and functional characterization of autotrophic bacterial isolates from bioelectrochemical systems Ariadna Vilar Sanz 2015 Doctoral thesis Where nitrite respiration meets electrotrophy: Diversity studies and functional characterization of autotrophic bacterial isolates from bioelectrochemical systems Ariadna Vilar Sanz 2015 Programa de doctorat: Ciències Experimentals i Sostenibilitat Dirigida per: Dr. Lluís Bañeras Vives Memòria presentada per optar al títol de doctor per la Universitat de Girona El Dr. Lluís Bañeras Vives, professor titular del departament de Biologia de la Universitat de Girona. DECLARA: Que el treball titulat “Where nitrite respiration meets electrotrophy: Diversity studies and functional characterization of autotrophic bacterial isolates from bioelectrochemical systems”, que presenta l’Ariadna Vilar Sanz per a l’obtenció del títol de doctor per la Universitat de Girona, ha estat realitzat sota la meva direcció i que compleix els requisits per poder optar a Menció Internacional. I, perquè així consti i tingui els efectes oportuns, signo aquest document. Dr. Lluís Bañeras Vives Girona, 2015 Dedicatòria Tothom diu que la recta final d’escriure una tesi és dura, però crec que fins que no t’hi trobes no ets capaç d’adonar-te’n. Abans de començar a agrair a tots els que m’heu fet costat en aquest camí, permeteu-me que em posi una mica filosòfica. No sé si és pel fet d’haver escrit la tesi, o tot el que he viscut en aquest temps, el que fa que ara ho consideri com un llarg viatge, amb tots els seus alts i baixos. Potser el més apropiat és fer el símil amb la tempesta de sorra de la que parla Murakami, de la qual sobrevius però en surts transformat. “...Y cuando la tormenta de arena haya pasado, tú no comprenderás cómo has logrado cruzarla con vida. ¡No! Ni siquiera estarás seguro de que la tormenta haya cesado de verdad. Pero una cosa sí quedará clara. Y es que la persona que surja de la tormenta no será la misma persona que penetró en ella. Y ahí estriba el significado de la tormenta de arena." Kafka en la orilla Haruki Murakami En primer lloc, gràcies, Catxo. Ja han passat uns quants anys des d’aquelles pràctiques en empresa on em vaig iniciar en el món de la recerca, i que van ser l’inici d’aquesta aventura. Gràcies per haver-me guiat en aquest viatge per convertir-me en una investigadora! Són tantes coses per les que t’he d’agrair… totes les correccions d’articles, presentacions, tesi,... en especial per aquelles fetes a corre-cuita! Però també per tot el que m’has ensenyat: a ser més crítica, resolutiva, a qüestionar-me les coses,... Gràcies per tot! Als LEQUIA, Jesús i Marilós, gràcies per apretar-me per acabar, ara sí que és de debò! Ara seriosament, gràcies per les llargues reunions discutint els resultats i decidir com enfocar els articles, i per fer que aquest treball sigui possible. A en Sebas, per la seva ajuda en la realització d’una gran part d’aquest treball, responent a tots els dubtes amb un somriure, estant disposat a discutir resultats, i sempre donant-me ànims. I per descomptat, moltes gràcies Narcís! Per tenir una paciència infinita quan he estat histèrica abans d’un congrés, així com per respondre sempre tots els dubtes, revisar els articles i per les xerrades sobre com discutir els resultats. Als micros, Bo, Frederic, Xavi, Jesús i Carles, gràcies per tots els consells que m’heu donat durant aquests anys, m’han ajudat, tant en la recerca com en la docència. I no només això, sinó també per totes les bones estones viscudes: els dinars de nadal, d’estiu, calçotades, i el tió de St Jordi! Als peke i no-tant-pekemicros: per totes les CODLs i quedades vàries, que fan que això sigui més que una feina! Però, a més, us he de dir gràcies a cadascú de vosaltres… Olaya, per ensenyar-me com treballar al laboratori quan estaves a la recta final de la tesi. Laia, per totes les estones de labo i per estar sempre disposada a donar un cop de mà. Marc, Marga, Anna H., Gaxi, Tri, i Gela, que tot i ser els “veteranus”, sempre m’heu fet sentir com una més! Rosalia, Mire, i Arantxa, pels moments de despatx, congressos, i viatges! Miri, Elena i Imma, per preguntar sempre com estaba amb un somriure, i sempre - I - estar disposades a fer una birra. Sara, per estar sempre allà, per poder compartir les alegries, les penes, i les hores de labo! A tots els becaris, doctors, Dolors, Mariona, Anna, Cristina, Marta, David, Ariadna, Pere, Roger, Sònia, Vicky i David; i als quasi doctors, Pau, Luís, Sandra, Txell, Santi, i Montse, per totes les hores de dinar, els sopars, les birres, les xerrades, les excursions, i els viatges! Això fa que els anys de la tesi siguin especials! Thank you to LabMET people, Siegfried and Nico to let me stay in your research group. To Pilar, Ana, Baharak, Joeri, and Gio, for all the beers and dinners, and of course for all the good memories from Ghent. Stephen, thanks for all the talks and advices, and also to review this thesis… It’s difficult to find words to thank you. Als meus nous companys de DI, a l’Alberto per haver-me donat l’oportunitat de començar aquesta nova etapa. A la Marta, la Clara, la Thais i l’Albert, per tot el que m’ensenyeu cada dia i per fer que la recta final de la tesi hagi estat més suportable. A les nenes, Gisela, Astrid, Nuria, Olga, Mariona, Judit, Ester i Laia, per els somriures i l’ajuda quan vaig perduda pel laboratori. A les compis de cotxe, Gemma, Sònia i Laura, per les hores de conversa i rialles, anant i tornant de nit! I a tots, gràcies per fer-me sentir com una més des del primer dia! A la meva família, al meu avi, i les meves ties, Pili i Tere, per estar sempre preocupats per mi, per ajudar-me en els moments durs i per els dinars tant sorollosos dels diumenges! Gràcies per estar sempre allà, ja sé que no us ho dic massa sovint! A la meva mare, Tina, gràcies per ser un exemple, sempre animada i amb un somriure. Per sempre preocupar-te per mi, animar-me en els moments difícils, i estar allà pel que faci falta! A la iaia, perquè ser que t’hagués agradat veure que al final si que l’he acabat! He de tenir un record especial pel meu pare, perquè sé que sempre vas confiar en mi, en que seria capaç de fer qualsevol cosa que em proposes.
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