DOCSLIB.ORG

An Engineered Extraplastidial Pathway for Carotenoid Biofortification Of

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
An Engineered Extraplastidial Pathway for Carotenoid Biofortification Of An engineered extraplastidial pathway for carotenoid biofortification of leaves Trine Andersen, Briardo Llorente, Luca Morelli, Salvador Torres-montilla, Guillermo Bordanaba-florit, Fausto Espinosa, Maria Rosa Rodriguez-goberna, Narciso Campos, Begoña Olmedilla-alonso, Manuel Llansola-Portoles, et al. To cite this version: Trine Andersen, Briardo Llorente, Luca Morelli, Salvador Torres-montilla, Guillermo Bordanaba- florit, et al.. An engineered extraplastidial pathway for carotenoid biofortification of leaves. Plant Biotechnology Journal, Wiley, 2021, 19 (5), pp.1008-1021. 10.1111/pbi.13526. hal-03286028 HAL Id: hal-03286028 https://hal.archives-ouvertes.fr/hal-03286028 Submitted on 16 Jul 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Plant Biotechnology Journal (2021) 19, pp. 1008–1021 doi: 10.1111/pbi.13526 An engineered extraplastidial pathway for carotenoid biofortification of leaves Trine B. Andersen1,† , Briardo Llorente1,2,3 , Luca Morelli1 , Salvador Torres-Montilla1 , Guillermo Bordanaba-Florit1, Fausto A. Espinosa1, Maria Rosa Rodriguez-Goberna1, Narciso Campos1,4 , Begona~ Olmedilla-Alonso5 , Manuel J. Llansola-Portoles6 , Andrew A. Pascal6 and Manuel Rodriguez-Concepcion1,7,* 1Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain 2Department of Molecular Sciences, ARC Center of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia 3CSIRO Synthetic Biology Future Science Platform, Sydney, NSW, Australia 4Departament de Bioquı´mica i Biologia Molecular,Universitat de Barcelona, Barcelona, Spain, 08028 5Instituto de Ciencia y Tecnologıa de Alimentos y Nutricion (ICTAN-CSIC), Madrid, Spain 6CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Universite Paris-Saclay, Gif-sur-Yvette, France 7Instituto de Biologia Molecular y Celular de Plantas (IBMCP), CSIC-Universitat Politecnica de Valencia, Valencia, Spain Received 2 April 2020; Summary accepted 9 December 2020. Carotenoids are lipophilic plastidial isoprenoids highly valued as nutrients and natural pigments. *Correspondence (Tel (+34) 963 877 725; A correct balance of chlorophylls and carotenoids is required for photosynthesis and therefore + fax ( 34) 963 877 859; email highly regulated, making carotenoid enrichment of green tissues challenging. Here we show that [email protected]) leaf carotenoid levels can be boosted through engineering their biosynthesis outside the †Present address: Great Lakes Bioenergy chloroplast. Transient expression experiments in Nicotiana benthamiana leaves indicated that Research Center, Michigan State University, East Lansing, MI, 48824, USA high extraplastidial production of carotenoids requires an enhanced supply of their isoprenoid precursors in the cytosol, which was achieved using a deregulated form of the main rate- determining enzyme of the mevalonic acid (MVA) pathway. Constructs encoding bacterial enzymes were used to convert these MVA-derived precursors into carotenoid biosynthetic intermediates that do not normally accumulate in leaves, such as phytoene and lycopene. Cytosolic versions of these enzymes produced extraplastidial carotenoids at levels similar to those of total endogenous (i.e. chloroplast) carotenoids. Strategies to enhance the development of endomembrane structures and lipid bodies as potential extraplastidial carotenoid storage systems were not successful to further increase carotenoid contents. Phytoene was found to be more bioaccessible when accumulated outside plastids, whereas lycopene formed cytosolic Keywords: carotenoids, phytoene, crystalloids very similar to those found in the chromoplasts of ripe tomatoes. This extraplastidial lycopene, biosynthesis, Nicotiana production of phytoene and lycopene led to an increased antioxidant capacity of leaves. Finally, benthamiana, lettuce, bioaccessibility, we demonstrate that our system can be adapted for the biofortification of leafy vegetables such antioxidant, biofortification. as lettuce. Introduction biosynthetic pathway) are colourless (Rodriguez-Concepcion et al., 2018). Carotenoids are