CURRICULUM VITAE

Young Chang SOHN (Ph.D. / Associate Professor)

Phone & Fax :+82-33-640-2348 E-mail; [email protected] Address: Department of Marine Molecular BioTechnology College of Life Sciences Gangneung-Wonju National University 120 Gangneung Daehangno, Gangneung, Gangwon Province 210-702, Republic of Korea

Education and Research Experience 1993. 2, B.S. in Dept. Aquaculture, Pukyung Nat’l Univ., Korea (Advisor: Prof. Y. J. Chang) 1997. 3, M.S. in Aquatic Biological Science, University of Tokyo, Japan (Co-Advisors: Profs. M. Kobayashi and K. Aida) 2000. 3, Ph.D. in Aquatic Bioscience, University of Tokyo, Japan (Co-Advisors: Profs. M. Kobayashi and K. Aida) (Dissertation: Molecular endocrinological studies on expression of gonadotropin and thyrotropin genes in goldfish) 2000. 4 - 2002. 2, Postdoc Fellow at Center for Ligand and Transcription, Chonnam Nat’l Univ., and POSTECH, Korea (Mentor: Prof. Jae W. Lee) 2002. 3. - present. Faculty at Gangneung-Wonju Nat’l Univ., Korea

Professional Experience Ad-hoc Reviewer of Journal of Molecular Endocrinology, Molecular and Cellular Endocrinology, PLoS One, General and Comparative Endocrinology, Molecular Reproduction and Development, Molecular Biology Reports, BMC Structural Biology, Journal of Experimental Zoology, Fish Physiology and Biochemistry, Comparative Biochemistry and Physiology, Zoological Science, Fisheries Science, Canadian Journal of Zoology, etc.

Research Interests 1. Role of transcriptional regulators in nuclear receptor action 2. Environmental effects on endocrinological bio-system 3. Hormonal regulation for reproductive endocrine system 4. Molecular mechanisms by which hormonal action affects embryonic and cancer cells 5. Novel ligand search involved in cholesterol metabolism

Research Methods Molecular cloning (Genome and cDNA), Western-Northern-Southern blotting, Primary cell culture, Yeast cell culture, Yeast Two-hybrid assay, Transfection for mammalian cells, Mammal Two-hybrid assay, GST Pull-down assay, Immuno- precipitation, Chromatin immuno-precipitation, Protein expression in bacterial and yeast cells, Radioimmunoassay, Immuno-histochemistry, Animal care (fish)

Publications 1. Chiba A, Sohn YC, Oka S, Honma Y (1996). Localization of neuropeptide Y-like immunoreactivity in the saccus vasculosus of cartilaginous and bony fishes. Zool. Sci., 13, 347-350.

2. Chiba A, Sohn YC, Honma Y (1996). Immunohistochemical and ultrastructural characterization of the terminal nerve ganglion cells of the ayu, Plecoglossus altivelis (Salmoniformes, Teleostei). Anat. Rec., 246, 549-556.

3. Chiba A, Sohn YC, Honma Y (1996). Distribution of neuropeptide Y and gonadotropin-releasing hormone immunoreactivities in the brain and hypophysis of the ayu, Plecoglossus altivelis(Teleostei). Arch. Histol. Cytol., 59, 137-148.

4. Kobayashi M, Amano M, Kim M-H, Yoshiura Y, Sohn YC, Suetake H, Aida K (1997). Gonadotropin-releasing hormone and gonadotropin in goldfish and masu salmon. Fish Physiol. Biochem., 17, 1-8.

5. Sohn YC, Yoshiura Y, Kobayashi M, Aida K (1998). Effects of water temperature and food limitation on pituitary gonadotropin and thyrotropin subunit mRNA levels in the goldfish Carassius auratus. Fisheries Sci., 64, 700-706.

6. Sohn YC, Yoshiura Y, Kobayashi M, Aida K (1998). Effect of sex steroids on the mRNA levels of gonadotropin I and II subunits in the goldfish Carassius auratus. Fisheries Sci., 64, 715-721.

