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Supporting Information Supporting Information Cusimano et al. 10.1073/pnas.0810243106 SI Text slides (Nalge Nunc International). Two days after plating, cells Immunofluorescence and Antibodies. For immunofluorescence, were transiently transfected by the lipofectamine-Plus method 9 ϫ 104 primary rat myoblasts/cm2 were plated on 2% gelatin- (Invitrogen) and induced to differentiate in alpha-minimum ␣ coated (Sigma) glass coverslips and differentiated as described essential medium ( -MEM; Bio-Whittaker) containing 2 mM ␮ previously (1). Primary skeletal muscle cells were fixed at room L-glutamine (Bio-Whittaker), 100 g/mL streptomycin (Bio- ␮ temperature with 3% paraformaldehyde and 2% sucrose in PBS, Whittaker), 100 U/mL penicillin (Bio-Whittaker), 0,1 M dexa- ␮ permeabilized in Hepes Triton Buffer (20 mM Hepes, pH 7.4, methasone (Sigma), and 50 M hydrocortisone (Sigma), sup- plemented with 10% heat-inactivated FBS (Sigma) and 5% 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, and 0.5% Triton X-100) and blocked in PBS containing 0.2% BSA and 5% goat heat-inactivated horse serum (Biochrom). NIH 3T3 cells were ϫ 4 2 serum. Cells were incubated overnight with the primary anti- plated at 6 10 /cm per LabTek chamber slide and transiently bodies. Rabbit polyclonal antibodies included anti-ank1.5 (1), transfected the day after. FRAP experiments were performed by anti-RyR1 (2), and anti-triadin (trisk-95), kindly provided by I. using a Zeiss LSM 510 Meta confocal microscope. Cells were Marty (Universite´ Joseph Fouriere, Grenoble, France). Mouse imaged in LabTek chambers in medium containing 140 mM ␣ NaCl, 5 mM KCl, 10 mM glucose, 1 mM MgCl2, 0.1 mM CaCl2, monoclonal antibodies included anti -actinin (A-7811; clone ϫ EA-53; Sigma), anti-RyR (34C; Alexis), and anti-SERCA1 20 mM Hepes, and 0.4 mM EGTA. A 63 1.4 N.A. Plan- (CaF2–5D2; Developmental Studies Hybridoma Bank). Second- Apochromat oil immersion objective (Zeiss) was used with a pinhole aperture of 4.96 Airy units. GFP was excited at 488 nm ary goat anti-rabbit and goat anti-mouse IgG antibodies, con- with an argon laser with low laser power (0.5%) and emitted jugated with CyTM2orCyTM3, were purchased from Jackson fluorescence collected with a long-pass 505 emission filter. After ImmunoResearch Laboratories. Images were obtained with a acquisition of 10 prebleach images, photobleaching of GFP in a Zeiss LSM 510 Meta confocal microscope. circular area 1.08 ␮m in diameter was performed by using the argon laser lines 458, 477, and 488 nm at 50% laser power and GFP Fusion Proteins. Full-length cDNA of human ank1.5 (3) was 50% transmission. Recovery of fluorescence in the bleached inserted into the EcoRI/BamHI sites of the pEGFP-N1 vector ⌬ region was recorded every 50 ms over a period of 1–10 min until (Clontech). The ank1.5 -GFP mutant lacking the binding site fluorescence level reached a plateau. Fluorescence intensities for obscurin has been described previously (3). The construct were acquired for the bleached region (I ), the whole cell pSG5-SERCA2a was kindly provided by E. Carafoli (University frap (Iwhole), and a background region (Ibase). The data were low-pass of Padova, Padova, Italy) and subcloned into the EcoRI site of 3 ϫ 3 filtered with the Zeiss LSM 510 software for image noise the pEGFP-C3 expression vector. Full-length cDNA of murine reduction, and data analysis was performed by using macros InsP3R1 was kindly provided by K. Mikoshiba (University of designed in IgorPro software (WaveMetrics Inc.). The average Tokyo, Tokyo, Japan). The coding sequence of GFP was am- fluorescence intensity within the bleached region was normal- plified and inserted into the Nhe/AflII sites of pcDNA3.1- ized to prebleach intensity for each time point and corrected for ⌬ InsP3R1. The GFP-InsP3R1 14 mutant was prepared as de- loss of fluorescence during acquisition. Fluorescence loss during scribed previously (4). Rabbit RyR1 cDNA was kindly provided acquisition was basically no more than 10%. The normalized by P. D. Allen (Brigham and Women’s Hospital, Boston, MA) data were fitted by the exponential equation: I frap-est(t) ϭ A and cloned into the pEGFP-C3 vector. Full-length triadin (95- Ϫ␶ t (1-e 1 ) (1), where A represents the mobile fraction. Fitting kDa isoform) and junctin were amplified by PCR from a human quality was evaluated by the probability Q value and accepted cDNA library and cloned into the pEGFP-N3 vector. Human when Q Ͼ 0.01. The diffusion constant was calculated by using full-length JP1 was amplified by PCR and cloned in pEGFP-C2. the Soumpasis diffusion fitting (7). Statistical analyses were To obtain the triadin-junctin-GFP chimera, the fragment from performed by using Instat Software (GraphPad). Data were amino acids 1 to 73 of triadin