Bull Mar Sci. 94(1):47–71. 2018 research paper https://doi.org/10.5343/bms.2017.1060 Genetic population structure of the spotted seatrout (Cynoscion nebulosus): simultaneous examination of the mtDNA control region and microsatellite marker results 1 Florida Fish and Wildlife Seifu Seyoum 1 * Conservation Commission, Fish 2 and Wildlife Research Institute, Richard S McBride 1 100 Eighth Avenue Southeast, Michael D Tringali St. Petersburg, Florida 33701. Vicki L Villanova 3 2 National Marine Fisheries Cecilia Puchutulegui 1 Service, Northeast Fisheries 1 Science. Center, 166 Water Samantha Gray Street, Woods Hole, Nathan Van Bibber 1 Massachusetts 02543. 3 Harris County Institute of Forensic Sciences, Forensic Genetics Laboratory, 2450 ABSTRACT.—The spotted seatrout, Cynoscion nebulosus Holcombe Boulevard, Suite 7 (Cuvier, 1830), lives almost entirely within natal estuaries, and Houston, Texas 77021. although many genetic studies identify fine-scale population structure, they do not provide a consensus as to the number * Corresponding author email: <
[email protected]>. of stocks, the stock boundaries, or their connectivity. Two perceived limitations of previous studies were addressed. To address a presumption of limited sampling, we assembled a large sample size with broad geographic range: 547 specimens from 18 sampling areas (mean inter-area distance = 270 km) between Texas and North Carolina, representing the majority of this species’ range. To address the presumptive limitations of genetic markers, two genetic marker types were compared: a 335-base-pair segment of the mitochondrial DNA (mtDNA) control region and 38 microsatellite loci. MtDNA haplotype frequencies were different only between populations of the Gulf of Mexico (GoM) and the Atlantic Ocean, but not between the western GoM and the eastern GoM.