Emery and Offord (2019). Seed Science and Technology, 47, 1, 107-112. https://doi.org/10.15258/sst.2019.47.1.12 Research Note The effect of the endocarp, heat-shock and short-term storage on the germination of Persoonia hirsuta (Proteaceae) seeds Nathan J. Emery* and Catherine A. Offord The Australian PlantBank, the Royal Botanic Gardens and Domain Trust, the Australian Botanic Garden Mount Annan, NSW 2567 Australia * Author for correspondence (E-mail:
[email protected]) (Submitted November 2018; Accepted February 2019; Published online April 2019) Abstract Persoonia are Proteaceous shrubs or small trees that have a woody endocarp as part of the dispersal unit. Seeds with this structure are often difficult to germinate. The endangered Persoonia hirsuta (hairy geebung) is a high- interest plant species for which no previous seed biology research has been conducted. In the present study we investigated the seed germination response to heat and short-term ex situ storage at low and room temperature. The effect of pre- and post-storage endocarp removal on germination was also examined. Germination success was significantly reduced when the endocarp was removed prior to storage due to increased microbial contamination coming from within the seeds. There was no significant effect of heat-shock on germination. Based on these findings, it is recommended that the endocarp be retained when storing or direct-sowing P. hirsuta seeds to enhance the likelihood of producing viable seedlings. Keywords: germination, hairy geebung, microbial contamination, Proteaceae, pyrene, seed biology Experimental and discussion A Persoonia fruit is a drupe which has an outer fleshy mesocarp and leathery pericarp layers, and a hard, woody endocarp within that encapsulates either one or two embryos that are surrounded by a thin, papery testa.