lipophilic isoprenoids distributed throughout all In plants, carotenoids are synthesized in plastids from the kingdoms of life. Plants and some bacteria, archaea and fungi universal C5 isoprenoid precursors isopentenyl diphosphate (IPP) can biosynthesize these compounds de novo, whereas the vast and dimethylallyl diphosphate (DMAPP) produced by the majority of animals acquire them through their diet (Rodriguez- methylerythritol 4-phosphate (MEP) pathway (Figure 1). Other Concepcion et al., 2018; Zheng et al., 2020). Carotenoids are plastidial isoprenoids, such as monoterpenoids and the prenyl an essential part of the human diet, acting as precursors of moieties of chlorophylls and tocopherols, are produced using the retinoids (including vitamin A) and health-promoting molecules. same pool of substrates. In the cytosol, a second pool of IPP and They are also economically relevant as natural pigments that DMAPP is synthesized by the mevalonic acid (MVA) pathway for provide colours in the yellow to red range to many fruits, the production of sesquiterpenoids and triterpenoids (including vegetables, seafood, fish, poultry and dairy products. One sterols). Plastid-localized isoforms of geranylgeranyl diphosphate example is lycopene, which gives rise to the red colour in ripe (GGPP) synthases catalyse the condensation of three IPP and one tomatoes. Carotenoids with a shorter conjugation length such DMAPP into one C20 GGPP. Two GGPP molecules are then as phytoene (the first committed intermediate of the converted into one C40 phytoene by phytoene synthase. 1008 ª 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Extraplastidial carotenoid synthesis 1009 Desaturation and isomerization of phytoene produce lycopene, a (crtI) resulted in the production of lycopene in the cytosol of linear carotenoid whose ends may then be cyclized into b and/or e tobacco (Nicotiana tabacum) cells (Majer et al., 2017). Virus- rings. The formation of two b rings results in the production of b- infected tissues accumulated lycopene to levels up to 10% of the carotene, from which b-b xanthophylls such as violaxanthin and total leaf carotenoid content, but only for 1–2 days, as neoxanthin are formed through oxidation. The formation of one carotenoid-producing leaves soon became necrotized. Such b and one e ring leads to the production of lutein, the most deleterious phenotype may be caused by side-effects of the viral abundant xanthophyll in photosynthetic tissues (Figure 1a). In infection, by the diversion of metabolic substrates away from chloroplasts, carotenoids are involved in light harvesting and cytosolic isoprenoid pathways, and/or by negative interference of photoprotection against excess light, and their levels are finely the lipophilic lycopene with cell membrane function. Here we balanced with those of chlorophylls for the efficient assembly and tested new strategies to circumvent these problems using functionality of photosynthetic complexes (Domonkos et al., phytoene and lycopene as the target products. The choice of 2013; Esteban et al., 2015; Hashimoto et al., 2016). Carotenoids these carotenoid intermediates (Figure 1a) was based on three can also be produced and stored at high levels in chromoplasts, main reasons. First, they are virtually absent in non-engineered which are specialized plastids found in some non-photosynthetic leaves as they are readily converted into downstream chloroplast tissues of flowers, fruits and other carotenoid-accumulating carotenoids (Domonkos et al., 2013; Esteban et al., 2015; tissues (Sadali et al., 2019; Sun et al., 2018). Hashimoto et al., 2016; Rodriguez-Concepcion et al., 2018). Several biotechnological strategies have been tested to enrich Second, their supply is essential for the eventual production of plant-derived feed and food products with carotenoids to any downstream carotenoid of interest. And third, they are improve their nutritional and economic value (Alos et al., 2016; health-promoting phytonutrients that are only found in a few Zheng et al., 2020). While highly successful results have been food sources (Melendez-Martinez et al., 2015; Melendez- obtained in non-photosynthetic tissues, including Golden Rice, Martinez et al., 2018; Muller€ et al., 2011; Rodriguez-Concepcion manipulation of carotenoid levels in photosynthetic tissues has et al., 2018). been much more challenging. We have investigated methods to produce carotenoids in green tissues without interfering with Results photosynthetic function by moving their biosynthesis away from Agroinfiltrated leaves can produce extraplastidial
