Where Nitrite Respiration Meets Electrotrophy: Diversity Studies and Functional Characterization of Autotrophic Bacterial Isolates from Bioelectrochemical Systems
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Themothrrjc Azorensis Sp. Nov., an Obligately Chemolithoautotrophic, Sulfur-Oxidizing, Thermophilic Bacterium? ELENA V
INTERNATIONALJOURNAL OF SYSTEMATICBACTERIOLOGY, Apr. 1996, p. 422-428 Vol. 46, No. 2 0020-7713/96/$04.00+ 0 Copyright 0 1996, International Union of Microbiological Societies Themothrrjc azorensis sp. nov., an Obligately Chemolithoautotrophic, Sulfur-Oxidizing, Thermophilic Bacterium? ELENA V. ODINTSOVA,l$ HOLGER W. JA”ASCH,l* J. ANTHONY MAMONE,2 AND THOMAS A. LANGWORTHY3 Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, U.S. Biochemical Corporation, Cleveland, Ohio 44128, and University of South Dakota, Vennillion, South Dakota 570693 A new aerobic, obligately chemolithoautotrophic, thermophilic, sulfur-oxidizing bacterium, Thermothrix azorensis, was isolated from a hot spring on Sao Miguel Island in the Azores. The cells of this organism are gram negative, nonsporulating, and rod shaped. Filament formation appears to occur as a response to nonoptimal growth conditions. Growth occurs at 63 to 86”C, and the optimum temperature is 76 to 78°C. The optimum pH range for growth is 7.0 to 7.5. The G+C content of the DNA of our isolate is 39.7 mol%. This isolate uses thiosulfate, tetrathionate, hydrogen sulfide, and elemental sulfur as energy sources. Of particular interest are the absence of Calvin cycle enzymes and the initial appearance of sulfide during the lag phase of growth of aerobic cultures grown on elemental sulfur. The subsequent formation of thiosulfate is followed by oxidation of the thiosulfate to sulfate. T. azorensis differs from the only other Thermothrix species that has been described, Thermothrix thiopura, by having higher optimum and maximum growth temperatures, by being an obligate chemolithoautotroph, and by its close but separate position on a 16s rRNA sequence-based phyloge- netic tree. -
Changes in Bacterioplankton Communities Resulting from Direct and Indirect Interactions with Trace Metal Gradients in an Urbaniz
Changes in Bacterioplankton Communities Resulting From Direct and Indirect Interactions With Trace Metal Gradients in an Urbanized Marine Coastal Area Clément Coclet, Cédric Garnier, Gaël Durrieu, Dario Omanović, Sébastien d’Onofrio, Christophe Le Poupon, Jean-Ulrich Mullot, Jean-François Briand, Benjamin Misson To cite this version: Clément Coclet, Cédric Garnier, Gaël Durrieu, Dario Omanović, Sébastien d’Onofrio, et al.. Changes in Bacterioplankton Communities Resulting From Direct and Indirect Interactions With Trace Metal Gradients in an Urbanized Marine Coastal Area. Frontiers in Microbiology, Frontiers Media, 2019, 10, 10.3389/fmicb.2019.00257. hal-02049263 HAL Id: hal-02049263 https://hal.archives-ouvertes.fr/hal-02049263 Submitted on 26 Feb 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. fmicb-10-00257 February 20, 2019 Time: 17:14 # 1 ORIGINAL RESEARCH published: 22 February 2019 doi: 10.3389/fmicb.2019.00257 Changes in Bacterioplankton Communities Resulting From Direct and Indirect Interactions With Trace Metal Gradients in an Urbanized -
Thiobacillus Denitrificans
Nitrate-Dependent, Neutral pH Bioleaching of Ni from an Ultramafic Concentrate by Han Zhou A thesis submitted in conformity with the requirements for the degree of Master of Applied Science Chemical Engineering and Applied Chemistry University of Toronto © Copyright by Han Zhou 2014 ii Nitrate-Dependent, Neutral pH Bioleaching of Ni from an Ultramafic Concentrate Han Zhou Master of Applied Science Chemical Engineering and Applied Chemistry University of Toronto 2014 Abstract This study explores the possibility of utilizing bioleaching techniques for nickel extraction from a mixed sulfide ore deposit with high magnesium content. Due to the ultramafic nature of this material, well-studied bioleaching technologies, which rely on acidophilic bacteria, will lead to