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i EMERGING THEMES IN FUNDAMENTAL AND APPLIED SCIENCES Edited by Rosimah Nulit Faridah Qamaruz Zaman Hishamuddin Omar Yap Chee Kong Faculty of Science UPM Press Universiti Putra Malaysia Universiti Putra Malaysia Serdang Serdang Selangor Selangor Malaysia Malaysia ii Volume Pertama /First volume 2018 Hak Cipta Universiti Putra Malaysia/ Copyright Universiti Putra Malaysia, 2018 Universiti Putra Malaysia Hak cipta terpelihara. Tiada bahagian daripada buku ini boleh diterbitkan semula, disimpan untuk pengeluaran atau ditukarkan ke dalam sebarang bentuk atau dengan sebarang alat, sama ada dengan cara elektonik, gambar serta rakaman dan sebagainya tanpa kebenaran bertulis daripada Penerbit UPM terlebih dahulu All right reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical including photocopy, recording, or any information storage and retrieval system, without permission in writing from UPM Press Diterbitkan di Malaysia oleh/Published in Malaysia by Penerbit UPM Universiti Putra Malaysia 43400 UPM, Serdang Selangor Hakcipta terpelihara: www.science.upm.edu.my/ebook-3213 Perpustakaan Negara Malaysia Data pengkatalogan-dalam- Penerbitan Emerging Themes in Fundamental and Applied Sciences Editor: Rosimah Nulit, Faridah Qamaruz zaman, Hishamuddin Omar, Yap Chee Kong e-ISBN 978-967-344-829-6 iii CONTENTS PREFACE v CONTRIBUTORS vi CHAPTER 1 INTRODUCTION 1 CHAPTER 2 BIODIVERSITY AND NATURAL RESOURCES 3 2.1 Diversity Of Mixed Microalgae In Catfish Tanks Under Different Weather Conditions 2.2 Physico-Chemical Properties And Amplification Of 16s rDNA From An Acidic Lake, Puchong 2.3 Lichens And The Environment: Preliminary Study On Fraser’s Hill, Malaysia 2.4 Diversity of invertebrates in different agricultural of soils 2.5 Anuran Species Diversity And Distribution At Compartment 12 In Ayer Hitam Forest Reserve, Puchong, Selangor 2.6 Relationship Between Water Quality And Diversity Of Anurans In Compartment 12, Ayer Hitam Forest Reserve, Puchong 2.7 Fish Fauna Composition and Water Quality at Long Kesseh, Baram, Sarawak 2.8 Antioxidant activity of Cymodocea rotundata from various habitats 2.9 Nutrient content of wild pepper, Piper umbellatum from Sg. Asap Koyan, Belaga, Sarawak 2.10 Morphological Characteristics of Wild Pepper, Piper umbellatum L. from Belaga, Sarawak 2.11 Preliminary Phytochemical Screening Of Chrysanthemum morifolium Under Different Extraction Methods CHAPTER 3 ECOLOGY 82 3.1 Comparison of Length-Weight Relationship of Cyclocheilichthys apogon (Valenciennes, 1842) between Tail Waters of Bakun Hydroelectric Dam and Batang Ai Hydroelectric Dam 3.2 Threats and Conservation of Tropical Peat Swamp Forests ii 3.3 Fire Threat in Peat Swamp Forest in Malaysia 3.4 Biological Effects of Tributyltin Chloride (TBTCl) on Juveniles Stage of Life cycle Artemia salina 3.6 Bioaccumulation of lead and cadmium in catfish tissues (Arius thalassinus) from Kuala Gula, Perak, Peninsular Malaysia 3.7 Soil Morphological Properties in Acacia mangium Plantation Area, Bintulu, Sarawak 3.8 Optimal method to introduce faeces sample for olfactory-cues studies in Malayan tapir (Tapirus indicus) CHAPTER 4 ANIMAL PHYSIOLOGY 137 4.1 Reproductive Status of Bats Species in Compartment 15, Ayer Hitam Forest Reserve, Puchong, Selangor 4.2 Reproductive Pattern of Cynopterus brachyotis from Secondary Forest in Ayer Hitam Forest Reserve, Puchong, Selangor 4.3 A Review on Polyamine Analogues and Its Cytotoxicity Effect Against Selected Human Cancer Cell Lines 4.4 Distribution Pattern of Brucella melitensis in Organs of Bucks Following Experimental Acute Brucellosis 4.5 Effect of Minerals on β-galactosidase activity of Lactobacillus fermentum Isolated from Raw Cow Milk 4.6 Extremely Low Electromagnetic Field (1.0 mT, 50 Hz) Exposure Effects Osteoblasts Growth on Nanobiocomposite Bone Scaffold 4.7 Design and evaluation of PCR primer and protocol for progesterone receptor in ovaries tissue of female goats 4.8 Evaluation of Magnetized Water (MW) on the Growth Performance and Survival of Hybrid Red Tilapia Oreochromis spp. 4.9 LCMS/MS Detection of Faecal progestogens in Female Malayan Tapir iii CHAPTER 5 PLANT PHYSIOLOGY 212 5.1 Impact Of Potassium Fertilizaton On The Growth And Secondary Metabolites Of Orthosiphon stamineus (Misai Kucing) 5.2 Carbon Assimilation, Leaf Gas Exchange And Quality Of Ipomea aquatica As Affected By Nitrogen Rates 5.3 Morphological, physicochemical and functional properties of cattail (Typha angustifolia L.) starch 5.4 Morphological changes and nutrients enrichment on Halophila ovalis (R.Br.) Hooker f. and Halophila beccarii Aschers (Hydrocharitaceae) under laboratory condition 5.5 Lasiodiplodia