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St. Lucie, Units 1 and 2 Florida Power & Light Company Biological Plan of Study Implementation for St. Lucie Plant EPU Post-Uprate Event 7 Data Report Report to Florida Power & Light Company June 2014 Submitted by Ecological Associates, Inc. Post Office Box 405 Jensen Beach, Florida INTRODUCTION During January 2014 and February 2014, Ecological Associates, Inc. (EAI) conducted the seventh post-uprate field sampling event in accordance with the St. Lucie Plant EPU Biological Plan of Study. Sampling was conducted on six days between January 27, 2014 and February 25, 2014. Data collected included general environmental data; water quality data; numbers and sizes of fish and shellfish collected by gill net, trawl, and beach seine; numbers of fish eggs and larvae and commercially or recreationally important decapod crustacean larvae collected by plankton net; and, numbers and sizes of sea turtles observed. Results of the seventh post-uprate sampling event are provided in this report. All data should be considered preliminary until quality assurance checks have been completed. SAMPLING LOCATIONS Sampling was conducted within three separate sampling sites: SL1 located midway between the St. Lucie Plant and the Ft. Pierce Inlet, SL2 located in the vicinity of the St. Lucie Plant cooling water discharge, and SL3 located midway between the St. Lucie Plant and the St. Lucie Inlet. For the purposes of gill netting and trawling, three transects were established within each of the three sampling sites: Transect A was located approximately 600 ft from shore on the beach terrace in water depths of 8-32 ft, Transect B was located approximately 4,000 ft. from shore in water depths of 34-45 ft, and Transect C was located approximately 8,000 ft. from shore in water depths of 31-47 ft. Plankton samples were collected concurrently with trawl samples at all three sites but only on Transects A and C. Three stations were also established within each of the three sampling sites for the purpose of beach seining: Station A was located 0.5 mi. north of the center of the site, Station B was located at the center of the site, and Station C was located 0.5 mi. south of the center of the site. At all stations, seines were pulled to the beach from a water depth of approximately 4 ft. For the purpose of boat-based sea turtle surveys, one transect was established in each of the three sampling sites. The transects were located along nearshore hardbottom habitat in each site. RESULTS Environmental data were recorded at each station during each day of sampling. Data recorded included sea conditions, air temperature, wind speed and direction, and sky conditions. Environmental data are summarized by day in Table 1. Values reflect the range of values recorded throughout each day of sampling. Water quality data were recorded at three locations and three depths along each of the nine transects established for trawl and gill net sampling. Data recorded included specific conductivity, water temperature, salinity, pH, and dissolved oxygen (DO). Water quality data are provided in Table 2. ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA POST-UPRATE EVENT 7 DATA REPORT, ST. LUCIE PLANT EPU BIOLOGICAL STUDY Trawls were towed for 15 minutes along each of the nine transects. The scientific and common names of all specimens captured by all gear types are listed in Table 3. The numbers of fish and invertebrates collected in each 15-minute tow are presented in Table 4. Because of variations in tow speed, the distances the trawl traveled during a 15-minute tow varied (based on GPS readings). In order to compare abundances among stations in terms of catch per unit effort, the numbers collected per kilometer of bottom sampled were calculated and are presented in Table 5. The numbers of fish and invertebrates collected by gill net on each of the nine transects is given in Table 6. At each transect the gill net began to be retrieved 30 minutes after it was set. However, the total soak time (time from when the net first entered the water until the time it was completely removed from the water) varied among transects because of differences in retrieval times associated with variations in the number of fish present in the net. In order to compare abundances among stations in terms of catch per unit effort, the numbers collected per hour of total soak time were calculated and are given in Table 7. Beach seines were deployed at each of the nine stations previously described. The numbers of fish and invertebrates collected at each station are presented in Table 8. A maximum of 25 representative specimens of each Representative Important Species (RIS) of fish collected at each transect/station by trawl, gill net, and beach seine were measured (total length) and a batch weight for those specimens was obtained. Average lengths and average weights of each RIS at each station/transect for each gear type are presented in Table 9. Bongo nets were used to collect fish eggs and larvae as well as commercially or recreationally important invertebrate larvae at each of the six transects previously described. Nets were pulled for 15 minutes and the volume of water filtered determined by means of flow meters. Numbers of individuals per cubic meter of water filtered are given in Table 10. To quantify the number of sea turtles present in each of the three sampling sites, one one- kilometer-long transect in each sampling site was traversed by boat twice. The numbers of sea turtles sighted during each pass along each transect are presented in Table 11. ECOLOGICAL ASSOCIATES, INC., JENSEN BEACH, FLORIDA 2 Table 1. Environmental Data, Post-Uprate Sampling Event 7 (January 2014 - February 2014), St. Luice Plant EPU Biological Study. Values reflect the range of values recorded throughout each day of sampling. Wind Speed and Sampling Date Sea Conditions Air Temp Sky Conditions Direction Trawls/ 1/27/2014 0-2 ft swells 19.2-24.4°C 1-5 mph, NNW to SSW Clear to Partly Cloudy Ichthyoplankton Trawls/ 1/28/2014 0-2 ft swells 18.2-20.4°C 0-3 mph, W to SW Clear to Partly Cloudy Ichthyoplankton Gill Nets 1/27/2014 1-2 ft Swells 20.2-25.1°C 3-12 mph, S-SW Clear Gill Nets 1/28/2014 1 ft Swells 20.8-24.0°C 3-5 mph, SW Clear Beach Seines 2/25/2014 1-2 ft Swells 23.0-27.4°C 5-10 mph, N to NE Clear to Mostly Cloudy Sea Turtle 2/25/2014 2-3 ft Swells 24.4-25.4°C 5-10 mph, NE Partly Cloudy Transects Table 2. Water