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Bul. Dept. Pl. Res. No. 37 Contents 1. Keshab Raj Rajbhandari 1 National Herbarium (KATH) and Flora of Nepal 2. Keshab R. Rajbhandari, Ganga D. Bhatt and Rita Chhetri 19 Paspalidium geminatum (Forssk.) Stapf (Poaceae), a new record for Nepal Paspalidium ... (A Scientific Publication) 3. Mitra Lal Pathak and Dil Bahadur Chhetri 21 Senna alata (Linnaeus) Roxburgh (Leguminosae), a new addition to the Flora of Nepal... 4. Mitra Lal Pathak, Narahari Chapagain and Rajendra Acharya 23 Floristic composition of Parsa Wildlife Reserve, Central Nepal 5. M. K. Adhikari and S. Devkota 36 Addition and checklist to the Xylarial fungi from Nepal. 6. Krishna Ram Bhattarai and Sunil Kumar Acharya 41 Documentation of ethnobotanical knowledge of Tharu people on the utilization of plant ... 7. Ras Bihari Mahato and Bed Nidhi Sharma 51 Common ethnomedicinal plants of Dhanusha District, Nepal 8. Keshari Maiya Rajkarnikar and Sabari Rajbahak 63 In Vitro multiplication of Paulownia fortuneii (Seem.) Hemsl. through seed cultureIn Vitro... 9. Sabari Rajbahak, Kesari Maiya Rajkarnikar 67 Micropropagation of Ficus religiosa L. through seed culture 10. Gaurav Parmar1and Bijaya Pant 72 Comparative study of in vitro growth rates of intra-generic orchid species, viz., Coelogyne stricta... 11. Kamal Bahadur Nepali, Dipak Lamichhane and Dinesh Baral 77 Study in the growth rate of Lilium nepalense D. Don in different soil combinations 12. Parasmani Yadav, Jyoti Joshi and Shantam Jha 81 Extraction of crystal piperine from black pepper 13. Seerjana Maharjan, Govinda Prasad Gautam, Bhadrika Bhattarai and Anuradha Gyawali 84 Pharmacognostical and preliminary phytochemical study of Gaultheria fragrantissima Wall... 14. Parasmani Yadav and Jyoti Joshi 89 Phytochemical screening of ferns collected from Maipokhari Ilam, Eastern region... 15. Kamal Bahadur Nepali 92 Carbon stock assessment in Bajrabarahi religious forest of Lalitpur District 16. Dol Raj Luitel and Mitra Pathak 97 Population status and ecology of Gnetum montanum in Eastern Nepal Government of Nepal 17. Kalpana Sharma (Dhakal) 101 Genetic resources of wild edible root and tuber plants from four districts of Nepal Ministry of Forests and Soil Conservation 18. Seerjana Maharjan and Rose Shrestha 107 Comparative root anatomical study of four wild Asparagus species found in Nepal... Department of Plant Resources 19. Sangeeta Swar 113 Thapathali, Kathmandu, Nepal Pharmacognostical study of some of the crude drugs of Nepal 2015 20. Rose Shrestha 116 Community focused management of medicinal plants in Makawanpur district, Central Nepal ISSN 1995 - 8579 Bulletin of Department of Plant Resources No. 37 PLANT RESOURCES Government of Nepal Ministry of Forests and Soil Conservation Department of Plant Resources Thapathali, Kathmandu, Nepal 2015 Advisory Board Mr. Yam Bahadur Thapa Ms. Sushma Upadhyaya Mr. Sanjeev Kumar Rai Managing Editor Ms. Nirmala Phuyal Editorial Board Mr. Dipak Lamichchane Ms. Sudhita Basukala Ms. Usha Tandukar Mr. Pramesh Bahadur Lakhey No. of Copies: 500 Cover Photo: Meconopsis discigera Prain, Marghor, Humla, 3850m Photo by Dr. K. R. Rajbhandari Date of Publication: April, 2015 © All rights reserved Department of Plant Resources (DPR) Thapathali, Kathmandu, Nepal Tel: 977-1-4251160, 4251161, 4268246 E-mail: [email protected] Citation: Name of the author, year of publication. Title of the paper, Bul. Dept. Pl. Res. N. 37, N. of pages, Department of Plant Resources, Kathmandu, Nepal. ISSN: 1995-8579 Published By: Publicity and Documentation Section Mr. B.K. Khakurel Department of Plant Resources (DPR), Kathmandu,Dr. K.R.Nepal Bhattarai Ms. N. Joshi Reviewers: Dr. M.N. Subedi Ms. Sushma Upadhyaya Mr. Sanjeev Kumar Rai Dr. Krishna Ram Amatya Ms. Lajmina Joshi Ms. Nirmala Joshi Ms. Rose Shrestha Dr. Bharat Babu Shrestha The issue can be retrieved from http://www.dpr.gov.np Government of Nepal 4251139 4251159 MINISTRY OF FORESTS & SOIL CONSERVATION { 4251160 4251171 DEPARTMENT OF PLANT RESOURCES Fax No.: 4251141 GRAM: VANSAPATI, KATHMANDU Ref. No.: THAPATHALI, KATHMANDU FOREWORD For a small country, Nepal has a tremendous geographic and climatic diversity due to its unique location in central Himalayan range. This geographic and climatic diversity has engendered even greater and richer biodiversity which has been a subject of interest for many scientists and naturalists. The rich resource has been utilized only partially and is yet to be fully prospected, documented and harnessed. The Department of Plant Resources (DPR) is one of the few departments of Government of Nepal with research mandate. It works to explore, document and sustainably utilize the immensely rich plant diversity of Nepal with the ultimate aim of economic development through proper use