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WARS Antibody Cat. No.: 27-187 WARS Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human WARS. TESTED APPLICATIONS: ELISA, WB WARS antibody can be used for detection of WARS by ELISA at 1:62500. WARS antibody APPLICATIONS: can be used for detection of WARS by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate PREDICTED MOLECULAR 52 kDa WEIGHT: Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid September 28, 2021 1 https://www.prosci-inc.com/wars-antibody-27-187.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store WARS STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: WARS ALTERNATE NAMES: WARS, GAMMA-2, IFI53, IFP53 ACCESSION NO.: NP_004175 PROTEIN GI NO.: 47419914 GENE ID: 7453 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by BACKGROUND: their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. REFERENCES: 1) Shen, N., (2008) Nucleic Acids Res. 36 (4), 1288-1299. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 28, 2021 2 https://www.prosci-inc.com/wars-antibody-27-187.html.

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WARS Recombinant Protein Description Product Info
9853 Pacific Heights Blvd. Suite D. San Diego, CA 92121, USA Tel: 858-263-4982 Email: [email protected] 32-2929: WARS Recombinant Protein Alternative GAMMA-2,IFI53,IFP53,WRS,WARS,TrpRS,hWRS,EC=6.1.1.2,Tryptophanyl-tRNA synthetase,Interferon- Name : induced protein 53,Tryptophan--tRNA ligase,GAMMA-2. Description Source : Escherichia Coli. WARS Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 491 amino acids (1-471 a.a.) and having a molecular mass of 55.3 kDa. The WARS is fused to a 20 amino acid His- Tag at N-terminus and purified by proprietary chromatographic techniques. WARS is part of the class I tRNA synthetase family. 2 types of tryptophanyl tRNA synthetase exist, a cytoplasmic form, called WARS, and a mitochondrial form, called WARS2. WARS catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. WARS controls ERK, Akt, and eNOS activation pathways that are related with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Product Info Amount : 25 µg Purification : Greater than 90.0% as determined by SDS-PAGE. 1mg/ml solution containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl, 1mM DTT & 10% Content : glycerol. Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of Storage condition : time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. Amino Acid : MGSSHHHHHH SSGLVPRGSH MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK EVTDEIVKEF MTPRKLSFDF Q.
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DNA Sequencing and Sorting: Identifying Genetic Variations
BioMath DNA Sequencing and Sorting: Identifying Genetic Variations Student Edition Funded by the National Science Foundation, Proposal No. ESI-06-28091 This material was prepared with the support of the National Science Foundation. However, any opinions, findings, conclusions, and/or recommendations herein are those of the authors and do not necessarily reflect the views of the NSF. At the time of publishing, all included URLs were checked and active. We make every effort to make sure all links stay active, but we cannot make any guaranties that they will remain so. If you find a URL that is inactive, please inform us at [email protected]. DIMACS Published by COMAP, Inc. in conjunction with DIMACS, Rutgers University. ©2015 COMAP, Inc. Printed in the U.S.A. COMAP, Inc. 175 Middlesex Turnpike, Suite 3B Bedford, MA 01730 www.comap.com ISBN: 1 933223 71 5 Front Cover Photograph: EPA GULF BREEZE LABORATORY, PATHO-BIOLOGY LAB. LINDA SHARP ASSISTANT This work is in the public domain in the United States because it is a work prepared by an officer or employee of the United States Government as part of that person’s official duties. DNA Sequencing and Sorting: Identifying Genetic Variations Overview Each of the cells in your body contains a copy of your genetic inheritance, your DNA which has been passed down to you, one half from your biological mother and one half from your biological father. This DNA determines physical features, like eye color and hair color, and can determine susceptibility to medical conditions like hypertension, heart disease, diabetes, and cancer.
