J_J0004 1

BIOACTIVE COMPOUNDS FROM jasminoides ELLIS PARTS OTHER THAN FRUIT Sarana Sommano ,1,* Mayuree Chompoo,1 Korawan Sri-agarm 2 1Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand 2Central Laboratory, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand *e-mail: [email protected]

Abstract: Fruit of gardenia ( Gardenia jasminoides Ellis) or fructus has been recorded as traditional Chinese medicine (TCM) for many years due to its bitter and cold properties which has been used for the remedies of hepatic pain, abdominal pain and is used as anti- phlogistics, diuretic, laxative, choleretic, and homeostatic purposes in the treatment of trauma by external application. Even though G. jasminoides is grown commercially for flower production, there is no evidence of gardenia fruit in Thailand. This research investigates bioactive compounds from leaf shoot, flower as well as essential oil in comparison to fruit of G. jasminoides . Thin layer chromatography (TLC) was utilized in order to distinguish compounds from dichloromethane and methanol extracts. The freshly harvested flower was also identified for volatile compounds by solid-phase microextraction (SPME) at ambient temperature. Results suggested that floral and leaf tissue contained substantial amount of antioxidants from the two kinds of extracts as compared to fruit. The SPME profile suggested (Fig. 1) that cis-3-hexenly tiglate was a major compound found in fresh gardenia flower, followed by methyl benzoate and linalool. The outcome of this research may assist the development of functional food and/or food supplement from this crop. Abundance

TIC: 55-082_1.D\data.ms 11.55411.690 18.08918.156 22.667 Methyl benzoate Linalool Cis-3hexyl triglate 4000000 10.112

3500000

3000000

2500000

22.379 2000000 20.444

22.258 1500000 17.20919.410 21.159

24.735 1000000 15.693 24.067 15.397 5.185 20.610 500000 26.306 14.37314.463 19.836 23.03224.225

5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 Time--> Fig. 1 SPME profile of G. jasminoides flower

References: 1. Chaichana J, Niwatananun W, Vejabhikul S, Somna S, Chansakaow S. J Health Sci 2009;23:141-145. 2. Debnath T, Park P-J, Deb Nath NC, Samad NB, Park HW, Lim BO. Food Chem 2011;128:697-703.

Keywords: cape , solid-phase microextraction, thin layer chromatography

© 38th Congress on Science and Technology of Thailand (Abstract)