Thrombopoietin, human recombinant, expressed in E. coli

Catalog Number T1568 Storage Temperature –20 °C

Synonyms: c-MPL ligand, Precautions and Disclaimer colony-stimulating factor, MGDF, TPO This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Product Description Safety Data Sheet for information regarding hazards Recombinant Human Thrombopoietin (TPO), the ligand and safe handling practices. for the receptor encoded by the c-Mpl proto-oncogene, is a primary regulatory factor of megakaryocytopoiesis Preparation Instructions and thrombopoiesis in vitro and in vivo.1-4 Thrombo- Reconstitute the contents of the vial using 10 mM poietin is produced from a DNA sequence encoding the sodium citrate, pH 3.0, to a concentration of less than mature human thrombopoietin .4 Human and 0.5 mg/mL. This solution can then be diluted into other murine TPO exhibit cross-species reactivity. aqueous buffers. Reconstituted product may be stored at 2–8 °C for up to one week. For extended storage, Thrombopoietin (TPO) is a highly conserved freeze in working aliquots. . It is a lineage specific produced in the , , and skeletal muscle. TPO Storage/Stability stimulates the proliferation and maturation of Prior to reconstitution, store at –20 °C. Reconstituted and promotes increased circulating product may be stored at 2–8 °C for up to one week. levels of in vivo. TPO signals through the For prolonged storage, freeze in working aliquots. Avoid c-mpl receptor and acts as an important regulator of repeated freezing and thawing. Do not store in frostfree circulating platelets. TPO binds to its receptor, c-Mpl freezer. proto-oncogene product, at two distinct sites, initiating 5 receptor dimerization and activation. Analysis of c-Mpl References proto-oncogene mRNA indicates the existence of a 1. Lok, S., and Foster, D.C., The structure, biology, novel truncated and potentially soluble form of TPO and potential therapeutic applications of receptor. TPO receptors are found on megakaryocytes, recombinant thrombopoietin. Stem Cells, 12, 586- their precursors, and platelets. 598 (1994). 2. Kato, T., et al., Native thrombopoietin: structure and This recombinant, human TPO product is a fully function. Stem Cells, 16 (suppl. 2), 11-19 (1998). biologically active 174 polypeptide 3. Kuter, D., et al., eds., Thrombopoiesis and (18.6 kDa), which contains the -like Thrombopoietins, Humana Press (Totowa, N.J.: domain of the full length TPO protein. It is supplied as 1997). ~5 mg of protein lyophilized from a 0.1 % TFA solution 4. Foster, D., et al., Human thrombopoietin: containing 250 mg bovine serum albumin. structure, cDNA sequence, expression, and chromosomal localization. Proc. Natl. Acad. Sci., The biological activity of this recombinant product is 91, 13023-13027 (1994). measured in a cell proliferation assay using human 5. Hou, J., and Zhan, H., Expression of active MO7e cells.6 thrombopoietin and identification of its key residues responsible for receptor binding. , 10, 319- Purity: ³98% (SDS -PAGE and HPLC) 330 (1998). 6. Avanzi, G., et al., Selective growth response to IL-3 Endotoxin: <0.1 ng/mg (1 EU/ mg) of human leukemic cell line with megakaryoblastic features. Br. J. Haematol., 69, 359-366 (1988).

BKR,KAA,MAM 06/10-1

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