Catalog # Aliquot Size

U213-911-05 3 x 5 nmol U213-911-20 3 x 20 nmol U213-911-50 3 x 50 nmol

UBE2D1 (UBCH5A) siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # U213-911

Lot # Z2109-18

Specificity Formulation UBE2D1 (UBCH5A) siRNAs are designed to specifically The siRNAs are supplied as a lyophilized powder and knock-down human UBE2D1 (UBCH5A) expression. shipped at room temperature.

Product Description Reconstitution Protocol UBE2D1 (UBCH5A) siRNA is a pool of three individual Briefly centrifuge the tubes (maximum RCF 4,000g) to synthetic siRNA duplexes designed to knock-down collect lyophilized siRNA at the bottom of the tube. human UBE2D1 (UBCH5A) mRNA expression. Each siRNA is Resuspend the siRNA in 50 µl of DEPC-treated water 19-25 bases in length. The accession number is (supplied by researcher), which results in a 1x stock NM_003338. solution (10 µM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot Gene Aliases 1x stock solutions for storage. E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the UBE2A U210-30H lyophilized and resuspended siRNAs are stored at or UBE2B Protein U211-30H below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2C Protein U212-30H µM can undergo up to 50 freeze-thaw cycles without UBE2D1 (UBCH5A) U213-30H significant degradation. For long-term storage, it is Protein recommended that the siRNA is stored at -70oC. For most UBE2D2 (UBC4) Protein U214-30H favorable performance, avoid repeated handling and UBE2D3 (UBCH5C) U215-30H multiple freeze/thaw cycles. Protein

Scientific Background UBE2D1 (UBCH5A) or -conjugating enzyme E2D1 is a member of the E2 ubiquitin-conjugating enzyme UBE2D1 (UBCH5A) siRNA Set I family which is closely related to a stimulator of iron siRNA duplexes targeted against three exon regions transport (SFT). UBE2D1 is significantly upregulated in livers of iron-overloaded patients with hereditary Catalog # U213-911 hemochromatosis (1). UBE2D1 also functions in the Lot # Z2109-18 ubiquitination of the tumor-suppressor protein p53 and Packaging Specifications 2.5 nmol/tube for 3 x 5nmol the hypoxia-inducible transcription factor HIF-1α by Format Lyophilized powder interacting with the E1 ubiquitin-activating enzyme and Stability 1yr at -70oC from date of shipment the E3 ubiquitin-protein ligases (2). Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized References and resuspended siRNAs are stored at 1. Gehrke, S. G. et.al: UbcH5A, a member of human E2 or below -20oC. After resuspension, ubiquitin-conjugating enzymes, is closely related to siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles SFT, a stimulator of iron transport, and is up-regulated without significant degradation. For in hereditary hemochromatosis. Blood 101: 3288-3293, long-term storage, it is recommended 2003. that the siRNA is stored at -70oC. For most favorable performance, avoid 2. Scheffner, M. et.al: Identification of a human repeated handling and multiple ubiquitin-conjugating enzyme that mediates the E6- freeze/thaw cycles. AP-dependent ubiquitination of p53. Proc. Nat. Acad. Sci. 91: 8797-8801, 1994.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.