April 15, 2014 u vol. 111 u no. 15 u 5445–5754

Cover image: Pictured is a section of an ovarian follicle from a woman with polycystic ovarian syndrome (PCOS). Brown immunohistochemical staining reveals the presence of the DENND1A protein in the theca cells of the follicle, where excess androgens are produced in PCOS ovaries. Jan M. McAllister et al. identified increased expression of DENND1A variant proteins in the cytoplasm and nucleus of PCOS theca and granulosa cells. Overexpression of the DENND1A variant 2 (DENND1A.V2) protein in normal theca cells caused a PCOS phenotype of in- creased androgen production, whereas knock-down of DENND1A.V2 in PCOS theca cells decreased androgen synthesis. The authors suggest that the PCOS candidate gene DENND1A might serve as a key regulator of androgen production in the disorder. See the article by McAllisteretal.onpagesE1519–E1527. Image courtesy of Jan M. McAllister. From the Cover

E1519 Protein expression in polycystic ovarian syndrome E1510 Oil spill impacts on developing fish hearts 5604 Chemical diversity in Amazonian canopy trees 5610 Thermal limits of ectotherms 5634 Geothermal refuges for life during glacial cycles

E1453 Reply to Corbeil et al.: Dedifferentiation Contents and multipotency Tetsuro Kusaba and Benjamin D. Humphreys

— THIS WEEK IN PNAS SCIENCE AND CULTURE How science intersects with culture

5445 In This Issue 5447 Supersized viruses Stephen Ornes

LETTERS (ONLINE ONLY) COMMENTARIES

E1447 Mangrove reemergence in the northernmost range 5448 Vivid watercolor paintbox for eukaryotic algae limit of eastern Florida Katrina T. Forest Chandra P. Giri and Jordan Long See companion article on page 3871 of issue 10 in volume 111 E1449 Reply to Giri and Long: Freeze-mediated expansion 5450 Biologically grounded predictions of species resistance of mangroves does not depend on whether expansion and resilience to climate change is emergence or reemergence Joseph Bernardo Kyle C. Cavanaugh, James R. Kellner, Alexander J. Forde, See companion article on page 5610 Daniel S. Gruner, John D. Parker, Wilfrid Rodriguez, and Ilka C. Feller 5452 Diverse cryptic refuges for life during glaciation Stephen B. Pointing, Barbara Bollard-Breen, E1450 Strong evidence for protein epistasis, weak evidence and Lennard N. Gillman against it See companion article on page 5634 David D. Pollock and Richard A. Goldstein E1451 CD133 might be a pan marker of epithelial cells with dedifferentiation capacity PNAS PLUS Denis Corbeil, Christine A. Fargeas, and József Jászai 5454 Significance Statements Brief statements written by the authors about the significance of Free online through the PNAS open access option. their papers.

PNAS u April 15, 2014 u vol. 111 u no. 15 u iii–vii Downloaded by guest on October 1, 2021 5598 Fine-scale ecological and economic assessment of PHYSICAL SCIENCES climate change on olive in the Mediterranean Basin reveals winners and losers CHEMISTRY Luigi Ponti, Andrew Paul Gutierrez, Paolo Michele Ruti, and Alessandro Dell’Aquila 5456 Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes Peyton Shieh, M. Sloan Siegrist, Andrew J. Cullen, PSYCHOLOGICAL AND COGNITIVE SCIENCES and Carolyn R. Bertozzi 5503 Oxytocin promotes group-serving dishonesty 5532 Understanding the mechanism of proteasome 20S core Shaul Shalvi and Carsten K. W. De Dreu particle gating Michael P. Latham, Ashok Sekhar, and Lewis E. Kay BIOLOGICAL SCIENCES COMPUTER SCIENCES E1454 Compound facial expressions of emotion APPLIED BIOLOGICAL SCIENCES Shichuan Du, Yong Tao, and Aleix M. Martinez 5508 Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo EARTH, ATMOSPHERIC, AND PLANETARY SCIENCES Mark Juhas, George C. Engelmayr, Jr., Andrew N. 5462 Methanogenic burst in the end-Permian carbon cycle Fontanella, Gregory M. Palmer, and Nenad Bursac Daniel H. Rothman, Gregory P. Fournier, Katherine L. French, Eric J. Alm, Edward A. Boyle, Changqun Cao, and Roger E. Summons BIOCHEMISTRY 5456 Imaging bacterial peptidoglycan with near-infrared 5468 SQUID–SIMS is a useful approach to uncover primary fluorogenic azide probes signals in the Archean sulfur cycle Peyton Shieh, M. Sloan Siegrist, Andrew J. Cullen, Woodward W. Fischer, David A. Fike, Jena E. Johnson, and Carolyn R. Bertozzi Timothy D. Raub, Yunbin Guan, Joseph L. Kirschvink, and John M. Eiler 5514 The docking protein FRS2α is a critical regulator of VEGF receptors signaling 5474 The origin of methanethiol in midocean ridge Pei-Yu Chen, Lingfeng Qin, Zhen W. Zhuang, George hydrothermal fluids Tellides, Irit Lax, Joseph Schlessinger, and Michael Simons Eoghan P. Reeves, Jill M. McDermott, and Jeffrey S. Seewald 5520 Dynamic regulation of FGF23 by Fam20C 5480 Radiocarbon evidence for alternating northern and phosphorylation, GalNAc-T3 glycosylation, southern sources of ventilation of the deep Atlantic and furin proteolysis carbon pool during the last deglaciation Vincent S. Tagliabracci, James L. Engel, Sandra E. Luke C. Skinner, Claire Waelbroeck, Adam E. Scrivner, Wiley, Junyu Xiao, David J. Gonzalez, Hitesh and Stewart J. Fallon Nidumanda Appaiah, Antonius Koller, Victor Nizet, Kenneth E. White, and Jack E. Dixon ENGINEERING 5526 Chronology in lesion tolerance gives priority to 5700 Cellular mechanisms for integral feedback in visually genetic variability guided behavior Karel Naiman, Gaëlle Philippin, Robert P. Fuchs, Bettina Schnell, Peter T. Weir, Eatai Roth, Adrienne L. and Vincent Pagès Fairhall, and Michael H. Dickinson

