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The Structure/Function of New Insecticidal Proteins and Regulatory Challenges for Commercialization
This is an Open Access document downloaded from ORCA, Cardiff University's institutional repository: http://orca.cf.ac.uk/100278/ This is the author’s version of a work that was submitted to / accepted for publication. Citation for final published version: Moar, William, Berry, Colin and Narva, Kenneth 2017. The structure/function of new insecticidal proteins and regulatory challenges for commercialization. Journal of Invertebrate Pathology 142 , pp. 1-4. 10.1016/j.jip.2017.02.001 file Publishers page: https://doi.org/10.1016/j.jip.2017.02.001 <https://doi.org/10.1016/j.jip.2017.02.001> Please note: Changes made as a result of publishing processes such as copy-editing, formatting and page numbers may not be reflected in this version. For the definitive version of this publication, please refer to the published source. You are advised to consult the publisher’s version if you wish to cite this paper. This version is being made available in accordance with publisher policies. See http://orca.cf.ac.uk/policies.html for usage policies. Copyright and moral rights for publications made available in ORCA are retained by the copyright holders. The structure/function of new insecticidal proteins and regulatory challenges for commercialization William Moar, Colin Berry and Kenneth Narva Genetically modified crops produced by biotechnology methods have provided grower benefits since 1995 including improved protection of crop yield, reduced input costs, and a reduced reliance on chemical pesticides (Klumper and Qaim, 2014). These benefits have driven annual increases in worldwide adoption of GM crops, with the largest number of hectares being grown in the Americas (ISAAA 2014). -
Disaster Preparedness Level, Graph Showed the Data in %, Developed on the Basis of Survey Conducted in Region Vi
2014 Figures Nature Begins Where Human Predication Ends Typhoon Frank (Fengshen) 17th to 27th June, 2008 Credit: National Institute of Geological Sciences, University of the Philippines, 2012 Tashfeen Siddique – Research Fellow AIM – Stephen Zuellig Graduate School of Development Management 8/15/2014 Nature Begins Where Human Predication Ends Contents Acronyms and Abbreviations: ...................................................................................................... iv Brief History ........................................................................................................................................ 1 Philippines Climate ........................................................................................................................... 2 Chronology of Typhoon Frank ....................................................................................................... 3 Forecasting went wrong .................................................................................................................. 7 Warning and Precautionary Measures ...................................................................................... 12 Typhoon Climatology-Science ..................................................................................................... 14 How Typhoon Formed? .............................................................................................................. 14 Typhoon Structure ..................................................................................................................... -
US Department of Agriculture
Environmental Assessment for Dow/Pioneer Rootworm Resistant Corn APHIS’ Analysis and Response to Comments Received on Petition 03-353-01p and the EA. A total of two comments were submitted during the designated 60-day comment period by a private individual and a national trade association. The comment submitted by the trade association supported deregulation of the Cry34/35 Ab1 corn rootworm resistant corn (CRW). This comment stressed the importance of a different mechanism of control compared to the only CRW resistant corn product on the market while providing a new option for managing insect resistance. The commenter also mentioned that