Load more

Recommended publications
  • Association Between Serum Concentration of Carotenoid and Visceral Fat
    nutrients Article Association between Serum Concentration of Carotenoid and Visceral Fat Mai Matsumoto 1,*, Hiroyuki Suganuma 1 , Naoki Ozato 2,3 , Sunao Shimizu 1,4,5 , Mitsuhiro Katashima 2,3, Yoshihisa Katsuragi 2,3, Tatsuya Mikami 5, Ken Itoh 4,6 and Shigeyuki Nakaji 5,7 1 Innovation Division, KAGOME CO. LTD., 17 Nishitomiyama, Nasushiobara, Tochigi 329-2762, Japan; [email protected] (H.S.); [email protected] (S.S.) 2 Department of Active Life Promotion Sciences, Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; [email protected] (N.O.); [email protected] (M.K.); [email protected] (Y.K.) 3 Health & Wellness Products Research Laboratories, Kao Corporation, Tokyo 131-8501, Japan 4 Department of Vegetable Life Science, Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; [email protected] 5 Innovation Center for Health Promotion, Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; [email protected] (T.M.); [email protected] (S.N.) 6 Department of Stress Response Science, Center for Advanced Medical Research, Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan 7 Department of Social Medicine, Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan * Correspondence: [email protected]; Tel.: +81-80-1581-1874 Abstract: Consumption of fruits and vegetables rich in carotenoids has been widely reported to prevent cardiovascular diseases. However, the relationship between serum carotenoid concentrations Citation: Matsumoto, M.; Suganuma, and visceral fat area (VFA), which is considered a better predictor of cardiovascular diseases than H.; Ozato, N.; Shimizu, S.; Katashima, the body-mass index (BMI) and waist circumference, remains unclear.
    [Show full text]
  • Genetics and Molecular Biology of Carotenoid Pigment Biosynthesis
    SERIAL REVIEW CAROTENOIDS 2 Genetics and molecular biology of carotenoid pigment biosynthesis GREGORY A. ARMSTRONG,’1 AND JOHN E. HEARSTt 5frtitute for Plant Sciences, Plant Genetics, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland; and tDePai.tment of Chemistry, University of California, and Structural Biology Division, Lawrence Berkeley Laboratory, Berkeley, California 94720, USA The two major functions of carotenoids in photosyn- carotenoid biosynthesis from a molecular genetic thetic microorganisms and plants are the absorption of standpoint.-Armstrong, G. A., Hearst, J. E. Genet- energy for use in photosynthesis and the protection of ics and molecular biology of carotenoid pigment chlorophyll from photodamage. The synthesis of vari- biosynthesis. F14SEBJ. 10, 228-237 (1996) ous carotenoids, therefore, is a crucial metabolic proc- ess underlying these functions. In this second review, Key Words: phytoene lycopene cyclization cyclic xanthophylLs the nature of these biosynthetic pathways is discussed xanlhophyll glycosides’ 3-carotene provitamin A in detail. In their elucidation, molecular biological techniques as well as conventional enzymology have CAROTENOIDS REPRESENT ONE OF THE most fascinating, played key roles. The reasons for some of the ci.s-t Tans abundant, and widely distributed classes of natural pig- isomerizations in the pathway are obscure, however, ments. Photosynthetic organisms from anoxygenic photo- and much still needs to be learned about the regula- synthetic bacteria through cyanobacteria, algae, and tion of carotenoid biosynthesis. Recent important find- higher plants, as well as numerous nonphotosynthetic ings, as summarized in this review, have laid the bacteria and fungi, produce carotenoids (1). Among groundwork for such studies. higher plants, these pigments advertise themselves in flowers, fruits, and storage roots exemplified by the yel- -James Olson, Coordinating Editor low, orange, and red pigments of daffodils, carrots and to- matoes, respectively.
