DOCSLIB.ORG

Journal of Bacteriology

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

JOURNAL OF BACTERIOLOGY Volume 169 June 1987 No. 6 STRUCTURE AND FUNCTION Assembly of a Chemically Synthesized Peptide of Escherichia coli Type 1 Fimbriae into Fimbria-Like Antigenic Structures. Soman N. Abraham and Edwin H. Beachey ....... 2460-2465 Structure of the Staphylococcus aureus Cell Wall Determined by the Freeze- Substitution Method. Akiko Umeda, Yuji Ueki, and Kazunobu Amako ... 2482-2487 Labeling of Binding Sites for 02-Microglobulin (02m) on Nonfibrillar Surface Structures of Mutans Streptococci by Immunogold and I21m-Gold Electron Microscopy. Dan Ericson, Richard P. Ellen, and Ilze Buivids ........... 2507-2515 Bicarbonate and Potassium Regulation of the Shape of Streptococcus mutans NCTC 10449S. Lin Tao, Jason M. Tanzer, and T. J. MacAlister......... 2543-2547 Periodic Synthesis of Phospholipids during the Caulobacter crescentus Cell Cycle. Edward A. O'Neill and Robert A. Bender.............................. 2618-2623 Association of Thioredeoxin with the Inner Membrane and Adhesion Sites in Escherichia coli. M. E. Bayer, M. H. Bayer, C. A. Lunn, and V. Pigiet 2659-2666 Cell Wall and Lipid Composition of Isosphaera pallida, a Budding Eubacterium from Hot Springs. S. J. Giovannoni, Walter Godchaux III, E. Schabtach, and R. W. Castenholz.............................................. 2702-2707 Charge Distribution on the S Layer of Bacillus stearothermophilus NRS 1536/3c and Importance of Charged Groups for Morphogenesis and Function. Margit Saira and Uwe B. Sleytr ....................................... 2804-2809 PLANT MICROBIOLOGY Rhizobium meliloti ntrA (rpoN) Gene Is Required for Diverse Metabolic Functions. Clive W. Ronson, B. Tracy Nixon, Lisa M. Albright, and Frederick M. Ausubel............................................... 2424-2431 Bradyrhizobium japonicum Mutants Defective in Nitrogen Fixation and Molybde- num Metabolism. Robert J. Maier, Lennox Graham, Robert G. Keefe, Todd Pihl, and Eric Smith............................................ 2548-2554 Variability in Molybdenum Uptake Activity in Bradyrhizobium japonicum Strains. Lennox Graham and Robert J. Maier .................................. 2555-2560 A Locus Encoding Host Range Is Linked to the Common Nodulation Genes of Bradyrhizobium japonicum. Anthony J. Nieuwkoop, Zsofia Banfalvi, Nirupama Deshmane, David Gerhold, Maria G. Schell, Karl M. Sirotkin, and Gary Stacey.................................................... 2631-2638 Inhibition of Hydrogenase Synthesis by DNA Gyrase Inhibitors in Bradyrhizobium japonicum. Patricia D. Novak and Robert J. Maier ..................... 2708-2712 Flagella of a Plant-Growth-Stimulating Pseudomonasfluorescens Strain Are Re- quired for Colonization of Potato Roots. Letty A. De Weger, Cornelia I. M. van der Vlugt, Andre H. M. Wijfjes, Peter A. H. M. Bakker, Bob Schippers, and Ben Lugtenberg ....................................... 2769-2773 Nitrogen-Fixing Nodules Induced by Agrobacterium tumefaciens Harboring Rhizo- bium phaseoli Plasmids. Esperanza Martinez, Rafael Palacios, and Federico Sainchez................................................... 2828-2834 Continued on following page Continuedfrom preceding page GENETICS AND MOLECULAR BIOLOGY Gene Expression in Zymomonas mobilis: Promoter Structure and Identification of Membrane Anchor Sequences Forming Functional LacZ' Fusion Proteins. T. Conway, Y. A. Osman, and L. 0. Ingram ...... ..................... 2327-2335 Mutational Alterations Affecting the Export Competence of a Truncated but Fully Functional Maltose-Binding Protein Signal Peptide. John D. Fikes, Vytas A. Bankaitis, J. Patrick Ryan, and Philip J. Bassford, Jr . ................ 2345-2351 Export of Unprocessed Precursor Maltose-Binding Protein to the Periplasm of Escherichia coli Cells. John D. Fikes and Philip J. Bassford, Jr. .... ..... 2352-2359 Spectinomycin Resistance and Associated DNA Amplification in Streptomyces ach- romogenes subsp. rubradiris. Ulfert Hornemann, Christopher J. Otto, Guy G. Hoffman, and Anne C. Bertinuson .................................. 2360-2366 Photoreactivation in phr Mutants of Escherichia coli K-12. Intisar Husain and Aziz Sancar ......................................................... 2367-2372 Sequence Analysis of the 17-Kilodalton-Antigen Gene from Rickettsia rickettsii. Burt E. Anderson, Russell L. Regnery, George M. Carlone, Theodore Tzianabos, Joseph E. McDade, Zhang Yuan Fu, and William J. Bellini.... 2385-2390 The phs Gene and Hydrogen Sulfide Production by Salmonella typhimurium. Marta A. Clark and Ericka L. Barrett.................................. 2391-2397 Regulation of Expression of Pectate Lyase Genes pelA, pelD, and pelE in Erwinia chrysanthemi. Sylvie Reverchon and Janine Robert-Baudouy .... ........ 