theobromae, L. pseudotheobromae and Pseudofusicoccum adansoniae causing fruit rot disease of commercial fruits 5.6 Screening of potential antagonistic Trichoderma species from soil against Fusarium oxysporum f. sp. Lycopersici 5.7 Spatial distribution of Bacterial Heart Rot (BHR) disease of pineapple 5.8 Influence of lead on seed germination and plant growth of Acacia auriculiformis species iv PREFACE This book is the first volume of research papers presented at the Fundamental Science Congress 2017 (FSC 2017). The Congress was held by the Faculty of Science on November 21-22, 2017. In the first volume, thirty-six (36) manuscripts had been received from participants and are categorized into four subfields in biological sciences. Chapter 2 presented the biodiversity and natural resources. The diversity of microorganism to algae, invertebrate and vertebrate from different ecosystems is discussed. The natural resources in Malaysia, especially from Sabah and Sarawak and the benefits of these natural resources on mankind also discovered. Chapter 3 contains the multidisciplinary field in ecology, from freshwater fish, the ecotoxicity of fish and brimp shrimp. The relationship between plant and soil also discussed. Chapter 4 comprises studies on animal physiology. The reproductive status and pattern of bats, the potential compounds as anti-cancer agents, and causative agents of brucellosis in animals was studied. Chapter 5 presented research works on the growth, physiology, morphology of selected herb, vegetable, and seagrasses. Study on the pathogens from fruits and the potential antagonistic Trichoderma isolates against Fusarium oxysporum f. sp. lycopersici condition is presented. Epidemiology study on the spread and management strategies for Bacterial heart rot (BHR) disease also discussed in this chapter. The potential of Acacia auriculiformis as phytoremediator of lead was presented. We would like to express our thank you to all authors for articles contribution. It is hoped that the book will be benefit to other. R. Nulit v CONTRIBUTORS PREFACE Rosimah Nulit Dept. of Biology Faculty of Science Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Email:[email protected] CHAPTER 1 INTRODUCTION Rosimah Nulit*, Faridah Qamaruz Zaman, Hishamuddin Omar, Yap Chee Kong Dept. of Biology Faculty of Science Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Email:[email protected] CHAPTER 2 BIODIVERSITY 2.1 M. Weeraphong1, N.B.M. Yusoff1, H. Omar1, S.Z.B. Zulkifli1, M.N.A.B. Azmai1, and A. Ismail1* Dept. of Biology Faculty of Science Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Email: [email protected] 2.2 N. N. F. Mat Lazim1 and S. Zulkifly1,2 1Dept. of Biology Faculty of Science Universiti Putra Malaysia 43400 UPM Serdang, Selangor 2Institut Penyelidikan Matematik Universiti Putra Malaysia 43400 UPM Serdang, Selangor Email: [email protected] 2.3 S. Rohadi and S. Zulkifly Dept. of Biology Faculty of Science Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Email: [email protected] vii 2.4 A. Maqtan, H. Omar, M. Mustafa, N.A. Izzati, A. Ismail1*, D. S. Karam2 1 Dept. of Biology Faculty of Science Universiti Putra Malaysia 43400 UPM, Serdang, Selangor, Malaysia 2 Dept. of Land Management Faculty of Agriculture Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Email: [email protected] 2.5 Nadia Simon1, Nadirah Rosli3, Siti Fara Najua Mohd. Nasir1, Muhammad Faris Abdul Aziz1, Mohamad Azani Alias2, Mohamad Roslan Mohamad Kasim3, Marina Mohd. Top @ Mohd. Tah1,2 1 Dept. of Biology Faculty of Science Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia 2Centre of Foundation Studies for Agricultural Science Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia 3 Dept. of Forest Management Faculty of Forestry Universiti Putra Malaysia 43400 UPM Serdang, Selangor Email: [email protected] 2.6 Nadirah Rosli1, Nadia Simon2, Siti Fara Najua Mohd. Nasir2, Muhammad Faris Abdul Aziz2, Mohamad Azani Alias1, Mohamad Roslan Mohamad Kasim1, Marina Mohd. Top @ Mohd. Tah2,3 1Dept. of Forest Management Faculty of Forestry Universiti Putra Malaysia 43400 UPM Serdang, Selangor 2Dept. of Biology Faculty of Science Universiti Putra Malaysia 43400 UPM Serdang, Selangor 3Centre of Foundation Studies for Agricultural Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Email: [email protected] vii 2.7 S. Juliana1*, L. Nyanti1 and G. Jongkar2 1Aquatic Science and Resource Management Program Faculty of Resource Science and Technology Universiti Malaysia Sarawak 94300 Kota Samarahan, Sarawak, Malaysia 2Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak, 94300 Kota Samarahan,Sarawak, Malaysia Email: [email protected] 2.8 L.S.H. Emmclan1, Z. Muta Harah2 and