![DICHLOROACETIC ACID [Acetic Acid, Dichloro-]](http://data.docslib.org/img/7d08d6cd5f5b7d444d64f085938ef14b-1.webp)
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Organic Syntheses, Coll. Vol. 2, p.181 (1943); Vol. 19, p.38 (1939). DICHLOROACETIC ACID [Acetic acid, dichloro-]
Submitted by Arthur C. Cope, John R. Clark, and Ralph Connor. Checked by R. L. Shriner and Neil S. Moon. 1. Procedure A solution of 250 g. (1.5 moles) of u.s.p. chloral hydrate in 450 cc. of warm water (50–60°) is placed in a 3-l. round-bottomed flask bearing a reflux condenser and thermometer (Note 1). The condenser is temporarily removed and 152.5 g. (1.52 moles) of precipitated calcium carbonate added; this is followed by 2 cc. of amyl alcohol (Note 2) and a solution of 10 g. of technical sodium cyanide in 25 cc. of water. Although the reaction is exothermic, the reaction mixture is heated with a low flame so that it reaches 75° in about ten minutes; at this point heating is discontinued. The temperature continues to rise to 80–85° during five to ten minutes and then drops. As soon as the temperature begins to fall the solution is heated to boiling and refluxed for twenty minutes. The mixture is then cooled to 0–5° in an ice bath, acidified with 215 cc. of concentrated hydrochloric acid (sp. gr. 1.18), and extracted with five 100-cc. portions of ether (Note 3). The combined ether extracts are dried with 20 g. of anhydrous sodium sulfate, the ether is removed by distillation from a steam bath, and the residue is distilled in vacuum from a Claisen flask with a fractionating side arm (Note 4). The yield of dichloroacetic acid, b.p. 99–104°/23 mm., is 172–180 g. (88–92 per cent of the theoretical amount) (Note 5). 2. Notes 1. The amount of hydrogen cyanide evolved is small, and the reaction may be carried out in a hood without any special device for removing this gas. The use of mechanical stirring does not improve the results. 2. Amyl alcohol is added to decrease the amount of foaming. 3. The emulsion which often forms during the ether extraction may be broken by filtering through a fluted filter or with suction. 4. The product decomposes when distilled at atmospheric pressure. 5. The preparation has been carried out with equally good results using double the quantities given above. 3. Discussion Dichloroacetic acid has been prepared by the chlorination of acetic1 or chloroacetic2 acid, by hydrolysis of pentachloroethane,3 from trichloroacetic acid by electrolytic reduction4 or the action of copper,5 and by the action of alkali cyanides on chloral hydrate.6 The method described here is essentially that of Delépine.7
References and Notes 1. Müller, Ann. 133, 159 (1865); Dow Chemical Company, U. S. pat. 1,921,717 [C. A. 27, 5084 (1933)]. 2. Maumené, Compt. rend. 59, 84 (1864). 3. Alais, Froges, and Camargue, Fr. pat. 773, 623 [C. A. 29, 1437 (1935)]. 4. Brand, Ger. pat. 246,661 [C. A. 6, 2496 (1912)]. 5. Doughty and Black, J. Am. Chem. Soc. 47, 1091 (1925); Doughty and Derge, ibid. 53, 1594 (1931). 6. Wallach, Ann. 173, 288 (1874); Pucher, J. Am. Chem. Soc. 42, 2251 (1920); Chattaway and Irving, J. Chem. Soc. 1929, 1038. 7. Delépine, Bull. soc. chim. (4) 45, 827 (1929).
