Microbial Food & Feed Ingredients
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In Vivo Studien Zur Anreicherung, Lokalisation Und Persistenz Von Vibrio Spp
Aus dem Institut der Lebensmittelhygiene des Fachbereichs Veterinärmedizin der Freien Universität Berlin „In vivo Studien zur Anreicherung, Lokalisation und Persistenz von Vibrio spp. in artifiziell kontaminierten Mytilus edulis“ Inaugural-Dissertation zur Erlangung des Grades eines Doktors der Veterinärmedizin an der Freien Universität Berlin vorgelegt von Doreen Herrfurth Tierärztin aus Belzig Berlin 2013 Journal-Nr.: 3652 Gedruckt mit Genehmigung des Fachbereichs Veterinärmedizin der Freien Universität Berlin Dekan: Univ.-Prof. Dr. Jürgen Zentek Erster Gutachter: Univ.-Prof. Dr. Thomas Alter Zweiter Gutachter: Prof. Dr. Reimar Johne Dritter Gutachter: PD Dr. Peggy Braun Deskriptoren (nach CAB-Thesaurus): Vibrio parahaemolyticus, Vibrio cholerae, Vibrio vulnificus, Mytilus edulis, contamination, concentration, localization, persistence Tag der Promotion: 13.11.2013 Bibliografische Information der Deutschen Nationalbibliothek Die Deutsche Nationalbibliothek verzeichnet diese Publikation in der Deutschen Nationalbibliografie; detaillierte bibliografische Daten sind im Internet über <http://dnb.ddb.de> abrufbar. ISBN: 978-3-86387-401-8 Zugl.: Berlin, Freie Univ., Diss., 2013 Dissertation, Freie Universität Berlin D 188 Dieses Werk ist urheberrechtlich geschützt. Alle Rechte, auch die der Übersetzung, des Nachdruckes und der Vervielfältigung des Buches, oder Teilen daraus, vorbehalten. Kein Teil des Werkes darf ohne schriftliche Genehmigung des Verlages in irgendeiner Form reproduziert oder unter Verwendung elektronischer Systeme verarbeitet, vervielfältigt oder verbreitet werden. Die Wiedergabe von Gebrauchsnamen, Warenbezeichnungen, usw. in diesem Werk berechtigt auch ohne besondere Kennzeichnung nicht zu der Annahme, dass solche Namen im Sinne der Warenzeichen- und Markenschutz-Gesetzgebung als frei zu betrachten wären und daher von jedermann benutzt werden dürfen. This document is protected by copyright law. No part of this document may be reproduced in any form by any means without prior written authorization of the publisher. -
Sampling and Quantification of Biofilms in Food Processing And
21 Sampling and quantifi cation of biofi lms in food processing and other environments D. E. Nivens, and B. M. Co, Purdue University, USA and M. J. Franklin, The Center for Biofi lm Engineering, USA Abstract: In the food industry, assessment of food contact surfaces is necessary to determine whether equipment is properly cleaned and/or sanitized and whether living problematic microorganisms are present. Existing quantitative detection technologies are limited by the inability to directly detect living cells in sporadically dispersed biofi lms on large surface areas. Thus, precise and accurate sampling strategies must be coupled with detection technology. This chapter discusses sampling methods and standard (e.g., plating and ATP-bioluminescence) and emerging (e.g., spectrometry, immunosensor, and nucleic acid-based) quantitative techniques to detect biofi lms on food contact surfaces with a survey of function, analytical performance, and limitations. Key words: biofi lm, sampling methods, spectrometry, immunosensors, nucleic acid-based. 21.1 Introduction Defi nitions of biofi lms vary but most investigators agree that biofi lms usually consist of four main components: (i) water, (ii) the living microbial population, (iii) the surface to which the cells are adhered, and (iv) the associated extracellular matrix, which can consist of secreted polymers (2, 17, 20, 107). These polymers, usually polysaccharides and/or protein, allow the cells to form three-dimensional structures but are not required for biofi lms to proliferate on a surface (79). Biofi -
