Journal of Bacteriology

JOURNAL OF BACTERIOLOGY

Volume 169 June 1987 No. 6

STRUCTURE AND FUNCTION Assembly of a Chemically Synthesized Peptide of Escherichia coli Type 1 Fimbriae into Fimbria-Like Antigenic Structures. Soman N. Abraham and Edwin H. Beachey ...... 2460-2465 Structure of the Staphylococcus aureus Cell Wall Determined by the Freeze- Substitution Method. Akiko Umeda, Yuji Ueki, and Kazunobu Amako ... 2482-2487 Labeling of Binding Sites for 02-Microglobulin (02m) on Nonfibrillar Surface Structures of Mutans Streptococci by Immunogold and I21m-Gold Electron Microscopy. Dan Ericson, Richard P. Ellen, and Ilze Buivids ...... 2507-2515 Bicarbonate and Potassium Regulation of the Shape of Streptococcus mutans NCTC 10449S. Lin Tao, Jason M. Tanzer, and T. J. MacAlister...... 2543-2547 Periodic Synthesis of Phospholipids during the Caulobacter crescentus Cell Cycle. Edward A. O'Neill and Robert A. Bender...... 2618-2623 Association of Thioredeoxin with the Inner Membrane and Adhesion Sites in Escherichia coli. M. E. Bayer, M. H. Bayer, C. A. Lunn, and V. Pigiet 2659-2666 Cell Wall and Lipid Composition of Isosphaera pallida, a Budding Eubacterium from Hot Springs. S. J. Giovannoni, Walter Godchaux III, E. Schabtach, and R. W. Castenholz...... 2702-2707 Charge Distribution on the S Layer of Bacillus stearothermophilus NRS 1536/3c and Importance of Charged Groups for Morphogenesis and Function. Margit Saira and Uwe B. Sleytr ...... 2804-2809 PLANT MICROBIOLOGY Rhizobium meliloti ntrA (rpoN) Gene Is Required for Diverse Metabolic Functions. Clive W. Ronson, B. Tracy Nixon, Lisa M. Albright, and Frederick M. Ausubel...... 2424-2431 Bradyrhizobium japonicum Mutants Defective in Nitrogen Fixation and Molybde- num Metabolism. Robert J. Maier, Lennox Graham, Robert G. Keefe, Todd Pihl, and Eric Smith...... 2548-2554 Variability in Molybdenum Uptake Activity in Bradyrhizobium japonicum Strains. Lennox Graham and Robert J. Maier ...... 2555-2560 A Locus Encoding Host Range Is Linked to the Common Nodulation Genes of Bradyrhizobium japonicum. Anthony J. Nieuwkoop, Zsofia Banfalvi, Nirupama Deshmane, David Gerhold, Maria G. Schell, Karl M. Sirotkin, and Gary Stacey...... 2631-2638 Inhibition of Hydrogenase Synthesis by DNA Gyrase Inhibitors in Bradyrhizobium japonicum. Patricia D. Novak and Robert J. Maier ...... 2708-2712 Flagella of a Plant-Growth-Stimulating Pseudomonasfluorescens Strain Are Re- quired for Colonization of Potato Roots. Letty A. De Weger, Cornelia I. M. van der Vlugt, Andre H. M. Wijfjes, Peter A. H. M. Bakker, Bob Schippers, and Ben Lugtenberg ...... 2769-2773 Nitrogen-Fixing Nodules Induced by Agrobacterium tumefaciens Harboring Rhizo- bium phaseoli Plasmids. Esperanza Martinez, Rafael Palacios, and Federico Sainchez...... 2828-2834 Continued on following page Continuedfrom preceding page GENETICS AND MOLECULAR BIOLOGY Gene Expression in Zymomonas mobilis: Promoter Structure and Identification of Membrane Anchor Sequences Forming Functional LacZ' Fusion Proteins. T. Conway, Y. A. Osman, and L. 0. Ingram ...... 2327-2335 Mutational Alterations Affecting the Export Competence of a Truncated but Fully Functional Maltose-Binding Protein Signal Peptide. John D. Fikes, Vytas