2417-2423 Selection of Mutations That Alter the Osmotic Control of Transcription of the Sal- monella typhimurium proU Operon. Janice Druger-Liotta, Virginia Joan Prange, David G. Overdier, and Laszlo N. Csonka ...................... 2449-2459 Creation of a Test Plasmid for Detecting G * C-to-T * A Transversions by Chang- ing Serine to Arginine in the Active Site of ,-Lactamase. Patricia L. Foster, Gloria Dalbadie-McFarland, Elaine F. Davis, Steve C. Schultz, and John H. Richards .................................................... 2476-2481 Regulatory Mutants of the aroF-tyrA Operon of Escherichia coli K-12. Christopher S. Cobbett and Margaret L. Delbridge ...... ................ 2500-2506 Characterization of the sppA Gene Coding for Protease IV, a Signal Peptide Pepti- dase of Escherichia coli. Tohru Suzuki, Akira Itoh, Shigeyuki Ichihara, and Shoji Mizushima ................................................. 2523-2528 Positive and Negative Regulation of the bgl Operon in Escherichia coli. Subramony Mahadevan, Ann E. Reynolds, and Andrew Wright .... ...... 2570-2578 ,-Glucoside (bgl) Operon of Escherichia coli K-12: Nucleotide Sequence, Genetic Organization, and Possible Evolutionary Relationship to Regulatory Compo- nents of Two Bacillus subtilis Genes. Karin Schnetz, Christian Toloczyki, and Bodo Rak ........................................................ 2579-2590 Cloning and Sequencing of the Alcohol Dehydrogenase II Gene from Zymomonas mobilis. T. Conway, G. W. Sewell, Y. A. Osman, and L. 0. Ingram ..... 2591-2597 Genetic Characterization of a Highly Efficient Alternate Pathway of Serine Bio- synthesis in Escherichia coli. Paula D. Ravnikar and Ronald L. Somerville 2611-2617 Operator Sequences of the Aerobactin Operon of Plasmid ColV-K30 Binding the Ferric Uptake Regulation (fur) Repressor. Victor de Lorenzo, Sechan Wee, Marta Herrero, and J. B. Neilands ............................... 2624-2630 Identification, Mapping, and Cloning of the Gene Encoding Cyanase in Escherich- ia coli K-12. Young-Chul Sung, Dawn Parsell, Paul M. Anderson, and James A. Fuchs ....................................................... 2639-2642 Isolation of Haemophilus influenzae Genes That Suppress Escherichia coli polA Mutations. Gene L. Williams, Brent Seaton, and David McCarthy ....... 2643-2649 Continued on following page Continuedfrom preceding page Nucleotide Sequence of a Gene Cluster Involved in Entry of E Colicins and Single- Stranded DNA of Infecting Filamentous Bacteriophages into Escherichia coli. Tai-Ping Sun and Robert E. Webster .................................. 2667-2674 Isolation and Characterization of Light-Regulated Phycobilisome Linker Polypep- tide Genes and Their Transcription as a Polycistronic mRNA. Terri L. Lomax, Pamela B. Conley, James Schilling, and Arthur R. Grossman..... 2675-2684 Expression and Regulation of a Vibrio alginolyticus Sucrose Utilization System Cloned in Escherichia coli. Renate R. Scholle, Vernon E. Coyne, Romilla Maharaj, Frank T. Robb, and David R. Woods ......................... 2685-2690 Cloning and Characterization of Elastase Genes from Pseudomonas aeruginosa. Peter A. Schad, Robert A. Bever, Thalia I. Nicas, Francois Leduc, Larry F. Hanne, and Barbara H. Iglewski............................. 2691-2696 Posttranscriptional Regulation of Ribosomal Protein S20 and Stability of the S20 mRNA Species. George A. Mackie ................................... 2697-2701 Suppressible Base Substitution Mutations Induced by Angelicin (Isopsoralen) in the Escherichia coli lacI Gene: Implications for the Mechanism of SOS Mutagenesis. Stephan S. Miller and Eric Eisenstadt .................... 2724-2729 Genetic Transformation in the Methanogen Methanococcus voltae PS. G. Bertani and L. Baresi ....................................................... 2730-2738 Molecular Cloning and Characterization of the recA Gene from the Cyanobacte- rium Synechococcus sp. Strain PCC 7002. Randy C. Murphy, Donald A. Bryant, Ronald D. Porter, and Nicole Tandeau de Marsac ............... 2739-2747 DNA Replication by a DNA-Membrane Complex Extracted from Bacillus subtilis: Site of Initiation In Vitro and Initiation Potential of Subcomplexes. John Laffan and William Firshein ......................................... 2819-2827 Promoter of the Pertussis Toxin Operon and Production of Pertussis Toxin. Alfredo Nicosia and Rino Rappuoli .................................... 2843-2846 Bordetella parapertussis and Bordetella bronchiseptica Contain Transcriptionally Silent Pertussis Toxin Genes. Beatrice Arico and Rino Rappuoli ......... 2847-2853 Genetic and Physical Analyses of a Cluster of Genes Essential for Xanthan Gum Biosynthesis in Xanthomonas campestris. Nancy E. Harding, Joseph M. Cleary, Debra K. Cabafias, Ira G. Rosen, and Kenneth S. Kang.......... 2854-2861
Recommended publications
  • A Gene Required for the Regulation of Photosynthetic Light Harvesting in the Cyanobacterium Synechocystis PCC6803