의약/화장품용 배양배지 > Culture Media | 지더블유바이텍
PRODUCT SEARCH IR Home > > > Microbial Testing Solutions > Culture Media PDF P rint / Q&A Merck BM PM2 / [email protected] / 02-2140-3360 / - . - '' . - ‘‘ , ‘’ . Cat.No. (VAT) () BISMUTH SULFITE AGAR ACC. TO WILSON-BLAIR FOR THE ISOLATION 1.05418.0500 . AND DIFFERENTIATION OF SALMONELLA ()SELENITE CYSTINE ENRICHMENT 1.07709.0500 BROTH FOR THE ENRICHMENT OF . SALMONELLAE BAIRD-PARKER agar (base) acc. ISO 1.05406.0500 . 6888 and FDA-BAM BHI (Brain Heart Infusion) broth acc. 1.10493.0500 . ISO 6888 BPLS AGAR MODIFIED BRILLIANT- GREEN PHENOL-RED LACTOSE 1.10747.0500 . SUCROSE AGAR MODIFIED, FOR MICROBIOLOGY CETRIMIDE AGAR PSEUDOMONAS SELECTIVE AGAR BASE FOR 1.05284.0500 . MICROBIOLOGY (ACCORDING HARM. EP/USP/JP) EE BROTH-MOSSEL (ACC. HARM. EP/USP/JP) ENTEROBACTERIACEAE 1.05403.0500 . ENRICHMENT BROTH-MOSSEL FOR MICROBIOLOGY 1.08191.0500 Fluid Thioglycollate medium acc. EP, . USP, JP and ISO LETHEEN-AGAR BASE MODIFIED 1.10404.0500 . FOR MICROBIOLOGY LEVINE-EMB-AGAR FOR THE 1.01342.0500 ISOLATION AND DIFFERENTIATION . OF E.COLI AND ENTEROBACTER MALT EXTRACT AGAR FOR 1.05398.0500 . MICROBIOLOGY MANNITOL SALT PHENOL-RED AGAR 1.05404.0500 FOR MICROBIOLOGY (ACCORDING . HARM. EP/USP/JP) MEMBRANE-FILTER RINSE FLUID 1.05286.0500 . (USP) FOR MICROBIOLOGY OF BASAL MEDIUM FOR THE OF BASAL MEDIUM FOR THE DIFFERENTIATION AND 1.10282.0500 . CLASSIFICATION OF GRAM- NEGATIVE INTESTINAL BACTERIA POTATO DEXTROSE AGAR FOR 1.10130.0500 . MICROBIOLOGY PSEUDOMONAS AGAR F (BASE) FOR 1.10989.0500 . MICROBIOLOGY Rappaport-Vassiliadis Salmonella 1.07666.0500 Enrichment Broth (acc. harm. . EP/USP/JP) for microbiology RINGER TABLETS FOR THE 1.15525.0001 PREPARATION OF RINGER"S . -
Microbiology |M
Microbiology | M Description Filling Standard Pk Cat. No. Columbia sheep blood + ANC 20 ml Harm Ph 20 100253NF Columbia sheep blood agar 20 ml Harm Ph 20 100253ZF DNAse test agar 20 ml - 20 100291ZA EMB agar 20 ml ISO 21150:2006 20 100331ZA Thayer Martin agar 20 ml - 20 100353OF Hektoen enteric agar 20 ml - 20 100371ZA Legionella GVPC 20 ml ISO 11731:1998 20 100460GF Legionella BCYE non Cysteine 20 ml ISO 11731:1998 20 100460XF Legionella BCYE 20 ml - 20 100460ZF Malt extract metal agar 20 ml - 20 100544XA Malt extract agar 20 ml - 20 100544ZA Mannitol salt agar 20 ml Harm Ph 20 100560ZA MRS cysteine agar 20 ml - 20 100582JA MRS +sorbic acid agar 20 ml - 20 100582XA MRS agar 20 ml - 20 100582ZA Müller Hinton 2% NaCl agar 20 ml - 20 100611ZA Mueller Hinton blood agar 20 ml - 20 100613IF MacConkey agar 20 ml Harm Ph 20 100641ZA CT-SMAC (E.coli 0:157) agar 20 ml - 20 100683AA Nutrient agar 20 ml - 20 100692PA Standard I nutrient agar 20 ml - 20 100692ZA OGYE agar 20 ml ISO 13681:1995 20 100720ZA Orangeserum agar 20 ml - 20 100731ZA Palcam agar 20 ml EN ISO 11290-1,11290-2 20 100742ZA Plate count agar 20 ml UNE-EN ISO 4833:2003 20 100774ZA Potato dextrose agar 20 ml Harm Ph 20 100782ZA Cetrimide agar 20 ml Harm Ph 20 100793ZA Sabouraud 4% dextrose chloramphenicol agar 20 ml Harm Ph 20 100884CF Sabouraud 2% chloramphenicol actidione agar 20 ml Harm Ph 20 100884DA Sabouraud 4% glucose agar 20 ml EP, USP 20 100884ZA Salmonella Shigella agar 20 ml - 20 100911ZA Slanetz & Bartley enterococci agar 20 ml ISO 7899-2:2000 20 100980ZA TBX agar 20 ml - 20 101004ZA TCBS cholera agar 20 ml - 20 101011ZA Lactose TTC agar Tergitol-7 20 ml ISO 9308-1 20 101023ZA TSA with Cycloheximide 20 ml - 20 101114AF TSA tryptic soy agar +5% sheep blood 20 ml Harm Ph 20 101114IF Lecithin polysorbate triton x agar 20 ml - 20 101114SA TSA 20 ml Harm Ph 20 101114ZA TSC agar 20 ml UNE EN 13401 and UNE EN 26461-2 20 101141AA VRB agar 20 ml EP 20 101183ZA VRBD agar 20 ml Harm Ph 20 101203ZA XLD novobiocin agar 20 ml Harm Ph 20 101241AF XLD agar 20 ml Harm EP, USP, JP 20 101241ZF Chromocult E. -