7. Sohn YC, Suetake H, Yoshiura Y, Kobayashi M, Aida K (1998). Structural and expression analyses of gonadotropin Iβ subunit genes in goldfish (Carassius auratus). Gene, 222, 257-267.

8. Kwon JY, Chang YJ, Sohn YC, Aida K (1999). Plasma and ovarian thyroxine levels in relation to sexual maturation and gestation in female Sebastes inermis. J. Fish Biol., 54, 370-379.

9. Sohn YC, Yoshiura Y, Kobayashi M, Aida K (1999). Seasonal changes in mRNA levels of gonadotropin and thyrotropin subunits in the goldfish, Carassius auratus. Gen. Comp. Endocrinol., 113, 436-444.

10. Sohn YC, Yoshiura Y, Suetake H, Kobayashi M, Aida K (1999). Isolation and characterization of the goldfish thyrotropin β subunit gene including the 5'-flanking region. Gen. Comp. Endocrinol., 115, 463-473.

11. Yoshiura Y, Sohn YC, Munakata A, Kobayashi M, Aida K (1999). Molecular cloning of the cDNA encoding the β subunit of thyrotropin and regulation of its gene expression by thyroid hormones in the goldfish, Carassius auratus. Fish Physiol. Biochem., 21, 201-210.

12. Sohn YC, Yoshiura Y, Suetake H, Kobayashi M, Aida K (1999). Nucleotide sequence of gonadotropin II subunit gene in goldfish. Fisheries Sci., 65, 800-801.

13. Kobayashi M, Sohn YC, Yoshiura Y, Aida K (2000). Effects of sex steroids on the mRNA levels of gonadotropin subunits in juvenile and ovariectomized goldfish Carassius auratus. Fisheries Sci., 66, 223-231.

14. Sohn YC, Kobayashi M, Aida K (2001). Regulation of gonadotropin beta subunit gene expression by testosterone and gonadotropin-releasing hormones in the goldfish, Carassius auratus. Comp. Biochem. Physiol. B, 129, 419-426.

15. Jang MK, Goo YH, Sohn YC, Kim YS, Lee SK, Kang H, Cheong J, Lee JW (2001). Ca2+/calmodulin-dependent protein kinase IV stimulates nuclear factor-κB transactivation via phosphorylation of the p65 subunit. J. Biol. Chem., 276, 20005- 20010.

16. Sohn YC*, Kwak E*, Na Y, Lee JW, Lee SK (2001). Silencing mediator of retinoid and thyroid hormone receptors and activating signal cointegrator-2 as transcriptional coregulators of the orphan nuclear receptor nur77. J. Biol. Chem., 276, 43734-43739. (*equal contribution)

17. Kim SW, Cheong C, Sohn YC, Goo YH, Oh WJ, Park JH, Joe SY, Kang HS, Kim DK, Kee C, Lee JW, Lee HW (2002). Multiple developmental defects derived from impaired recruitment of ASC-2 to nuclear receptors in mice: Implication for posterior lenticonus with cataract. Mol. Cell. Biol., 22, 8409-8414.

18. Goo YH*, Sohn YC*, Kim DH*, Kim SW, Kang MJ, Jung DJ, Kwak E, Barlev NA, Berger SL, Chow VT, Roeder RG, Azorsa DO, Meltzer PS, Suh PG, Song EJ, Lee KJ, Lee YC, Lee JW (2003). Activating signal cointegrator 2 belongs to a novel steady-state complex that contains a subset of trithorax group proteins. Mol. Cell. Biol., 23, 140-149.(*equal contribution)

19. Sohn YC*, Kim SW, Lee S, Kong YY, Na DS, Lee SK, Lee JW* (2003). Dynamic inhibition of nuclear receptor activation by corepressor binding. Mol. Endocrinol. 17, 366-372. (*Co-corresponding authors)

20. Ge W, Ko N-L, Pang F. Y-M, Chung M-F, Lin S-W, Yuen C-Y, Lau M-T, Lin L, Sohn YC, Kobayashi M, Aida K (2003). Activin stimulates goldfish FSH biosynthesis by enhancing FSHβ promoter activity. Fish Physiol. Biochem., 28, 65- 71.

