DOCSLIB.ORG

Case Report of Granulicatella Adiacens As a Cause of Bacterascites

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Case Report of Granulicatella Adiacens As a Cause of Bacterascites Hindawi Publishing Corporation Case Reports in Infectious Diseases Volume 2015, Article ID 132317, 5 pages http://dx.doi.org/10.1155/2015/132317 Case Report Case Report of Granulicatella adiacens as a Cause of Bacterascites Molly C. Cincotta,1 K. C. Coffey,2 Shannon N. Moonah,3 Dushant Uppal,4 and Molly A. Hughes3 1 School of Medicine, University of Virginia, Charlottesville, VA 22908, USA 2Department of Medicine, University of Virginia, Charlottesville, VA 22908, USA 3Division of Infectious Diseases, Department of Medicine, University of Virginia, Charlottesville, VA 22908, USA 4Division of Gastroenterology, Department of Medicine, University of Virginia, Charlottesville, VA 22908, USA Correspondence should be addressed to Molly A. Hughes; [email protected] Received 29 July 2015; Revised 16 October 2015; Accepted 19 October 2015 Academic Editor: Paola Di Carlo Copyright © 2015 Molly C. Cincotta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Granulicatella adiacens is a Gram-positive coccus, formerly grouped with nutritionally variant Streptococcus,oftenfoundas commensal bacteria of the human oral cavity, urogenital tract, and gastrointestinal tract. Prior case reports have demonstrated Granulicatella spp. as a pathogen that can cause bacteremia and infective endocarditis particularly of prosthetic valves and pacemaker leads. Here, we report on a unique case of Granulicatella adiacens bacterascites in a 50-year-old male. 1. Introduction his PCP, who had been adjusting diuretics. However, the patient was failing diuretic management and so was referred Granulicatella adiacens is a Gram-positive coccus that is non- to our hospital for a therapeutic paracentesis. The patient’s motile, nonsporulating, catalase-negative, oxidase-negative, past medical history was significant for a complicated course and facultatively anaerobic. G. adiacens often grows on agar as of gallstone pancreatitis that occurred eight months prior to satellite colonies adjacent to another organism that provides his current presentation. Chart review revealed several pro- nutrients such as Staphylococcus aureus or Staphylococcus longed hospital courses, totaling 53 hospital days, with mul- epidermidis [1, 2]. G. adiacens colonies are alpha-hemolytic tiple complications, including necrotizing pancreatitis status on sheep-blood agar [1]. The organism has been found to be after necrosectomy and cholecystectomy, interval placement resistanttooptochinandsusceptibletovancomycin[1].The and subsequent removal of a percutaneous endoscopic gas- genus Granulicatella has three species, G. adiacens, G. elegans, trostomy tube with jejunal arm (PEG-J) for enteral feeding, and G. balaenopterae,withonlytheformertwohavingbeen Clostridium difficile diarrheal infection for which he had isolated from human samples [2]. Granulicatella species are received fourteen days of treatment with oral metronidazole, an uncommon cause of infection. To date, there have been and a new diagnosis of diabetes mellitus. Additionally, he no described cases of Granulicatella adiacens as a cause of hadbeenfoundtohavenewportalveinandsplenicvein spontaneous bacterial peritonitis or as an isolate of ascites thromboses during that hospitalization and had since com- fluid. pleted six months of therapeutic anticoagulation, originally with Apixaban, which was later switched to Rivaroxaban. 2. Case Report At the time of the patient’s current presentation for ascites, most of the above medical problems had resolved. He 2.1. History. A 50-year-old man presented to the emergency was tolerating a regular diet and was being followed by his department (ED) complaining of symptomatic large volume PCP for management of recurrent ascites felt to be due to the ascites. The patient and his ascites had been followed by above-mentioned portal vein thrombosis. 