Exploring Bacteria Diversity in Commercialized Table Olive Biofilms
www.nature.com/scientificreports OPEN Exploring bacteria diversity in commercialized table olive bioflms by metataxonomic and compositional data analysis Antonio Benítez‑Cabello1, Verónica Romero‑Gil2, Eduardo Medina‑Pradas1, Antonio Garrido‑Fernández1 & Francisco Noé Arroyo‑López1* In this work, a total of 72 samples of non-thermally treated commercial table olives were obtained from diferent markets of the world. Then, prokaryotic diversity in olive bioflms was investigated by metataxonomic analysis. A total of 660 diferent OTUs were obtained, belonging to Archaea (2.12%) and Bacteria domains (97.88%). From these, 41 OTUs with a proportion of sequences ≥ 0.01% were studied by compositional data analysis. Only two genera were found in all samples, Lactobacillus, which was the predominant bacteria in the bioflm consortium (median 54.99%), and Pediococcus (26.09%). Celerinatantimonas, Leuconostoc, Alkalibacterium, Pseudomonas, Marinilactibacillus, Weissella, and the family Enterobacteriaceae were also present in at least 80% of samples. Regarding foodborne pathogens, only Enterobacteriaceae, Vibrio, and Staphylococcus were detected in at least 91.66%, 75.00%, and 54.10% of samples, respectively, but their median values were always below 0.15%. Compositional data analysis allowed discriminating between lye treated and natural olive samples, as well as between olives packaged in glass, PET and plastic bags. Leuconostoc, Celerinatantimonas, and Alkalibacterium were the bacteria genera with a higher discriminant power among samples. These results expand our knowledge of the bacteria diversity in olive bioflms, providing information about the sanitary and hygienic status of this ready‑to‑eat fermented vegetable. Te world’s olive grove consists of more than 10 million hectares, of which over 1 million are destined to table olives, which constitute the most important fermented vegetable in the Mediterranean countries, with also noticeable productions in South America, USA and Australia. -
The Effect of the Fermented Tea Beverage Kombucha on the Gut Microflora
The effect of the fermented tea beverage kombucha on the gut microflora A double-blind placebo-controlled study Hanna Bergström Supervisor: Åsa Håkansson Assistant supervisor: Nicholas Rosenstock Examiner: Federico Gomez 2 Popular science summary The fermented tea drink kombucha has become an increasingly trendy drink during the past couple of years. Although it has historically been known for its health benefits in Asia, it has taken until recently for it to gain popularity in Western countries. Riding on the current health wave in society, kombucha is surrounded by numerous health claims, such as a high content of nutrients and beneficial fermentation products as well as being probiotic if consumed raw. Still little research has been done on the subject, meaning that few of these claims may be scientifically proven. Tightening regulations have made it harder for food producers to place health claims on their product, including claims of probiotic effects. In order for a product to be classified as probiotic, it must contain a large amount of living bacteria that survive the digestive system and reach the colon. Even though some positive health effects can come from consumption of dead bacteria, living bacteria can in some cases colonize the colon or temporarily change the gut flora to the better. Probiotics have positive effects on the gut as well as the immune system, and has recently become a hot research topic as more and more interesting connections are found. Proving a probiotic effect of a food product is thus of high relevance for the producer. This study investigated if the consumption of kombucha has an effect on the gut microbiota, and further if it depends on the content of living bacteria or is inherent even to a sterilized version of the drink. -
