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Research Day Book of Abstracts 7KH7XDQ\RN/DERUDWRU\LVLQYHVWLJDWLQJWKHLPSDFWVRIPHOLRLGRVLV LQ6RXWKHDVW$VLD%XUNKROGHULDSVHXGRPDOOHLDVRLOEDFWHULXPLVWKH EPI RESEARCH FDXVDWLYHDJHQWRIWKHQHJOHFWHGWURSLFDOGLVHDVHPHOLRLGRVLV7XDQ\RN /DERUDWRU\KDVLPSOHPHQWHGD³2QH+HDOWK´DSSURDFKWRLQYHVWLJDWHWKH RESEARCH DAY EXUGHQVRIPHOLRLGRVLVLQKXPDQVDQGDQLPDOVDVZHOODVWKHRFFXUUHQFH RI%SVHXGRPDOOHLLQVRLODQGZDWHUWKURXJKRXW6RXWKHDVW$VLD BOOK OF ABSTRACTS 7KHLPDJHRQWKHFRYHUGHSLFWVERWKGU\DQGZULQNOHFRORQLHVRI %SVHXGRPDOOHLDPRQJRWKHUEDFWHULDJURZQRQ$VKGRZQ¶VDJDUIURPD VRLOVDPSOHFROOHFWHGIURPDJRDWIDUPLQVRXWKHUQ7KDLODQG Emerging Pathogens Institute )RUPRUHLQIRUPDWLRQSOHDVHYLVLW KWWSZZZYHWPHGXIOHGXDERXWWKHFROOHJH IDFXOW\GLUHFWRU\DSLFKDLWXDQ\RN DA Y BOOK OF ABSTRA CT S 2018 FEBRUARY 2018 EPI RESEARCH DAY 2018 Table of Contents Page 2 Letter from the Director Page 3 Schedule of Events Page 4 Keynote Speakers Page 5-34 Enteric and Foodborne Pathogens Page 35-42 Influenza and Respiratory Viruses Page 43-48 Parasitic and Fungal Diseases Page 49-57 Tuberculosis and Mycobacterial Diseases Page 58-148 Vector-borne Diseases Page 149-180 Other Bacterial Pathogens Page 181-203 Other Viral Pathogens Page 204-231 Other Topic Areas Page 232-244 Index 1 EPI RESEARCH DAY 2018 Letter from the Director Welcome to the eleventh annual EPI Research Day! As you look through the abstracts in this book, and view the correlating posters, you should get a feel for the wide range of emerging pathogen-related research conducted by EPI members and collaborators. We are particularly pleased to welcome investigators from outside of UF, including investigators from the Florida Department of Health and other collaborating universities. This year we have the honor of introducing you to two outstanding investigators visiting us to give speeches during our afternoon session. Peter M. Small, M.D., is the founding director of the Stony Brook University Global Health Institute. His expertise includes tuberculosis (TB) and global health. For more than a decade Dr. Small was responsible for building and running the innovative TB program at the Bill & Melinda Gates Foundation. He has done seminal work on the clinical, epidemiologic, evolutionary, and genetic aspects of tuberculosis. He has deep expertise in translating cutting edge science into drugs, diagnostic methods and vaccines as well as in the business and public health processes to get innovative tools to those in need. He is joined by Dr. Andrew Pekosz, co-director of the Johns Hopkins Center for Excellence in Influenza Research and Surveillance and director of the Center for Emerging Viral Infectious Diseases. Dr. Pekosz has served on a number of National Institute of Health scientific and policy review boards focused on biosafety and biocontainment and is an expert on the topics of influenza, biosafety, emerging infectious diseases and pandemic preparedness. Please visit our website, www.epi.ufl.edu, to join our listservs and to keep up with our news, events and seminars throughout the year. And thanks for coming! J. Glenn Morris, Jr. M.D., M.P.H. & T.M. EPI Director and Professor of Medicine 2 EPI RESEARCH DAY 2018 Schedule of Events 9:00 AM - 10:00 AM Registration, Breakfast, and Poster Setup Reitz Union – Rion Ballroom 10:00 AM - 1:00 PM Poster Session Presenters, please stand by your posters 12:00 PM - 12:45 PM Lunch Reitz Union – Rion Ballroom 12:45 PM - 1:00 PM Keynote Assembly Reitz Union – Rion Ballroom 1:00 PM - 1:10 PM Welcome Dr. Jack M. Payne, Senior VP for Agriculture and Natural Resources Head of the Institute of Food and Agricultural Sciences (IFAS) Introductions Dr. J. Glenn Morris, Director, EPI 1:10 PM - 3:15 PM Keynote Speeches 3:15 PM - 4:00 PM Poster Removal 3 EPI RESEARCH DAY 2018 Keynote Speakers (1:10-2:10) Dr. Peter Small Founding Director and Jim and Robin Hernstein Chair of the Global Health Institute at Stony Brook University “Disrupting TB Care and Control” (2:10-3:10) Dr. Andrew Pekosz Professor of Molecular Microbiology and Immunology and Director of the Center for Emerging Viral Infectious Diseases at the Johns Hopkins Bloomberg School of Public Health “Surveillance for Human Influenza Virus Infections: More Than Just Choosing Vaccine Strains” 4 01. A MECHANISTIC BACTERIAL TRANSPORT MODEL TO INFORM FOOD SAFETY MANAGEMENT OF AGRICULTURAL POND WATER Kathleen Vazquez - Department of Agricultural and Biological Engineering, University of Florida Water Institute, College of Agricultural and Life Sciences, University of Florida; Arie Havelaar - Department of Animal Sciences, Emerging Pathogens Institute, College of Agricultural and Life Sciences, University of Florida; Rafael Muñoz-Carpena - Department of Agricultural and Biological Engineering, College of Agricultural and Life Sciences, University of Florida Irrigation