2 Case Reports in Infectious Diseases The patient reported no symptoms other than decreased pain, nausea, or diarrhea. His exam was unchanged from appetite secondary to bloating and pain in his right groin prior exam aside from a decrease in his abdominal distention. likely from nonincarcerated hernia. He had no fever, short- The GI team performed a diagnostic paracentesis, which ness of breath, nausea, vomiting, diarrhea, or significant revealed glucose of 135 mg/dL, WBC of 813/UL with 72% constipation. lymphocytes, 1% PMNs, and 2% atypical lymphocytes, total Upon presentation to the ED, computed tomography protein of 4.3 g/dL, and albumin of 2.1 g/dL. Ascites fluid (CT) of the abdomen showed large volume ascites with no again showed 3+ white blood cells and the bacterial culture propagation of the portal vein or splenic vein thromboses again grew Granulicatella adiacens. Peripheral blood cultures with interim development of collaterals. from two separate peripheral sites were collected and showed no growth after 72 hours. Blood work was not significantly 2.2. Physical Examination. On exam, the patient’s heart rate changed from the first presentation. wasnormalwitharegularrhythm,hislungswereclear, The patient underwent a transthoracic echocardiogram and there was no lower extremity edema. His abdomen was that showed no evidence of structural cardiac defect and no significantly distended, with bulging flanks and a palpable evidence of endocarditis. His previous CT was reexamined fluid wave. There was a well-healed midline surgical scar andalargefluidcollectionwasseenwithapossiblerim with a distal keloid and easily reducible umbilical hernia. of residual pancreatic tissue. Based on this, a magnetic res- Aside from the ascites, the patient had no other sequelae onance cholangiopancreatography (MRCP) was performed of chronic liver dysfunction; he exhibited no jaundice, no that revealed a fluid collection with possible communication palmar erythema, no spider angiomata, no venous distention, to the left colon. The GI team consulted the general surgery andnoasterixis. team, who, on review of the imaging and patient status, felt that the risks associated with any surgical intervention outweighed the potential benefits of source control. 2.3. Laboratory and Radiological Investigations. In the ED, Due to the patient having a penicillin allergy, he received the patient underwent paracentesis by the gastroenterology intravenous vancomycin for his course of therapy. The five- (GI) team under normal sterile technique with removal of day antibiotic course was initially complicated by an infusion- approximately 4.5 L of clear, yellow fluid. Initial Gram stain related “red man” reaction requiring pretreatment with of the ascites fluid revealed white blood cells and no bacteria. diphenhydramine and slower infusion times. On hospital Pertinent fluid analyses revealed glucose 100 mg/dL, amylase day 3, a peripherally inserted central venous catheter (PICC) 52U/L, WBC 372/UL with 48% polymorphonuclear cells line was placed, and the patient was discharged home in (PMNs) and 24% lymphocytes, total protein 4.2 g/dL, and stable condition. The patient underwent colonoscopy one albumin 2.1 g/dL giving a serum albumin ascites gradient of monthlaterandwasfoundtohavethreehyperplasticpolyps 1.5, consistent with portal hypertension or congestive heart without evidence of malignancy. There was no apparent failure. Blood work revealed a peripheral white blood cell communication between the colon and the fluid pocket that count of 6.85 k/ L with a normal differential, platelet count had been visualized on MRCP. No further blood or ascites of 326 k/ L, and a lactic acid of 1.3 mmol/L. Ultrasound of the cultures were collected in the immediate period after his abdomen confirmed that there was no interim propagation hospitalization. of the portal vein thrombosis as well as presence of patent collaterals. 