Quality Data, Post Uprate Sampling Event 7 (January 2014 - February 2014), St. Lucie Plant EPU Biological Study. Specific Water Salinity DO Transect Station Depth Conductivity Temp pH (PSU) (mg/l) (mS/cm) (°C) Surface 53.4 20.2 34.8 8.4 6.4 North Mid-Depth 53.3 20.0 34.9 8.4 6.4 Bottom 53.3 19.4 34.8 8.4 6.4 Surface 53.3 20.5 34.9 8.4 6.4 Trawl Middle Mid-Depth 53.3 20.1 34.8 8.4 6.4 SL1 A Bottom 53.3 19.5 34.8 8.4 6.4 Surface 53.4 20.4 34.9 8.4 6.4 South Mid-Depth 53.3 20.3 34.9 8.4 6.4 Bottom 53.3 19.8 34.9 8.4 6.4 Surface 53.4 21.1 35.0 8.4 6.4 North Mid-Depth 53.4 20.7 35.0 8.4 6.4 Bottom 53.5 19.2 35.0 8.4 6.6 Surface 53.4 21.2 35.0 8.4 6.2 Trawl Middle Mid-Depth 53.4 20.9 35.0 8.3 6.3 SL1 B Bottom 53.4 19.3 35.0 8.3 6.3 Surface 53.5 21.2 35.0 8.4 6.1 South Mid-Depth 53.5 21.0 35.0 8.4 6.2 Bottom 53.4 19.6 34.9 8.4 6.3 Surface 53.7 22.3 35.3 8.4 6.2 North Mid-Depth 53.5 22.2 35.2 8.4 6.2 Bottom 53.5 21.6 35.1 8.4 6.3 Surface 53.6 22.4 35.2 8.4 6.3 Trawl Middle Mid-Depth 53.5 22.3 35.2 8.4 6.2 SL1 C Bottom 53.5 22.1 35.2 8.4 6.3 Surface 53.5 22.6 35.2 8.4 6.3 South Mid-Depth 53.5 22.5 35.2 8.4 6.3 Bottom 53.5 22.1 35.2 8.4 6.3 Surface 53.3 18.8 34.8 8.3 6.7 North Mid-Depth 53.3 18.8 34.8 8.3 6.7 Bottom 53.3 18.8 34.8 8.3 6.7 Surface 53.3 18.7 34.9 8.3 6.6 Trawl Middle Mid-Depth 53.3 18.8 34.8 8.3 6.6 SL2 A Bottom 53.3 18.8 34.8 8.3 6.7 Surface 53.3 19.4 34.8 8.4 6.7 South Mid-Depth 53.3 19.1 34.8 8.4 6.7 Bottom 53.3 18.9 34.8 8.4 6.7 Specific Water Salinity DO Transect Station Depth Conductivity Temp pH (PSU) (mg/l) (mS/cm) (°C) Surface 53.6 23.1 35.2 8.3 6.4 North Mid-Depth 53.6 22.2 35.2 8.3 6.4 Bottom 53.5 22.0 35.1 8.3 6.4 Surface 53.6 23.1 36.3 8.4 6.3 Trawl Middle Mid-Depth 53.6 22.2 35.2 8.3 6.3 SL2 B Bottom 53.6 22.0 35.1 8.3 6.4 Surface 53.6 23.1 35.3 8.4 6.1 South Mid-Depth 53.6 22.2 35.1 8.4 6.3 Bottom 53.6 22.0 35.1 8.4 6.3 Surface 53.6 23.6 35.4 8.4 6.2 North Mid-Depth 53.6 22.2 35.2 8.4 6.2 Bottom 53.5 21.9 35.1 8.4 6.3 Surface 53.6 23.6 35.3 8.3 6.2 Trawl Middle Mid-Depth 53.5 22.1 35.2 8.3 6.3 SL2 C Bottom 53.5 21.9 35.1 8.3 6.4 Surface 53.6 23.6 35.3 8.3 6.2 South Mid-Depth 53.6 22.6 35.2 8.3 6.2 Bottom 53.5 21.9 35.2 8.3 6.4 Surface 53.1 21.4 34.8 8.3 6.5 North Mid-Depth 53.1 21.3 34.8 8.3 6.5 Bottom 53.1 21.1 34.8 8.3 6.5 Surface 53.1 21.4 34.8 8.3 6.5 Trawl Middle Mid-Depth 53.1 21.5 34.8 8.3 6.4 SL3 A Bottom 53.1 21.5 34.8 8.3 6.5 Surface 53.1 21.2 34.8 8.4 6.4 South Mid-Depth 53.1 21.2 34.8 8.4 6.4 Bottom 53.1 21.2 34.8 8.4 6.4 Surface 53.4 23.4 35.1 8.3 6.4 North Mid-Depth 53.4 23.3 35.1 8.3 6.4 Bottom 53.4 20.1 35.1 8.3 6.4 Surface 53.4 22.9 35.1 8.3 6.4 Trawl Middle Mid-Depth 53.4 23.0 35.1 8.3 6.4 SL3 B Bottom 53.4 20.1 35.1 8.3 6.3 Surface 53.6 23.1 35.2 8.3 6.3 South Mid-Depth 53.5 23.2 35.1 8.3 6.2 Bottom 53.5 20.1 35.1 8.3 6.3 Specific Water Salinity DO Transect Station Depth Conductivity Temp pH (PSU) (mg/l) (mS/cm) (°C) Surface 53.8 23.9 35.4 8.3 6.4 North Mid-Depth 53.7 23.7 35.4 8.3 6.4 Bottom 53.7 23.0 35.3 8.3 6.4 Surface 53.7 23.9 35.3 8.3 6.4 Trawl Middle Mid-Depth 53.7 23.7 35.3 8.3 6.4 SL3 C Bottom 53.6 22.5 35.2 8.3 6.5 Surface 53.6 23.9 35.3 8.3 6.4 South Mid-Depth 53.6 23.9 35.3 8.3 6.5 Bottom
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