Anti-ACADSB Antibody (ARG55455)

Product datasheet [email protected]

ARG55455 Package: 100 μl anti-ACADSB antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes ACADSB

Tested Reactivity Hu

Tested Application WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name ACADSB

Antigen Species Human

Immunogen KLH-conjugated synthetic peptide corresponding to aa. 239-273 (Center) of Human ACADSB.

Conjugation Un-conjugated

Alternate Names Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; SBCAD; ACAD7; 2-methylbutyryl- coenzyme A dehydrogenase; EC 1.3.8.5; 2-methylbutyryl-CoA dehydrogenase; 2-methyl branched chain acyl-CoA dehydrogenase; 2-MEBCAD

Application Instructions

Application table Application Dilution

WB 1:1000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control A431

Calculated Mw 47 kDa

Properties

Form Liquid

Purification Purification with Protein A and immunogen peptide.

Buffer PBS and 0.09% (W/V) Sodium azide

Preservative 0.09% (W/V) Sodium azide

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

www.arigobio.com 1/2 Bioinformation

Database links GeneID: 36 Human

Swiss-port # P45954 Human

Gene Symbol ACADSB

Gene Full Name acyl-CoA dehydrogenase, short/branched chain

Background Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul 2008]

Function Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent. [UniProt]

Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Cellular Localization Mitochondrion matrix.

Images

ARG55455 anti-ACADSB antibody WB image

Western blot: 35 µg of A431 cell lysate stained with ARG55455 anti- ACADSB antibody at 1:1000 dilution.