21.10.2014YPEL3 antibody (pAb)

Rabbit Anti -Human/Mou se/Rat Yippee -like 3 (FKSG5)

Instruction Manual

Catalog Number PK-AB718-6775 Synonyms YPEL3 Antibody: Yippee-like 3, FKSG5 Description YPEL3 (yippee-like 3) belongs to a family of five yippee-like , all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence across species suggests that these proteins may have a conserved function involved in cell division. YPEL3 is a -regulated whose expression is induced by DNA damage and in turn induces cellular . It appears to function as a tumor suppressor as it is downregulated in colon and breast tumors. Quantity 100 µg Source / Host Rabbit Immunogen YPEL3 antibody was raised against a 15 synthetic peptide near the amino terminus of human YPEL3. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, ICC, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. YPEL3 antibody can be used for detection of YPEL3 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL. Images Available upon request. References Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43. Kelley K, Miller KR, Todd A, et al. YPEL3, a p53-regulated gene that induces . Res. 2010; 70:3566-75. Tuttle R, Simon R, Hitch DC, et al. Senescence-associated gene YPEL3 is downregulated in human tumors. Ann. Surg. Oncol. 2011; 18:1791-6. Tuttle R, Miller KR, Maiorano JN, et al. Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence. Int. J. Cancer 2011; epub. Images Available upon request. Related Products Cat. No. PK-AB718-6775P - YPEL3 Peptide Cat. No. PK-AB718-1288 - A-20 Cell Lysate

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014