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Human/Mouse/Rat HIF-1 Alpha/HIF1A Antibody

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Human/Mouse/Rat HIF-1 Alpha/HIF1A Antibody Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody Monoclonal Mouse IgG1 Clone # 241809 Catalog Number: MAB1536 DESCRIPTION Species Reactivity Human/Mouse/Rat Specificity Detects human, mouse, and rat HIF-1 alpha/HIF1A. Source Monoclonal Mouse IgG1 Clone # 241809 Purification Protein A or G purified from hybridoma culture supernatant Immunogen E. coli-derived recombinant human HIF-1 alpha/HIF1A Arg575-Asn826 Accession # Q16665.1 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS. APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Western Blot 1 µg/mL See Below Immunohistochemistry 5-25 µg/mL See Below Immunoprecipitation 1-3 µg/500 µg cell MCF‑7 human breast cancer cell line treated with CoCl2, see our available Western blot lysate detection antibodies Simple Western 10 µg/mL See Below Knockout Validated HIF-1 alpha/HIF1A is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HIF-1 alpha/HIF1A knockout HeLa cell line. DATA Western Blot Immunohistochemistry Detection of Human, Mouse, HIF-1 alpha/HIF1A in Human Kidney. and Rat HIF-1 alpha/HIF1A by HIF-1 alpha/HIF1A was detected in Western Blot. Western blot immersion fixed paraffin-embedded sections shows lysates of MCF-7 human of human kidney using Mouse Anti- breast cancer cell line, Balb-3T3 Human/Mouse/Rat HIF-1 alpha/HIF1A mouse embryonic fibroblast cell Monoclonal Antibody (Catalog # MAB1536) line, and PC-12 rat adrenal at 5 µg/mL for 1 hour at room temperature pheochromocytoma cell line followed by incubation with the Anti-Mouse untreated (-) or treated (+) with IgG VisUCyte™ HRP Polymer Antibody 150 μM CoCl2 for 8 hours. (Catalog # VC001). Before incubation with PVDF membrane was probed the primary antibody, tissue was subjected to with 1 µg/mL of Mouse Anti- heat-induced epitope retrieval using Antigen Human/Mouse/Rat HIF-1 Retrieval Reagent-Basic (Catalog # alpha/HIF1A Monoclonal CTS013). Tissue was stained using DAB Antibody, followed by HRP- (brown) and counterstained with hematoxylin conjugated Anti-Mouse IgG (blue). Specific staining was localized to Secondary Antibody (Catalog # cytoplasm and nuclei. View our protocol for HAF007). A specific band was IHC Staining with VisUCyte HRP Polymer detected for HIF-1 alpha/HIF1A Detection Reagents. at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Rev. 10/7/2019 Page 1 of 2 Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody Monoclonal Mouse IgG1 Clone # 241809 Catalog Number: MAB1536 Simple Western Knockout Validated Detection of Human HIF-1 alpha/HIF1A Western Blot Shows Human HIF-1 by Simple WesternTM . Simple Western lane alpha/HIF1A alpha/HIF1A Specificity by view shows lysates of A549 human lung Using Knockout Cell Line. Western blot carcinoma cell line untreated (-) or treated shows lysates of HeLa human cervical (+) with Hypoxia (1% O2), loaded at epithelial carcinoma parental cell line and 0.2 mg/mL. A specific band was detected for HIF-1 alpha/HIF1A knockout HeLa cell line HIF-1 alpha/HIF1A at approximately 116 kDa (KO) untreated (-) or treated (+) with 1 mM (as indicated) using 10 µg/mL of Mouse Anti- DFO overnight. PVDF membrane was probed Human/Mouse/Rat HIF-1 alpha/HIF1A with 1 µg/mL of Mouse Anti- Monoclonal Antibody (Catalog # MAB1536). Human/Mouse/Rat HIF-1 alpha/HIF1A This experiment was conducted under Monoclonal Antibody (Catalog # MAB1536) reducing conditions and using the followed by HRP-conjugated Anti-Mouse IgG 12-230 kDa separation system. Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha/HIF1A at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Knockout Validated HIF-1 alpha/HIF1A Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF-1 alpha/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1 alpha/HIF1A knockout (KO) HeLa Human Cell Line cell line using Mouse Anti- Human/Mouse/Rat HIF-1 alpha/HIF1A alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557- conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (-SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. BACKGROUND The hypoxia-inducible transcription factor 1α (HIF-1α) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1α. Rev. 10/7/2019 Page 2 of 2 .
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