this product would provide a wider range of plant protection. The comment in opposition to deregulation of this product was submitted by a private citizen. This commenter expressed a general disapproval of genetically modified plants and suggested that this product should always be regulated. APHIS disagrees with this comment. The author fails to provide any information to support her assertion that this petition should be denied. The commenter also suggested that APHIS extend the comment period, but failed to provide a reason for the extension. Further, this commenter discussed pesticidal issues related to EPA’s role in the regulation of plant incorporated protectants. APHIS defers to EPA on issues related to EPA’s authority. - 2 - Environmental Assessment for Dow/Pioneer Rootworm Resistant Corn USDA/APHIS Decision on Dow AgroSciences and Pioneer Hi-Bred International Petition 03-353-01P Seeking a Determination of Nonregulated Status for Bt cry34/35Ab1 Insect Resistant Corn Line DAS-59122-7 Environmental Assessment TABLE OF CONTENTS I. -
World Bank Document
Report No. 105363-PH Public Disclosure Authorized ENHANCING COMPETITION CONDITIONS AND COMPETITIVENESS OF PHILIPPINE DOMESTIC SHIPPING September 2014 Public Disclosure Authorized The World Bank and International Finance Corporation Philippine Country Office East Asia and Pacific Region Public Disclosure Authorized A project of the World Bank, with the support of the Australian Government through the Australia-World Bank Philippines Development Trust Fund Public Disclosure Authorized Document of the World Bank Group 1 Enhancing competition conditions and competitiveness of Philippine domestic shipping services1 Contents Executive Summary ..................................................................................................................................... 4 The need for clarity and simplicity ...................................................................................................... 5 Direction forward ................................................................................................................................ 6 Creating a level playing field ............................................................................................................... 7 Facilitating greater competition between operators ......................................................................... 8 More efficient and cost effective logistics .............................................................................................. 9 Promoting better decisions .............................................................................................................. -
Bacillus Thuringiensis As a Specific, Safe, and Effective Tool for Insect Pest Control
J. Microbiol. Biotechnol. (2007), 17(4), 547–559 Bacillus thuringiensis as a Specific, Safe, and Effective Tool for Insect Pest Control ROH, JONG YUL, JAE YOUNG CHOI, MING SHUN LI, BYUNG RAE JIN1, AND YEON HO JE* Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea 1College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea Received: November 21, 2006 Accepted: January 2, 2007 Bacillus thuringiensis (Bt) was first described by Berliner The insecticidal bacterium Bt is a Gram-positive [10] when he isolated a Bacillus species from the Mediterranean bacterium that produces proteinaceous inclusions during flour moth, Anagasta kuehniella, and named it after the sporulation [53]. These inclusions can be distinguished as province Thuringia in Germany where the infected moth distinctively shaped crystals by phase-contrast microscopy. was found. Although this was the first description under The inclusions are composed of proteins known as crystal the name B. thuringiensis, it was not the first isolation. In proteins, Cry proteins, or δ-endotoxins, which are highly 1901, a Japanese biologist, Ishiwata Shigetane, discovered toxic to a wide variety of important agricultural and a previously undescribed bacterium as the causative health-related insect pests as well as other invertebrates. agent of a disease afflicting silkworms. Bt was originally Because of their high specificity and their safety for the considered a risk for silkworm rearing but it has become environment, crystal proteins are a valuable alternative the heart of microbial insect control. The earliest commercial to chemical pesticides for control of insect pests in production began in France in 1938, under the name agriculture and forestry and in the home. -
