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Expression of Recombinant Human Androgen Receptor and Its Use for Screening Methods
Institut für Physiologie FML Weihenstephan Technische Universität München Expression of recombinant human androgen receptor and its use for screening methods Ellinor Rose Sigrid Bauer Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften genehmigten Dissertation. Vorsitzender: Univ.-Prof. Dr. B. Hock Prüfer der Dissertation: Univ.-Prof. Dr. H. H. D. Meyer Univ.-Prof. Dr. H. Sauerwein (Rheinische Friedrich-Wilhelms-Universität Bonn) Die Dissertation wurde am 31.10.2002 bei der Technischen Universität München eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt am 03.12.2002 angenommen. Introduction Content 1. INTRODUCTION ..................................................................................................................................... 5 1.1. ENDOCRINE DISRUPTERS 5 1.2. ANDROGENS AND ANTIANDROGENS 7 1.2.1. DEFINITIONS 7 1.2.2. MODE OF ACTION 8 1.3. STRUCTURES OF ENDOCRINE DISRUPTERS 10 1.4. STRATEGIES FOR MONITORING ANDROGEN ACTIVE SUBSTANCES 13 1.4.1. IN VIVO METHODS 13 1.4.2. IN VITRO METHODS 15 1.5. OBJEKTIVE OF THE STUDIES 18 2. MATERIALS AND METHODS ................................................................................................................. 19 2.1. PREPARATION OF RECEPTORS 19 2.2. ASSAY SYSTEMS 19 2.2.1. IN SOLUTION AR ASSAY 19 2.2.2. IMMUNO-IMMOBILISED RECEPTOR ASSAY (IRA) 20 2.2.3. PR AND SHBG ASSAYS 21 2.2.4. DATA EVALUATION 21 2.3. ANALYTES 22 3. RESULTS AND DISCUSSION ................................................................................................................. 23 3.1. DEVELOPMENT OF NEW ASSAY SYSTEMS 23 3.1.1. BAR ASSAY 23 3.1.2. CLONING OF THE HUMAN AR AND PRODUCTION OF FUNCTIONAL PROTEIN 24 3.1.3. DEVELOPMENT OF A SCREENING ASSAY ON MICROTITRE PLATES (IRA) 25 3.2. -
5 Part 1300—Definitions
PART 1300—DEFINITIONS (7) 5-androstenediol (3b,17b-dihydroxy- androst-5-ene) Sec. (8) 1-androstenedione ([5a]-androst-1- 1300.01 Definitions relating to controlled en-3,17-dione) substances. (9) 4-androstenedione (androst-4-en- 1300.02 Definitions relating to listed chemi- 3,17-dione) cals. (10) 5-androstenedione (androst-5-en- 1300.03 Definitions relating to electronic or- 3,17-dione) ders for controlled substances and elec- tronic prescriptions for controlled sub- (11) bolasterone (7a,17a-dimethyl-17b- stances. hydroxyandrost-4-en-3-one) 1300.04 Definitions relating to the dis- (12) boldenone (17b-hydroxyandrost-1,4- pensing of controlled substances by diene-3-one) means of the Internet. (13) boldione (androsta-1,4-diene-3,17- 1300.05 Definitions relating to the disposal dione) of controlled substances. (14) calusterone (7b,17a-dimethyl-17b- AUTHORITY: 21 U.S.C. 802, 821, 822, 829, hydroxyandrost-4-en-3-one) 871(b), 951, 958(f). (15) clostebol (4-chloro-17b- SOURCE: 62 FR 13941, Mar. 24, 1997, unless hydroxyandrost-4-en-3-one) otherwise noted. (16) dehydrochloromethyltestosterone (4-chloro-17b-hydroxy-17a-methyl- § 1300.01 Definitions relating to con- androst-1,4-dien-3-one) trolled substances. (17) desoxymethyltestosterone (17a- (a) Any term not defined in this part methyl-5a-androst-2-en-17b-ol) shall have the definition set forth in (a.k.a. ‘madol‘) section 102 of the Act (21 U.S.C. 802), (18) D1-dihydrotestosterone (a.k.a.‘1- except that certain terms used in part testosterone‘) (17b-hydroxy-5a- 1316 of this chapter are defined at the androst-1-en-3-one) beginning of each subpart of that part. -
(Cyp19a1b-GFP) Zebrafish Embryos