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The Structure, Stability and Pheromone Binding of the Male Mouse Protein Sex Pheromone Darcin
The Structure, Stability and Pheromone Binding of the Male Mouse Protein Sex Pheromone Darcin Marie M. Phelan1, Lynn McLean2, Stuart D. Armstrong2, Jane L. Hurst3, Robert J. Beynon2, Lu-Yun Lian1* 1 NMR Centre for Structural Biology, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom, 2 Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom, 3 Mammalian Behaviour & Evolution Group, Institute of Integrative Biology, University of Liverpool, Leahurst Campus, Neston, United Kingdom Abstract Mouse urine contains highly polymorphic major urinary proteins that have multiple functions in scent communication through their abilities to bind, transport and release hydrophobic volatile pheromones. The mouse genome encodes for about 20 of these proteins and are classified, based on amino acid sequence similarity and tissue expression patterns, as either central or peripheral major urinary proteins. Darcin is a male specific peripheral major urinary protein and is distinctive in its role in inherent female attraction. A comparison of the structure and biophysical properties of darcin with MUP11, which belongs to the central class, highlights similarity in the overall structure between the two proteins. The thermodynamic stability, however, differs between the two proteins, with darcin being much more stable. Furthermore, the affinity of a small pheromone mimetic is higher for darcin, although darcin is more discriminatory, being unable to bind bulkier ligands. These attributes are due to the hydrophobic ligand binding cavity of darcin being smaller, caused by the presence of larger amino acid side chains. Thus, the physical and chemical characteristics of the binding cavity, together with its extreme stability, are consistent with darcin being able to exert its function after release into the environment.
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[One Step]WARS Antibody Kit
Leader in Biomolecular Solutions for Life Science [One Step]WARS Antibody Kit Catalog No.: RK05722 Basic Information Background Catalog No. Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate RK05722 amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first Applications proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a WB cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with Cross-Reactivity tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the Human, Mouse, Rat class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. Observed MW 53-55kDa Calculated MW 48kDa/53kDa Category Antibody kit Product Information Component & Recommended Dilutions Source Catalog No. Product Name Dilutions Rabbit RK05722-1 WARS Rabbit pAb 1:1000 dilution Purification RK05722-2 HRP Goat Anti-Rabbit IgG (H+L) 1:10000 dilution Affinity purification Storage Store at -20℃. Avoid freeze / thaw Immunogen Information cycles. Buffer: PBS with 0.02% sodium azide, Gene ID Swiss Prot 50% glycerol, pH7.3. 7453 P23381 Avoid repeated freeze-thaw cycles. Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 Contact of human WARS (NP_776049.1). www.abclonal.com Synonyms WARS;GAMMA-2;IFI53;IFP53 Validation Data Western blot analysis of extracts of various cells, using WARS antibody (A5758) at 1:1000 dilution ratio through one-step method. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only.
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Tryptophanyl Trna Synthetase (WARS) (NM 004184) Human Tagged ORF Clone Product Data
OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC203587L4 Tryptophanyl tRNA synthetase (WARS) (NM_004184) Human Tagged ORF Clone Product data: Product Type: Expression Plasmids Product Name: Tryptophanyl tRNA synthetase (WARS) (NM_004184) Human Tagged ORF Clone Tag: mGFP Symbol: WARS1 Synonyms: GAMMA-2; HMN9; IFI53; IFP53; WARS Vector: pLenti-C-mGFP-P2A-Puro (PS100093) E. coli Selection: Chloramphenicol (34 ug/mL) Cell Selection: Puromycin ORF Nucleotide The ORF insert of this clone is exactly the same as(RC203587). Sequence: Restriction Sites: SgfI-MluI Cloning Scheme: ACCN: NM_004184 ORF Size: 1413 bp This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 Tryptophanyl tRNA synthetase (WARS) (NM_004184) Human Tagged ORF Clone – RC203587L4 OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_004184.3 RefSeq Size: 2884 bp RefSeq ORF: 1416 bp Locus ID: 7453 UniProt ID: P23381, A0A024R6K8 Domains: WHEP-TRS, tRNA-synt_1b Protein Families: Druggable Genome Protein Pathways: Aminoacyl-tRNA biosynthesis, Tryptophan metabolism MW: 53.2 kDa Gene Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid.
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Bioinformatics: a Practical Guide to the Analysis of Genes and Proteins, Second Edition Andreas D
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Β1 Integrins Are Required for Normal CNS Myelination and Promote AKT-Dependent Myelin Outgrowth Claudia S
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Global Analysis of Trna and Translation Factor Expression Reveals a Dynamic Landscape of Translational Regulation in Human Cancers
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