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Biotechnology Bioengineering BIOTECHNOLOGY BIOENGINEERING Volume 104, Number 6, December 15, 2009 FEATURES v Spotlight Editors’ Choice 1047 Cell-Free Production of Transducible Transcription Factors for Nuclear Reprogramming W.C. Yang, K.G. Patel, J. Lee, Y.T. Ghebremariam, H.E. Wong, J.P. Cooke, and J.R. Swartz page 1197 ARTICLES Biocatalysis, Protein Engineering, and Nanobiotechnology 1059 Quantitative Characterization of Quantum Dot-Labeled Lambda Phage for Escherichia coli Detection P.B. Yim, M.L. Clarke, M. McKinstry, S.H. De Paoli Lacerda, L.F. Pease III, M.A. Dobrovolskaia, H. Kang, T.D. Read, S. Sozhamannan, and J. Hwang Published online 24 July 2009 page 1215 1068 Kinetic Limitations of a Bioelectrochemical Electrode Using Carbon Nanotube-Attached Glucose Oxidase for Biofuel Cells X. Zhao, H. Jia, J. Kim, and P. Wang Published online 7 August 2009 1075 Evaluation of Glucose Sensitive Affi nity Binding Assay Entrapped in Fluorescent Dissolved-Core Alginate Microspheres A. Chaudhary, M. Raina, H. Harma, P. Hanninen, M.J. McShane, and R. Srivastava Published online 4 August 2009 Biofuels and Environmental Biotechnology page 1207 1086 Computational Framework for Predictive Biodegradation S.D. Finley, L.J. Broadbelt, and V. Hatzimanikatis Published online 31 July 2009 BBIT-104(6)-CNT.inddIT-104(6)-CNT.indd 1 110/30/090/30/09 4:53:164:53:16 PPMM 1098 Enhanced Hydrogen and 1,3-Propanediol Production From Glycerol by Fermentation Using Mixed Cultures P.A. Selembo, J.M. Perez, W.A. Lloyd, and B.E. Logan Published online 21 July 2009 Bioprocess Engineering and Supporting Technologies 1107 A Predictive High-Throughput Scale-Down Model of Monoclonal Antibody Production in CHO Cells R. Legmann, H.B. Schreyer, R.G. Combs, E.L. McCormick, A.P. Russo, and S.T. Rodgers page 1107 Published online 21 July 2009 1121 Selection and Mass Spectrometry Characterization of Peptides Targeting Semiconductor Surfaces E. Estephan, C. Larroque, N. Bec, P. Martineau, F.J.G. Cuisinier, T. Cloitre, and C. Gergely Published online 24 July 2009 Bioseparations and Downstream Processing 1132 Degradation of an Fc-Fusion Recombinant Protein by Host Cell Proteases: Identifi cation of a CHO Cathepsin D Protease F. Robert, H. Bierau, M. Rossi, D. Agugiaro, T. Soranzo, H. Broly, and C. Metchell-Logean Published online 4 August 2009 Cellular and Metabolic Engineering 1142 Visualizing and Quantifying the Differential Cleavages of the Eukaryotic Translation Initiation Factors eIF4GI and eIF4GII in the Enterovirus- Infected Cell Y.-Y. Hsu, Y.-N. Liu, W.-W. Lu, and S.-H. Kung Published online 4 August 2009 page 1068 1153 Fast Dynamic Response of the Fermentative Metabolism of Escherichia coli to Aerobic and Anaerobic Glucose Pulses A.R. Lara, H. Taymaz-Nikerel, M.R. Mashego, W.M. van Gulik, J.J. Heijnen, O.T. Ramírez, and W.A. van Winden Published online 14 August 2009 Engineering Science of Biological Systems 1162 Micronization of Lysozyme by Supercritical Assisted Atomization R. Adami, L.S. Osséo, and E. Reverchon Published online 14 July 2009 1171 Retroviral Vector Production Under Serum Deprivation: The Role of Lipids A.F. Rodrigues, M. Carmo, P.M. Alves, and A.S. Coroadinha Published online 4 August 2009 1182 Directed Attachment of Antibodies to Kinesin-Powered Molecular Shuttles A. Carroll-Portillo, M. Bachand, and G.D. Bachand Published online 14 August 2009 BBIT-104(6)-CNT.inddIT-104(6)-CNT.indd 2 110/30/090/30/09 4:53:274:53:27 PPMM Synthetic Biology 1189 Engineering Multigene Expression In Vitro and In Vivo With Small Terminators for T7 RNA Polymerase L. Du, R. Gao, and A.C. Forster Published online 31 July 2009 Tissue Engineering and Delivery Systems 1197 Transmural Flow Bioreactor for Vascular Tissue Engineering J.W. Bjork and R.T. Tranquillo Published online 14 July 2009 page 1075 1207 Controlled Release of Neurotrophin-3 From Fibrin-Based Tissue Engineering Scaffolds Enhances Neural Fiber Sprouting Following Subacute Spinal Cord Injury P.J. Johnson, S.R. Parker, and S.E. Sakiyama-Elbert Published online 14 July 2009 1215 Design and Validation of a Pulsatile Perfusion Bioreactor for 3D High Cell Density Cultures J.A. Chouinard, S. Gagnon, M.G. Couture, A. Lévesque, and P. Vermette Published online 14 July 2009 1224 Tracking Large Solid Constructs Suspended in a Rotating Bioreactor: A Combined Experimental and Theoretical Study L.J. Cummings, N.B.E. Sawyer, S.P. Morgan, F.R.A.J. Rose, and S.L. Waters Published online 21 August 2009 page 1182 COMMUNICATIONS TO THE EDITOR Biocatalysis, Protein Engineering, and Nanobiotechnology 1235 Enzyme-Assisted Physicochemical Enantioseparation Processes - Part III: Overcoming Yield Limitations by Dynamic Kinetic Resolution of Aspara- gine Via Preferential Crystallization and Enzymatic Racemization K. Würges, K. Petruševska-Seebach, M.P. Elsner, and S. Lütz Published online 4 August 2009 Bioprocess Engineering and Supporting Technologies 1240 Microbioreactor Array for Full-Factorial Analysis of Provision of Multiple Soluble Factors in Cellular Microenvironments D. Titmarsh and J. Cooper-White Published online 14 August 2009 1246 Author Index 1254 Subject Index BBIT-104(6)-CNT.inddIT-104(6)-CNT.indd 3 110/30/090/30/09 4:53:294:53:29 PPMM Cover Legend A new chromatography-free method promises to reduce recombinant peptide cost. Shown is the enzymatic cleavage of fusion protein, releasing a fi bril- forming peptide which is recovered by selective precipitation. See related article by Middelberg et al., Biotechnology and Bioengineering, Volume 104, Number 5 (December 1, 2009), pages 973–985. (Image courtesy of Dr. Yap Pang Chuan) Biotechnology and Bioengineering (Print ISSN: 0006-3592; Online ISSN: 1097-0290), is published monthly, and semi-monthly in February, April, June, August, October, and December, by Wiley Subscription Services, Inc., a Wiley Company, 111 River St., Hoboken, NJ 07030-5774. Periodical Postage Paid at Hoboken, NJ and additional offi ces. Postmaster: Send all address changes to Biotechnology and Bioengineering, Journal Customer Services, John Wiley & Sons, Inc., 350 Main St., Malden, MA 02148-5020. Information for Subscribers: Biotechnology and Bioengineering is published in 18 issues per year. 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