Appendix Chemical Abstracts Nomenclature (Collective Index Number); (Registry Number) hydrochloric acid (7647-01-0) ether (60-29-7) sodium cyanide (143-33-9) hydrogen cyanide (74-90-8) sodium sulfate (7757-82-6) copper (7440-50-8) calcium carbonate (471-34-1) chloral hydrate (302-17-0) amyl alcohol (71-41-0) dichloroacetic acid, Acetic acid, dichloro- (79-43-6) pentachloroethane (76-01-7) trichloroacetic acid (76-03-9)
Dichloroacetic acid
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Jump to: navigation, search Dichloroacetic acid
IUPAC name [hide]
Dichloroacetic acid
Other names[hide]
Dichloroethanoic acid
Identifiers CAS number 79-43-6 PubChem 6597 ChemSpider 10771217 UNII 9LSH52S3LQ DrugBank DB08809 KEGG C11149 MeSH Dichloroacetate ChEBI CHEBI:36386 ChEMBL CHEMBL13960 RTECS number AG6125000 Jmol-3D images Image 1 SMILES [show]
InChI [show]
Properties
Molecular formula C2H2Cl2O2 Molar mass 128.94 g mol−1 Appearance Colorless liquid Density 1.5634 g/cm3 (20 °C) 9-11 °C, 282-284 K, 48-52 °F Melting point 194 °C, 467 K, 381 °F Boiling point Solubility in water miscible Solubility miscible with ethanol, diethyl ether [1]
Acidity (pKa) 1.35[1] Thermochemistry Std enthalpy of -1 o -496.3 kJ·mol [1] formation ΔfH 298 Hazards MSDS MSDS (jtbaker) R-phrases R35 R50 S-phrases (S1/2) S26 S45 S61
1 NFPA 704 3 Related compounds Chloroacetic acid Related chloroacetic acids Trichloroacetic acid Acetic acid Related compounds Difluoroacetic acid Dibromoacetic acid (verify) (what is: / ?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox references Dichloroacetic acid, often abbreviated DCA, is the chemical compound with formula C H Cl 2COOH. It is an acid, an analogue of acetic acid, in which two of the three hydrogen atoms of the methyl group have been replaced by chlorine atoms. The salts and esters of dichloroacetic acid are called dichloroacetates. Salts of DCA have been studied as potential drugs because they inhibit the enzyme pyruvate dehydrogenase kinase.[citation needed] Although preliminary studies have shown DCA can slow the growth of certain tumors in animal studies and in vitro studies, "Available evidence does not support the use of DCA for cancer treatment at this time."[2] Contents • 1 Chemis try and occurre nce • 2 Therap eutic use • 2 . 1
L a c t i c
a c i d o s i s
• 3 Potenti al cancer applicat ions • 3 . 1
R e s u l t s
o f
Chemistry and occurrence The chemistry of dichloroacetic acid is typical for halogenated organic acids. It is a member of the chloroacetic acids family. The dichloroacetate ion is produced when the acid is mixed with water. As an acid with a pKa of 1.35,[1] pure dichloroacetic acid is very corrosive and extremely destructive to tissues of the mucous membranes and upper respiratory tract via inhalation.[3] DCA does not occur in nature. It is a trace product of the chlorination of drinking water and is produced by the metabolism of various chlorine-containing drugs or chemicals.[4] DCA is typically prepared by the reduction of trichloroacetic acid. DCA is prepared from chloral hydrate also by the reaction with calcium carbonate and sodium cyanide in water followed by acidifying with hydrochloric acid.
Therapeutic use Owing to the highly corrosive action of the acid, only the salts of dichloroacetic acid are used therapeutically, including its sodium and potassium salts, sodium dichloroacetate and potassium dichloroacetate.
Lactic acidosis The dichloroacetate ion stimulates the activity of the enzyme pyruvate dehydrogenase by inhibiting the enzyme pyruvate dehydrogenase kinase.[5] Thus, it decreases lactate production by shifting the metabolism of pyruvate from fermentation towards oxidation in the mitochondria. This property has led to trials of DCA for the treatment of lactic acidosis in humans. [6] [7] [8] [9] A randomized controlled trial in children with congenital lactic acidosis found that while DCA was well tolerated, it was ineffective in improving clinical outcomes.[7] A separate trial of DCA in children with MELAS (a syndrome of inadequate mitochondrial function, leading to lactic acidosis) was halted early, as all 15 of the children receiving DCA experienced significant nerve toxicity without any evidence of benefit from the medication.[8] A randomized controlled trial of DCA in adults with lactic acidosis found that while DCA lowered blood lactate levels, it had no clinical benefit and did not improve hemodynamics or survival.[9] Thus, while early case reports and pre-clinical data suggested that DCA might be effective for lactic acidosis, subsequent controlled trials have found no clinical benefit of DCA in this setting. In addition, clinical trial subjects were incapable of continuing on DCA as a study medication owing to progressive toxicities.
