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Data Sheet 163-0P Rudolf-Wissell-Str. 28a Background 37079 Göttingen, Germany Phone: +49 551-50556-0 Synaptopodin is the founding member of a new class of proline-rich actin-associated proteins. Up to Fax: +49 551-50556-384 now three splice variants of synaptopodin have been identified. In neurons and podocytes the protein E-mail: [email protected] is expressed in a differentiation dependent manner. Web: www.sysy.com Synaptopodin deficient mice lack the dendritic spine apparatus and exhibit impaired activity- Synaptopodin dependent long-term synaptic plasticity. Cat.No. 163-0P; control protein, 100 µg protein (lyophilized) Selected General References Synaptopodin and 4 novel genes identified in primary sensory neurons. Verpoorten N, Verhoeven K, Weckx S, Jacobs A, Serneels S, Del Favero J, Ceuterick C, Van Bockstaele DR, Berneman ZN, Data Sheet Van den Bosch L, Robberecht W, et al. Molecular and cellular neurosciences (2005) 303: 316-25. Postnatal development of synaptopodin expression in the rodent hippocampus. Reconstitution/ 100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml Storage solution in TBS. Then aliquot and store at -20°C to -80°C until use. Czarnecki K, Haas CA, Bas Orth C, Deller T, Frotscher M The Journal of comparative neurology (2005) 4902: 133-44. For detailed information, see back of the data sheet. Lamina-specific distribution of Synaptopodin, an actin-associated molecule essential for the spine apparatus, in identified Immunogen Recombinant protein corresponding to AA 331 to 452 from mouse Synaptopodin principal cell dendrites of the mouse hippocampus. (UniProt Id: Q8CC35-2) Bas Orth C, Vlachos A, Del Turco D, Burbach GJ, Haas CA, Mundel P, Feng G, Frotscher M, Deller T The Journal of comparative neurology (2005) 4873: 227-39. Recommended Optimal concentrations should be determined by the end-user. dilution Synaptopodin regulates the actin-bundling activity of alpha-actinin in an isoform-specific manner. Asanuma K, Kim K, Oh J, Giardino L, Chabanis S, Faul C, Reiser J, Mundel P Matching 163 002, 163 004 The Journal of clinical investigation (2005) 1155: 1188-98. antibodies Synaptopodin, a molecule involved in the formation of the dendritic spine apparatus, is a dual actin/alpha-actinin binding protein. Remarks This control protein consists of the recombinant protein (aa 331 - 452) that has Kremerskothen J, Plaas C, Kindler S, Frotscher M, Barnekow A been used for immunization. It has been tested in preadsorption experiments and Journal of neurochemistry (2005) 923: 597-606. blocks efficiently and specifically the corresponding signal in Western blots. The Synaptopodin-deficient mice lack a spine apparatus and show deficits in synaptic plasticity. amount of protein needed for efficient blocking depends on the titer and on the Deller T, Korte M, Chabanis S, Drakew A, Schwegler H, Stefani GG, Zuniga A, Schwarz K, Bonhoeffer T, Zeller R, Frotscher M, et affinity of the antibody to the antigen. al. Proceedings of the National Academy of Sciences of the United States of America (2003) 10018: 10494-9. Regulated expression of an actin-associated protein, synaptopodin, during long-term potentiation. TO BE USED IN VITRO / FOR RESEARCH ONLY Yamazaki M, Matsuo R, Fukazawa Y, Ozawa F, Inokuchi K NOT TOXIC, NOT HAZARDOUS, NOT INFECTIOUS, NOT CONTAGIOUS Journal of neurochemistry (2001) 791: 192-9. Expression of synaptopodin, an actin-associated protein, in the rat hippocampus after limbic epilepsy. Roth SU, Sommer C, Mundel P, Kiessling M Access the online factsheet including applicable protocols Brain pathology (Zurich, Switzerland) (2001) 112: 169-81. Potential role of synaptopodin in spine motility by coupling actin to the spine apparatus. at https://sysy.com/product/163-0P or scan the QR-code. Deller T, Mundel P, Frotscher M Hippocampus (2000) 105: 569-81. Actin-associated protein synaptopodin in the rat hippocampal formation: localization in the spine neck and close association with the spine apparatus of principal neurons. Deller T, Merten T, Roth SU, Mundel P, Frotscher M The Journal of comparative neurology (2000) 4182: 164-81. Synaptopodin: an actin-associated protein in telencephalic dendrites and renal podocytes. Mundel P, Heid HW, Mundel TM, Krüger M, Reiser J, Kriz W The Journal of cell biology (1997) 1391: 193-204. Podocytes in glomerulus of rat kidney express