21. Choi EJ, Jin DH, Sohn YC (2003). Gonadotropin in the Manchurian trout, Brachymystax lenok tsinlingesis. Fish Physiol. Biochem., 28, 89-90.

22. Jung J-H, Sohn YC, Lee J-Y, Kim D-J, Lee J-S, Jeon J-K, Han C-H (2003). Molecular cloning of vitellogenin cDNA in rockfish (Sebastes schlegeli) and effects of estrogenic substances on its gene expression. Fish Physiol. Biochem., 28, 419- 420.

23. Kim D-J, Cho Y, Sohn YC (2005). Molecular characterization of rockfish (Sebastes schlegeli) gonadotropin subunits and their mRNA expression profiles during oogenesis. Gen. Comp. Endocrinol., 141, 282-290.

24. Maeng S, Jung Y, Choi E, Jeon JK, Kim S, Gen K, Sohn YC (2005). Expression of gonadotropin subunit genes following 4-nonylphenol exposure in masu salmon: Effects on transcript levels and promoter activities via estrogen receptor alpha. Comp. Biochem. Physiol. B, 142, 383-390.

25. Choi EJ, Ko H, Shin J, Kim MA, Sohn YC (2005). Expression of gonadotropin genes in Manchurian trout Brachymystax lenok and production of recombinant gonadotropins. Fisheries Sci., 71, 1193-1200.

26. Shin J, Oh D, Sohn YC (2006). Molecular characterization and expression analysis of stanniocalcin-1 in turbot (Scophthalmus maximus). Gen. Comp. Endocrinol., 147, 214-221.

27. Ko H, Park W, Kim D-J, Kobayashi M, Sohn YC (2007). Biological activities of recombinant Manchurian trout FSH and LH: their receptor specificity, steroidogenic and vitellogenic potencies. J. Mol. Endocrinol., 38, 99-111.

28. Park W, Lee CH, Lee CS, Kim D-J, Kim J-H, Tamaru CS, Sohn YC (2007). Effects of a gonadotropin-releasing hormone analog combined with pimozide on plasma sex steroid hormones, ovulation and egg quality in freshwater-exposed female chum salmon (Oncorhynchus keta). Aquaculture, 271, 488-497.

29. Yoon M, Choi YS, Jin HJ, Sohn YC, Lee SK, Jin DH (2008). Heteropolymorphism of mitochondrial NADH dehydrogenase subunit 3 gene for the population analysis of chum salmon, Oncorhynchus keta. J Environ Biol. 29(4):567-70.

30. Hayakawa Y, Morita T, Kitamura W, Kanda S, Banba A, Nagaya H, Hotta K, Sohn YC, Yoshizaki G, Kobayashi M (2008). Biological activities of single-chain goldfish follicle-stimulating hormone and luteinizing hormone. Aquaculture, 274, 408-415.

31. Shin J, Sohn YC (2008). Molecular cloning of stanniocalcin-1 and its extracorpuscular regulation by salinity and Ca2+ in Japanese flounder. Zool. Sci., 25, 728-738.

32. Park W, Kim GJ, Choi HS, Vanacker JM, Sohn YC (2009). Conserved properties of a urochordate estrogen receptor-related receptor (ERR) with mammalian ERRalpha. Biochim Biophys Acta, 1789, 125-134.

33. Shin J, Sohn YC (2009). cDNA cloning of Japanese flounder stanniocalcin 2 and its mRNA expression in a variety of tissues. Comp Biochem Physiol A Mol Integr Physiol. 153(1):24-9.

34. Kang K, Lee SB, Jung SH, Cha KH, Park WD, Sohn YC, Nho CW (2009). Tectoridin, a poor ligand of estrogen receptor alpha, exerts its estrogenic effects via an ERK-dependent pathway. Mol Cells 27(3):351-7.

35. Choi EJ, Kwon HC, Sohn YC, Nam CW, Park HB, Kim CY, Yang HO (2009). Four flavonoids from Echinosophora koreensis and their effects on alcohol metabolizing enzymes. Arch Pharm Res. 32(6):851-5.