Typhoon Fengshen
Emergency appeal n° MDRPH004 Philippines: GLIDE n° TC-2008-000093-PHL Operations update n° 3 24 September 2008 Typhoon Fengshen Period covered by this Operations Update: 28 July – 12 September 2008 Appeal target: CHF 8,310,213 (USD 8 million or EUR 5.1 million) Appeal coverage: To date, the appeal is 19 per cent1 covered. Funds are urgently needed to enable the Philippines National Red Cross to provide assistance to those affected by the typhoon. <click here to go directly to the interim financial report, or here to link to contact details > Appeal history: • A preliminary emergency appeal was launched on 24 June 2008 for CHF 8,310,213 (USD 8 million or EUR 5.1 million) for twelve months to assist 6,000 families. • Disaster Relief Emergency Fund (DREF): CHF 200,000 was allocated from the International Federation’s DREF. Dedicated volunteers enable the Philippine National Red Cross, with further support from the Red Cross Red Crescent Movement, to reach the most vulnerable communities with essential support, including water and shelter. 1 Including soft pledges; please note that interim financial report reflects income up to end August 2008. Click here to access the live donors’ response list. Philippines: Typhoon Fengshen (MDRPH004), Operations Update no. 3 2 Summary: The onslaught of typhoon Fengshen which hit the Philippines on 18 June 2008, followed by floods and landslides, has left in its wake urgent needs among poverty-stricken communities. According to the National Disaster Coordinating Council (NDCC), approximately four million people have been affected through out the country by typhoon Fengshen. -
The Pesticidal Cry6aa Toxin from Bacillus Thuringiensis Is Structurally
Dementiev et al. BMC Biology (2016) 14:71 DOI 10.1186/s12915-016-0295-9 RESEARCH ARTICLE Open Access The pesticidal Cry6Aa toxin from Bacillus thuringiensis is structurally similar to HlyE- family alpha pore-forming toxins Alexey Dementiev1, Jason Board2, Anand Sitaram3, Timothy Hey4,7, Matthew S. Kelker4,10, Xiaoping Xu4, Yan Hu3, Cristian Vidal-Quist2,8, Vimbai Chikwana4, Samantha Griffin4, David McCaskill4, Nick X. Wang4, Shao-Ching Hung5, Michael K. Chan6, Marianne M. Lee6, Jessica Hughes2,9, Alice Wegener2, Raffi V. Aroian3, Kenneth E. Narva4 and Colin Berry2* Abstract Background: The Cry6 family of proteins from Bacillus thuringiensis represents a group of powerful toxins with great potential for use in the control of coleopteran insects and of nematode parasites of importance to agriculture. These proteins are unrelated to other insecticidal toxins at the level of their primary sequences and the structure and function of these proteins has been poorly studied to date. This has inhibited our understanding of these toxins and their mode of action, along with our ability to manipulate the proteins to alter their activity to our advantage. To increase our understanding of their mode of action and to facilitate further development of these proteins we have determined the structure of Cry6Aa in protoxin and trypsin-activated forms and demonstrated a pore-forming mechanism of action. Results: The two forms of the toxin were resolved to 2.7 Å and 2.0 Å respectively and showed very similar structures. Cry6Aa shows structural homology to a known class of pore-forming toxins including hemolysin E from Escherichia coli and two Bacillus cereus proteins: the hemolytic toxin HblB and the NheA component of the non- hemolytic toxin (pfam05791). -
Molecular Mode of Action of Cry6aa1, a New Insecticidal Bacillus Thuringiensis Toxin