a characteristic 44 KD protein. Mundel P, Gilbert P, Kriz W The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1991) 398: 1047-56. FAQ - How should I store my antibody? Polyclonal Antibodies Crude antisera: With anti-microbials added, they may be stored at 4°C. However, frozen Shipping Conditions storage (-20°C up to -80°C) is preferable. Affinity purified antibodies: Less robust than antisera. Storage at -20°C up to -80°C is All our antibodies and control proteins / peptides are shipped lyophilized (vacuum freeze- recommended. Adding a carrier protein like BSA will increase long term stability. Most of our dried) and are stable in this form without loss of quality at ambient temperatures for antibodies already contain carrier proteins. Please refer to the data-sheet for detailed several weeks. information. Storage of Sealed Vials after Delivery Fluorescence-labeled Antibodies Unlabeled and biotin-labeled antibodies and control proteins should be stored at 4°C Store as a liquid with 1 : 1 (v/v) glycerol at -20°C. Protect these antibodies from light exposure. before reconstitution. They must not be stored in the freezer when still lyophilized! Temperatures below zero may cause loss of performance. Fluorescence-labeled antibodies should be reconstituted immediately upon receipt. Long Avoid repeated freeze-thaw cycles term storage (several months) may lead to aggregation. Control peptides should be kept at -20°C before reconstitution. for all antibodies! Long Term Storage after Reconstitution (General Considerations) The storage freezer must not be of the frost-free variety ("no-frost freezer"). This cycle between freezing and thawing (to reduce frost-build-up), which is exactly what should be FAQ - How should I reconstitute my antibody? avoided. For the same reason, antibody vials should be placed in an area of the freezer that has minimal temperature fluctuations, for instance towards the back rather than on a door Reconstitution shelf. Aliquot the antibody and store frozen (-20°C to -80°C). Avoid very small aliquots (below 20 µl) All our antibodies are lyophilized from PBS. To reconstitute the antibody in PBS, add the and use the smallest storage vial or tube possible. The smaller the aliquot, the more the stock amount of deionized water given in the respective datasheet. If higher volumes are preferred, concentration is affected by evaporation and adsorption of the antibody to the surface of the add water as mentioned above and then the desired amount of PBS and a stabilizing carrier storage vial or tube. Adsorption of the antibody to the surface leads to a substantial loss of protein (e.g. BSA) to a final concentration of 2%. Some of our antibodies already contain activity. albumin. Take this into account when adding more carrier protein. The addition of glycerol to a final concentration of 50% lowers the freezing point of your stock For complete reconstitution, carefully remove the lid. After adding water, briefly vortex the and keeps your antibody at -20°C in liquid state. This efficiently avoids freeze and thaw cycles. solution. You can spin down the liquid by placing the vial into a 50 ml centrifugation tube filled with paper. Product Specific Hints for Storage If desired, add small amounts of azide or thimerosal to prevent microbial growth. This is especially recommended if you want to keep an aliquot a 4°C. Control proteins / peptides After reconstitution of fluorescence-labeled antibodies, add 1 : 1 (v/v) glycerol to a final concentration of 50%. This lowers the freezing point of your stock and keeps your antibody in Store at -20°C to -80°C. liquid state at -20°C. Glycerol may also be added to unlabeled primary antibodies. It is a suitable way to avoid freeze- thaw cycles. Monoclonal Antibodies Please refer to our tips and hints for subsequent storage of reconstituted antibodies and control peptides and proteins. Ascites and hybridoma supernatant should be stored at -20°C up to -80°C. Prolonged storage at 4°C is not recommended! Unlike serum, ascites may contain proteases that will degrade the antibodies. Purified IgG should be stored at -20°C up to -80°C. Adding a carrier protein like BSA will increase long term stability. Many of our antibodies already contain carrier proteins. Please refer to the data-sheet for detailed information. Powered by TCPDF (www.tcpdf.org).
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