Flt-3 Ligand Recombinant Cat. No.: 11-374

Flt-3 Ligand Recombinant Protein

Immobilized Human Flt-3, Fc Tag at 5 ug/mL (100 uL/well) can bind Mouse Flt-3 Ligand, His Tag with a linear range of 0.1-0.6 ng/mL (QC tested).

Specifications

SPECIES: Mouse

SOURCE SPECIES: HEK293 cells

SEQUENCE: Gly 27 - Arg 188

FUSION TAG: His Tag

TESTED APPLICATIONS: ELISA, WB

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW APPLICATIONS: of 20.2 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

PREDICTED MOLECULAR 20.2 kDa WEIGHT:

September 30, 2021 1 https://www.prosci-inc.com/flt-3-ligand-recombinant-protein-11-374.html Properties

PURITY: >90% as determined by SDS-PAGE.

PHYSICAL STATE: Lyophilized

BUFFER: PBS, pH7.4

Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon STORAGE CONDITIONS: reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: Flt3l

ALTERNATE NAMES: FLT3LG,FL,FLT3L,Flt3 ligand

ACCESSION NO.: NP_038548.3

GENE ID: 14256

Background and References

FMS-like 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the BACKGROUND: mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

REFERENCES: 1) Wodnar-Filipowicz A., 2003, News Physiol Sci., 18:247-51.

2) McClanahan T., et al., 1996, Blood, 88(9):3371-82.

3) Diener K.R. et al., 2008, Exp Hematol., 36(1):51-60.

4) Farag SS, Caligiuri MA, 2006, Blood Rev. 20(3):123-37.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.

September 30, 2021 2 https://www.prosci-inc.com/flt-3-ligand-recombinant-protein-11-374.html