and the fragment from amino acids initially evaluated for normal distribution with the Kolmogorov– 51 to 625 of junctin were amplified by PCR. The fragments Smirnov test. Not normally distributed data were compared by obtained were joined by PCR and cloned into the EcoRI/BamHI Mann–Whitney test. Comparisons between 2 groups with nor- sites of pEGFP-N3. The chimera junctin-triadin-GFP was ob- mally distributed variables were analyzed by Student’s unpaired tained by amplification of the fragments from amino acids 1 to t-test. Comparisons between 3 groups were analyzed by 1-way 50 of junctin and from amino acids 73 to 728 of triadin. The ANOVA followed by Bonferroni’s posthoc. Data are presented fragments were joined by PCR and cloned into the EcoRI/ as mean Ϯ SD. BamHI sites of pEGFP-N3. The constructs pEGFPC-␣1S (5) and pcDNA-␤1A (6), coding for subunits of rabbit DHPR, were Qualitative iFRAP Experiments. For iFRAP experiments, a 63 ϫ 1.4 kindly provided by M. Grabner and B. E. Flucher (Innsbruck N.A. Plan-Apochromat oil immersion objective and a pinhole Medical University, Innsbruck, Austria). To facilitate expression aperture of 4.96 Airy units were used. Considering that the and surface localization of heterologous DHPR GFP-␣1S, myo- myotubes were too long to be entirely visualized, a region of a blasts were cotransfected with the untagged ␤1A subunit of transfected myotube was selected for the bleaching. After ac- DHPR. All constructs were sequence-verified. quisition of 3 prebleach images, fluorescence was bleached by using full laser power, leaving unbleached a small area in the FRAP Experiments and Statistical Analysis. For FRAP analyses, 6 ϫ center of the myotube region. Postbleach images were acquired 104 cells/cm2 were plated in 2% gelatin-coated LabTek chamber- for up to 20 min at 300-ms intervals. 1. Armani A, et al. (2006) Molecular interactions with obscurin are involved in the 2. Giannini G, Conti A, Mammarella S, Scrobogna M, Sorrentino V (1995) The ryanodine localization of muscle-specific small ankyrin1 isoforms to subcompartments of the receptor/calcium channel genes are widely and differentially expressed in murine brain sarcoplasmic reticulum. Exp Cell Res 312:3546–3558. and peripheral tissues. J Cell Biol 128:893–904. Cusimano et al. www.pnas.org/cgi/content/short/0810243106 1of9 3. Bagnato P, Barone V, Giacomello E, Rossi D, Sorrentino V (2003) Binding of an 7. Soumpasis DM (1983) Theoretical analysis of fluorescence photobleaching recovery Ankyrin-1 isoform to Obscurin suggests a molecular link between the sarcoplasmic experiments. Biophys J 41:95–97. reticulum and myofibrils in striated muscles. J Cell Biol 160:245–253. 8. Phimister AJ, et al. (2007) Conformation-dependent stability of junctophilin 1 (JP1) and 4. Zhang S, et al. (2003) Protein 4.1N is required for translocation of inositol 1,4,5- ryanodine receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive triphosphate receptor type 1 to the basolateral membrane domain in polarized thiols. J Biol Chem 282:8667–8677. Madin-Darby canine kidney cells. J Biol Chem 278:4048–4056. 9. Kontrogianni-Konstantopoulos A, et al. (2006) Obscurin modulates the assembly and 5. Grabner M, Dirkens RT, Beam KG (1998) Tagging with green fluorescent protein reveals organization of sarcomeres and the sarcoplasmic reticulum. FASEB J 20:2102–2111. a distinct subcellular distribution of L-type and non-L-type Ca2ϩ channels expressed in 10. Fukatsu K, et al. (2004) Lateral diffusion of inositol 1,4,5-trisphosphate receptor type dysgenic myotubes. Proc Natl Acad Sci USA 95:1903–1908. 1 is regulated by actin filaments and 4.1N in neuronal dendrites. J Biol Chem 6. Schredelseker J, et al. (2005) The beta 1a subunit is essential for the assembly of 279:48976–48982. dihydropyridine-receptor arrays in skeletal muscle. Proc Natl Acad Sci USA 102:17219– 11. Fukatsu K, Bannai H, Inoue T, Mikoshiba K (2006) 4.1N binding of inositol 1,4,5- 17224. triphosphate receptor typ1 1. Biochem Biophys Res Commun 342:573–576. Cusimano et al. www.pnas.org/cgi/content/short/0810243106 2of9 Fig. S1. Localization of ank1.5, SERCA and RyR in differentiating myotubes. In a fraction of 4-day differentiating myotubes, in addition to the ank1.5-signal at the level of the M-band, a less intense signal could be observed at the level of the Z-disk (A, C). Staining with ␣-actinin is shown to identify the Z-disk, (B, C). In a fraction of 6-day differentiating myotubes, ank1.5 could still be observed only at the level of the M-band (D, F), as shown by the alternating pattern with ␣-actinin (E, F). In 6-day differentiated myotubes, RyR signal was organized at triads (G, I) only in cells where SERCA staining (H, I) was organized at the Z-disk. (Scale bar, 5␮m.) Cusimano et al.
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