The Air Is Clear. Granulated Culture Media Clear the Air with Granulated Culture Media
The air is clear. Granulated Culture Media Clear the air with Granulated Culture Media The handling of powdered culture media usually results in the air borne spread of powder in the working environment. The powder of routinely used culture media often contains hazardous/toxic substances (Table1: Hazardous/toxic substances). Inhalation of powder containing hazardous/ toxic substances is unhealthy. Whilst contamination of exposed skin, eyes and ears often leads to allergic reactions. Table1: Some hazardous/toxic substances found in routinely used culture media bases Test parameters Examples of Hazardous/ culture media bases toxic substances Enterobacteriaceae Bile salt type media* Bile salt(s) E.coli Brilliant Green 2% Bile Broth Brilliant green, Bile salt Coliform bacteria Endo Agar, Fuchsin DEV Endo Agar Salmonella RVS, Diasalm, MSRV, Malachite green, Selenite Cystine Broth, Selenite Selenite Enrichment Broth acc. Leifson Tetrathionate Broths Tetrathionate, Bile salts XLT-4 Agar Tergitol Listeria Fraser Broth, Palcam Agar, Lithium chloride L-PALCAM Broth, Oxford Agar LEB Acriflavine, Cycloheximide UVM Acriflavine Staph. aureus Baird Parker Agar, Lithium chloride Giolitti Cantoni Broth, Liquid Baird Parker Enterococcus ReadyCULT® Enterococci 100 Sodium azide Kranep Agar Cycloheximide, Sodium azide, Thiocyanate Kanamycin Aesculin Azide Agar Kanamycin, Sodium azide Azide Dextrose Broth, Sodium azide Streptococci Streptococci Selective Agar, Sodium azide Streptococci Broth Yeast and moulds Selective agar for pathogenic fungi Chloramphenicol, Cycloheximide RBC, DRBC Rose bengal, Dichloran Dichloran Glycerol (DG 18) Agar Dichloran, Chloramphenicol * e.g. VRB Agar, VRBD Agar, MacConkey Agar, Enterobacteriaceae Enrichment Broth, MacConkey Purple Broth, Deoxycholate Agar, Hektoen Agar The use of g r a nulated media significantly reduces the spread of powder (Figure1: Weighing). -
Electrochemical Impedance Spectroscopy
Electrochemical impedance spectroscopy: An effective tool for a fast microbiological diagnosis # Nardo Ramírez1, Angel Regueiro2, Olimpia Arias3, Rolando Contreras1 1Dirección de Diagnóstico Microbiológico, Centro Nacional de Investigaciones Científicas, CNIC Ave. 25 esq. 158, Cubanacán, Playa, Ciudad de La Habana, Cuba FOCUS 2Centro de Bioingeniería, CEBIO 3Instituto de Ciencia y Tecnología de Materiales,Universidad de La Habana, UH E-mail: [email protected] ABSTRACT Dielectric spectroscopy, also called electrochemical impedance spectroscopy, is traditionally used in monitoring corrosion and electro-deposition processes in the coating and characterization assessment of many kinds of sensors and semi-conductors. Its application in biotechnology for the characterization of cell cultures has, however, been notably expanded in the last decade. As a transductional principle, impedance has been applied in the field of microbiology as a means of detecting and quantifying pathogenic bacteria. This paper reviews the state-of-the-art of Impedance Microbiology, its progress and its applications for the detection of foodborne pathogenic bacteria, including the use of interdigitated microelectrodes, the development of chip-based impedance microbiology and the integration of impedance biosensors along with other techniques such as dielectrophoresis and electropermeabilization. Reference is made to basic components, definitions and principles of this technique, as well as to the explanation of the components and principles for cell culture -