A. Bankaitis, J. Patrick Ryan, and Philip J. Bassford, Jr ...... 2345-2351 Export of Unprocessed Precursor Maltose-Binding Protein to the Periplasm of Escherichia coli Cells. John D. Fikes and Philip J. Bassford, Jr...... 2352-2359 Spectinomycin Resistance and Associated DNA Amplification in Streptomyces ach- romogenes subsp. rubradiris. Ulfert Hornemann, Christopher J. Otto, Guy G. Hoffman, and Anne C. Bertinuson ...... 2360-2366 Photoreactivation in phr Mutants of Escherichia coli K-12. Intisar Husain and Aziz Sancar ...... 2367-2372 Sequence Analysis of the 17-Kilodalton-Antigen Gene from Rickettsia rickettsii. Burt E. Anderson, Russell L. Regnery, George M. Carlone, Theodore

Tzianabos, Joseph E. McDade, Zhang Yuan Fu, and William J. Bellini.... 2385-2390 The phs Gene and Hydrogen Sulfide Production by Salmonella typhimurium. Marta A. Clark and Ericka L. Barrett...... 2391-2397 Regulation of Expression of Pectate Lyase Genes pelA, pelD, and pelE in Erwinia chrysanthemi. Sylvie Reverchon and Janine Robert-Baudouy ...... 2417-2423 Selection of Mutations That Alter the Osmotic Control of Transcription of the Sal- monella typhimurium proU Operon. Janice Druger-Liotta, Virginia Joan Prange, David G. Overdier, and Laszlo N. Csonka ...... 2449-2459 Creation of a Test Plasmid for Detecting G * C-to-T * A Transversions by Chang- ing Serine to Arginine in the Active Site of ,-Lactamase. Patricia L. Foster, Gloria Dalbadie-McFarland, Elaine F. Davis, Steve C. Schultz, and John H. Richards ...... 2476-2481 Regulatory Mutants of the aroF-tyrA Operon of Escherichia coli K-12. Christopher S. Cobbett and Margaret L. Delbridge ...... 2500-2506 Characterization of the sppA Gene Coding for Protease IV, a Signal Peptide Pepti- dase of Escherichia coli. Tohru Suzuki, Akira Itoh, Shigeyuki Ichihara, and Shoji Mizushima ...... 2523-2528 Positive and Negative Regulation of the bgl Operon in Escherichia coli. Subramony Mahadevan, Ann E. Reynolds, and Andrew Wright ...... 2570-2578 ,-Glucoside (bgl) Operon of Escherichia coli K-12: Nucleotide Sequence, Genetic Organization, and Possible Evolutionary Relationship to Regulatory Compo- nents of Two Bacillus subtilis Genes. Karin Schnetz, Christian Toloczyki, and Bodo Rak ...... 2579-2590 Cloning and Sequencing of the Alcohol Dehydrogenase II Gene from Zymomonas

mobilis. T. Conway, G. W. Sewell, Y. A. Osman, and L. 0. Ingram ..... 2591-2597 Genetic Characterization of a Highly Efficient Alternate Pathway of Serine Bio- synthesis in Escherichia coli. Paula D. Ravnikar and Ronald L. Somerville 2611-2617 Operator Sequences of the Aerobactin Operon of Plasmid ColV-K30 Binding the Ferric Uptake Regulation (fur) Repressor. Victor de Lorenzo, Sechan Wee, Marta Herrero, and J. B. Neilands ...... 2624-2630 Identification, Mapping, and Cloning of the Gene Encoding Cyanase in Escherich- ia coli K-12. Young-Chul Sung, Dawn Parsell, Paul M. Anderson, and James A. Fuchs ...... 2639-2642 Isolation of Haemophilus influenzae Genes That Suppress Escherichia coli polA Mutations. Gene L. Williams, Brent Seaton, and David McCarthy ...... 2643-2649 Continued on following page Continuedfrom preceding page