    A Gene Required for the Regulation of Photosynthetic Light Harvesting in the Cyanobacterium Synechocystis PCC6803

    A gene required for the regulation of photosyuthetic light harvesting in the cyanobacterium Synechocystis PCC6803 A thesis submitted for the degree of Doctor of Philosophy by Daniel Emlyn-Jones B.Sc. (Hons) Department of Biology University College London ProQuest Number: 10013938 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest. ProQuest 10013938 Published by ProQuest LLC(2016). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States Code. Microform Edition © ProQuest LLC. ProQuest LLC 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106-1346 THESIS ABSTRACT In cyanobacteria, state transitions serve to regulate the distribution of excitation energy delivered to the two photosystem reaction centres from the accessory light harvesting system, the phycobilisome. The trigger for state transitions is the redox state of the cytochrome b f complex/plastoquinone pool. The signal transduction events that connect this redox signal to changes in light harvesting are unknown. In order to identify signal transduction factors required for the state transition, random cartridge mutagenesis was employed in the cyanobacterium Synechocystis PCC6803 to generate a library of random, genetically tagged mutants. The state transition in cyanobacteria is accompanied by a change in fluorescence emission from PS2. By using a fluorescence video imaging system to observe this fluorescence change in mutant colonies it was possible to isolate mutants unable to perform state transitions.
  • Genetic Engineering of Avian Pathogenic E. Coli to Study the Functions of Fimh Adhesin

    Genetic Engineering of Avian Pathogenic E. Coli to Study the Functions of Fimh Adhesin

