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Soluble and Cell Wall Bound Peroxidase Activities Are Markers of Flower Bud Development Stages in Lemon (Citrus Limon L.) M.F

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Soluble and Cell Wall Bound Peroxidase Activities Are Markers of Flower Bud Development Stages in Lemon (Citrus Limon L.) M.F Journal of Applied Botany and Food Quality 87, 1 - 8 (2014), DOI:10.5073/JABFQ.2014.087.001 1 Université Paul Sabatier, Centre d’Etudes Spatiales de la Biosphère (CESBIO, UMR 5126), Toulouse, France 2 VetAgro Sup, campus agronomique Clermont, Lempdes, France 3 Université Paul Sabatier, Département de Langues et Gestion, Toulouse, France Soluble and cell wall bound peroxidase activities are markers of flower bud development stages in lemon (Citrus limon L.) M.F. Kasraoui1, I. Duquesnoy2, P. Winterton3, T. Lamaze*1 (Received September 5, 2013) Summary spring (following the inductive cold winter season (GAR C IA -LUIS and KAN D USER , 1995) and plays the major role in yield. The second Specific activities of H O scavenging enzymes, soluble peroxidase 2 2 corresponds to the autumn-flush growth. However, the lemon tends (G-POD), catalase (CAT) and ascorbate peroxidase (APX), and cell to flower continuously throughout the year in cooler, coastal climates wall bound (CWB) G-POD, have been characterized in flower buds where trees can produce several crops each year. Flowering shoots and leaves in potted lemon trees (Citrus limon L. Burm.) growing are often produced on woody twigs of the previous year’s spring-flush in a glasshouse in Tunisia. In leaves during the winter rest period of growth but may also be produced on summer-flush twigs or on (from September to December), enzyme activities did not change older wood (SPIE G E L -ROY and GO ld S C HMI D T , 1996). They may form markedly. Then, leaf G-POD (soluble and CWB activity) increased only flowers (generative shoots also called leafless inflorescences) or 4-5 fold in January as the buds reached dormancy release. In growing both flowers and leaves (mixed-type inflorescences). buds, the activities of the three enzymes increased strongly (10- to 100-fold) between dormancy and full bloom and decreased slightly (3-fold) post-bloom (beginning of fruit-set). Along the shoots, the In aerobic life, oxygen is subject to in vivo activation into toxic forms developmental stages of flower buds advanced from node 1 to node during normal cell metabolism. Reactive oxygen species (ROS) are partially reduced oxygen intermediates. The most damaging forms of 5. G-POD activity increased from the shoot apex toward the base - ROS are superoxide radicals (*O 2), hydroxyl radicals (*OH), singlet of the twig in both leaves and buds. A similar trend was observed 1 for APX and CAT but their activities tended to decrease slightly oxygen ( O2) and hydrogen peroxide (H2O2) and ROS are generated farther from the apex. Retardation of flowering by application during metabolic conversion of molecular oxygen (CERUTTI , 1991). of growth regulators strongly reduced leaf-specific activities of In plants, the production of ROS is drastically increased in response to biotic and abiotic stresses. The ROS may be very damaging since the H2O2 scavenging enzymes. Therefore, G-POD activity (and to a lesser extent APX and CAT) appears to be a good marker of they can oxidize a variety of organic molecules such as proteins, flower formation in lemon (from bud swelling to full bloom). The lipids and DNA (PIETTA , 2000) so their removal is essential. Plants possess well-defined antioxidative systems to scavenge ROS, results also suggest a link between H2O2 production and flower bud development. including low-molecular weight compounds and enzymes (HOSSAIN et al., 2006). Superoxide dismutase, SOD (EC 1.15.1.1) detoxifies - *O 2 by converting it into O2 and H2O2. While H2O2 is not itself a Abbreviations free-radical, it can generate highly reactive *OH radicals. Catalase, CWB: Cell wall bound; ROS: Reactive oxygen species; G-POD: CAT (EC 1.11.1.6) catalyses H2O2 breakdown into H2O and O2. Guaiacol peroxidase; APX: Ascorbate peroxidase; CAT: Catalase; Peroxidases, POD (EC 1.11.1.7), catalyse oxido-reduction between H2O2 and various reductants with low substrate specificity; ascorbate GA3: Gibberellic acid; MH: Maleic hydrazide. peroxidase, APX (EC 1.11.1.11) also degrades H2O2 requiring ascorbic acid as electron donor. Introduction APX, CAT and POD are among the most important enzymes in H2O2 scavenging. APX acts as a central component of the major Flowering is an essential phenomenon in the life cycle of higher hydrogen peroxide detoxifying system in plant cells: the ascorbate plants, being the central process of species reproduction. The for- – glutathione cycle, and plays an essential role in the control of mation of flower buds is also a fundamental factor of productivity. intracellular ROS levels (ROSA et al., 2010). CAT participates The change from vegetative to reproductive growth is called the in the main defense system against the accumulation and toxicity flowering transition. It is controlled by inheritance, internal and of hydrogen peroxide