undesirable processing conditions. This is the first work that incorporates nitrate-dependent bacteria under pH 6.5 environments for bioleaching of base metals. Experiments with both defined bacterial strains and indigenous mixed bacterial cultures were conducted with nitrate as the electron acceptor and sulfide minerals as electron donors in a series of microcosm studies. Nitrate consumption, sulfate production, and Ni released into the aqueous phase were used to track the extent of oxidative sulfide mineral dissolution; taxonomic identification of the mixed culture community was performed using 16S rRNA gene sequencing. Nitrate-dependent microcosms that contained indigenous sulfur- and/or iron-oxidizing microorganisms were cultured, characterized, and provided a proof-of-concept basis for further bioleaching studies. iii Acknowledgments I would like to extend my most sincere gratitude toward both of my supervisors Dr. Vladimiros Papangelakis and Dr. Elizabeth Edwards. This work could not have been completed without your brilliant and patient guidance. -
Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms
Hindawi Publishing Corporation International Journal of Microbiology Volume 2012, Article ID 156537, 8 pages doi:10.1155/2012/156537 Research Article Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms Elisa Korenblum,1 Diogo Bastos Souza,1 Monica Penna,2 and Lucy Seldin1 1 Laborat´orio de Gen´etica Microbiana, Instituto de Microbiologia Prof. Paulo de G´oes, Universidade Federal do Rio de Janeiro, Centro de Ciˆencias da Sa´ude, Bloco I, Ilha do Fund˜ao, 21941-590 Rio de Janeiro, RJ, Brazil 2 Gerˆencia de Biotecnologia e Tratamentos Ambientais, CENPES-PETROBRAS, Ilha do Fund˜ao, 21949-900 Rio de Janeiro, RJ, Brazil Correspondence should be addressed to Lucy Seldin, [email protected] Received 18 April 2011; Revised 11 June 2011; Accepted 13 October 2011 Academic Editor: J. Wiegel Copyright © 2012 Elisa Korenblum et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Crude oil samples with high- and low-water content from two offshore platforms (PA and PB) in Campos Basin, Brazil, were assessed for bacterial communities by 16S rRNA gene-based clone libraries. RDP Classifier was used to analyze a total of 156 clones within four libraries obtained from two platforms. The clone sequences were mainly affiliated with Gammaproteobacteria (78.2% of the total clones); however, clones associated with Betaproteobacteria (10.9%), Alphaproteobacteria (9%), and Firmicutes (1.9%) were also identified. Pseudomonadaceae was the most common family affiliated with these clone sequences. -
Downloaded 13 April 2017); Using Diamond
bioRxiv preprint doi: https://doi.org/10.1101/347021; this version posted June 14, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 2 3 4 5 Re-evaluating the salty divide: phylogenetic specificity of 6 transitions between marine and freshwater systems 7 8 9 10 Sara F. Pavera, Daniel J. Muratorea, Ryan J. Newtonb, Maureen L. Colemana# 11 a 12 Department of the Geophysical Sciences, University of Chicago, Chicago, Illinois, USA 13 b School of Freshwater Sciences, University of Wisconsin Milwaukee, Milwaukee, Wisconsin, USA 14 15 Running title: Marine-freshwater phylogenetic specificity 16 17 #Address correspondence to Maureen Coleman, [email protected] 18 bioRxiv preprint doi: https://doi.org/10.1101/347021; this version posted June 14, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 19 Abstract 20 Marine and freshwater microbial communities are phylogenetically distinct and transitions 21 between habitat types are thought to be infrequent. We compared the phylogenetic diversity of 22 marine and freshwater microorganisms and identified specific lineages exhibiting notably high or 23 low similarity between marine and freshwater ecosystems using a meta-analysis of 16S rRNA 24 gene tag-sequencing datasets. As expected, marine and freshwater microbial communities 25 differed in the relative abundance of major phyla and contained habitat-specific lineages; at the 26 same time, however, many shared taxa were observed in both environments. 27 Betaproteobacteria and Alphaproteobacteria sequences had the highest similarity between 28 marine and freshwater sample pairs. -