    Indian Journal of Experimental Biology Vol. 47, November 2009, pp. 916-920 Genetic engineering of avian pathogenic E. coli to study the functions of FimH adhesin H H Musa1,2§, S F He1§, S L Wu1, C H Zhu1, Z H Liu1, Z N Zhang1, V S Raj3, R X Gu 4 & G Q Zhu1* 1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China 2Faculty of Veterinary Science, University of Nyala, Nyala 155, Sudan 3Ranbaxy Laboratories Limited Research & Development Center, Gurgaon 122015, India 4Institute of Dairy Science and Technology, Yangzhou University, Yangzhou 225009, China Received 13 February 2009; revised 22 July 2009 Adhesion of pathogen to host cells is an important prerequisite for successful colonization and establishment of the pathogenesis. The aim of this study is to examine the function of FimH adhesin in the adherence of avian pathogenic E. coli to porcine intestinal epithelial cell lines (IPEC-J2) and human lung epithelial cell line (A549) in an in vitro infection model. Three strains of avian pathogenic Escherichia coli (APEC) and one strain of non-pathogenic E coli were used. The isogenic FimH mutants were constructed by λ Red-mediated recombination system. The wild types and mutants strains were adhered to the host cells with different adherence patterns in certain incubation time. The results demonstrated that the adherence of the isogenic FimH mutants to the porcine intestinal epithelial cells (IPEC-J2) were similar to those of wild types. However, the adherences of isogenic FimH mutants to human lung epithelial cells (A549) were significantly different from the wild types. A549 cell can be used as a type of cell model for colonization of the chicken extraintestinal.
  • Characterization of Cyanobacterial Phycobilisomes in Zwitterionic

    Characterization of Cyanobacterial Phycobilisomes in Zwitterionic

    Proc. Natl. Acad. Sci. USA Vol. 76, No. 12, pp. 6162-6166, December 1979 Biochemistry Characterization of cyanobacterial phycobilisomes in zwitterionic detergents (Synechococcus/photosynthetic accessory pigments/sedimentation/electron microscopy/aggregation) ALEXANDER N. GLAZER*, ROBLEY C. WILLIAMSt, GREGORY YAMANAKA*, AND H. K. SCHACHMANt *Department of Microbiology and Immunology, and tDepartment of Molecular Biology, University of California, Berkeley, California 94720 Contributed by Robley C. Williams, September 10, 1979 ABSTRACT Properties of cyanobacterial phycobilisomes preparations of nearly uniform-sized phycobilisomes were (from Synechococcus spp. 6301 and 6312 and Synechocystis sp. obtained. Ultrastructural studies of the particles prepared in 6701) prepared in the presence of two different zwitterionic detergents were compared to those of phycobilisomes detached zwitterionic detergents were facilitated by the marked decrease from membranes with the nonionic detergent Triton X-100 and in aggregation. Such studies show that phycobilisomes from then freed from Triton by sedimentation through high-salt su- different organisms have certain characteristics in common, crose density gradients. The absorption spectra, polypeptide as concluded by others (1, 7, 8), but also exhibit distinctive composition, and ultrastructure of phycobilisomes were inde- structural features. pendent of the detergent used during the preparation. Phyco- bilisomes from certain cyanobacteria aggregated in the absence MATERIALS AND METHODS of detergent. Such
  • Regulation of Iron-Uptake Systems in Vibrio Vulnificus, a Ferrophilic Bacterium

