The First Cytogenetic Study of the Malayan Porcupine, Hystrix Brachyuran (Rodentia, Hystricidae) by Conventional Staining and G-Banding Technique
© 2008 The Japan Mendel Society Cytologia 73(3): 221–228, 2008 The First Cytogenetic Study of the Malayan porcupine, Hystrix brachyuran (Rodentia, Hystricidae) by Conventional Staining and G-banding Technique Alongkoad Tanomtong1,*, Praween Supanuam1, Wiwat Sangpakdee1, Pornnarong Siripiyasing2, Pawarisa Boonhan1 and Sarawut Kaewsri3 1 Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand 2 Major of Biology, Faculty of Science and Technology, Mahasarakham Rajabhat University, Muang, Mahasarakham 44000, Thailand 3 Program in Applied Biology, Department of Science, Faculty of Science, Buriram Rajabhat University, Muang, Buriram 31000, Thailand Received May 24, 2008; accepted July 12, 2008 Summary This research was the first cytogenetic study of the Malayan porcupine (Hystrix brachyura) from Songkla Zoo, Thailand. Blood samples were taken from two males and two females and then subjected to standard whole blood T-lymphocyte culture. The samples were harvested by colchicine-hypotonic-fixation-air-drying technique and followed by conventional staining and G- banding with Giemsa’s. The results showed that the diploid number was 2nϭ66, and the fundamental number (NF) was 127 and 128 in male and female, respectively. The autosomes consist of 6 large submetacentric, 20 large acrocentric, 6 medium submetacentric, 18 medium acrocentric, 2 medium telocentric, 4 small submetacentric, 6 small acrocentric and 2 small telocentric chromosomes. We found the nucleolar organizer regions (NORs), the representative chromosome marker, which are lo- cated on the long arms of the pair submetacentric autosomes 9 and 13. The X chromosome was a large metacentric chromosome, while the Y chromosome was the smallest telocentric chromosome. G-banding technique indicated that the number of bands was 236.
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