BIOPHYSICS AND COMPUTATIONAL BIOLOGY MATHEMATICS E1463 Molecular mechanism for differential recognition 5485 Higher-order dangers and precisely constructed taxa of membrane phosphatidylserine by the immune in models of randomness regulatory receptor Tim4 Steve Pincus and Burton H. Singer Gregory T. Tietjen, Zhiliang Gong, Chiu-Hao Chen, Ernesto Vargas, James E. Crooks, Kathleen D. Cao, PHYSICS Charles T. R. Heffern, J. Michael Henderson, Mati 5491 Observing (non)linear lattice dynamics in graphite by Meron, Binhua Lin, Benoît Roux, Mark L. Schlossman, ultrafast Kikuchi diffraction Theodore L. Steck, Ka Yee C. Lee, and Erin J. Adams Wenxi Liang, Giovanni M. Vanacore, and Ahmed H. Zewail E1473 From a structural average to the conformational ensemble of a DNA bulge Xuesong Shi, Kyle A. Beauchamp, Pehr B. Harbury, SOCIAL SCIENCES and Daniel Herschlag 5532 Understanding the mechanism of proteasome 20S core ECONOMIC SCIENCES particle gating Michael P. Latham, Ashok Sekhar, and Lewis E. Kay 5497 Preparedness of Americans for the Affordable Care Act Silvia Helena Barcellos, Amelie C. Wuppermann, 5538 A frameshifting stimulatory stem loop destabilizes the Katherine Grace Carman, Sebastian Bauhoff, Daniel L. hybrid state and impedes ribosomal translocation McFadden, Arie Kapteyn, Joachim K. Winter, Hee-Kyung Kim, Fei Liu, Jingyi Fei, Carlos Bustamante, and Dana Goldman Ruben L. Gonzalez, Jr., and Ignacio Tinoco, Jr.