3. Discussion 2.4. Hospital Course. The patient was discharged from the ED Granulicatella adiacens is an uncommon cause of infec- with a follow-up outpatient appointment with a GI physician tion, but when present, it is most commonly found in for further evaluation and management of recurrent ascites. the bloodstream. Review of the English literature shows The following day, the GI team was contacted by the Clinical that bacteremia is usually related to device/graft infection Microbiology Laboratory that Gram-positive cocci in chains or endocarditis (Table 1). Cargill et al. described 17 cases were growing in the ascites sample. The GI team contacted the of Granulicatella spp. endocarditis between 1997 and 2012 patient’s wife who reported that the patient had experienced including infections of prosthetic valves and a pacemaker no clinical status change since the procedure, specifically no lead [2]. It is suspected that cases of Granulicatella spp. fevers or abdominal pain. infective endocarditis are underreported and that some cases The GI team felt that the laboratory result likely rep- may be a cause of reported culture-negative endocarditis. resented a contaminant in the sample, and they decided Bacteremia with Granulicatella spp.without endocarditis has to await speciation before asking the patient to return to been reported, including a single case of infection of aortic the hospital. The following day, the Clinical Microbiology atheroma with associated dissection [2]. Other infections Laboratory reported Granulicatella adiacens (not a common have included seeding by prosthetic material or surgery with laboratory contaminant),
Load more

Recommended publications
  • The Oral Microbiome of Healthy Japanese People at the Age of 90
    applied sciences Article The Oral Microbiome of Healthy Japanese People at the Age of 90 Yoshiaki Nomura 1,* , Erika Kakuta 2, Noboru Kaneko 3, Kaname Nohno 3, Akihiro Yoshihara 4 and Nobuhiro Hanada 1 1 Department of Translational Research, Tsurumi University School of Dental Medicine, Kanagawa 230-8501, Japan; [email protected] 2 Department of Oral bacteriology, Tsurumi University School of Dental Medicine, Kanagawa 230-8501, Japan; [email protected] 3 Division of Preventive Dentistry, Faculty of Dentistry and Graduate School of Medical and Dental Science, Niigata University, Niigata 951-8514, Japan; [email protected] (N.K.); [email protected] (K.N.) 4 Division of Oral Science for Health Promotion, Faculty of Dentistry and Graduate School of Medical and Dental Science, Niigata University, Niigata 951-8514, Japan; [email protected] * Correspondence: [email protected]; Tel.: +81-45-580-8462 Received: 19 August 2020; Accepted: 15 September 2020; Published: 16 September 2020 Abstract: For a healthy oral cavity, maintaining a healthy microbiome is essential. However, data on healthy microbiomes are not sufficient. To determine the nature of the core microbiome, the oral-microbiome structure was analyzed using pyrosequencing data. Saliva samples were obtained from healthy 90-year-old participants who attended the 20-year follow-up Niigata cohort study. A total of 85 people participated in the health checkups. The study population consisted of 40 male and 45 female participants. Stimulated saliva samples were obtained by chewing paraffin wax for 5 min. The V3–V4 hypervariable regions of the 16S ribosomal RNA (rRNA) gene were amplified by PCR.
    [Show full text]
  • Granulicatella Adiacens Bacteria Isolation from Perodontitical
    Sys Rev Pharm 2020; 11(4): 396 400 A multifaceted review journal in the field of pharmacy E-ISSN 0976-2779 P-ISSN 0975-8453 Granulicatella Adiacens Bacteria Isolation from Perodontitical Patients with Polymerase Chain Reaction Techniques Harun Achmad1, Sri Oktawati2,Andi Mardiana Adam2,Burhanuddin Pasiga3, Rizalinda Sjahril4, Aulia Azizah5, Bayu Indra Sukmana6, Huldani7, Heri Siswanto8 , Ingrid Neormansyah8 1Lecturer of Pedodontics Department, Dentistry Faculty, Hasanuddin University, Makassar, Indonesia 2Lecturer of Periodontology Department, Dentistry Faculty, Hasanuddin University, Makassar, Indonesia 3Lecturer of Dental Public Health Department, Dentistry Faculty, Hasanuddin University, Makassar, Indonesia 4 Lecturer of Microbiology Department, Medical Faculty, Hasanuddin University, Makassar, Indonesia 5Department of Preventive and Public Health Dentistry, Faculty of Dentistry, Lambung Mangkurat University, Banjarmasin, Indonesia. 6Department of Dental Radiology, Faculty of Dentistry, Lambung Mangkurat University, Banjarmasin, Indonesia. 7Department of Physiology, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia. 8 Resident of Periodontology Department, Dentistry Faculty, Hasanuddin University, Makassar, Indonesia Correspondence Author E-mail: [email protected] Article History: Submitted: 10.01.2020 Revised: 12.03.2020 Accepted: 24.04.2020 ABSTRACT Background: Periodontitis is an inflammatory disease of dental support the NCBI Gene Bank using BLAST analysis. tissue caused by certain groups of microorganisms,