Potential cancer applications Cancer cells generally express increased glycolysis, because they rely on anaerobic respiration that occurs in the cytosol (lactic acid fermentation) rather than oxidative phosphorylation in the mitochondria for energy (the Warburg effect), as a result of hypoxia that exists in tumors and malfunctioning mitochondria. [10] [11] Usually dangerously damaged cells kill themselves via apoptosis, a mechanism of self-destruction that involves mitochondria, but this mechanism fails in cancer cells. A phase I study published in January 2007 by researchers at the University of Alberta, who had tested DCA on human[12] cancer cells grown in mice, found that DCA restored mitochondrial function, thus restoring apoptosis, allowing cancer cells to self-destruct and shrink the tumor.[13] These results received extensive media attention, beginning with an article in New Scientist titled "Cheap, ‘safe’ drug kills most cancers".[12] Subsequently, the American Cancer Society and other medical organizations have received a large volume of public interest and questions regarding DCA.[14] Clinical trials in humans with cancer have not been conducted in the USA and are not yet final in Canada, emphasizing the need for caution in interpreting the preliminary results. [14] [15]
Results of phase II clinical trials In in vitro studies, Evangelos Michelakis of University of Alberta found that in tissue samples from 49 patients, DCA caused depolarization of mitochondria in GBM tissue but not in healthy brain tissue.[16] Five patients with primary GBM were entered into a phase II trial. Three had not responded to several chemotherapies and was considered appropriate for palliative care. Two were newly diagnosed and then went through surgical removal of tumour mass. All of them were treated with DCA and chemotherapy.[16] Of the five patients tested, one of the first three died after three months. The surviving four were followed for 15 months. Their Karnofsky scores were unchanged in two cases, and decreased by 10 points in two patients.[16] DCA was associated with tumor regression and had a good safety profile. DCA side effects were minimal.[16] Michelakis is proceeding with phase three human studies with private funding from philanthropic groups and individuals. DCA's legal status as a discovery is public domain because it was made or discovered as far back as 1864[17] and has been used in the treatment of canine and human lactic acidosis, some who presented at the beginning of treatment with cancer.
Concerns about pre-trial use Following its initial publication, The New Scientist later editorialized, "The drug may yet live up to its promise as an anti-cancer agent – clinical trials are expected to start soon. It may even spawn an entirely new class of anti-cancer drugs. For now, however, it remains experimental, never yet properly tested in a person with cancer. People who self-administer the drug are taking a very long shot and, unlikely as it may sound, could even make their health worse."[18] In 2010, it was found that for human colorectal tumours grown in mice, under hypoxic conditions, DCA decreased rather than increased apoptosis, resulting in enhanced growth of the tumours.[19] These findings suggest that at least in some cancer types DCA treatment could be detrimental to patient health, highlighting the need for further testing before it can be considered a safe and effective cancer treatment.[19]