and plays a central role in controlling H2O2 external factors and is accompanied by various changes in the levels in the cell, therefore controlling the regulation of the signal biochemistry, physiology, cytology and morphology of the buds transduction pathway that is dependent on ROS (STARZYNS K A leading to the formation of reproductive structures (CHEN et al., et al. 2003). It has been proposed that the levels of POD are strongly 2009). The lemon (Citrus limon L.) is an economically important modulated in cells since they are involved in growth, development crop consumed worldwide. The flowering process in citrus consists and senescence processes in plants and vary in response to both of several discrete phases: flower bud induction, bud differentiation biotic and abiotic stresses (NORIE G A et al., 2007). In recent years, and anthesis. The induction process is dependent on environmental there has been growing interest in the functional significance of and endogenous (such as fruit load) factors, and results in the ROS in plant growth and development. ROS are known not only commitment of meristematic cells to form reproductive structures. for their hazardous oxidative damage effects but also for their role Floral differentiation involves the anatomical and morphological in modulating both signaling and defense functions. It has been AVENPORT transition of vegetative meristems to floral meristems D( , shown that H2O2 acts as a development signaling molecule (TIAN 1990). In citrus, the differentiation of floral organs begins during et al., 2003) and that H2O2 metabolism coordination in different the first stages of bud swelling and sprouting. Lemon trees have sub-cellular compartments is complex since it can diffuse between two major flowering periods in Mediterranean climates. One is in compartments (ROSA et al., 2010). GONZA L EZ et al. (1991) and LE G RAN D and BOUAZZA (1991) showed * Corresponding author that specific peroxidase isoenzymes were related to organogenesis. 2 M.F. Kasraoui, I. Duquesnoy, P. Winterton, T. Lamaze WAN G et al. (1991, (a) and (b)) examined ‘York Imperial’ apple activity at various stages of flower bud development and fruit-set vegetative bud development prior to flowering. They observed an were followed in buds sampled at the beginning of March on the increase in catalase activities during bud swelling and then a decline first node of the tip of twigs formed the previous year. Changes in at bud break. These authors also found high POD activity in dormant protein content and enzyme activities in leaves and young growing buds, which then declined during bud break to increase again during flower buds according to the position along the shoot were assayed in bud growth. The works of BERNA L et al. (1993) reveal that Capsicum young growing buds and younger fully expanded leaves sampled at annuum flowering is regulated by internal factors associated with an the beginning of March from the tip (first insertion level, first node) increase in peroxidase activity in the leaves. AB ASSI et al. (1998), to the fifth node below the apex (insertion level 5). evaluating Red Spur Delicious flower bud development, report In many fruit trees, flowering can be inhibited by the application that changes in catalase and peroxidase activities were related to of exogenous GA3. MH is a synthetic compound which has also a growth and development of the apple flower. Also, MONERRI and plant growth regulating action. It has long been used in agriculture GUAR D IO L A (2001) showed that changes in peroxidase activity as a depressant of plant growth which retards flowering and prolongs occur concomitantly with floral transition in Citrus unshiu. HUF F dormancy. A separate experiment was conducted to test the influence and DY B IN G (1980), in the soybean (Glycine max) observed that of GA3 and MH on changes in leaf enzyme activities associated with peroxidase activity changes in parallel with ovary development, the retarding effect on flowering. Retardant sprays or water sprays increasing rapidly in growing pods but not in shedding flowers. (control) were applied to whole trees (5 plants per treatment) on 15th Peroxidases could be involved in numerous processes associated January (i.e. about 2-3 weeks before normal blooming). Treated- with flower bud development: lignification (WHETTEN et al., 1998), trees were sprayed to the drip point with GA3 concentrations of 50 suberization (ESPE L IE et al., 1986), cross-linking of cell wall or 100 ppm and MH concentrations of 1000 or 1500 ppm (plus 0.2% structural proteins (FRY , 1986), auxin catabolism (LA G RIMINI et al., Tween-80). Responses to GA3 and MH were recorded in leaves at the 1997) and tissue senescence (AB E L ES et al., 1988). beginning of February. In addition, identical treatments were applied The literature thus suggests that the enzymes which degrade H2O2 to lemon cultivars other than “Eureka” (Femminello, Kutdeken, (in particular POD) play an important role in protecting plant tissue Santateresa and Interdonato) on 5-year-old trees cultivated outdoors during flower-bud development.
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