    [Show full text]
  • Bacterial Diversity and Functional Analysis of Severe Early Childhood
    www.nature.com/scientificreports OPEN Bacterial diversity and functional analysis of severe early childhood caries and recurrence in India Balakrishnan Kalpana1,3, Puniethaa Prabhu3, Ashaq Hussain Bhat3, Arunsaikiran Senthilkumar3, Raj Pranap Arun1, Sharath Asokan4, Sachin S. Gunthe2 & Rama S. Verma1,5* Dental caries is the most prevalent oral disease afecting nearly 70% of children in India and elsewhere. Micro-ecological niche based acidifcation due to dysbiosis in oral microbiome are crucial for caries onset and progression. Here we report the tooth bacteriome diversity compared in Indian children with caries free (CF), severe early childhood caries (SC) and recurrent caries (RC). High quality V3–V4 amplicon sequencing revealed that SC exhibited high bacterial diversity with unique combination and interrelationship. Gracillibacteria_GN02 and TM7 were unique in CF and SC respectively, while Bacteroidetes, Fusobacteria were signifcantly high in RC. Interestingly, we found Streptococcus oralis subsp. tigurinus clade 071 in all groups with signifcant abundance in SC and RC. Positive correlation between low and high abundant bacteria as well as with TCS, PTS and ABC transporters were seen from co-occurrence network analysis. This could lead to persistence of SC niche resulting in RC. Comparative in vitro assessment of bioflm formation showed that the standard culture of S. oralis and its phylogenetically similar clinical isolates showed profound bioflm formation and augmented the growth and enhanced bioflm formation in S. mutans in both dual and multispecies cultures. Interaction among more than 700 species of microbiota under diferent micro-ecological niches of the human oral cavity1,2 acts as a primary defense against various pathogens. Tis has been observed to play a signifcant role in child’s oral and general health.
    [Show full text]
  • Atypica, and Granulicatella Adiacens in Biofilm of Complete Dentures
    [Downloaded free from http://www.j-ips.org on Thursday, November 1, 2018, IP: 183.82.145.117] Original Article Relative presence of Streptococcus mutans, Veillonella atypica, and Granulicatella adiacens in biofilm of complete dentures Binoy Mathews Nedumgottil Department of Prosthodontics and Implantology, Mahe Institute of Dental Sciences and Hospital, Puducherry, India Abstract Aims and Objective: Oral biofilms in denture wearers are populated with a large number of bacteria, a few of which have been associated with medical conditions such as sepsis and infective endocarditis (IE). The present study was designed to investigate the relative presence of pathogenic bacteria in biofilms of denture wearers specifically those that are associated with IE. Methods: Biofilm samples from 88 denture wearers were collected and processed to extract total genomic DNA. Eight of these samples were subjected to 16S rRNA gene sequencing analysis to first identify the general bacterial occurrence pattern. This was followed by species-specific quantitative polymerase chain reaction (qPCR) on entire batch of 88 samples to quantify the relative copy numbers of IE-associated pathogens. Results: 16S rRNA gene analysis of eight biofilm samples identified bacteria from Firmicutes, Actinobacteria, Proteobacteria, Bacteroidetes, and Fusobacteria species. Interestingly, Streptococcus mutans, Veillonella atypica, and Granulicatella adiacens from Firmicutes, all known to be associated with early-onset sepsis and IE was present in five of eight biofilm samples. The other three samples carried bacteria from genus Proteobacteria with Neisseria flava and Neisseria mucosa, which are known to be commensals, as dominant species. Species-specific qPCR of S. mutans V. atypica, and G. adiacens on 88 biofilm DNA samples identified the presence of S.
