Rabbit Anti-GCF2/LRRFIP1/FITC Conjugated Antibody

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Rabbit Anti-GCF2/LRRFIP1/FITC Conjugated antibody

SL12439R-FITC

Product Name: Anti-GCF2/LRRFIP1/FITC Chinese Name: FITC标记的巨噬细胞调控相关蛋白LRRFIP1抗体 FLAP 1; FLAP1; FLIIAP 1; FLIIAP1; GC binding factor 2; GC-binding factor 2; GCF 2; GCF2; HUFI 1; HUFI1; Leucine rich repeat (in FLII) interacting protein 1; Leucine rich repeat flightless interacting protein 1; Leucine-rich repeat flightless-interacting Alias: protein 1; LRR FLII interacting protein 1; LRR FLII-interacting protein 1; LRRF1_HUMAN; LRRFIP 1; Lrrfip1; MGC10947; MGC119738; MGC119739; NEDD8 conjugating enzyme; TAR RNA interacting protein; TAR RNA-interacting protein; Transcription factor 9 like; TRIP. Organism Species: Rabbit Clonality: Polyclonal React Species: Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep, ICC=1:50-200IF=1:50-200 Applications: not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. Molecular weight: 89kDawww.sunlongbiotech.com Form: Lyophilized or Liquid Concentration: 1mg/ml immunogen: KLH conjugated synthetic peptide derived from human LRRFIP1 Lsotype: IgG Purification: affinity purified by Protein A Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year Storage: when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. background: Product Detail: LRRFIP1 is an 738 amino acid human protein whose rodent counterpart is known as Lrrfip1 (also designated FLAP in mouse). LRRFIP1 is also believed to control smooth muscle cell proliferation following arterial injury through PDGF-A repression. The N- terminus of LRRFIP1 shows high homology to the coiled-coil domain of FLAP, a protein which binds the leucine-rich repeat (LRR) of Flightless I, and the interaction of LRRFIP1 with the LRR of Flightless I has been confirmed. LRRFIP1 does not bind single-stranded DNA or RNA significantly and binds double-stranded DNA weakly. In contrast, LRRFIP1 binds double-stranded RNA with high affinity, and two molecules of LRRFIP1 bind the TaR stem. The RNA binding domain has been identified and encompasses a lysine-rich motif. Flightless I has a C-terminal TaR-like domain which binds Actin and therefore the association of LRRFIP1 with the LRR of Flightless I may provide a link between the Actin cytoskeleton and RNA in mammalian cells.

Function: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA.

Subunit: Interacts with FLII. Interacts with MYD88. Competes with FLII for MyD88-binding, even in the absence of LPS.

Subcellular Location: Nucleus. Cytoplasm.

Tissue Specificity: Ubiquitously expressed.

Similarity: Belongs to the LRRFIP family.

Database links:

Entrezwww.sunlongbiotech.com Gene: 100066467 Horse

Entrez Gene: 9208 Human

Entrez Gene: 16978 Mouse

Entrez Gene: 367314 Rat

Omim: 603256 Human

SwissProt: Q32MZ4 Human

SwissProt: Q3UZ39 Mouse SwissProt: Q66HF9 Rat

Unigene: 471779 Human

Unigene: 395990 Mouse

Unigene: 45039 Mouse

Unigene: 153995 Rat

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.