Flt3 Ligand Recombinant (HEK293 Expressed) Cat. No.: 96-321

Flt3 Ligand Recombinant Protein (HEK293 Expressed)

Specifications

SPECIES: Human

SOURCE SPECIES: HEK293 cells

SEQUENCE: Thr 27 - Pro 185

FUSION TAG: Tag free

TESTED APPLICATIONS: WB

APPLICATIONS: This recombinant protein can be used for WB. For research use only.

PREDICTED MOLECULAR 18.9 kDa WEIGHT:

Properties

PURITY: >95% as determined by SDS-PAGE.

PHYSICAL STATE: Lyophilized

BUFFER: PBS, pH7.4

September 25, 2021 1 https://www.prosci-inc.com/flt3-ligand-recombinant-protein-hek293-expressed-96-321.html Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon STORAGE CONDITIONS: reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: FLT3LG

ALTERNATE NAMES: FLT3LG, FL, FLT3L, Flt3 ligand

ACCESSION NO.: AAI26294

GENE ID: 2323

Background and References

FMS-like 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the BACKGROUND: mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

REFERENCES: 1) Wodnar-Filipowicz A., 2003, News Physiol Sci., 18:247-51.

2) McClanahan T., et al., 1996, Blood, 88(9):3371-82.

3) Diener K.R. et al., 2008, Exp Hematol., 36(1):51-60.

4) Farag SS, Caligiuri MA, 2006, Blood Rev. 20(3):123-37.

ANTIBODIES FOR RESEARCH USE ONLY.

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