Nucleotide Sequence of a Gene Cluster Involved in Entry of E Colicins and Single- Stranded DNA of Infecting Filamentous Bacteriophages into Escherichia coli. Tai-Ping Sun and Robert E. Webster ...... 2667-2674 Isolation and Characterization of Light-Regulated Phycobilisome Linker Polypep- tide Genes and Their Transcription as a Polycistronic mRNA. Terri L. Lomax, Pamela B. Conley, James Schilling, and Arthur R. Grossman..... 2675-2684 Expression and Regulation of a Vibrio alginolyticus Sucrose Utilization System Cloned in Escherichia coli. Renate R. Scholle, Vernon E. Coyne, Romilla Maharaj, Frank T. Robb, and David R. Woods ...... 2685-2690 Cloning and Characterization of Elastase Genes from Pseudomonas aeruginosa. Peter A. Schad, Robert A. Bever, Thalia I. Nicas, Francois Leduc, Larry F. Hanne, and Barbara H. Iglewski...... 2691-2696 Posttranscriptional Regulation of Ribosomal Protein S20 and Stability of the S20 mRNA Species. George A. Mackie ...... 2697-2701 Suppressible Base Substitution Mutations Induced by Angelicin (Isopsoralen) in the Escherichia coli lacI Gene: Implications for the Mechanism of SOS Mutagenesis. Stephan S. Miller and Eric Eisenstadt ...... 2724-2729 Genetic Transformation in the Methanogen Methanococcus voltae PS. G. Bertani and L. Baresi ...... 2730-2738 Molecular Cloning and Characterization of the recA Gene from the Cyanobacte- rium Synechococcus sp. Strain PCC 7002. Randy C. Murphy, Donald A. Bryant, Ronald D. Porter, and Nicole Tandeau de Marsac ...... 2739-2747 DNA Replication by a DNA-Membrane Complex Extracted from Bacillus subtilis: Site of Initiation In Vitro and Initiation Potential of Subcomplexes. John Laffan and William Firshein ...... 2819-2827 Promoter of the Pertussis Toxin Operon and Production of Pertussis Toxin. Alfredo Nicosia and Rino Rappuoli ...... 2843-2846 Bordetella parapertussis and Bordetella bronchiseptica Contain Transcriptionally Silent Pertussis Toxin Genes. Beatrice Arico and Rino Rappuoli ...... 2847-2853 Genetic and Physical Analyses of a Cluster of Genes Essential for Xanthan Gum Biosynthesis in Xanthomonas campestris. Nancy E. Harding, Joseph M. Cleary, Debra K. Cabafias, Ira G. Rosen, and Kenneth S. Kang...... 2854-2861 Nucleotide Sequence of the Gene for the Hole-Forming Toxin Aerolysin of Aero- monas hydrophila. S. Peter Howard, William J. Garland, Margaret J. Green, and J. Thomas Buckley...... 2869-2871 Gene Location Affects Expression Level in Salmonella typhimurium. Molly B. Schmid and John R. Roth ...... 2872-2875 In Vitro Transcription of the Nitrogen Fixation Regulatory Operon niJLA of Kleb- siella pneumoniae. P.-K. Wong, D. Popham, J. Keener, and S. Kustu .... 2876-2880 An Inducible Pathway Is Required for Mutagenesis in Salmonella typhimurium LT2. Cristian Orrego and Eric Eisenstadt ...... 2885-2888 Common Evolutionary Origin of the ilvGMEDA Attenuation Locus and tRNAleu in Escherichia coli. Rufus M. Williamson and Julius H. Jackson ...... 2893-2895 Acylation of Glycerol 3-Phosphate Is the Sole Pathway of De Novo Phospholipid Synthesis in Escherichia coli. Tapas K. Ray and John E. Cronan, Jr...... 2896-2898 Evidence that Colicin X Is Microcin B17. Jose L. San Millan, Roberto Kolter, and Felipe Moreno ...... 2899-2901 Molecular Cloning and Biological Characterization of the recA Gene from Pseudo- monas syringae. Margaret J. Hickman, Cindy S. Orser, David K. Willis, Steven E. Lindow, and Nickolas J. Panopoulos...... 2906-2910 Continued on following page Continuedfrom preceding page