    Regulation of Iron-Uptake Systems in Vibrio Vulnificus, a Ferrophilic Bacterium

    Review Article Med Biol Sci Eng 2021;4(2):69-82 https://doi.org/10.30579/mbse.2021.4.2.69 pISSN 2586-5188ㆍeISSN 2586-5196 Regulation of iron-uptake systems in Vibrio vulnificus, a ferrophilic bacterium Sung-Heui Shin Department of Microbiology, College of Medicine, Chosun University, Gwangju, Korea Received March 22, 2021 Vibrio vulnificus is a gram-negative ferrophilic bacterium that causes necrotizing wound infec- Revised April 14, 2021 tions and fatal septicemia, which mainly occur in patients with elevated levels of iron in serum or Accepted May 4, 2021 tissue, despite the presence of well-developed bacterial multiple iron-uptake systems (IUSs). These IUSs play important roles in the pathogenesis of V. vulnificus infections and are primar- Corresponding author ily regulated at the transcriptional level by a ferric uptake regulator called Fur responding to iron Sung-Heui Shin availability and their own specific regulators. Recent studies have shown that the IUSs are also Department of Microbiology, College of Medicine, Chosun controlled by other global regulators, including cyclic AMP-receptor protein responding to carbon University, 309 Pilmin-daero, availability and SmcR, a master regulator of the quorum-sensing system responding to bacterial Dong-gu, Gwangju 61452, Korea density. This review presents an update on this sophisticated regulation of IUSs in V. vulnificus. Tel: +82-62-230-6352 Fax: +82-62-608-5314 Keywords: Vibrio vulnificus; Iron; Quorum sensing; Ferric uptake regulator; cAMP-receptor pro- E-mail: [email protected] tein ORCID: https://orcid.org/0000-0003-0688-4161 INTRODUCTION living things is very low. The human body contains 3-5 g of Fe, most of which is intracellular.
  • Photobiology of Bacteria

    Photobiology of Bacteria

    UvA-DARE (Digital Academic Repository) Photobiology of bacteria Hellingwerf, K.J.; Crielaard, W.; Hoff, W.D.; Matthijs, H.C.P.; Mur, L.R.; van Rotterdam, B.J. DOI 10.1007/BF00872217 Publication date 1994 Published in Antonie van Leeuwenhoek Link to publication Citation for published version (APA): Hellingwerf, K. J., Crielaard, W., Hoff, W. D., Matthijs, H. C. P., Mur, L. R., & van Rotterdam, B. J. (1994). Photobiology of bacteria. Antonie van Leeuwenhoek, 65, 331-347. https://doi.org/10.1007/BF00872217 General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl) Download date:02 Oct 2021 Antonie van Leeuwenhoek 65:331-347, 1994. 331 @ 1994 Kluwer Academic Publishers. Printed in the Netherlands. Photobiology of Bacteria K.J. Hellingwerf a, W.
  • The Iron-Dependent Cyanide and Hydrogen Peroxide Co-Toxicity in Escherichia Coli and Its Catastrophic Consequences for the Chromosome

    The Iron-Dependent Cyanide and Hydrogen Peroxide Co-Toxicity in Escherichia Coli and Its Catastrophic Consequences for the Chromosome

    THE IRON-DEPENDENT CYANIDE AND HYDROGEN PEROXIDE CO-TOXICITY IN ESCHERICHIA COLI AND ITS CATASTROPHIC CONSEQUENCES FOR THE CHROMOSOME BY TULIP MAHASETH DISSERTATION Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Microbiology in the Graduate College of the University of Illinois at Urbana-Champaign, 2015 Urbana, Illinois Doctoral Committee: Professor Andrei Kuzminov, Chair and Director of Research Professor John E. Cronan Professor Jeffrey F. Gardner Associate Professor Carin K. Vanderpool ABSTRACT 2+ Hydrogen peroxide (H2O2) can oxidize cytoplasmic ferrous ions (Fe ) to produce highly reactive hydroxyl radicals (•OH) via Fenton’s reaction that can damage various biomolecules causing oxidative stress. Even though at concentrations higher than 20 mM H2O2 by itself can efficiently kill micro-organisms, it is metabolically impossible for eukaryotic cells to generate H2O2, an uncharged molecule, in such large quantities inside the cell. We propose that potentiation of physiologically relevant amounts of H2O2 by various small molecules serves as a more feasible and safe mechanism to combat invading microbes. NO potentiation of H2O2 toxicity is a known bactericidal weapon employed by macrophages. In fact, in human neutrophils activated by bacterial infection, the myeloperoxidase enzyme catalyzes the formation of hydrogen cyanide (HCN) from serum thiocyanate (SCN-). In the past, researchers have reported that a combination of low millimolar doses of H2O2 and cyanide (CN), which are individually bacteriostatic, caused rapid synergistic killing in Escherichia coli. Our aim is to understand the immune cells antimicrobial responses by investigating the mechanism of CN potentiation of H2O2 toxicity and its chromosomal consequences. We have found that the ability of CN to recruit iron from intracellular depots such as ferritin contributes to its potentiation of H2O2 toxicity, whereas the major stationary phase intracellular iron depot protein, Dps, can sequester this iron, thereby quelling Fenton's reaction.
  • Genomic Comparison of Two O111:H− Enterohemorrhagic Escherichia