Planned and ongoing clinical trials DCA is non-patentable as a compound, though a patent has been filed for its use in cancer treatment.[20] Research by Evangelos Michelakis has received no support from the pharmaceutical industry.[21] Concerns have been raised that without strong intellectual property protection, the financial incentive for drug development is reduced, and therefore obtaining sufficient funds to conduct clinical trials presents difficulty. [12] [14] [15] [22] However, other sources of funding exist; previous studies of DCA have been funded by government organizations such as the National Institutes of Health, the Food and Drug Administration, the Canadian Institutes of Health Research and by private charities (e.g. the Muscular Dystrophy Association). Recognizing anticipated funding challenges, Michelakis's lab took the unorthodox step of directly soliciting online donations to fund the research.[23] After 6 months, his lab had raised over $800,000, enough to fund a small Clinical Phase 2 study. Michelakis and Archer have applied for a patent on the use of DCA in the treatment of cancer. [20] [24] On 24 September 2007, the University of Alberta's Department of Medicine reported that after the trial funding was secured, both the Alberta local ethics committee and Health Canada approved the first DCA clinical trial for cancer.[25] This initial trial was relatively small with enrollment of up to 50 patients. The trial was completed in August 2009.[26] In May 2010 the team published a press release[27] stating no conclusions could be drawn as a result of the trial. A paper describing the results was published[28] but not linked from the press release. Only five patients had been treated with the drug during the trial. In May 2011, online reports[29] suggested that the Alberta group had released new data which the media "had not reported". However, this appeared to be caused by confusion between dates (the previous update was May 2010[30]) and cancer charities moved quickly to counter these rumours, [31] [32] which were subsequently covered in New Scientist magazine.[33] The use of this compound as an anti-cancer agent has been patented.[34]
Side effects Reports in the lay press after the 2007 University of Alberta announcement claim that dichloroacetate "has actually been used safely in humans for decades",[35] DCA is generally well tolerated, even in children.[36] Short-term, infused, bolus doses of DCA at 50 mg/kg/day have been well tolerated.[37] At sustained, higher doses (generally 25 mg/kg/day taken orally, or greater), there is increased risk of several reversible toxicities, especially peripheral neuropathy, neurotoxicity, and gait disturbance. [5] [35] Studies have also shown that it can be carcinogenic in male B6C3F1 mice at high doses.[38]
Neuropathy A clinical trial where DCA was given to patients of MELAS (a form of genetically inherited lactic acidosis) at 25 mg/kg/day was ended prematurely due to excessive peripheral nerve toxicity.[39] Dichloroacetate can also have anxiolytic or sedative effects.[4] Animal studies suggest that the neuropathy and neurotoxicity during chronic dichloroacetate treatment may be partly due to depletion of thiamine, and thiamine supplementation in rats reduced these effects.[40] However, more recent studies in humans suggest that peripheral neuropathy is a common side effect during chronic DCA treatment, even with coadministration of oral thiamine. [41] [42] An additional study reported that 50 mg/kg/day DCA treatment resulted in unsteady gait and lethargy in two patients, with symptoms occurring after one month for one patient and two months for the second. Gait disturbance and consciousness were recovered with cessation of DCA, however sensory nerve action potentials did not recover in one month.[43] It has been reported that animals and patients treated with DCA have elevated levels of delta- aminolevulinic acid (delta-ALA) in the urine. A study published in 2008 suggests that this product may be the cause of the neurotoxic side effect of DCA by blocking peripheral myelin formation.[44]