of plant resource. Among the various plant related resources, medicinal and aromatic plants and other non-timber forest products (NTFPs) have been of particular research interest of DPR. However, certain limitations and hurdles were being observed in the functioning of this department which are being addressed in this fiscal year. The legislative void in the working mechanism of DPR is being addressed by drafting of Plant Resource Act, 2071. Further, collaboration with national and international organizations and research institutes such as Tribhuwan University, Sowa National University for joint research is likely to pave the way towards fruitful collaborative research. Emphasis is being given to the writing of Flora of Nepal which will project a tentative, if not complete, inventory of Nepalese plant resources. Department, as a part of its trade support activities for plant based products, has developed generic guidelines for Good Agricultural Practices (GAP) and Good Collection Practices (GCP) for medicinal and aromatic plants (MAPs) which is being as a national standard. Similarly, detection survey of pests and diseases in five highly traded MAPs have been carried out for preparation of pest database which will help in unbarred trade of crude and processed herbal products. Accreditation of two analytical laboratories under DPR, which is under process, will also help boost export of these products. Publication of the research work is of utmost significance for the dissemination and documentation of the research findings so that scientific community as well as general public can benefit from them. This publication encompasses the research papers in the field of plant resources conducted in Nepal. It also includes the scientific presentation of the research work conducted within DPR and its central and district level offices, and as such, reflects discrete image of the research and explorative activities occurring under this department. I hope that the published scientific papers will help in proper evaluation and sustainable utilization of plant resources at both national and worldwide levels. Finally, I would like to thank the contributors, members of editorial board, reviewers and staffs of publicity and documentation section for their unwavering effort in bringing out this publication. ……………………………. Yam Bahadur Thapa Director General April 2015 Editorial It is indeed our great pleasure to bring out this issue of the Bulletin of the Department “Bull. Dept. Pl. Rs. No. 37” as a Scientific Publication in continuation of the year long tradition of publishing the scientific work carried by our currently working scientific staff as well as our esteemed retired faculty. Altogether 20 articles under different categories like plant systematics, ethnobotany, biotechnology, floristic survey, anatomy, phytochemical and pharmacognostical study as well as description of some new species of plants to Nepal have been included. Similarly very informative article on the National repository of plants, National Herbarium (KATH) and the Flora of Nepal has also been incorporated in the present issue. We hope that the research findings will be of great help to researchers, students, planners and all concerned. The painstaking effort of our scientists to bring out the publication in this form is highly appreciable. Similarly the valuable comments and suggestions by expert peer reviewers have always helped us to improve the quality of the Bulletin. We always expand our efforts to timely optimize the quality and standard of our publication. Constructive suggestion from our valued readers is always welcome and we are committed towards improving our future issues. The editorial board is fully liable for any shortcomings incurred in this issue. National Herbarium (KATH) and Flora of Nepal Keshab Raj Rajbhandari Former Scientific Officer, National Herbarium, Department of Plant Resources, Godawari, Lalitpur, Nepal Present address: G. P. O. Box 9446, Kathmandu, Nepal [email protected] Abstract The National Herbarium of Nepal belonging to the Department of Plant Resources, Government of Nepal is meant for explorations of plants in Nepal, their collections, documentation of the herbarium specimens and publishing flora of Nepal. KATH is used as acronym of the National Herbarium of Nepal. This paper deals with the activities of the National Herbarium of Nepal for the publication of Flora of Nepal (flowering plants and ferns). Flora of Nepal now is an international activity and the National Herbarium plays an important role in it. At present there are more than 150,000 sheets of plant specimens housed in the National Herbarium and about 30,000 specimens are still to be identified and housed. There are 4443 species of flowering plants under 1403 genera and 203 families