water is considered a major pathway to fresh produce for food-borne illness related pathogens. The Food Safety Modernization Act (FSMA) specifies sampling-based methods using Escherichia coli as an indicator organism and microbial criteria, geometric mean (GM) and statistical threshold value (STV), to regulate agricultural water. These regulations lack preventive measures, important to controlling the processes that introduce bacterial contamination into water sources. To inform FSMA regulations concerning agricultural water, this study develops a simple mechanistic model to predict the microbial quality of agricultural water. This model proved useful to simulate data from a highly variable surface water irrigation pond in West Central Florida. The performance of the model was similar or superior to existing pathogen transport models, with a Nash-Sutcliffe efficiency of 0.455 when incorporating observed value uncertainty. Global sensitivity analysis was used to reveal the most important processes controlling bacterial water quality criteria: aquatic removal rate and transport dynamics of bacteria for GM, and bacterial source and transport dynamics for STV. It was also found that large peak E. coli concentration events were mechanistically driven by rainfall/runoff processes. From these findings, we suggest improved sampling schedules that focus on peak event timing and practices that exclude wildlife and other potential bacterial sources. Vegetative filter strips, when properly designed and maintained, also provide an 5 opportunity to mitigate bacterial transfer into the agricultural waters by reducing runoff flow and promoting pollutant settling. 02. ACTUATION OF CHITOSAN-APTAMER NANOBRUSH BORDERS FOR PATHOGEN SENSING Eric McLamore - Department of Agricultural and Biological Engineering, College of Agricultural and Life Sciences, University of Florida; Carmen Gomes - Department of Mechanical and Aerospace Engineering, Iowa State University; Nick Cavallaro - Department of Agricultural and Biological Engineering, College of Engineering, University of Florida We demonstrate a sensing mechanism for rapid detection of Listeria monocytogenes in food samples using the actuation of chitosan- aptamer nanobrush borders. The bio-inspired soft material and sensing strategy mimic natural symbiotic systems, where low levels of bacteria are selectively captured from complex matrices. To engineer this biomimetic system, we first develop reduced graphene oxide/nanoplatinum (rGO-nPt) electrodes, and characterize the fundamental electrochemical behavior in the presence and absence of chitosan nanobrushes during actuation (pH-stimulated osmotic swelling). We then characterize the electrochemical behavior of the nanobrush when receptors (antibodies or DNA aptamers) are conjugated to the surface. Finally, we test various techniques to determine the most efficient capture strategy based on nanobrush actuation, and then apply the biosensors in a food product. Maximum cell capture occurs when aptamers conjugated to the nanobrush bind cells in the extended conformation (pH < 6), followed by impedance measurement in the collapsed nanobrush conformation (pH > 6). The aptamer-nanobrush hybrid material was more efficient than the antibody-nanobrush material, which was likely due to the relatively high adsorption capacity for aptamers. The biomimetic material was used to develop a rapid test (17 min) for selectively detecting L. monocytogenes at concentrations ranging from 9 to 10^7 CFU-mL-1 with no pre-concentration, and in the presence of other gram-positive cells (Listeria innocua and Staphylococcus aureus). Use of this bio-inspired material is among 6 the most efficient for L. monocytogenes biosensing to date, and does not require sample pretreatment, making nanobrush borders a promising new material for rapid pathogen detection in food. 03. AN ALTERNATIVE RISK RANKING METHOD BASED ON LOG TRANSFORMATION FOR RANKING PRODUCE-HAZARD PAIRS Min Li - Department of Animal Sciences, Institute of Food and Agricultural Sciences, College of Agricultural and Life Sciences, University of Florida; Moez Sanaa - French Agency for Food, Environmental and Occupational Health & Safety; Barbara Kowalcyk - Ohio State University; Kostas Koutsoumanis - Aristotle University of Thessaloníki; Arie Havelaar - Department of Animal Sciences, Emerging Pathogens Institute, College of Agricultural and Life Sciences, University of Florida Risk ranking approaches can help identify and prioritize foods and/or hazards that may pose greatest risks to public health. The use of semiquantitative risk ranking methods are relatively simple and flexible, but could result in substantial loss of information and limited resolution. The study aimed to compare published semiquantitative risk
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