iv u www.pnas.org Downloaded by guest on October 1, 2021 5544 Deep classification of a large cryo-EM dataset defines ECOLOGY the conformational landscape of the 26S proteasome 5598 Fine-scale ecological and economic assessment of ł   Pia Unverdorben, Florian Beck, Pawe Sledz, Andreas climate change on olive in the Mediterranean Schweitzer, Günter Pfeifer, Jürgen M. Plitzko, Basin reveals winners and losers Wolfgang Baumeister, and Friedrich Förster Luigi Ponti, Andrew Paul Gutierrez, Paolo Michele Ruti, ’ 5550 Crosstalk and the evolution of specificity in and Alessandro Dell Aquila two-component signaling 5604 Amazonian functional diversity from forest canopy Michael A. Rowland and Eric J. Deeds chemical assembly 5556 Solid-state electron transport via cytochrome c depends Gregory P. Asner, Roberta E. Martin, Raul Tupayachi, on electronic coupling to electrodes and across Christopher B. Anderson, Felipe Sinca, Loreli the protein Carranza-Jiménez, and Paola Martinez Nadav Amdursky, Doron Ferber, Carlo Augusto Bortolotti, 5610 Thermal-safety margins and the necessity of Dmitry A. Dolgikh, Rita V. Chertkova, Israel Pecht, thermoregulatory behavior across latitude Mordechai Sheves, and David Cahen and elevation 5562 Dimerization of mammalian kinesin-3 motors results Jennifer M. Sunday, Amanda E. Bates, Michael R. in superprocessive motion Kearney, Robert K. Colwell, Nicholas K. Dulvy, Virupakshi Soppina, Stephen R. Norris, Aslan S. Dizaji, John T. Longino, and Raymond B. Huey Matt Kortus, Sarah Veatch, Michelle Peckham, See Commentary on page 5450 and Kristen J. Verhey 5616 Trait-mediated assembly processes predict successional 5568 Force-dependent isomerization kinetics of a highly changes in community diversity of tropical forests conserved proline switch modulates the Jesse R. Lasky, María Uriarte, Vanessa K. Boukili, mechanosensing region of filamin and Robin L. Chazdon Ž Lorenz Rognoni, Tobias Möst, Gabriel oldák, 5622 Competition–dispersal tradeoff ecologically and Matthias Rief differentiates recently speciated marine bacterioplankton populations CELL BIOLOGY Yutaka Yawata, Otto X. Cordero, Filippo Menolascina, Jan-Hendrik Hehemann, Martin F. Polz, and Roman Stocker E1481 Protein aggregation can inhibit clathrin-mediated endocytosis by chaperone competition 5628 Deoxygenation of the Baltic Sea during the last century Anan Yu, Yoko Shibata, Bijal Shah, Barbara Calamini, Jacob Carstensen, Jesper H. Andersen, Bo G. Gustafsson, Donald C. Lo, and Richard I. Morimoto and Daniel J. Conley 5574 Sumoylation differentially regulates Sp1 to control cell differentiation ENVIRONMENTAL SCIENCES Lili Gong, Wei-Ke Ji, Xiao-Hui Hu, Wen-Feng Hu, E1510 Deepwater Horizon crude oil impacts the developing Xiang-Cheng Tang, Zhao-Xia Huang, Ling Li, hearts of large predatory pelagic fish Mugen Liu, Shi-Hua Xiang, Erxi Wu, Zachary John P. Incardona, Luke D. Gardner, Tiffany L. Linbo, Woodward, Yi-Zhi Liu, Quan Dong Nguyen, Tanya L. Brown, Andrew J. Esbaugh, Edward M. Mager, and David Wan-Cheng Li John D. Stieglitz, Barbara L. French, Jana S. Labenia, Cathy A. Laetz, Mark Tagal, Catherine A. Sloan, 5580 Combined hydrogels that switch human pluripotent Abigail Elizur, Daniel D. Benetti, Martin Grosell, stem cells from self-renewal to differentiation Barbara A. Block, and Nathaniel L. Scholz James E. Dixon, Disheet A. Shah, Catherine Rogers, Stephen Hall, Nicola Weston, Christopher D. J. EVOLUTION Parmenter, Donal McNally, Chris Denning, and Kevin M. Shakesheff 5462 Methanogenic burst in the end-Permian carbon cycle Daniel H. Rothman, Gregory P. Fournier, Katherine L. French, Eric J. Alm, Edward A. Boyle, Changqun Cao, DEVELOPMENTAL BIOLOGY and Roger E. Summons E1491 Acentriolar mitosis activates a p53-dependent – apoptosis pathway in the mouse embryo 5468 SQUID SIMS is a useful approach to uncover primary Hisham Bazzi and Kathryn V. Anderson signals in the Archean sulfur cycle Woodward W. Fischer, David A. Fike, Jena E. Johnson, E1501 Epidermal Wnt/β-catenin signaling regulates adipocyte Timothy D. Raub, Yunbin Guan, Joseph L. Kirschvink, differentiation via secretion of adipogenic factors and John M. Eiler Giacomo Donati, Valentina Proserpio, Beate Maria Lichtenberger, Ken Natsuga, Rodney Sinclair, 5634 Geothermal activity helps life survive glacial cycles Hironobu Fujiwara, and Fiona M. Watt Ceridwen I. Fraser, Aleks Terauds, John Smellie, Peter Convey, and Steven L. Chown 5586 Spatial constraints control cell proliferation in tissues See Commentary on page 5452 Sebastian J. Streichan, Christian R. Hoerner, Tatjana Schneidt, Daniela Holzer, and Lars Hufnagel GENETICS 5592 O-GlcNAc reports ambient temperature and confers 5640 Bypass of the pre-60S ribosomal quality control as heat resistance on ectotherm development a pathway to oncogenesis Pablo T. Radermacher, Faina Myachina, Fritz Bosshardt, Sergey O. Sulima, Stephanie Patchett, Vivek M. Advani, Rahul Pandey, Daniel Mariappa, H.-Arno J. Müller, Kim De Keersmaecker, Arlen W. Johnson, and Christian F. Lehner and Jonathan D. Dinman