    Genomic Comparison of Two O111:H− Enterohemorrhagic Escherichia

    crossmark Genomic Comparison of Two O111:H؊ Enterohemorrhagic Escherichia coli Isolates from a Historic Hemolytic-Uremic Syndrome Outbreak in Australia Lauren J. McAllister,a Stephen J. Bent,b Nicola K. Petty,c* Elizabeth Skippington,c Scott A. Beatson,c James C. Paton,a Adrienne W. Patona Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, SA, Australiaa; School of Biological Sciences, University of Adelaide, Adelaide, SA, Australiab; Australian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD, Australiac Downloaded from Enterohemorrhagic Escherichia coli (EHEC) is an important cause of diarrhea and hemolytic-uremic syndrome (HUS) world- wide. Australia’s worst outbreak of HUS occurred in Adelaide in 1995 and was one of the first major HUS outbreaks attributed to a non-O157 Shiga-toxigenic E. coli (STEC) strain. Molecular analyses conducted at the time suggested that the outbreak was caused by an O111:H؊ clone, with strains from later in the outbreak harboring an extra copy of the genes encoding the potent Shiga toxin 2 (Stx2). Two decades later, we have used next-generation sequencing to compare two isolates from early and late in this important outbreak. We analyzed genetic content, single-nucleotide polymorphisms (SNPs), and prophage insertion sites; for the latter, we demonstrate how paired-end sequence data can be leveraged to identify such insertion sites. The two strains are genetically identical except for six SNP differences and the presence of not one but two additional Stx2-converting prophages in http://iai.asm.org/ the later isolate. Isolates from later in the outbreak were associated with higher levels of morbidity, suggesting that the presence of the additional Stx2-converting prophages is significant in terms of the virulence of this clone.
  • Aerobactin Utilization by Neisseria Gonorrhoeae and Cloning of a Genomic DNA Fragment That Complements Escherichia Coli Fhub Mutations SUSAN E

    Aerobactin Utilization by Neisseria Gonorrhoeae and Cloning of a Genomic DNA Fragment That Complements Escherichia Coli Fhub Mutations SUSAN E

    JOURNAL OF BACTERIOLOGY, Aug. 1987, p. 3414-3421 Vol. 169, No. 8 0021-9193/87/083414-08$02.00/0 Copyright © 1987, American Society for Microbiology Aerobactin Utilization by Neisseria gonorrhoeae and Cloning of a Genomic DNA Fragment That Complements Escherichia coli fhuB Mutations SUSAN E. H. WESTt* AND P. FREDERICK SPARLING Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514 Received 17 February 1987/Accepted 28 April 1987 Aerobactin, a dihydroxamate siderophore produced by many strains of enteric bacteria, stimulated the growth of Neisseria gonorrhoeae FA19 and F62 in iron-limiting medium. However, gonococci did not produce detectable amounts of aerobactin in the Escherichia coli LG1522 aerobactin bioassay. We probed gonococcal genomic DNA with the cloned E. coli aerobactin biosynthesis (iucABCD), aerobactin receptor (iutA), and hydroxamate utilization (flwCDB) genes. Hybridization was detected with IhuB sequences but not with the other genes under conditions which will detect 70% or greater homology. Similar results were obtained with 21 additional strains of gonococci by colony filter hybridization. A library of DNA from N. gonorrhoeae FA19 was constructed in the ph id vector XSE4, and a clone was isolated that complemented theihuB mutation in derivatives ofE. coli BU736 and BN3307. These results suggest thatfJhuB is a conserved gene and may play a fundamental role in iron acquisition by N. gonorrhoeae. Most microbial iron acquisition systems consist of two product is apparently a very hydrophobic membrane protein components: a siderophore which is a soluble low-molecu- of 70,337 Mr as calculated from the nucleotide sequence (23); lar-weight iron-chelating compound and a specific cell sur- its exact location in the membranes of E.
  • In Vivo Hyperspectral Confocal Fluorescence Imaging to Determine Pigment Localization and Distribution in Cyanobacterial Cells