    [Show full text]
  • Identification of Clinically Relevant Streptococcus and Enterococcus
    pathogens Article Identification of Clinically Relevant Streptococcus and Enterococcus Species Based on Biochemical Methods and 16S rRNA, sodA, tuf, rpoB, and recA Gene Sequencing Maja Kosecka-Strojek 1,* , Mariola Wolska 1, Dorota Zabicka˙ 2 , Ewa Sadowy 3 and Jacek Mi˛edzobrodzki 1 1 Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland; [email protected] (M.W.); [email protected] (J.M.) 2 Department of Molecular Microbiology, National Medicines Institute, 00-725 Warsaw, Poland; [email protected] 3 Department of Epidemiology and Clinical Microbiology, National Medicines Institute, 00-725 Warsaw, Poland; [email protected] * Correspondence: [email protected]; Tel.: +48-12-664-6365 Received: 13 October 2020; Accepted: 9 November 2020; Published: 11 November 2020 Abstract: Streptococci and enterococci are significant opportunistic pathogens in epidemiology and infectious medicine. High genetic and taxonomic similarities and several reclassifications within genera are the most challenging in species identification. The aim of this study was to identify Streptococcus and Enterococcus species using genetic and phenotypic methods and to determine the most discriminatory identification method. Thirty strains recovered from clinical samples representing 15 streptococcal species, five enterococcal species, and four nonstreptococcal species were subjected to bacterial identification by the Vitek® 2 system and Sanger-based sequencing methods targeting the 16S rRNA, sodA, tuf, rpoB, and recA genes. Phenotypic methods allowed the identification of 10 streptococcal strains, five enterococcal strains, and four nonstreptococcal strains (Leuconostoc, Granulicatella, and Globicatella genera). The combination of sequencing methods allowed the identification of 21 streptococcal strains, five enterococcal strains, and four nonstreptococcal strains.
    [Show full text]
  • Insights Into 6S RNA in Lactic Acid Bacteria (LAB) Pablo Gabriel Cataldo1,Paulklemm2, Marietta Thüring2, Lucila Saavedra1, Elvira Maria Hebert1, Roland K
    Cataldo et al. BMC Genomic Data (2021) 22:29 BMC Genomic Data https://doi.org/10.1186/s12863-021-00983-2 RESEARCH ARTICLE Open Access Insights into 6S RNA in lactic acid bacteria (LAB) Pablo Gabriel Cataldo1,PaulKlemm2, Marietta Thüring2, Lucila Saavedra1, Elvira Maria Hebert1, Roland K. Hartmann2 and Marcus Lechner2,3* Abstract Background: 6S RNA is a regulator of cellular transcription that tunes the metabolism of cells. This small non-coding RNA is found in nearly all bacteria and among the most abundant transcripts. Lactic acid bacteria (LAB) constitute a group of microorganisms with strong biotechnological relevance, often exploited as starter cultures for industrial products through fermentation. Some strains are used as probiotics while others represent potential pathogens. Occasional reports of 6S RNA within this group already indicate striking metabolic implications. A conceivable idea is that LAB with 6S RNA defects may metabolize nutrients faster, as inferred from studies of Echerichia coli.Thismay accelerate fermentation processes with the potential to reduce production costs. Similarly, elevated levels of secondary metabolites might be produced. Evidence for this possibility comes from preliminary findings regarding the production of surfactin in Bacillus subtilis, which has functions similar to those of bacteriocins. The prerequisite for its potential biotechnological utility is a general characterization of 6S RNA in LAB. Results: We provide a genomic annotation of 6S RNA throughout the Lactobacillales order. It laid the foundation for a bioinformatic characterization of common 6S RNA features. This covers secondary structures, synteny, phylogeny, and product RNA start sites. The canonical 6S RNA structure is formed by a central bulge flanked by helical arms and a template site for product RNA synthesis.