PNAS u April 15, 2014 u vol. 111 u no. 15 u v Downloaded by guest on October 1, 2021 IMMUNOLOGY 5688 Angucyclines as signals modulate the behaviors of 5646 Pivotal role of RNA-binding E3 ubiquitin ligase MEX3C Streptomyces coelicolor in RIG-I–mediated antiviral innate immunity Weishan Wang, Junjie Ji, Xiao Li, Juan Wang, Shanshan Li, Kanako Kuniyoshi, Osamu Takeuchi, Surya Pandey, Takashi Guohui Pan, Keqiang Fan, and Keqian Yang Satoh, Hidenori Iwasaki, Shizuo Akira, and Taro Kawai 5694 Global dissemination of a multidrug resistant Escherichia 5652 Receptor revision in CD4 T cells is influenced by follicular coli clone helper T cell formation and germinal-center interactions Nicola K. Petty, Nouri L. Ben Zakour, Mitchell Stanton-Cook, Lauren E. Higdon, Katherine A. Deets, Travis J. Friesen, Elizabeth Skippington, Makrina Totsika, Brian M. Kai-Yin Sze, and Pamela J. Fink Forde, Minh-Duy Phan, Danilo Gomes Moriel, Kate M. Peters, Mark Davies, Benjamin A. Rogers, 5658 Enforcement of γδ-lineage commitment by the Gordon Dougan, Jesús Rodriguez-Baño, Alvaro pre–T-cell receptor in precursors with weak Pascual, Johann D. D. Pitout, Mathew Upton, David L. γδ-TCR signals Paterson,TimothyR.Walsh,MarkA.Schembri, Payam Zarin, Gladys W. Wong, Mahmood Mohtashami, and Scott A. Beatson David L. Wiest, and Juan Carlos Zúñiga-Pflücker

5664 T helper 17 cells play a critical pathogenic role in NEUROSCIENCE lung cancer Seon Hee Chang, Seyedeh Golsar Mirabolfathinejad, 5700 Cellular mechanisms for integral feedback in visually Harshadadevi Katta, Amber M. Cumpian, Lei Gong, guided behavior Mauricio S. Caetano, Seyed Javad Moghaddam, Bettina Schnell, Peter T. Weir, Eatai Roth, Adrienne L. and Chen Dong Fairhall, and Michael H. Dickinson 5670 Cryptic MHC class I-binding peptides are revealed 5706 Differential ubiquitination and degradation of by aminoglycoside-induced stop codon read-through huntingtin fragments modulated by ubiquitin-protein into the 3′ UTR ligase E3A Elliot Goodenough, Tara M. Robinson, Matthew B. Zook, Kavita P. Bhat, Sen Yan, Chuan-En Wang, Shihua Li, Kevin M. Flanigan, John F. Atkins, Michael T. Howard, and Xiao-Jiang Li and Laurence C. Eisenlohr 5712 Functional evolution of Erg potassium channel gating

reveals an ancient origin for IKr MEDICAL SCIENCES Alexandra S. Martinson, Damian B. van Rossum, E1519 Overexpression of a DENND1A isoform produces Fortunay H. Diatta, Michael J. Layden, Sarah A. a polycystic ovary syndrome theca phenotype Rhodes, Mark Q. Martindale, and Timothy Jegla Jan M. McAllister, Bhavi Modi, Bruce A. Miller, 5718 Neural representations of ethologically relevant Jessica Biegler, Richard Bruggeman, Richard S. Legro, hand/mouth synergies in the human precentral gyrus and Jerome F. Strauss III Michel Desmurget, Nathalie Richard, Sylvain Harquel, 5676 IL-15 adjuvanted multivalent vaccinia-based universal Pierre Baraduc, Alexandru Szathmari, Carmine Mottolese, + influenza vaccine requires CD4 T cells for and Angela Sirigu heterosubtypic protection Sophie A. Valkenburg, Olive T. W. Li, Polly W. Y. Mak, Chris K. P. Mok, John M. Nicholls, Yi Guan, Thomas A. PHYSIOLOGY Waldmann, J. S. Malik Peiris, Liyanage P. Perera, E1454 Compound facial expressions of emotion and Leo L. M. Poon Shichuan Du, Yong Tao, and Aleix M. Martinez