    [Show full text]
  • Meet Lycopene Prostate Cancer Is One of the Leading Causes of Cancer Death Among Men in the United States
    UCLA Nutrition Noteworthy Title Lycopene and Mr. Prostate: Best Friends Forever Permalink https://escholarship.org/uc/item/5ks510rw Journal Nutrition Noteworthy, 5(1) Author Simzar, Soheil Publication Date 2002 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Meet Lycopene Prostate cancer is one of the leading causes of cancer death among men in the United States. Dietary factors are considered an important risk factor for the development of prostate cancer in addition to age, genetic predisposition, environmental factors, and other lifestyle factors such as smoking. Recent studies have indicated that there is a direct correlation between the occurrence of prostate cancer and the consumption of tomatoes and tomato-based products. Lycopene, one of over 600 carotenoids, is one of the main carotenoids found in human plasma and it is responsible for the red pigment found in tomatoes and other foods such as watermelons and red grapefruits. It has been shown to be a very potent antioxidant, with oxygen-quenching ability greater than any other carotenoid. Recent research has indicated that its antioxidant effects help lower the risk of heart disease, atherosclerosis, and different types of cancer-especially prostate cancer. Lycopene's Characteristics Lycopene is on of approximately 600 known carotenoids. Carotenoids are red, yellow, and orange pigments which are widely distributed in nature and are especially abundant in yellow- orange fruits and vegetables and dark green, leafy vegetables. They absorb light in the 400- 500nm region which gives them a red/yellow color. Only green plants and certain microorganisms such as fungi and algae can synthesize these pigments.
    [Show full text]
  • Dietary Factors That Affect Carotenoid Bioavailability' Van Karin H
    Dietaryfactor stha taffec t carotenoid bioavailability KarinH .va n hetHo f Promotoren: Dr. J.G.A.J. Hautvast Hoogleraar ind e leer van devoedin g en gezondheid, Landbouwuniversiteit Wageningen Dr. C.E. West Universitair hoofddocent aan de afdeling Humane Voeding en Epidemiologie, Departement Levensmiddelentechnologiee n Voedingswetenschappen, Landbouwuniversiteit Wageningen Visiting Professor of International Nutrition, Rollins School of Public Health, Emory University, Atlanta GA, USA Co-promotor: Dr. Ir.J.A . Weststrate Unilever Research Vlaardingen /J r'. Stellingen behorend bij het proefschrift 'Dietary factors that affect carotenoid bioavailability' van Karin H. van het Hof. Wageningen,4 jun i 1999. 1. "Natuurlijk" wordt vaak ten onrechte verward met gezond; wat betreft de biobeschikbaarheid van carotenoTden zijn juist de "onnatuurlijke" en technologisch bewerkte bronnen beter (ditproefschrift). 2. Optimalisatie van de biobeschikbaarheid van carotenoTden is geen geldig excuus voor eenvetrijk e maaltijd (ditproefschrift). 3. Homogenisatie verbetert de biobeschikbaarheid van carotenoTden uit groenten. De Hollandse stamppot isdaaro mz o slecht nog niet (ditproefschrift). 4. Een beperking in de aanwezigheid van gevalideerde biomarkers en gevoelige analysemethoden is een limiterende factor voor de vooruitgang in de voedingswetenschap. 5. Het gebruik van functional foods noopt tot een verdergaande individualisering van het eetgedrag. 6. Zolang een meerderheid van de vrouwen valt op mannen die ouder en minstens even intelligent zijn alszijzelf , vindt emancipatie aan deto p van organisaties geendoorgang . 7. Voor sommige mensen veroorzaakt de stiptheid van de Nederlandse Spoorwegen meerstres s danzij nvertragingen . OM'V ?0' , Dietaryfactor stha t affect carotenoid bioavailability Katharina Henrietteva n hetHo f Proefschrift ter verkrijging van degraa dva n doctor op gezag van de rector magnificus, vand e Landbouwuniversiteit Wageningen, dr.
    [Show full text]
  • Enhanced Lycopene Production in Escherichia Coli by Expression of Two MEP Pathway Enzymes from Vibrio Sp
    catalysts Article Enhanced Lycopene Production in Escherichia coli by Expression of Two MEP Pathway Enzymes from Vibrio sp. Dhg 1, 1, 1 1, Min Jae Kim y, Myung Hyun Noh y , Sunghwa Woo , Hyun Gyu Lim * and Gyoo Yeol Jung 1,2,* 1 Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea; [email protected] (M.J.K.); [email protected] (M.H.N.); [email protected] (S.W.) 2 School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea * Correspondence: [email protected] (H.G.L.); [email protected] (G.Y.J.); Tel.: +82-54-279-2391 (G.Y.J.) These authors contributed equally to this work. y Received: 28 October 2019; Accepted: 26 November 2019; Published: 29 November 2019 Abstract: Microbial production is a promising method that can overcome major limitations in conventional methods of lycopene production, such as low yields and variations in product quality. Significant efforts have been made to improve lycopene production by engineering either the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway or mevalonate (MVA) pathway in microorganisms. To further improve lycopene production, it is critical to utilize metabolic enzymes with high specific activities. Two enzymes, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and farnesyl diphosphate synthase (IspA), are required in lycopene production using MEP pathway. Here, we evaluated the activities of Dxs and IspA of Vibrio sp. dhg, a newly isolated and fast-growing microorganism.