Efficient Separation and Sensitive Detection of Listeria
University of Arkansas, Fayetteville ScholarWorks@UARK Theses and Dissertations 12-2010 Efficient Separation and Sensitive Detection of Listeria Monocytogenes Using Magnetic Nanoparticles, Microfluidics and Interdigitated Microelectrode Based Impedance Immunosensor Damira Ashimkhanovna Kanayeva University of Arkansas Follow this and additional works at: http://scholarworks.uark.edu/etd Part of the Microbiology Commons Recommended Citation Kanayeva, Damira Ashimkhanovna, "Efficient Separation and Sensitive Detection of Listeria Monocytogenes Using Magnetic Nanoparticles, Microfluidics and Interdigitated Microelectrode Based Impedance Immunosensor" (2010). Theses and Dissertations. 194. http://scholarworks.uark.edu/etd/194 This Dissertation is brought to you for free and open access by ScholarWorks@UARK. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of ScholarWorks@UARK. For more information, please contact [email protected], [email protected]. EFFICIENT SEPARATION AND SENSITIVE DETECTION OF LISTERIA MONOCYTOGENES USING MAGNETIC NANOPARTICLES, MICROFLUIDICS AND INTERDIGITATED MICROELECTRODE BASED IMPEDANCE IMMUNOSENSOR EFFICIENT SEPARATION AND SENSITIVE DETECTION OF LISTERIA MONOCYTOGENES USING MAGNETIC NANOPARTICLES, MICROFLUIDICS AND INTERDIGITATED MICROELECTRODE BASED IMPEDANCE IMMUNOSENSOR A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Cell and Molecular Biology By Damira Ashimkhanovna Kanayeva Al-Farabi Kazakh National University -
Unraveling Microbial Biofilms of Importance for Food Microbiology
Microb Ecol (2014) 68:35–46 DOI 10.1007/s00248-013-0347-4 MINIREVIEWS Unraveling Microbial Biofilms of Importance for Food Microbiology Lizziane Kretli Winkelströter & Fernanda Barbosa dos Reis Teixeira & Eliane Pereira Silva & Virgínia Farias Alves & Elaine Cristina Pereira De Martinis Received: 15 August 2013 /Accepted: 6 December 2013 /Published online: 27 December 2013 # Springer Science+Business Media New York 2013 Abstract The presence of biofilms is a relevant risk factors in Introduction the food industry due to the potential contamination of food products with pathogenic and spoilage microorganisms. The It is known that bacteria are remarkably capable of adjusting majority of bacteria are able to adhere and to form biofilms, their needs for survival in environments. Among the microbial where they can persist and survive for days to weeks or even characteristics enabling these adaptations one of the most longer, depending on the microorganism and the environmen- important is the ability of the microorganism to form biofilms, tal conditions. The biological cycle of biofilms includes sev- since it facilitates adaptation to harsh environmental eral developmental phases such as: initial attachment, matu- conditions. ration, maintenance, and dispersal. Bacteria in biofilms are In the last decades a substantial effort has been put forth to generally well protected against environmental stress, conse- improve our understanding on microbial biofilms, defined as quently, extremely difficult to eradicate and detect in food complex and well-organized biological communities embed- industry. In the present manuscript, some techniques and ded in a self-produced extracellular polymeric matrix that can compounds used to control and to prevent the biofilm forma- develop into moist surfaces, either biotic or abiotic [102, 180]. -