5682 Bcl2L13 is a ceramide synthase inhibitor in glioblastoma 5723 Microtubule binding distinguishes dystrophin Samuel A. Jensen, Andrea E. Calvert, Giora Volpert, from utrophin Fotini M. Kouri, Lisa A. Hurley, Janina P. Luciano, Joseph J. Belanto, Tara L. Mader, Michael D. Eckhoff, Yongfei Wu, Alexandra Chalastanis, Anthony H. Dana M. Strandjord, Glen B. Banks, Melissa K. Futerman, and Alexander H. Stegh Gardner, Dawn A. Lowe, and James M. Ervasti

MICROBIOLOGY 5729 Systemic Activin signaling independently regulates sugar homeostasis, cellular metabolism, and pH balance E1528 Heat shock protein 90 controls HIV-1 reactivation in Drosophila melanogaster from latency Arpan C. Ghosh and Michael B. O’Connor Ian Anderson, Jun Siong Low, Stuart Weston, Michael Weinberger, Alexander Zhyvoloup, Aksana A. Labokha, 5735 Redirecting intracellular trafficking and the secretion Gianmarco Corazza, Russell A. Kitson, Christopher J. pattern of FSH dramatically enhances ovarian Moody, Alessandro Marcello, and Ariberto Fassati function in mice Huizhen Wang, Melissa Larson, Albina Jablonka-Shariff, E1538 Uncoupling reproduction from metabolism extends Christopher A. Pearl, William L. Miller, P. Michael Conn, chronological lifespan in yeast Irving Boime, and T. Rajendra Kumar Saisubramanian Nagarajan, Arthur L. Kruckeberg, Karen H. Schmidt, Evgueny Kroll, Morgan Hamilton, Kate McInnerney, Ryan Summers, Timothy Taylor, PLANT BIOLOGY and Frank Rosenzweig 5741 Abscisic acid sensor RCAR7/PYL13, specific regulator E1548 Proteomic analysis of Vibrio cholerae outer of protein phosphatase coreceptors membrane vesicles Stefan Fuchs, Stefanie V. Tischer, Christian Wunschel, Emrah Altindis, Yang Fu, and John J. Mekalanos Alexander Christmann, and Erwin Grill

vi u www.pnas.org Downloaded by guest on October 1, 2021 SYSTEMS BIOLOGY PHYSICS 5747 Identifying regulatory mechanisms underlying 5753 Testing Turing’s theory of morphogenesis in tumorigenesis using locus expression signature analysis chemical cells Eunjee Lee, Jeroen de Ridder, Jaap Kool, Lodewyk F. A. Nathan Tompkins, Ning Li, Camille Girabawe, Wessels, and Harmen J. Bussemaker Michael Heymann, G. Bard Ermentrout, Irving R. Epstein, and Seth Fraden

CORRECTIONS BIOCHEMISTRY 5753 Overexpressing mouse model demonstrates the protective role of Muc5ac in the lungs ENGINEERING Camille Ehre, Erin N. Worthington, Rachael M. Liesman, 5753 Lipopeptide nanoparticles for potent and selective siRNA Barbara R. Grubb, Diane Barbier, Wanda K. O’Neal, delivery in rodents and nonhuman primates Jean-Michel Sallenave, Raymond J. Pickles, Yizhou Dong, Kevin T. Love, J. Robert Dorkin, Sasilada and Richard C. Boucher Sirirungruang, Yunlong Zhang, Delai Chen, Roman L. Bogorad, Hao Yin, Yi Chen, Arturo J. Vegas, Christopher A. MEDICAL SCIENCES Alabi, Gaurav Sahay, Karsten T. Olejnik, Weiheng Wang, Avi Schroeder, Abigail K. R. Lytton-Jean, Daniel J. 5754 Differentiated kidney epithelial cells repair injured Siegwart, Akin Akinc, Carmen Barnes, Scott A. Barros, proximal tubule Mary Carioto, Kevin Fitzgerald, Julia Hettinger, Varun Tetsuro Kusaba, Matthew Lalli, Rafael Kramann, Kumar, Tatiana I. Novobrantseva, June Qin, William Akio Kobayashi, and Benjamin D. Humphreys Querbes, Victor Koteliansky, Robert Langer, and Daniel G. Anderson ix Subscription Form

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