    [Show full text]
  • Vitamins a and E and Carotenoids
    Fat-Soluble Vitamins & Micronutrients: Vitamins A and E and Carotenoids Vitamins A (retinol) and E (tocopherol) and the carotenoids are fat-soluble micronutrients that are found in many foods, including some vegetables, fruits, meats, and animal products. Fish-liver oils, liver, egg yolks, butter, and cream are known for their higher content of vitamin A. Nuts and seeds are particularly rich sources of vitamin E (Thomas 2006). At least 700 carotenoids—fat-soluble red and yellow pigments—are found in nature (Britton 2004). Americans consume 40–50 of these carotenoids, primarily in fruits and vegetables (Khachik 1992), and smaller amounts in poultry products, including egg yolks, and in seafoods (Boylston 2007). Six major carotenoids are found in human serum: alpha-carotene, beta-carotene, beta-cryptoxanthin, lutein, trans-lycopene, and zeaxanthin. Major carotene sources are orange-colored fruits and vegetables such as carrots, pumpkins, and mangos. Lutein and zeaxanthin are also found in dark green leafy vegetables, where any orange coloring is overshadowed by chlorophyll. Trans-Lycopene is obtained primarily from tomato and tomato products. For information on the carotenoid content of U.S. foods, see the 1998 carotenoid database created by the U.S. Department of Agriculture and the Nutrition Coordinating Center at the University of Minnesota (http://www.nal.usda.gov/fnic/foodcomp/Data/car98/car98.html). Vitamin A, found in foods that come from animal sources, is called preformed vitamin A. Some carotenoids found in colorful fruits and vegetables are called provitamin A; they are metabolized in the body to vitamin A. Among the carotenoids, beta-carotene, a retinol dimer, has the most significant provitamin A activity.
    [Show full text]
  • Free Radicals in Biology and Medicine Page 0
    77:222 Spring 2005 Free Radicals in Biology and Medicine Page 0 This student paper was written as an assignment in the graduate course Free Radicals in Biology and Medicine (77:222, Spring 2005) offered by the Free Radical and Radiation Biology Program B-180 Med Labs The University of Iowa Iowa City, IA 52242-1181 Spring 2005 Term Instructors: GARRY R. BUETTNER, Ph.D. LARRY W. OBERLEY, Ph.D. with guest lectures from: Drs. Freya Q . Schafer, Douglas R. Spitz, and Frederick E. Domann The Fine Print: Because this is a paper written by a beginning student as an assignment, there are no guarantees that everything is absolutely correct and accurate. In view of the possibility of human error or changes in our knowledge due to continued research, neither the author nor The University of Iowa nor any other party who has been involved in the preparation or publication of this work warrants that the information contained herein is in every respect accurate or complete, and they are not responsible for any errors or omissions or for the results obtained from the use of such information. Readers are encouraged to confirm the information contained herein with other sources. All material contained in this paper is copyright of the author, or the owner of the source that the material was taken from. This work is not intended as a threat to the ownership of said copyrights. S. Jetawattana Lycopene, a powerful antioxidant 1 Lycopene, a powerful antioxidant by Suwimol Jetawattana Department of Radiation Oncology Free Radical and Radiation Biology The University
    [Show full text]
  • Induction of Carotenoid and Phytosterol Accumulation in Microalgae
    Induction of carotenoid and phytosterol accumulation in microalgae Faruq Ahmed Master of Marine Bioscience A thesis submitted for the degree of Doctor of Philosophy at The University of Queensland in 2015 School of Agriculture and Food Sciences 1 Abstract Microalgae have become well-known in the last few decades as producers of various compounds of high nutritional value, such as long chain polyunsaturated fatty acids, pigments including carotenoids, proteins, sterols, and vitamins. Among these compounds, carotenoids and phytosterols have been receiving increased attention based on the discoveries that they are capable of preventing various diseases such as cardiovascular problems, certain cancers, and neurological disorders, e.g. Alzheimer’s, amyotrophic lateral sclerosis etc. The commercial exploitation of the microalgal strains Dunaliella salina and Haematococcus pluvialis for β-carotene and astaxanthin, respectively, led to the hypothesis for the current study that other microalgal strains may even have a higher potential to produce these compounds, especially when the underlying physiological pathways that lead