    In Vivo Hyperspectral Confocal Fluorescence Imaging to Determine Pigment Localization and Distribution in Cyanobacterial Cells

    In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells Wim F. J. Vermaas*†, Jerilyn A. Timlin‡, Howland D. T. Jones‡, Michael B. Sinclair‡, Linda T. Nieman‡§, Sawsan W. Hamad*, David K. Melgaard‡, and David M. Haaland‡ *School of Life Sciences and Center for Bioenergy and Photosynthesis, Arizona State University, Box 874501, Tempe, AZ 85287-4501; and ‡Sandia National Laboratories, MS0895, Albuquerque, NM 87185 Edited by Elisabeth Gantt, University of Maryland, College Park, MD, and approved January 25, 2008 (received for review August 27, 2007) Hyperspectral confocal fluorescence imaging provides the oppor- cytoplasmic membrane of cyanobacteria, whereas Chl is not (6, tunity to obtain individual fluorescence emission spectra in small 7). Additional light-harvesting capability, primarily for PS II, is (Ϸ0.03-␮m3) volumes. Using multivariate curve resolution, individ- provided by phycobilisomes, which are pigment-binding com- ual fluorescence components can be resolved, and their intensities plexes in the cytoplasm that associate with thylakoids to enable can be calculated. Here we localize, in vivo, photosynthesis-related energy transfer to Chl (8, 9). Phycocyanin (PC), allophycocyanin pigments (chlorophylls, phycobilins, and carotenoids) in wild-type (APC), and allophycocyanin-B (APC-B) are the main phyco- and mutant cells of the cyanobacterium Synechocystis sp. PCC bilisome pigments in Synechocystis sp. PCC 6803 (10). 6803. Cells were excited at 488 nm, exciting primarily phycobilins Chl and phycobilisome pigments fluoresce at room tempera- and carotenoids. Fluorescence from phycocyanin, allophycocyanin, ture with spectral maxima in the 640- to 700-nm range. PC emits allophycocyanin-B/terminal emitter, and chlorophyll a was re- fluorescence with an Ϸ650-nm maximum, APC at 665 nm, and solved.
  • BIOLOGICAL RESEARCH Carlos González Universidad Austral De Chile Sociedad De Biología De Chile Joan Guinovart Universidad De Barcelona Canadá 253, Piso 3O, Dpto

    BIOLOGICAL RESEARCH Carlos González Universidad Austral De Chile Sociedad De Biología De Chile Joan Guinovart Universidad De Barcelona Canadá 253, Piso 3O, Dpto

    %ooz. Biological Research Editorial C. A. JEREZ Colaboración en investigación entre Chile y la Unión Europea BR Sabe Ramón Latorre De La Cruz, Premio Nacional de Ciencias Naturales 2002 Pablo Valenzuela, Premio Nacional de Ciencias Aplicadas y Tecnológicas 2002 Ciencia y Sociedad Volumen Volumen 35 Número 3-4 2002 I Reunión regional de la red SciELO XVI Reunión Anual de la Sociedad de Biología Celular de Chile Biological Research Factor de Impacto 1,154 Articles M. RIOS, A. VENEGAS and H. B. CROXATTO In vivo expression of R-galactosidase by rat oviduct exposed to naked DNA or messenger RNA J. ILLANES, A. DABANCENS, O. ACUÑA, M. FUENZALIDA, A. GUERRERO, C. LOPEZ and D. LEMUS Effects of betamethasone, sulindac and quinacrine drugs on the inflammatory neoangiogenesis response induced by polyurethane sponge implanted in mouse M. GÓTTE and A. STADTBÁUMER Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae C. R. SOTO, J. ARROYO, J. ALCAYAGA Effects of bicarbonate buffer on acetylcholine-, adenosine 5'triphosphate- and cyanide-induced responses in the cat petrosal ganglion in vitro M. GALINDO, M. J. GONZALEZ and N.L GALANTI Echinococcus granulosus protoscolex formation in natural Infections Biological Editor-in-Chief Research__________ Manuel Krauskopf is the continuation since 1992 of Universidad Andrés Bello ARCHIVOS DE BIOLOGIA Y Santiago, Chile MEDICINA EXPERIMENTALES founded in 1964 Associate Editor Founding Editor Jorge Mardones Jorge Garrido Past Editors P. Universidad Católica de Chile Tito Ureta, Patricio Zapata Santiago,
  • Fur Is the Master Regulator of the Extraintestinal Pathogenic

