Bacillus Thuringiensis As a Specific, Safe, and Effective Tool for Insect Pest Control
J. Microbiol. Biotechnol. (2007), 17(4), 547–559 Bacillus thuringiensis as a Specific, Safe, and Effective Tool for Insect Pest Control ROH, JONG YUL, JAE YOUNG CHOI, MING SHUN LI, BYUNG RAE JIN1, AND YEON HO JE* Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea 1College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea Received: November 21, 2006 Accepted: January 2, 2007 Bacillus thuringiensis (Bt) was first described by Berliner The insecticidal bacterium Bt is a Gram-positive [10] when he isolated a Bacillus species from the Mediterranean bacterium that produces proteinaceous inclusions during flour moth, Anagasta kuehniella, and named it after the sporulation [53]. These inclusions can be distinguished as province Thuringia in Germany where the infected moth distinctively shaped crystals by phase-contrast microscopy. was found. Although this was the first description under The inclusions are composed of proteins known as crystal the name B. thuringiensis, it was not the first isolation. In proteins, Cry proteins, or δ-endotoxins, which are highly 1901, a Japanese biologist, Ishiwata Shigetane, discovered toxic to a wide variety of important agricultural and a previously undescribed bacterium as the causative health-related insect pests as well as other invertebrates. agent of a disease afflicting silkworms. Bt was originally Because of their high specificity and their safety for the considered a risk for silkworm rearing but it has become environment, crystal proteins are a valuable alternative the heart of microbial insect control. The earliest commercial to chemical pesticides for control of insect pests in production began in France in 1938, under the name agriculture and forestry and in the home.
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