Microbiology Dehydrated Culture Media & Supplements New
MICROBIOLOGY DEHYDRATED CULTURE MEDIA & SUPPLEMENTS NEW With a wide variety of first-class products, CHEMSOLUTE® offers you the perfect choice for your special needs in microbiology. TABLE OF CONTENTS GENERAL INSTRUCTIONS 4 DEHYDRATED CULTURE MEDIA 5–24 SUPPLEMENTS 25–30 MEDIA COMPONENTS 31–32 APPLICATION AREAS 33 EVERYTHING FOR MICROBIOLOGY 34 CHEMSOLUTE® Microbiology 3 GENERAL INSTRUCTIONS GENERAL INSTRUCTIONS STORAGE OF DEHYDRATED MEDIA Be aware that dehydrated media are highly hygroscopic, light-sensitive and heat-sensitive. They must be stored at a temperature of 4–30 °C, larger temperature Please check the best-before date fluctuations and direct sunlight are to be avoided. Close an open package thoroughly to on the product package before prevent moisture from getting in. storage. The date refers to the shelf life of unopened packages PREPARATION OF THE MEDIA when stored as indicated on the label. Follow the instructions on the respective label or the technical data sheet. The safety data sheet contains information on possible hazards. Place a quantity of medium powder, measured according to the manufacturing instructions (weight tolerance max. 1 %), in a clean, sterile and undamaged vessel with at least twice the final volume to allow thorough mixing. Add a portion of the required amount of distilled water and stir to dissolve the medium. Then add the remaining water from the sides of the container to wash off possible powder remains. Agar-containing media must be brought to the boil carefully and stirred to dissolve the agar before sterilization. The medium should preferably be sterilized on the day of manufacture. Unless otherwise stated, the pH of the medium does not need to be adjusted and will be within the specified pH range after sterilization. -
Microbiological Examinations Culture Media for Use in the Food Industry, Pharma and Cosmetics Or Veterinary Medicine
Microbiological examinations Culture Media for use in the food industry, pharma and cosmetics or veterinary medicine Food l Pharmaceuticals l Cosmetics l Veterinary Culture media at first-hand FOR USE IN THE FOOD INDUSTRY, PHARMA AND COSMETICS OR VETERINARY MEDICINE Culture media at first-hand We are manufacturer of more than 200 different culture media Our portfolio comprises: and supplements to cultivate the microorganisms or specifically promote the growth of particularly fastidious species. • Dehydrated culture media • Supplements and additives Thus, you are optimally equipped for the analysis of veterinary or • Ready-to-use culture media human samples. Special media for food, water, pharmaceutical • Culture media base materials products complete the portfolio. • Detection reagents Do you have a specific request? Or are you looking for a supplier of media based on your own formulation? We develop and produce according to your demand. sifin diagnostics gmbh Berliner Allee 317-321 13088 Berlin, Germany Phone: +49 30 927 030-0 Telefax: +49 30 927 030-30 E-Mail: [email protected] www.sifin.de Contents Miscellaneous Contact ................................................................................. 4 Order blank .......................................................................... 6 Certificate DIN EN ISO/IEC 17025 ........................................ 45 Culture media Culture media for food ..................................................... 7 Examination of milk, milk products and ice-cream .......... 8 Examination of natural