36. Choi EJ, Kwon HC, Sohn YC, Yang HO (2010). Kistimonas asteriae gen. nov., sp. nov., a gammaproteobacterium isolated from Asterias amurensis. Int J Syst Evol Microbiol. 60(Pt 4):938-43.

37. Choi S, Lee CH, Park W, Kim DJ, Sohn YC (2010). Effects of shortened photoperiod on gonadotropin-releasing hormone, gonadotropin, and vitellogenin gene expression associated with ovarian maturation in rainbow trout. Zoolog Sci. 27(1):24-32.

38. Kobayashi M, Hayakawa Y, Park W, Banba A, Yoshizaki G, Kumamaru K, Kagawa H, Kaki H, Nagaya H, Sohn YC (2010). Production of recombinant Japanese eel gonadotropins by baculovirus in silkworm larvae. Gen Comp Endocrinol. 167(3):379-86.

39. Kim SH, Yang HO, Sohn YC, Kwon HC (2010). Aeromicrobium halocynthiae sp. nov., a taurocholic acid producing bacterium isolated from the marine ascidian Halocynthia roretzi. Int J Syst Evol Microbiol. 60(Pt 12):2793-8.

40. Yun JH, Lee SB, Lee HJ, Kim CY, Kim MA, Sohn YC, Nho CW (2010). Bi- functional induction of the quinone reductase and cytochrome P450 1A1 by youngiasides via Nrf2-ARE and AhR-XRE pathways. Biol Pharm Bull. 33(10):1650-7.

41. Kim MA, Kim GJ, Maeng S, Jin DH, Sohn YC (2011). Characterization of steroid receptor coactivator in sea urchin, Strongylocentrotus nudus, and its involvement in embryonic development. Mol Cell Endocrinol. 331(1):89-101.

42. Kim MA, Kim DS, Sohn YC (2011). Characterization of two functional glucocorticoid receptors in the marine medaka Oryzias dancena. Gen Comp Endocrinol. 171(3):341-9.

43. Maeng S, Lee JH, Kim GJ, Kim SH, Kwon HC, Shin YK, Sohn YC (2012). Molecular and expression analysis of the farnesoid X receptor in the urochordate Halocynthia roretzi. Comp Biochem Physiol B Biochem Mol Biol. 161(3):189-96.

44. Aizen J, Kobayashi M, Selicharova I, Sohn YC, Yoshizaki G, Levavi-Sivan B (2012). Steroidogenic response of carp ovaries to piscine FSH and LH depends on the reproductive phase. Gen Comp Endocrinol. 178(1):28-36.

45. Maeng S, Lee JH, Choi S, Kim MA, Shin YK, Sohn YC (2012). The retinoid X receptor in a marine invertebrate chordate: Evolutionary insights from urochordates. Gen Comp Endocrinol. 178(2):380-390.

46. Kong HJ, Lee YJ, Shin J, Cho HK, Kim WJ, Kim HS, Cheong J, Sohn YC, Lee SJ, Kim BS (2012). Molecular characterization of tripartite motif protein 25 (TRIM25) involved in ERα-mediated transcription in the Korean rose bitterling Rhodeus uyekii. Comp Biochem Physiol B Biochem Mol Biol. 163(1):147-53.

47. Kim SH, Shin YK, Sohn YC, Kwon HC (2012). Two new cholic acid derivatives from the marine ascidian-associated bacterium Hasllibacter halocynthiae. Molecules, 17(10):12357-64.

48. Aizen J, Kowalsman N, Kobayashi M, Hollander L, Sohn YC, Yoshizaki G, Niv MY, Levavi-Sivan B (2012). Experimental and computational study of inter- and intra- species specificity of gonadotropins for various gonadotropin receptors. Mol Cell Endocrinol. 364(1-2):89-100.

49. Maeng S, Kim GJ, Choi EJ, Yang HO, Lee D-S, Sohn YC (2012). 9-Cis retinoic acid induces growth inhibition in retinoid-sensitive breast cancer and sea urchin embryonic cells via retinoid X receptor α and replication factor C3. Mol Endocrinol. 26(11):1821-35.