Evaluating the Biogeochemistry and Microbial Function in the Athabasca Oil Sands Region: Understanding Natural Baselines for Reclamation End-Points
University of Windsor Scholarship at UWindsor Electronic Theses and Dissertations Theses, Dissertations, and Major Papers 2019 Evaluating the biogeochemistry and microbial function in the Athabasca Oil Sands region: Understanding natural baselines for reclamation end-points Thomas Reid University of Windsor Follow this and additional works at: https://scholar.uwindsor.ca/etd Recommended Citation Reid, Thomas, "Evaluating the biogeochemistry and microbial function in the Athabasca Oil Sands region: Understanding natural baselines for reclamation end-points" (2019). Electronic Theses and Dissertations. 7732. https://scholar.uwindsor.ca/etd/7732 This online database contains the full-text of PhD dissertations and Masters’ theses of University of Windsor students from 1954 forward. These documents are made available for personal study and research purposes only, in accordance with the Canadian Copyright Act and the Creative Commons license—CC BY-NC-ND (Attribution, Non-Commercial, No Derivative Works). Under this license, works must always be attributed to the copyright holder (original author), cannot be used for any commercial purposes, and may not be altered. Any other use would require the permission of the copyright holder. Students may inquire about withdrawing their dissertation and/or thesis from this database. For additional inquiries, please contact the repository administrator via email ([email protected]) or by telephone at 519-253-3000ext. 3208. Evaluating the biogeochemistry and microbial function in the Athabasca Oil Sands -
In Situ Electrochemical Studies of the Terrestrial Deep Subsurface Biosphere at the Sanford
bioRxiv preprint doi: https://doi.org/10.1101/555474; this version posted February 20, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 In Situ Electrochemical Studies of the Terrestrial Deep Subsurface Biosphere at the Sanford 2 Underground Research Facility, South Dakota, USA 3 4 Yamini Jangir,a Amruta A. Karbelkar,b Nicole M. Beedle,c Laura A. Zinke,d Greg Wanger,d 5 Cynthia M. Anderson,e Brandi Kiel Reese,f Jan P. Amend,c,d and Mohamed Y. El-Naggar,a,b,c#, 6 7 Department of Physics and Astronomy, University of Southern California, Los Angeles, 8 California, USAa; 9 Department of Chemistry, University of Southern California, Los Angeles, California, USAb; 10 Department of Biological Sciences, University of Southern California, Los Angeles, California, 11 USAc; 12 Department of Earth Science, University of Southern California, Los Angeles, California, USAd; 13 Center for the Conservation of Biological Resources, Black Hills State University, Spearfish, 14 South Dakota, USAe 15 Department of Life Sciences, Texas A&M University, Corpus Christi, Texas, USAf; 16 17 Running Head: [limit: 54 characters and spaces] 18 19 #Address correspondence to Mohamed Y. El-Naggar, [email protected]. 20 21 22 1 bioRxiv preprint doi: https://doi.org/10.1101/555474; this version posted February 20, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. -
Draft Genome Sequence of the Nitrogen-Fixing Endophyte Azoarcus Communis Swub3
Lawrence Berkeley National Laboratory Recent Work Title Draft Genome Sequence of the Nitrogen-Fixing Endophyte Azoarcus communis SWub3. Permalink https://escholarship.org/uc/item/2cd6v88c Journal Microbiology resource announcements, 7(13) ISSN 2576-098X Authors Zorraquino, Violeta Toubiana, David Yan, Dawei et al. Publication Date 2018-10-04 DOI 10.1128/mra.01080-18 Peer reviewed eScholarship.org Powered by the California Digital Library University of California GENOME SEQUENCES crossm Draft Genome Sequence of the Nitrogen-Fixing Endophyte Azoarcus communis SWub3 Violeta Zorraquino,a David Toubiana,a Dawei Yan,a Eduardo Blumwalda aDepartment of Plant