PLASMIDS AND TRANSPOSONS Molecular Characterization of the virC Genes of the Ti Plasmid. T. J. Close, R. C. Tait, H. C. Rempel, T. Hirooka, L. Kim, and C. I. Kado ...... 2336-2344 Identification and Expression of a Copy Number Control Gene in the IncFIII He- molytic Plasmid pSU316. Isabel Andres, Jose C. Rodriguez, Javier Barbadillo, and Jose M. Ortiz...... 2405-2409 Four Plasmid Genes Are Required for Colicin V Synthesis, Export, and Immunity. Lynne Gilson, Hare Khrisna Mahanty, and Roberto Kolter 2466-2470 Two Conjugation Systems Associated with Streptococcus faecalis Plasmid pCF10: Identification of a Conjugative Transposon That Transfers between S. faecalis and Bacillus subtilis. Peter J. Christie, Ruth Z. Korman, Stanley A. Zahler, Jean C. Adsit, and Gary M. Dunny...... 2529-2536 Molecular Cloning of Invasion Plasmid Antigen (ipa) Genes from Shigella flexneri: Analysis of ipa Gene Products and Genetic Mapping. Jerry M. Buysse, Charles K. Stover, Edwin V. Oaks, Malabi Venkatesan, and Dennis J. Kopecko ...... 2561-2569 A Site-Specific Recombination Function in Staphylococcus aureus Plasmids. Maria L. Gennaro, John Kornblum, and Richard P. Novick...... 2601-2610 Stability and Replication Control of Escherichia coli Minichromosomes. Anders L0bner-Olesen, Tove Atlung, and Knud V. Rasmussen...... 2835-2842 Characterization of Transfer Regions within the HII Incompatibility Group Plas- mid pHHl508a. William Yan and Diane E. Taylor ...... 2866-2868 EUCARYOTIC CELLS Cloning and Characterization of Saccharomyces cerevisiae Genes That Confer L- Methionine Sulfoximine and Tabtoxin Resistance. Elzbieta T. Marek and Robert C. Dickson...... 2440-2448 Ureidosuccinate Is Transported by the Allantoate Transport System in Saccharo- myces cerevisiae. Vanessa Turoscy and Terrance G. Cooper ...... 2598-2600 Differential DNA Methylation during the Vegetative Life Cycle of Neurospora crassa. Peter J. Russell, Karin D. Rodland, Elliot M. Rachlin, and James A. McCloskey ...... 2902-2905 MEMBRANES Effects of Antibiotics and Other Inhibitors on ATP-Dependent Protein Transloca- tion into Membrane Vesicles. Lingling Chen and Phang C. Tai ...... 2373-2379 Modulation of Cell Wall Synthesis by DNA Replication in Escherichia coli during Initiation of Cell Growth. Francisco Garcia del Portillo, Antonio G. Pisabarro, Enrique J. de la Rosa, and Miguel A. de Pedro ...... 2410-2416 Neutral Amino Acid Transport by Membrane Vesicles of Streptococcus cremoris Is Subject to Regulation by Internal pH. Arnold J. M. Driessen, Jan Kodde, Steven de Jong, and Wil N. Konings ...... 2748-2754 Kinetic Properties of a Phosphate-Bond-Driven Glutamate-Glutamine Transport System in Streptococcus lactis and Streptococcus cremoris. Bert Poolman, Eddy J. Smid, and Wil N. Konings ...... 2755-2761 Characterization of Mutant Histidine-Containing Proteins of the Phosphoenolpyr- uvate:Sugar Phosphotransferase System of Escherichia coli and Salmonella typhimurium. E. Bruce Waygood, Bernd Reiche, Wolfgang Hengstenberg, and Jeremy S. Lee ...... 2810-2818 Putrescine and Cadaverine Are Constituents of Peptidoglycan in Veillonella al- calescens and Veillonella parvula. Yoshiyuki Kamio and Kazuo Nakamura 2881-2884 Continued on following page Continuedfrom preceding page PHYSIOLOGY AND METABOLISM 2-Methylthio-1,4-Naphthoquinone, a Unique Sulfur-Containing Quinone from a Thermophilic Hydrogen-Oxidizing Bacterium, Hydrogenobacter thermophilus. Masharu Ishii, Toshiyuki Kawasumi, Yasuo Igarashi, Tohru Kodama, and Yasuji Minoda ...... 