Carcinogenicity Long term use (three years or more) of high doses (> 77 mg/kg/day) of DCA has been shown to increase risk of liver cancer in mice.[38] Studies of the trichloroethylene (TCE) metabolites dichloroacetic acid (DCA), trichloroacetic acid (TCA), and chloral hydrate suggest that both DCA and TCA are involved in TCE-induced liver tumorigenesis and that many DCA effects are consistent with conditions that increase the risk of liver cancer in humans.[45] It should be noted here that the maximum recommended dose for cancer treatment is 20mg/kg/day (less than 1/3rd of the 77mg/kg/day shown to increase liver cancer risk in mice). Self-medication The promise of DCA as a cheap, effective and safe treatment for cancer generated a great deal of public interest. Many people turned to self-medication. [46] [47] Doctors warned of potential problems if people attempt to try DCA outside a controlled clinical trial. "If it starts going badly, who is following you before it gets out of control? By the time you realize your liver is failing, you're in big trouble", said Laura Shanner, Associate Professor of Health Ethics at the University of Alberta.[48]
References 1. ^ a b c d Haynes, William M., ed. (2011). CRC Handbook of Chemistry and Physics (92nd ed.). CRC Press. ISBN 1439855110. 2. ^ "Dichloracetate (DCA)". American Cancer Society. Retrieved 1 December 2012. 3. ^ J.T. Baker MSDS 4. ^ a b Stacpoole P, Henderson G, Yan Z, James M (1998). "Clinical pharmacology and toxicology of dichloroacetate". Environ Health Perspect. 106 Suppl 4: 989–994. doi:10.2307/3434142. JSTOR 3434142. PMC 1533324. PMID 9703483. 5. ^ a b Stacpoole PW (1989). "The pharmacology of dichloroacetate". Metabolism 38 (11): 1124–1144. doi:10.1016/0026-0495(89)90051-6. PMID 2554095. 6. ^ Stacpoole P, Lorenz A, Thomas R, Harman E (1988). "Dichloroacetate in the treatment of lactic acidosis". Ann Intern Med 108 (1): 58–63. PMID 3337517. 7. ^ a b Stacpoole P, Kerr D, Barnes C, Bunch S, Carney P, Fennell E, Felitsyn N, Gilmore R, Greer M, Henderson G, Hutson A, Neiberger R, O'Brien R, Perkins L, Quisling R, Shroads A, Shuster J, Silverstein J, Theriaque D, Valenstein E (2006). "Controlled clinical trial of dichloroacetate for treatment of congenital lactic acidosis in children". Pediatrics 117 (5): 1519–1531. doi:10.1542/peds.2005-1226. PMID 16651305. 8. ^ a b Kaufmann P, Engelstad K, Wei Y, Jhung S, Sano M, Shungu D, Millar W, Hong X, Gooch C, Mao X, Pascual J, Hirano M, Stacpoole P, DiMauro S, De Vivo D (2006). "Dichloroacetate causes toxic neuropathy in MELAS: a randomized, controlled clinical trial". Neurology 66 (3): 324–330. doi:10.1212/01.wnl.0000196641.05913.27. PMID 16476929. 9. ^ a b Stacpoole P, Wright E, Baumgartner T, Bersin R, Buchalter S, Curry S, Duncan C, Harman E, Henderson G, Jenkinson S (1992). "A controlled clinical trial of dichloroacetate for treatment of lactic acidosis in adults. The Dichloroacetate-Lactic Acidosis Study Group". N Engl J Med 327 (22): 1564–1569. doi:10.1056/NEJM199211263272204. PMID 1435883. 10. ^ Xu R, Pelicano H, Zhou Y, Carew J, Feng L, Bhalla K, Keating M, Huang P (2005). "Inhibition of glycolysis in cancer cells: a novel strategy to overcome drug resistance associated with mitochondrial respiratory defect and hypoxia". Cancer Res 65 (2): 613–21. PMID 15695406. 