Sciences, University of California, Davis, California, USA ABSTRACT Here we report a draft genome sequence of Azoarcus communis SWub3, a nitrogen-fixing bacterium isolated from root tissues of Kallar grass in Pakistan. iological nitrogen fixation is a process in which a living organism reduces atmo- Bspheric dinitrogen into two NH3 molecules. This reaction is catalyzed by the nitrogenase complex present exclusively in Bacteria and Archaea species. Plants can benefit from biological nitrogen fixation when they are in association with these nitrogen-fixing prokaryotes, either free living or as symbionts associated with their roots. Azoarcus is a bacterial genus that comprises species isolated from different environments, such as plant roots, sediments, aquifers, and contaminated soil (1–4). All Azoarcus species are Gram-negative rods with a strictly aerobic metabolism that can fix nitrogen microaerobically. The interest in this bacterial genus resides in its ability to efficiently infect several crops, including rice, which is a food staple for more than half of the world’s population (5–7). -
Applied Microbiology VOLUME 60 This Page Intentionally Left Blank ADVANCES in Applied Microbiology
ADVANCES IN Applied Microbiology VOLUME 60 This page intentionally left blank ADVANCES IN Applied Microbiology Edited by ALLEN I. LASKIN Somerset, New Jersey SIMA SARIASLANI Wilmington, Delaware GEOFFREY M. GADD Dundee, United Kingdom VOLUME 60 AMSTERDAM • BOSTON • HEIDELBERG • LONDON NEW YORK • OXFORD • PARIS • SAN DIEGO SAN FRANCISCO • SINGAPORE • SYDNEY • TOKYO Academic Press is an imprint of Elsevier Academic Press is an imprint of Elsevier 525 B Street, Suite 1900, San Diego, California 92101-4495, USA 84 Theobald’s Road, London WC1X 8RR, UK This book is printed on acid-free paper. Copyright ß 2006, Elsevier Inc. All Rights Reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the Publisher. The appearance of the code at the bottom of the first page of a chapter in this book indicates the Publisher’s consent that copies of the chapter may be made for personal or internal use of specific clients. This consent is given on the condition, however, that the copier pay the stated per copy fee through the Copyright Clearance Center, Inc. (www.copyright.com), for copying beyond that permitted by Sections 107 or 108 of the U.S. Copyright Law. This consent does not extend to other kinds of copying, such as copying for general distribution, for advertising or promotional purposes, for creating new collective works, or for resale. Copy fees for pre-2006 chapters are as shown on the title pages. If no fee code appears on the title page, the copy fee is the same as for current chapters. -
Methylene Blue Decolorizing Bacteria Isolated from Water Sewage in Yogyakarta, Indonesia
BIODIVERSITAS ISSN: 1412-033X Volume 21, Number 3, March 2020 E-ISSN: 2085-4722 Pages: 1136-1141 DOI: 10.13057/biodiv/d210338 Methylene blue decolorizing bacteria isolated from water sewage in Yogyakarta, Indonesia MICHELLE, RACHEL ARVY NABASA SIREGAR, ASTIA SANJAYA, JAP LUCY, REINHARD PINONTOAN Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Lippo Karawaci, Tangerang 15811, Banten, Indonesia. Tel./Fax. +62-21-5460901, email: [email protected] Manuscript received: 11 December 2019. Revision accepted: 20 February 2020. Abstract. Michelle, Siregar RAN, Sanjaya A, Jap L, Pinontoan R. 2020. Methylene blue decolorizing bacteria isolated from water sewage in Yogyakarta, Indonesia. Biodiversitas 21: 1136-1141. The textile industry contributes to water pollution issues all over the world. One of the most commonly applied cationic dye in the textile industry is methylene blue. This study aimed to isolate bacteria with the potential to decolorize methylene blue from dye contaminated sewage water located in Kulon Progo District, Yogyakarta, where several textile industries within the proximity, are located. Characterizations of bacterial candidates were done morphologically and biochemically. Molecular identification was conducted by 16S rRNA sequencing. The ability of isolates to decolorize methylene blue was observed by the reduction of methylene blue’s maximum absorption at the wavelength of 665 nm. The results showed that isolates were identified as Comamonas aquatica and Ralstonia mannitolilytica. C. aquatica PMB-1 and R. mannitolilytica PMB-2 isolates were able to decolorize methylene blue with decolorization percentage of 67.9% and 60.3%, respectively when incubated for 96 hours at 37°C. -
