BIOTECHNOLOGY BIOENGINEERING
Volume 104, Number 6, December 15, 2009
FEATURES
v Spotlight
Editors’ Choice
1047 Cell-Free Production of Transducible Transcription Factors for Nuclear Reprogramming W.C. Yang, K.G. Patel, J. Lee, Y.T. Ghebremariam, H.E. Wong, J.P. Cooke, and J.R. Swartz page 1197
ARTICLES
Biocatalysis, Protein Engineering, and Nanobiotechnology
1059 Quantitative Characterization of Quantum Dot-Labeled Lambda Phage for Escherichia coli Detection P.B. Yim, M.L. Clarke, M. McKinstry, S.H. De Paoli Lacerda, L.F. Pease III, M.A. Dobrovolskaia, H. Kang, T.D. Read, S. Sozhamannan, and J. Hwang Published online 24 July 2009
page 1215 1068 Kinetic Limitations of a Bioelectrochemical Electrode Using Carbon Nanotube-Attached Glucose Oxidase for Biofuel Cells X. Zhao, H. Jia, J. Kim, and P. Wang Published online 7 August 2009
1075 Evaluation of Glucose Sensitive Affi nity Binding Assay Entrapped in Fluorescent Dissolved-Core Alginate Microspheres A. Chaudhary, M. Raina, H. Harma, P. Hanninen, M.J. McShane, and R. Srivastava Published online 4 August 2009
Biofuels and Environmental Biotechnology
page 1207 1086 Computational Framework for Predictive Biodegradation S.D. Finley, L.J. Broadbelt, and V. Hatzimanikatis Published online 31 July 2009
BBIT-104(6)-CNT.inddIT-104(6)-CNT.indd 1 110/30/090/30/09 4:53:164:53:16 PMPM 1098 Enhanced Hydrogen and 1,3-Propanediol Production From Glycerol by Fermentation Using Mixed Cultures P.A. Selembo, J.M. Perez, W.A. Lloyd, and B.E. Logan Published online 21 July 2009
Bioprocess Engineering and Supporting Technologies
1107 A Predictive High-Throughput Scale-Down Model of Monoclonal Antibody Production in CHO Cells R. Legmann, H.B. Schreyer, R.G. Combs, E.L. McCormick, A.P. Russo, and S.T. Rodgers page 1107 Published online 21 July 2009
1121 Selection and Mass Spectrometry Characterization of Peptides Targeting Semiconductor Surfaces E. Estephan, C. Larroque, N. Bec, P. Martineau, F.J.G. Cuisinier, T. Cloitre, and C. Gergely Published online 24 July 2009
Bioseparations and Downstream Processing
1132 Degradation of an Fc-Fusion Recombinant Protein by Host Cell Proteases: Identifi cation of a CHO Cathepsin D Protease F. Robert, H. Bierau, M. Rossi, D. Agugiaro, T. Soranzo, H. Broly, and C. Metchell-Logean Published online 4 August 2009
Cellular and Metabolic Engineering
1142 Visualizing and Quantifying the Differential Cleavages of the Eukaryotic Translation Initiation Factors eIF4GI and eIF4GII in the Enterovirus- Infected Cell Y.-Y. Hsu, Y.-N. Liu, W.-W. Lu, and S.-H. Kung Published online 4 August 2009 page 1068 1153 Fast Dynamic Response of the Fermentative Metabolism of Escherichia coli to Aerobic and Anaerobic Glucose Pulses A.R. Lara, H. Taymaz-Nikerel, M.R. Mashego, W.M. van Gulik, J.J. Heijnen, O.T. Ramírez, and W.A. van Winden Published online 14 August 2009
Engineering Science of Biological Systems
1162 Micronization of Lysozyme by Supercritical Assisted Atomization R. Adami, L.S. Osséo, and E. Reverchon Published online 14 July 2009
1171 Retroviral Vector Production Under Serum Deprivation: The Role of Lipids A.F. Rodrigues, M. Carmo, P.M. Alves, and A.S. Coroadinha Published online 4 August 2009
1182 Directed Attachment of Antibodies to Kinesin-Powered Molecular Shuttles A. Carroll-Portillo, M. Bachand, and G.D. Bachand Published online 14 August 2009
BBIT-104(6)-CNT.inddIT-104(6)-CNT.indd 2 110/30/090/30/09 4:53:274:53:27 PMPM Synthetic Biology
1189 Engineering Multigene Expression In Vitro and In Vivo With Small Terminators for T7 RNA Polymerase L. Du, R. Gao, and A.C. Forster Published online 31 July 2009
Tissue Engineering and Delivery Systems
1197 Transmural Flow Bioreactor for Vascular Tissue Engineering J.W. Bjork and R.T. Tranquillo Published online 14 July 2009 page 1075
1207 Controlled Release of Neurotrophin-3 From Fibrin-Based Tissue Engineering Scaffolds Enhances Neural Fiber Sprouting Following Subacute Spinal Cord Injury P.J. Johnson, S.R. Parker, and S.E. Sakiyama-Elbert Published online 14 July 2009
1215 Design and Validation of a Pulsatile Perfusion Bioreactor for 3D High Cell Density Cultures J.A. Chouinard, S. Gagnon, M.G. Couture, A. Lévesque, and P. Vermette Published online 14 July 2009
1224 Tracking Large Solid Constructs Suspended in a Rotating Bioreactor: A Combined Experimental and Theoretical Study L.J. Cummings, N.B.E. Sawyer, S.P. Morgan, F.R.A.J. Rose, and S.L. Waters Published online 21 August 2009
page 1182 COMMUNICATIONS TO THE EDITOR
Biocatalysis, Protein Engineering, and Nanobiotechnology
1235 Enzyme-Assisted Physicochemical Enantioseparation Processes - Part III: Overcoming Yield Limitations by Dynamic Kinetic Resolution of Aspara- gine Via Preferential Crystallization and Enzymatic Racemization K. Würges, K. Petruševska-Seebach, M.P. Elsner, and S. Lütz Published online 4 August 2009
Bioprocess Engineering and Supporting Technologies
1240 Microbioreactor Array for Full-Factorial Analysis of Provision of Multiple Soluble Factors in Cellular Microenvironments D. Titmarsh and J. Cooper-White Published online 14 August 2009
1246 Author Index
1254 Subject Index
BBIT-104(6)-CNT.inddIT-104(6)-CNT.indd 3 110/30/090/30/09 4:53:294:53:29 PMPM Cover Legend A new chromatography-free method promises to reduce recombinant peptide cost. Shown is the enzymatic cleavage of fusion protein, releasing a fi bril- forming peptide which is recovered by selective precipitation. See related article by Middelberg et al., Biotechnology and Bioengineering, Volume 104, Number 5 (December 1, 2009), pages 973–985. (Image courtesy of Dr. Yap Pang Chuan)
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