11. ^ Kim JW, Dang CV (2006). "Cancer's molecular sweet tooth and the Warburg effect". Cancer Res. 66 (18): 8927–8930. doi:10.1158/0008-5472.CAN-06-1501. PMID 16982728. 12. ^ a b c "Cheap, ‘safe’ drug kills most cancers". New Scientist. 2007-01-17. Retrieved 2007-01-17. 13. ^ Bonnet, Sébastien; Archer, Stephen L.; Allalunis-Turner, Joan; Haromy, Alois; Beaulieu, Christian; Thompson, Richard; Lee, Christopher T.; Lopaschuk, Gary D. et al. (2007). "A Mitochondria-K+ Channel Axis Is Suppressed in Cancer and Its Normalization Promotes Apoptosis and Inhibits Cancer Growth". Cancer Cell 11 (1): 37–51. doi:10.1016/j.ccr.2006.10.020. PMID 17222789. 14. ^ a b c "DCA: Cancer Breakthrough or Urban Legend?" From ABC News, 5 February 2007. Accessed 15 February 2007. 15. ^ a b "No Wonder Drug", letter to New Scientist from Ralph Moss Lemont. Published February 3, 2007. Accessed 16 February 2007. 16. ^ a b c d Michelakis, E. D.; Sutendra, G.; Dromparis, P.; Webster, L.; Haromy, A.; Niven, E.; Maguire, C.; Gammer, T. L. et al. (2010). "Metabolic Modulation of Glioblastoma with Dichloroacetate". Sci Transl Med 2 (31): 31ra34–31ra34. doi:10.1126/scitranslmed.3000677. PMID 20463368. 17. ^ T. E. (Thomas Edward) Thorpe. A Dictionary of Applied Chemistry. Vol. 3. Page 9 of 189 at www.ebooksread.com/authors-eng/t-e-thomas-edward-thorpe/a-dictionary-of- applied-chemistry-volume-3-hci/page-9-a-dictionary-of-applied-chemistry-volume-3- hci.shtml 18. ^ "Editorial: Gambling with your life", New Scientist, 31 March 2007 19. ^ a b Shahrzad, Siranoush; Lacombe, Kristen; Adamcic, Una; Minhas, Kanwal; Coomber, Brenda L. (November 2010). "Sodium dichloroacetate (DCA) reduces apoptosis in colorectal tumor hypoxia". Cancer Letters 297 (1): 75–83. doi:10.1016/j.canlet.2010.04.027. PMID 20537792. 20. ^ a b "CTV.ca: Researchers launch website on new cancer research". CTV News. 21. ^ "CTV.ca: Small molecule offers hope for cancer treatment". CTV News. Retrieved 21 April 2012. 22. ^ "Small molecule offers big hope against cancer", by Ryan Smith. From ExpressNews, a University of Alberta publication. Published January 16, 2007. Accessed 15 February 2007. 23. ^ Official University of Alberta DCA Site 24. ^ A Method of Treating Cancer Using Dichloroacetate, Application to the European Patent Office, 19 October 2006 25. ^ http://www.dca.med.ualberta.ca/Home/Updates/letter_092407.pdf, 24 September 2007 26. ^ The Safety and Efficacy of DCA for the Treatment of Brain Cancer, ClinicalTrials.gov identifier: NCT00540176 27. ^ Outlook 2008, Tufts Center for the Study of Drug Development 28. ^ Michelakis, ED; Sutendra, G; Dromparis, P; Webster, L; Haromy, A; Niven, E; Maguire, C; Gammer, TL et al. (2010). "Metabolic modulation of glioblastoma with dichloroacetate". Science translational medicine 2 (31): 31ra34–31ra34. doi:10.1126/scitranslmed.3000677. PMID 20463368. 29. ^ The Cure for Cancer Has Been Found and is Purposely Being Ignored - Technorati blog (accessed 16/05/2011) 30. ^ DCA Research Team publishes results of Clinical Trials - University of Alberta website 31. ^ Potential cancer drug DCA tested in early trials - Cancer Research UK science blog 32. ^ @CR_UK tweet - tweeted 16/05/11 33. ^ Cancer drug resurfaces and threatens false optimism - New Scientist, 16 May 2011 34. ^ US 8071645, Newell, M. Karen; Newell, Evan & Villalobos-Menuey, Elizabeth, "Systems and methods for treating human inflammatory and proliferative diseases and wounds, with fatty acid metabolism inhibitors and/or glycolytic inhibitors" 35. ^ a b Picard, André (2007-01-17). "Long-used drug shows new promise for cancer". Toronto: The Globe and Mail. Retrieved 2007-01-17. 