i ii Université de Montréal Molecular mode of action of Cry6Aa1, a new insecticidal Bacillus thuringiensis toxin par Eva Fortea Verdejo Département de physiologie moléculaire et intégrative Faculté de médecine Mémoire présenté en vue de l’obtention du grade de Maîtrise en physiologie moléculaire, cellulaire et intégrative option physiologie et biophysique moléculaires Membres du jurée Lucie Parent, Réjean Couture et Jean-Louis Schwartz Août 2016 © Eva Fortea Verdejo, 2016 iii ABSTRACT Cry6Aa1 is a new toxin produced by Bacillus thuringiensis (Bt), which displays insecticidal activity against the Western corn rootworm (WCRW). The present work demonstrates that Cry6Aa1 is a pore-forming toxin (PFT) in planar lipid bilayers (PLBs). Contrary to other Bt toxins tested so far, pore formation by Cry6Aa1 does not require protease pretreatment and takes place at doses that are two to three orders of magnitude lower than those required for other Bt toxins under similar conditions. Pore formation by Cry6Aa1 is pH-dependent; the conductances of the pores range between 31 and 689 pS under symmetrical 150 mM KCl conditions; they are cationic and display a complex kinetic behaviour. The treatment of the toxin with midgut juice (Cry6Aa1 WCR1) does not change the biophysical properties of the pores. However, the treatment with trypsin (Cry6Aa1 TT) affects their conductance and selectivity at pH 5.5 (the WCRW gut physiological pH). The incorporation in PLBs of native membrane material from WCRW midgut affects the behaviour of the Cry6Aa1 pores. The molecular determinants of the mode of action of this new PFT appear therefore to differ from those reported before for other Bt toxins. -
Us 2018 / 0362598 A1
US 20180362598A1 ( 19) United States ( 12) Patent Application Publication (10 ) Pub . No. : US 2018 /0362598 A1 CARLSON et al. ( 43 ) Pub . Date: Dec . 20 , 2018 ( 54 ) CLEAVABLE PEPTIDES AND INSECTICIDAL CO7K 5 / 103 ( 2006 .01 ) AND NEMATICIDAL PROTEINS CO7K 14 /435 (2006 .01 ) COMPRISING SAME CO7K 14 /325 ( 2006 .01 ) (71 ) Applicant: VESTARON CORPORATION , A01N 63 /02 ( 2006 .01 ) KALAMAZOO , MI (US ) C12N 15 /82 (2006 .01 ) (72 ) Inventors : ALVAR R . CARLSON , (52 ) U . S . CI. KALAMAZOO , MI (US ) ; CPC . .. .. CO7K 14 /415 (2013 .01 ) ; CO7K 7 / 06 ALEXANDRA M . HAASE , MARTIN , (2013 .01 ) ; CO7K 5 / 101 ( 2013. 01 ) ; CO7K MI (US ) ; ROBERT M . KENNEDY , 14 / 43518 (2013 .01 ) ; CO7K 14 / 325 (2013 . 01 ) ; DEXTER , MI (US ) CO7K 2319 / 04 ( 2013 .01 ) ; C12N 15 /8285 ( 2013 . 01 ) ; C12N 15 /8286 ( 2013 .01 ) ; COZK ( 73 ) Assignee : VESTARON CORPORATION , 2319 /50 (2013 .01 ) ; CO7K 2319 /55 (2013 .01 ) ; KALAMAZOO , MI (US ) A01N 63 / 02 (2013 .01 ) ( 21 ) Appl. No. : 15 /727 , 277 ( 22 ) Filed : Oct . 6 , 2017 (57 ) ABSTRACT Related U . S . Application Data A peptide comprised of either a binary or a tertiary peptide, (60 ) Provisional application No. 62 /411 , 117 , filed on Oct. the peptide contains at least 4 amino acids and up to a 21 , 2016 . maximum of 16 amino acids, comprised of 2 or 3 different regions, wherein the binary peptides have 2 different regions Publication Classification and the tertiary peptides have 3 different regions; wherein , (51 ) Int . Cl. the peptide can be cleaved by both an animal gut protease CO7K 14 /415 ( 2006 .01 ) and an insect or nematode gut protease . -
Technical Document for Bacillus Thuringiensis Cry34ab1 And
BIOPESTICIDES REGISTRATION ACTION DOCUMENT Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 Proteins and the Genetic Material Necessary for Their Production (PHP17662 T-DNA) in Event DAS-59122-7 Corn (OECD Unique Identifier: DAS-59122-7) PC Code: 006490 U.S. Environmental Protection Agency Office of Pesticide Programs Biopesticides and Pollution Prevention Division Bacillus thuringiensis Cry34/35Ab1 Corn Biopesticides Registration Action Document (BRAD) September 2010 Table of Contents I. OVERVIEW A. Background 4 B. Use Profile 7 C. Regulatory History 8 II. SCIENCE ASSESSMENT A. Product Characterization 14 B. Human Health Assessment 41 C. Environmental Assessment 66 D. Insect Resistance Management 117 E. Benefits 172 III. REGULATORY POSITION FOR EVENT DAS-59122-7 CORN A. Initial Registration (August 31, 2005) 193 B. 2010 Update 195 C. Period of Registration 197 APPENDIX A 199 2 Bacillus thuringiensis Cry34/35Ab1 Corn Biopesticides Registration Action Document (BRAD) September 2010 Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 Proteins and the Genetic Material Necessary for Their Production (PHP17662 T-DNA) in Event DAS-59122-7 Corn Regulatory