to elevated levels of these compounds could be stimulated. The aims of this thesis were therefore (1) to screen microalgal strains for their potential to produce carotenoids and phytosterols and (2) to induce the top producer(s) to accumulate higher quantities of carotenoids and phytosterols and (3) to scale up the successful induction method(s) to explore large-scale commercial production of these metabolites. Moreover, the well-known sensitive nature of carotenoids (e.g. astaxanthin and β-carotene) to light, drying and preservation methods led to the idea that freeze-drying rather than the currently popular spray-drying methods could be more suitable for long-term storage of highly valuable carotenoids, such as astaxanthin.
    [Show full text]
  • Petition to Include Synthetic Crystalline LYCOPENE at 7 CFR 205.605
    Petition to Include Synthetic Crystalline LYCOPENE at 7 CFR 205.605 Item A This petition seeks inclusion of Synthetic Crystalline LYCOPENE on the National List as a non-agricultural (non-organic) substance allowed in or on processed products labeled as “organic” or “made with organic (specified ingredients),” at §205.605(b). Item B 1. The substance‟s chemical or material common name. Lycopene is a naturally occurring aliphatic hydrocarbon of the carotenoid class. Lycopene is the most abundant carotenoid in ripe tomatoes and comprises 80-90% of the total pigment. Lycopene contains thirteen double bonds. The all-trans isomer is predominant in tomatoes and other natural sources. Storage, cooking, food processing, and exposure to light may result in some isomerization of the all-trans form to various cis forms including the 5-cis, 9-cis, 13-cis, and 15- cis forms. Synthetic crystalline lycopene is predominantly the all trans-lycopene (>70%) with some 5-cis- lycopene and other cis isomers. Other chemical names for lycopene are ψ,ψ-carotene and (all-E)-all-trans-lycopene. It is commonly known as all-trans-lycopene. The systematic name for the all-trans-lycopene is (all- E)-2, 6, 10, 14, 19, 23, 27, 31-octamethyl-2,6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 30- dotricaconta-tridecaene. 2a. The name and address of the manufacturer/producer of the substance. Synthetic crystalline lycopene is produced by: BASF SE Carl-Bosch-Straße 38 67056 Ludwigshafen/Germany 2b. The name, address and telephone number and other contact information of the petitioner. International Formula Council 1100 Johnson Ferry Road NE, Suite 300 Atlanta, GA 30342 Contact: Mardi Mountford, Executive Vice President Phone: (678) 303-3027 Email: [email protected] Petition to Include Synthetic Crystalline LYCOPENE at 7 CFR 205.605 3.
    [Show full text]
  • Simultaneous Determination of Pigments, Tocopherols, and Squalene in Greek Olive Oils: a Study of the Influence of Cultivation and Oil-Production Parameters
    foods Article Simultaneous Determination of Pigments, Tocopherols, and Squalene in Greek Olive Oils: A Study of the Influence of Cultivation and Oil-Production Parameters Ioannis Martakos, Marios Kostakis, Marilena Dasenaki * , Michalis Pentogennis and Nikolaos Thomaidis Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian, University of Athens, Panepistimiopolis Zographou, Athens 15771, Greece; [email protected] (I.M.); [email protected] (M.K.); [email protected] (M.P.); [email protected] (N.T.) * Correspondence: [email protected]; Tel.: +30-210-7274430; Fax: +30-210-727475 Received: 4 November 2019; Accepted: 17 December 2019; Published: 29 December 2019 Abstract: A new facile and fast method was developed in this study for the determination of pigments (chlorophylls and carotenoids), tocopherols (α-, sum of (β + γ), and δ), and squalene in olive oil. This method consisted of a dilution of olive oil in 2-propanol, followed by reversed phase-high-pressure liquid chromatography equipped with a diode array detector (RP-HPLC-DAD). Chromatographic separation was performed using a C18 column, while the mobile phase consisted of acetonitrile and methanol using a gradient elution program. The methodology was optimized, validated, and applied to the analysis of 452 samples of Extra Virgin Olive Oil (EVOOs) and Virgin Olive Oil (VOOs) originated from five islands of the Northeastern Aegean Region, in Greece. From the obtained results, it was indicated that the carotenoid, tocopherol, and squalene content was relatively high, while the chlorophyll content was low. Furthermore, the acquired results were studied and compared in order to obtain useful information about the correlation of the concentration levels of these compounds in olive oil to different cultivation and olive oil production parameters.