2380-2384 Initial Catabolism of Aromatic Biogenic Amines by Pseudomonas aeraginosa PAO: Pathway Description, Mapping of Mutations, and Cloning of Essential Genes. Stephen M. Cuskey, Vincent Peccoraro, and Ronald H. Olsen ... 2398-2404 Streptococcusfaecium Mutants That Are Temperature Sensitive for Cell Growth and Show Alterations in Penicillin-Binding Proteins. Pietro Canepari, Maria Del Mar Lle6, Roberta Fontana, and Giuseppe Satta ...... 2432-2439 Regulation of Expression of Glutamine Synthetase in a Symbiotic Nostoc Strain Associated with Anthoceros punctatus. Cecillia M. Joseph and John C. Meeks . 2471-2475 Metabolism of L-Glyceraldehyde 3-Phosphate in Escherichia coli. Metaramba K. G. S. Kalyananda, Robert Engel, and Burton E. Tropp...... 2488-2493 Role of Oxygen Radicals in the Phototoxicity of Tetracyclines toward Escherichia coli B. Joseph P. Martin, Jr., Kenneth Colina, and Naomi Logsdon...... 2516-2522 Alteration of the Fe Protein of Nitrogenase by Oxygen in the Cyanobacterium Anabaena sp. Strain CA. Russell L. Smith, Chase Van Baalen, and F. Robert Tabita ...... 2537-2542 Mode of Initiation of Constitutive Stable DNA Replication in RNase H-Defective Mutants of Escherichia coli K-12. Kaspar von Meyenburg, Erik Boye, Kirsten Skarstad, Luud Koppes, and Tokio Kogoma ...... 2650-2658 Tetrahydrothiophene 1-Oxide as an Electron Acceptor for Escherichia coli. R. Meganathan and J. Schrementi ...... 2862-2865 Recruitment of Naphthalene Dissimilatory Enzymes for the Oxidation of 1,4-Di- chloronaphthalene to 3,6-Dichlorosalicylate, a Precursor for the Herbicide Dicamba. Don R. Durham and David B. Stewart ...... 2889-2892 ENZYMOLOGY Alkylation of Acetohydroxyacid Synthase I from Escherichia coli K-12 by 3-Bro- mopyruvate: Evidence for a Single Active Site Catalyzing Acetolactate and Acetohydroxybutyrate Synthesis. Philip M. Silverman and Lillian Eoyang 2494-2499 An Aminoacyl-tRNA Synthetase Complex in Escherichia coli. Charles L. Harris 2718-2723 Novel Alkaline- and Heat-Stable Serine Proteases from Alkalophilic Bacillus sp. Strain GX6638. Don R. Durham, David B. Stewart, and E. J. Stellwag 2762-2768 Inducible Thermoalkalophilic Polygalacturonate Lyase from Thermomonospora fusca. Fred J. Stutzenberger ...... 2774-2780 POPULATION GENETICS AND EVOLUTION Functional Genes for Cellobiose Utilization in Natural Isolates of Escherichia coli. Barry G. Hall and Wesley Faunce III...... 2713-2717 Genetic Structure of Neisseria meningitidis Populations in Relation to Serogroup, Serotype, and Outer Membrane Protein Pattern. Dominique A. Caugant, Louis F. Mocca, Carl E. Frasch, L. Oddvar Fr0holm, Wendell D. Zollinger, and Robert K. Selander...... 2781-2792 Clonal Diversity and Host Distribution in Bordetella bronchiseptica. James M. Musser, David A. Bemis, Hitoshi Ishikawa, and Robert K. Selander...... 2793-2803

Continued on following page Continuedfrom preceding page ERRATA Operator-Constitutive Mutations of the Escherichia coli metF Gene. Jamila Belfaiza, Yvonne Guillou, Danielle Margarita, David Perrin, and Isabelle Saint Girons ...... 2911 Structure of the Azotobacter vinelandii Surface Layer. Wade H. Bingle, Patrick W. Whippey, James L. Doran, Robert G. E. Murray, and William J. Page 2911 Lipopolysaccharide Size and Distribution Determine Serum Resistance in Salmo- nella montevideo. Nili Grossman, Mark A. Schmetz, John Foulds, Emilie N. Klima, Victor E. Jimenez-Lucho, Loretta L. Leive, and Keith A. Joiner 2911

Date of Issue: 29 May 1987