FBXO5, ID (FBXO5, EMI1, FBX5, F-box only 5, Early mitotic inhibitor 1)

Catalog number 035565 Supplier United States Biological

This encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box constitute one of the four subunits of the protein ligase complex called SCFs (SKP1--F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. Applications Suitable for use in Western Blot, ELISA Recommended Dilution ELISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Immunogen FBXO5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-221 amino acids from the Central region of human FBXO5. Formulation Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity Purified by Protein A affinity chromatography. Specificity Human Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications E WB Crossreactivity Hu Storage -20°C Reference Lei, S.F., et al. J. Bone Miner. Res. (2010) In press : Ma, H.T., et al. Mol. Cell. Biol. 29(24):6500-6514(2009) Lee, J., et al. Mol. Biol. Cell 20(7):1891-1902(2009) Gutgemann, I., et al. Mod. Pathol. 21(4):445-454(2008) Iwai, H., et al. Cell 130(4):611-623(2007)

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