Microbial Ecology of Denitrification in Biological Wastewater Treatment
water research 64 (2014) 237e254 Available online at www.sciencedirect.com ScienceDirect journal homepage: www.elsevier.com/locate/watres Review Microbial ecology of denitrification in biological wastewater treatment * ** Huijie Lu a, , Kartik Chandran b, , David Stensel c a Department of Civil and Environmental Engineering, University of Illinois at Urbana Champaign, 205 N Mathews, Urbana, IL 61801, USA b Department of Earth and Environmental Engineering, Columbia University, 500 West 120th Street, New York, NY 10027, USA c Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195, USA article info abstract Article history: Globally, denitrification is commonly employed in biological nitrogen removal processes to Received 21 December 2013 enhance water quality. However, substantial knowledge gaps remain concerning the overall Received in revised form community structure, population dynamics and metabolism of different organic carbon 26 June 2014 sources. This systematic review provides a summary of current findings pertaining to the Accepted 29 June 2014 microbial ecology of denitrification in biological wastewater treatment processes. DNA Available online 11 July 2014 fingerprinting-based analysis has revealed a high level of microbial diversity in denitrifica- tion reactors and highlighted the impacts of carbon sources in determining overall deni- Keywords: trifying community composition. Stable isotope probing, fluorescence in situ hybridization, Wastewater denitrification microarrays and meta-omics further -
Diverse Environmental Pseudomonas Encode Unique Secondary Metabolites That Inhibit Human Pathogens
DIVERSE ENVIRONMENTAL PSEUDOMONAS ENCODE UNIQUE SECONDARY METABOLITES THAT INHIBIT HUMAN PATHOGENS Elizabeth Davis A Thesis Submitted to the Graduate College of Bowling Green State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE August 2017 Committee: Hans Wildschutte, Advisor Ray Larsen Jill Zeilstra-Ryalls © 2017 Elizabeth Davis All Rights Reserved iii ABSTRACT Hans Wildschutte, Advisor Antibiotic resistance has become a crisis of global proportions. People all over the world are dying from multidrug resistant infections, and it is predicted that bacterial infections will once again become the leading cause of death. One human opportunistic pathogen of great concern is Pseudomonas aeruginosa. P. aeruginosa is the most abundant pathogen in cystic fibrosis (CF) patients’ lungs over time and is resistant to most currently used antibiotics. Chronic infection of the CF lung is the main cause of morbidity and mortality in CF patients. With the rise of multidrug resistant bacteria and lack of novel antibiotics, treatment for CF patients will become more problematic. Escalating the problem is a lack of research from pharmaceutical companies due to low profitability, resulting in a large void in the discovery and development of antibiotics. Thus, research labs within academia have played an important role in the discovery of novel compounds. Environmental bacteria are known to naturally produce secondary metabolites, some of which outcompete surrounding bacteria for resources. We hypothesized that environmental Pseudomonas from diverse soil and water habitats produce secondary metabolites capable of inhibiting the growth of CF derived P. aeruginosa. To address this hypothesis, we used a population based study in tandem with transposon mutagenesis and bioinformatics to identify eight biosynthetic gene clusters (BGCs) from four different environmental Pseudomonas strains, S4G9, LE6C9, LE5C2 and S3E10.