    [Show full text]
  • Nutritionally Variant Streptococci)
    Microbiol. Immunol., 44(12), 981-985, 2000 Serological Properties of Abiotrophia and Granulicatella Species (Nutritionally Variant Streptococci) Katsuhiro Kitada, Yasuko Okada, Taisei Kanamoto*, and Masakazu Inoue* Department of Preventive Dentistry, Kagoshima University Dental School, Kagoshima, Kagoshima 890-8544, Japan Received May 11, 2000; in revised form, August 15, 2000. Accepted September 9, 2000 Abstract: Serological variations were examined among 12 type or reference strains and 91 oral isolates of vitamin B6-dependent Abiotrophia and Granulicatella spp. Rabbits were immunized with whole cells of 12 selected strains and 10 typing antisera were obtained, which were unreactive with the Lancefield group A to G antigen preparations. The reactivity of the antisera and autoclaved cell surface antigen extracts was tested by double diffusion in agar gel and a capillary precipitin test. These typing antisera categorized all Abiotrophia defectiva strains, all except one Granulicatella elegans strain, three-quarters of the Granulicatella adiacens, and half of the Granulicatella paraadiacens into 8 serotypes and 2 subserotypes. The Granulicatella balaenopterae type strain was unserotypable. All A. defectiva strains were serotype I, some of which were divided into subserotype I-1 and/or I-5. The G. adiacens strains generally belonged to serotype II or III, and the G. paraadiacens strains to serotype IV, V or VI. All G. adiacens or G. paraadiacens serotype II strains were also subserotype 1-5. The G. elegans strains were serotype VII or VIII. These Abiotrophia and Gran- ulicatella serotypes were undetectable among 33 strains of the other 11 species including the bacteriolytic enzyme-producing but vitamin B6-independent strains of Streptococcus, Enterococcus, Dolosigranulum and Aerococcus.
    [Show full text]
  • Granulicatella Adiacens, an Unusual Causative Agent in Chronic Dacryocystitis
    Faculty & Staff Scholarship 2015 Granulicatella adiacens, an unusual causative agent in chronic dacryocystitis Cristy A. Ku Blake Forcina Paul R. LaSala John Nguyen Follow this and additional works at: https://researchrepository.wvu.edu/faculty_publications Ku et al. Journal of Ophthalmic Inflammation and Infection (2015) 5:12 DOI 10.1186/s12348-015-0043-2 BRIEF REPORT Open Access Granulicatella adiacens, an unusual causative agent in chronic dacryocystitis Cristy A Ku1, Blake Forcina2, Paul Rocco LaSala3 and John Nguyen2* Abstract Background: Granulicatella adiacens, a recent taxonomic addition, is a commensal organism of the oral, gastrointestinal, and urogenital tracts and is rarely encountered in the orbit and eye. Findings: We present a 46-year-old Caucasian woman with chronic dacryocystitis who underwent an external dacryocystorhinostomy and was found to have G. adiacens. Conclusions: This is an unusual causative organism isolated in the nasolacrimal system and, to our knowledge, the first reported case of chronic dacryocystitis associated with G. adiacens. Keywords: Granulicatella adiacens; Chronic; Dacryocystitis; Dacryocystorhinostomy; Nasolacrimal duct obstruction Findings pressure was 15 mmHg in each eye. Pooling of clear Introduction tears was noted on a normal-appearing right lower lid Acquired nasolacrimal duct obstruction (NLDO), a com- without an enlarged, erythematous, or tender lacrimal mon cause of epiphora and dacryocystitis, is typically sac. Nasolacrimal dilation and irrigation revealed complete managed with dacryocystorhinostomy (DCR). Even in reflux of clear saline, and the remainder of the ocular cases without signs of infection, positive bacterial cultures exam was unremarkable. are often obtained from the lacrimal sac [1]. Gram- The patient underwent an uneventful external dacryo- positive bacteria - Staphylococcus epidermidis, Staphylo- cystorhinostomy with placement of Crawford stent.