Action Team Product Characterization and Human Health Rebecca Edelstein, Ph.D. John Kough, Ph.D. Annabel Waggoner Chris Wozniak, Ph.D. Environmental Fate and Effects Joel Gagliardi, Ph.D. Hillary Hill Tessa Milofsky Robyn Rose Zigfridas Vaituzis, Ph.D. Annabel Waggoner Insect Resistance Management Jeannette Martinez Sharlene Matten, Ph.D. Tessa Milofsky Alan Reynolds Benefits Assessment Edward Brandt Sharlene Matten, Ph.D. Tessa Milofsky Registration Support Mike Mendelsohn Sheryl Reilly, Ph.D. Ann Sibold Biopesticides Registration Action Document Team Leaders Jeannine Kausch Mike Mendelsohn Office of General Counsel Angela Huskey, Esq. 3 Bacillus thuringiensis Cry34/35Ab1 Corn Biopesticides Registration Action Document (BRAD) September 2010 I. -
Biopesticides Fact Sheet for Bacillus Thuringiensis Cry34ab1 And
Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins and the genetic material necessary for their production (plasmid insert PHP 17662) in Event DAS-59122-7 corn (006490) Fact Sheet I. Description of the Plant-Incorporated Protectant o Pesticide Name: Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins and the genetic material necessary for their production (plasmid insert PHP 17662) in Event DAS-59122-7 corn o Date Registered: August 31, 2005 o Registration Numbers: 68467-5, 29964-4 o Trade and Other Names: Event DAS-59122-7 corn, Herculex Rootworm, Herculex RW o OPP Chemical Code: 006490 o Basic Manufacturers: Mycogen Seeds c/o Dow AgroSciences LLC 330 Zionsville Road, Indianapolis, IN 46268 Pioneer Hi-Bred International, A Dupont Company 7250 N.W. 62nd Ave., P.O. Box 552, Johnston, IA o Type of Pesticide: Plant-Incorporated Protectant o Uses: Field Corn o Target Pest(s): Corn Rootworm II. Background EPA has conditionally registered Mycogen Seeds c/o Dow AgroSciences LLC and Pioneer Hi-Bred International’s new active ingredient, Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins and the genetic material necessary for their production (plasmid insert PHP 17662) in Event DAS-59122- 7 corn. The Agency has determined that the use of this pesticide is in the public interest and that it will not cause any unreasonable adverse effects on the environment during the time of conditional registration. The new products are the second PIP to offer protection against corn rootworm (CRW), and they are expected to result in a further reduction of chemical insecticide use by growers. The reduced chemical pesticide use will benefit the environment directly and can mean less exposure to people who apply chemical pesticides to corn. -
USINDOPACOM Foreign Disaster Response in the Indo-Asia-Pacific June 1991 – June 2019
CENTER FOR EXCELLENCE IN DISASTER MANAGEMENT & HUMANITARIAN ASSISTANCE USINDOPACOM Foreign Disaster Response in the Indo-Asia-Pacific June 1991 – June 2019 WWW.CFE-DMHA.ORG Contents About the Center for Excellence in Disaster Management and Humanitarian Assistance ...3 Introduction ...........................................................................................................................4 June 1991 – Philippines: Mount Pinatubo Eruption ..............................................................6 April 1991 – Bangladesh: Cyclone Gorky (Marian) ............................................................10 January 2001 – India: Gujarat Earthquake .........................................................................16 December 2004 – Indian Ocean Earthquake and Tsunami ................................................18 October 2005 – Pakistan: Kashmir Earthquake .................................................................25 February 2006 – Philippines: Leyte Landslide ...................................................................29 May 2006 – Indonesia: Yogyakarta Earthquake .................................................................33 April 2007 – Solomon Islands: Tsunami .............................................................................37 November 2007 – Bangladesh: Cyclone Sidr.....................................................................39 May 2007 – Myanmar: Cyclone Nargis ..............................................................................43 May 2008 – China: Sichuan