    Fur Is the Master Regulator of the Extraintestinal Pathogenic

    Downloaded from RESEARCH ARTICLE crossmark Fur Is the Master Regulator of the Extraintestinal Pathogenic mbio.asm.org Escherichia coli Response to Serum on August 3, 2015 - Published by Sagi Huja,a Yaara Oren,a Dvora Biran,a Susann Meyer,b Ulrich Dobrindt,c Joerg Bernhard,b Doerte Becher,b Michael Hecker,b Rotem Sorek,d Eliora Z. Rona,e Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israela; Institute for Microbiology, Ernst-Moritz-Arndt-Universität, Greifswald, Germanyb; Institute of Hygiene, Westfälische Wilhelms-Universität, Münster, Germanyc; Department of Molecular Genetics Weizmann Institute of Science, Rehovot, Israeld; MIGAL, Galilee Research Center, Kiriat Shmona, Israele ABSTRACT Drug-resistant extraintestinal pathogenic Escherichia coli (ExPEC) strains are the major cause of colisepticemia (coli- bacillosis), a condition that has become an increasing public health problem in recent years. ExPEC strains are characterized by high resistance to serum, which is otherwise highly toxic to most bacteria. To understand how these bacteria survive and grow in serum, we performed system-wide analyses of their response to serum, making a clear distinction between the responses to nu- tritional immunity and innate immunity. Thus, mild heat inactivation of serum destroys the immune complement and abolishes mbio.asm.org the bactericidal effect of serum (inactive serum), making it possible to examine nutritional immunity. We used a combination of deep RNA sequencing and proteomics in order to characterize ExPEC genes whose expression is affected by the nutritional stress of serum and by the immune complement. The major change in gene expression induced by serum—active and inactive—in- volved metabolic genes.
  • Molecular Epidemiology of Extended-Spectrum Beta-Lactamase

    Molecular Epidemiology of Extended-Spectrum Beta-Lactamase

    Mnif et al. BMC Microbiology 2013, 13:147 http://www.biomedcentral.com/1471-2180/13/147 RESEARCH ARTICLE Open Access Molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli in Tunisia and characterization of their virulence factors and plasmid addiction systems Basma Mnif1,3,4*, Hela Harhour1,4, Jihène Jdidi2, Faouzia Mahjoubi1,4, Nathalie Genel3, Guillaume Arlet3 and Adnene Hammami1,4 Abstract Background: Extended-spectrum β-lactamases (ESBLs), particularly CTX-M- type ESBLs, are among the most important resistance determinants spreading worldwide in Enterobacteriaceae. The aim of this study was to characterize a collection of 163 ESBL-producing Escherichia coli collected in Tunisia, their ESBL-encoding plasmids and plasmid associated addiction systems. Results: The collection comprised 163 ESBL producers collected from two university hospitals of Sfax between 1989 and 2009. 118 isolates harbored blaCTX-M gene (101 blaCTX-M-15 gene and 17 blaCTX-M-14 gene). 49 isolates carried blaSHV-12 gene, 9 blaSHV-2a gene and only 3 blaTEM-26 gene. 16 isolates produced both CTX-M and SHV-12. The 101 CTX-M-15-producing isolates were significantly associated to phylogroup B2 and exhibiting a high number of virulence factors. 24 (23.7%) of the group B2 isolates belonged to clonal complex ST131. Pulsed-field gel electrophoresis (PFGE) typing revealed a genetic diversity of the isolates. 144 ESBL determinants were transferable mostly by conjugation. The majority of plasmid carrying blaCTX-M-15 genes (72/88) were assigned to various single replicon or multireplicon IncF types and had significantly a higher frequency of addiction systems, notably the VagCD module.