    [Show full text]
  • Overview of Carotenoid Bioavailability Determinants: from Dietary Factors to Host Genetic Variations Charles Desmarchelier, Patrick Borel
    Overview of carotenoid bioavailability determinants: From dietary factors to host genetic variations Charles Desmarchelier, Patrick Borel To cite this version: Charles Desmarchelier, Patrick Borel. Overview of carotenoid bioavailability determinants: From dietary factors to host genetic variations: Carotenoid bioavailability determinants. Trends in Food Science and Technology, Elsevier, 2017, 69 (B), Epub ahead of print. 10.1016/j.tifs.2017.03.002. inserm-01499484 HAL Id: inserm-01499484 https://www.hal.inserm.fr/inserm-01499484 Submitted on 1 Oct 2017 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives| 4.0 International License Accepted Manuscript Overview of carotenoid bioavailability determinants: From dietary factors to host genetic variations Charles Desmarchelier, Patrick Borel PII: S0924-2244(16)30523-4 DOI: 10.1016/j.tifs.2017.03.002 Reference: TIFS 1977 To appear in: Trends in Food Science & Technology Received Date: 16 November 2016 Revised Date: 11 January 2017 Accepted Date: 10 March 2017 Please cite this article as: Desmarchelier, C., Borel, P., Overview of carotenoid bioavailability determinants: From dietary factors to host genetic variations, Trends in Food Science & Technology (2017), doi: 10.1016/j.tifs.2017.03.002.
    [Show full text]
  • Spreads Enriched with Three Different Levels of Vegetable Oil Sterols And
    European Journal of Clinical Nutrition (1999) 53, 319±327 ß 1999 Stockton Press. All rights reserved 0954±3007/99 $12.00 http://www.stockton-press.co.uk/ejcn Spreads enriched with three different levels of vegetable oil sterols and the degree of cholesterol lowering in normocholesterolaemic and mildly hypercholesterolaemic subjects HFJ Hendriks1*, JA Weststrate2, T van Vliet1 and GW Meijer2 1TNO Nutrition and Food Research Institute, Zeist, The Netherlands; and 2The Unilever Nutrition Center, Unilever Research, Vlaardingen, The Netherlands Objective: To investigate the dose-response relationship between cholesterol lowering and three different, relatively low intake levels of plant sterols (0.83, 1.61, 3.24 g=d) from spreads. To investigate the effects on lipid- soluble (pro)vitamins. Design: A randomized double-blind placebo controlled balanced incomplete Latin square design using ®ve spreads and four periods. The ®ve study spreads included butter, a commercially available spread and three experimental spreads forti®ed with three different concentrations of plant sterols. Subjects: One hundred apparently healthy normocholesterolaemic and mildly hypercholesterolaemic volunteers participated. Interventions: Each subject consumed four spreads, each for a period of 3.5 week. Results: Compared to the control spread, total cholesterol decreased by 0.26 (CI: 0.15 ± 0.36), 0.31 (CI: 0.20 ± 0.41) and 0.35 (CI: 0.25 ± 0.46) mmol=L, for daily consumption of 0.83, 1.61 and 3.24 g plant sterols, respectively. For LDL-cholesterol these decreases were 0.20 (CI: 0.10 ± 0.31), 0.26 (CI: 0.15 ± 0.36) and 0.30 (CI: 0.20 ± 0.41).
    [Show full text]