36. ^ Pearson H; Kurtz, TL; Han, Z; Langaee, T (2008). "Role of dichloroacetate in the treatment of genetic mitochondrial diseases". Adv Drug Deliv Rev. 60 (13,14): 1478–1487. doi:10.1016/j.addr.2008.02.014. PMID 18647626. 37. ^ Agbenyega T, Planche T, Bedu-Addo G, Ansong D, Owusu-Ofori A, Bhattaram VA, Nagaraja NV, Shroads AL, Henderson GN, Hutson AD, Derendorf H, Krishna S, Stacpoole PW (2003). "Population kinetics, efficacy, and safety of dichloroacetate for lactic acidosis due to severe malaria in children". J Clin Pharmacol. 43 (4): 386–396. doi:10.1177/0091270003251392. PMID 12723459. 38. ^ a b DeAngelo AB, Daniel FB, Stober JA, Olson GR (1991). "The carcinogenicity of dichloroacetic acid in the male B6C3F1 mouse". Fundam Appl Toxicol. 16 (2): 337–347. doi:10.1016/0272-0590(91)90118-N. PMID 2055364. 39. ^ Kaufmann P, Engelstad K, Wei Y et al. (2006). "Dichloroacetate causes toxic neuropathy in MELAS: a randomized, controlled clinical trial". Neurology 66 (3): 324–330. doi:10.1212/01.wnl.0000196641.05913.27. PMID 16476929. 40. ^ Stacpoole P, Harwood H, Cameron D, Curry S, Samuelson D, Cornwell P, Sauberlich H (1990). "Chronic toxicity of dichloroacetate: possible relation to thiamine deficiency in rats". Fundam Appl Toxicol 14 (2): 327–37. doi:10.1016/0272-0590(90)90212- 3. PMID 2318357. 41. ^ Kurlemann G, Paetzke I, Moller H, Masur H, Schuierer G, Weglage J, Koch HG (1995). "Therapy of complex I deficiency: peripheral neuropathy during dichloroacetate therapy". Eur J Pediatr 154 (11): 928–32. doi:10.1007/BF01957508. PMID 8582409. 42. ^ Spruijt L, Naviaux RK, McGowan KA, Nyhan WL, Sheean G, Haas RH, Barshop BA (2001). "Nerve conduction changes in patients with mitochondrial diseases treated with dichloroacetate". Muscle Nerve 24 (7): 916–24. doi:10.1002/mus.1089. PMID 11410919. 43. ^ Oishi K, Yoshioka M, Ozawa R, Yamamoto T, Oya Y, Ogawa M, Kawai M (2003). "Dichloroacetate treatment for adult patients with mitochondrial disease". Rinsho Shinkeigaku 43 (4): 154–61. PMID 12892050. 44. ^ Felitsyn, N; McLeod, C; Shroads, AL; Stacpoole, PW; Notterpek, L (2008). "The heme precursor delta-aminolevulinate blocks peripheral myelin formation". Journal of Neurochemistry 106 (5): 2068–2079. doi:10.1111/j.1471-4159.2008.05552.x. PMC 2574579. PMID 18665889. 45. ^ Caldwell JC, Keshava N (September 2006). "Key issues in the modes of action and effects of trichloroethylene metabolites for liver and kidney tumorigenesis". Environ. Health Perspect. 114 (9): 1457–63. PMC 1570066. PMID 16966105. 46. ^ Pearson, Helen (2007). "Cancer patients opt for unapproved drug". Nature 446 (7135): 474–475. doi:10.1038/446474a. PMID 17392750. 47. ^ Interview: Would you try an untested cancer drug?, New Scientist, August 15, 2007 48. ^ Andrea Sands (March 18, 2007). "Experts caution against patients compiling own data on unapproved cancer drug". Edmonton Journal.
External links • International Chemical Safety Card 0868 • CTV.ca News Staff (16 January 2007). "Small molecule offers hope for cancer treatment". CTV.ca Website (CTV television network). Retrieved 2007-01-31. • DCA Research Information Website (University of Alberta) • Wait for Clinical Trials , New Scientist, 24 February 2007 • Potential cancer drug DCA tested in early trials , by Cancer Research UK • Interviewing Drs. Akbar and Humaira Khan about DCA • Cancer Biology – Cramping Tumors Economist, January 18, 2007 • Official University of Alberta DCA (dichloroacetate) Website , The University of Alberta Discovery. March 15, 2007