    [Show full text]
  • Granulicatella Spp. Diversas Comunicaciones Han Vinculado a Granulicatella Spp
    Comunicación Breve Granulicatella spp. Diversas comunicaciones han vinculado a Granulicatella spp. como responsable de diversos cuadros clínicos que difieren de su presentación César Reyes y M. Elizabeth Barthel habitual. Es así como existen datos de que en el grupo de las EI causadas por NVS existe una alta negatividad de los cultivos (41%), recaídas ma- yores a las habituales (17%), y una mortalidad de 17%12. Sin embargo, en los últimos reportes esa cifra ha descendido a 9%13, básicamente por la Granulicatella spp. is a bacteria of the oral cavity, belonging to the mayor disponibilidad de técnicas moleculares que permite identificar al nutritionally variant group streptococci, and has been identified in 5% of 5 all bacterial endocarditis. It’s an important etiologic species in endocar- microorganismo en las vegetaciones y así dirigir adecuadamente la terapia . ditis, particularly in the setting of negative blood cultures. Granulicatella Habitualmente la historia de EI por NVS está precedida de un procedimiento is a non-mobile, non- spore forming organism that is both catalase and dental meses antes, con o sin profilaxis antibacteriana. En total, diecisiete oxidase negative. The treatment for Granulicatella, is the same for En- casos de EI por Granulicatella han sido descritos en la literatura científica terococcus according to the American and European guidelines, however desde 1997, incluyendo válvulas protésicas y marcapasos. Granulicatella resistance to this treatment has been reported. adiacens es el microorganismo más frecuentemente aislado, siendo G. 10 Key words: Granulicatella, nutritionally variant streptococci, endo- elegans un hallazgo infrecuente . carditis. Si bien la EI es la presentación clínica no odontológica más frecuente, 10,14-16 Palabras clave: Granulicatella, Streptococcus de variante nutricional, también se ha asociado a bacteriemias no asociadas a endocarditis , 17 endocarditis.
    [Show full text]
  • Post-Operative Endophthalmitis Caused by the Nutritionally Variant Streptococcus Granulicatella Adiacens
    perim Ex en l & ta a l ic O p in l h t C h f Journal of Clinical & Experimental a o l m l a o n l r o Todokoro, J Clin Exp Ophthalmol 2016, 7:3 g u y o J Ophthalmology 10.4172/2155-9570.1000557 ISSN: 2155-9570 DOI: Case Report Open Access Post-Operative Endophthalmitis Caused by the Nutritionally Variant Streptococcus Granulicatella adiacens Daisuke Todokoro1*, Kiyofumi Mochizuki2, Kiyofumi Ohkusu3, Ryuichi Hosoya4, Nobumichi Takahashi2, and Shoji Kishi1,5 1Department of Ophthalmology, Gunma University School of Medicine, Japan 2Department of Ophthalmology, Gifu University School of Medicine, Japan 3Department of Microbiology, Tokyo Medical University, Japan 4Department of Clinical Laboratory, Gunma University School of Medicine, Japan 5Maebashi Central Eye Clinic, Japan *Corresponding author: Daisuke Todokoro, Department of Ophthalmology, Gunma University School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma, Japan, Tel: +81-27-220-8338; Fax: +81-27-233-3841; E-mail: [email protected] Received date: Apr 27, 2016; Accepted date: May 25, 2016; Published date: May 30, 2016 Copyright: © 2016 Todokoro D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Objective: Bacterial endophthalmitis is among the most serious ocular infections. Nutritionally variant streptococci (NVS) are bacteria that are difficult to culture with standard media because of fastidious growth requirements. The cases of post-operative endophthalmitis caused by Granulicatella adiacens, one of the NVS were reported.
    [Show full text]
  • Case Report Granulicatella Adiacens and Abiotrophia Defectiva Native
    Hindawi Case Reports in Infectious Diseases Volume 2019, Article ID 5038563, 8 pages https://doi.org/10.1155/2019/5038563 Case Report Granulicatella adiacens and Abiotrophia defectiva Native Vertebral Osteomyelitis: Three Cases and Literature Review of Clinical Characteristics and Treatment Approach Cinzia Puzzolante ,1 Gianluca Cuomo ,1 Marianna Meschiari,1 Andrea Bedini,1 Aurora Bonazza,1 Claudia Venturelli ,2 Mario Sarti,3 and Cristina Mussini1,4 1Azienda Ospedaliero-Universitaria di Modena, Infectious Disease Clinic, Modena, Italy 2Clinical Microbiology, Azienda Ospedaliero-Universitaria di Modena, Modena, Italy 3Clinical Microbiology, Ospedale Civile di Baggiovara, Modena, Italy 4University of Modena and Reggio Emilia, Azienda Ospedaliero-Universitaria di Modena, Infectious Disease Clinic, Modena, Italy Correspondence should be addressed to Cinzia Puzzolante; [email protected] Received 8 January 2019; Accepted 16 April 2019; Published 6 May 2019 Academic Editor: Antonella Marangoni Copyright © 2019 Cinzia Puzzolante et al. 'is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Granulicatella adiacens and Abiotrophia defectiva are an increasingly recognized cause of osteoarticular infections. We describe two cases of G. adiacens and one case of A. defectiva native vertebral osteomyelitis (NVO) and review all published cases. Nine cases of G. adiacens NVO and two cases of A. defectiva NVO were previously described. Patients were usually middle-aged men, and classical risk factors for NVO were present in half of the cases. Concomitant bacteremia was reported in 78.6% of cases, and concurrent infective endocarditis occurred in 36.4% of this sub-group of patients.
    [Show full text]
  • Challenge M142-4 CMPT Clinical Bacteriology Program
    CMPT Clinical Bacteriology Program Innovation, Education, Quality Assessment, Continual Improvement www.cmpt.ca Challenge M142-4 August 2014 Blood culture: Granulicatella adiacens (Nutritionally variant streptococci) HISTORY MAIN EDUCATIONAL POINTS from M142-4 A simulated blood culture obtained from a 52 1. Granulicatella adiacens and other nutritionally variant streptococ- year old male in-patient with a heart murmur ci (e.g. Abiotrophia defectiva, other Granulicatella species) are was sent to category A laboratories. infrequent but important isolates from blood cultures. Participants were expected to isolate and iden- 2. Media or techniques (e.g. chocolate agar or a streak of Staphylo- tify Granulicatella adiacens. coccus aureus) that allow for these organisms to be isolated from blood cultures should be routinely used. CMPT QA/QC 3. The Gram stain may appear quite pleomorphic, but should not be CMPT control yielded a pure culture of Granu- mistaken for bacilli or for gram negative organisms. licatella adiacens, viable for 8 days. 4. Correct identification to a genus or species level may be achieved using identification systems in the laboratory. However, even in SURVEY RESULTS their absence, it is important for a laboratory to have the ability to cultivate and recognize a nutritionally variant streptococci. Reference Laboratories: 14/15 (93%) laborato- ries reported G. adiacens, Granulicatella spe- cies, or nutritionally variant streptococci (NVS). Taxonomically, Abiotrophia species is not cor- Grading One laboratory reported results using the incor- rect (discussed below), but was considered rect identifier. Consensus was reached, there- sufficiently close to merit partial grading. Maximum grade: 4 fore the challenge was considered suitable for ISOLATION and IDENTIFICATION Reporting Granulicatella, grading.
    [Show full text]