Screening Estrogenic Activities of Chemicals or Mixtures In Vivo Using Transgenic (cyp19a1b-GFP) Zebrafish Embryos Franc¸ois Brion1, Yann Le Page2, Benjamin Piccini1, Olivier Cardoso1, Sok-Keng Tong3, Bon-chu Chung3, Olivier Kah2* 1 Unite´ d’Ecotoxicologie in vitro et in vivo, Direction des Risques Chroniques, Institut National de l’Environnement Industriel et des Risques (INERIS), Verneuil-en- Halatte, France, 2 Universite´ de Rennes 1, Institut de Recherche Sante´ Environnement & Travail (IRSET), INSERM U1085, BIOSIT, Campus de Beaulieu, Rennes France, 3 Taiwan Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan Abstract The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. This gene has two major characteristics: (i) it is only expressed in radial glial progenitors in the brain of fish and (ii) it is exquisitely sensitive to estrogens. Based on these properties, we demonstrate that natural or synthetic hormones (alone or in binary mixture), including androgens or progestagens, and industrial chemicals induce a concentration-dependent GFP expression in radial glial progenitors. As GFP expression can be quantified by in vivo imaging, this model presents a very powerful tool to screen and characterize compounds potentially acting as estrogen mimics either directly or after metabolization by the zebrafish embryo. This study also shows that radial glial cells that act as stem cells are direct targets for a large panel of endocrine disruptors, calling for more attention regarding the impact of environmental estrogens and/or certain pharmaceuticals on brain development. Altogether these data identify this in vivo bioassay as an interesting alternative to detect estrogen mimics in hazard and risk assessment perspective. -
Us Anti-Doping Agency
2019U.S. ANTI-DOPING AGENCY WALLET CARDEXAMPLES OF PROHIBITED AND PERMITTED SUBSTANCES AND METHODS Effective Jan. 1 – Dec. 31, 2019 CATEGORIES OF SUBSTANCES PROHIBITED AT ALL TIMES (IN AND OUT-OF-COMPETITION) • Non-Approved Substances: investigational drugs and pharmaceuticals with no approval by a governmental regulatory health authority for human therapeutic use. • Anabolic Agents: androstenediol, androstenedione, bolasterone, boldenone, clenbuterol, danazol, desoxymethyltestosterone (madol), dehydrochlormethyltestosterone (DHCMT), Prasterone (dehydroepiandrosterone, DHEA , Intrarosa) and its prohormones, drostanolone, epitestosterone, methasterone, methyl-1-testosterone, methyltestosterone (Covaryx, EEMT, Est Estrogens-methyltest DS, Methitest), nandrolone, oxandrolone, prostanozol, Selective Androgen Receptor Modulators (enobosarm, (ostarine, MK-2866), andarine, LGD-4033, RAD-140). stanozolol, testosterone and its metabolites or isomers (Androgel), THG, tibolone, trenbolone, zeranol, zilpaterol, and similar substances. • Beta-2 Agonists: All selective and non-selective beta-2 agonists, including all optical isomers, are prohibited. Most inhaled beta-2 agonists are prohibited, including arformoterol (Brovana), fenoterol, higenamine (norcoclaurine, Tinospora crispa), indacaterol (Arcapta), levalbuterol (Xopenex), metaproternol (Alupent), orciprenaline, olodaterol (Striverdi), pirbuterol (Maxair), terbutaline (Brethaire), vilanterol (Breo). The only exceptions are albuterol, formoterol, and salmeterol by a metered-dose inhaler when used -
A10 Anabolic Steroids Hardcore Info
CONTENTS GENERAL INFORMATION 3 Anabolic steroids – What are they? 4 How do they Work? – Aromatisation 5 More molecules – More problems 6 The side effects of anabolic steroids 7 Women and anabolic steroids 8 Injecting steroids 9 Abscesses – Needle Exchanges 10 Intramuscular injection 11 Injection sites 12 Oral steroids – Cycles – Stacking 13 Diet 14 Where do steroids come from? Spotting a counterfeit 15 Drug Information – Drug dosage STEROIDS 16 Anadrol – Andriol 17 Anavar – Deca-Durabolin 18 Dynabolon – Durabolin – Dianabol 19 Esiclene – Equipoise 20 Primobolan Depot – Proviron – Primobolan orals – Pronobol 21 Sustanon – Stromba, Strombaject – Testosterone Cypionate Testosterone Enanthate 22 Testosterone Propionate – Testosterone Suspension 23 Trenbolone Acetate – Winstrol OTHER DRUGS 24 Aldactone – Arimidex 25 Clenbuterol – Cytomel 26 Ephedrine Hydrochloride – GHB 27 Growth Hormone 28 Insulin 30 Insulin-Like Growth Factor-1 – Human Chorionic Gonadotrophin 31 Tamoxifen – Nubain – Recreational Drugs 32 Steroids and the Law 34 Glossary ANABOLIC STEROIDS People use anabolic steroids for various reasons, some use them to build muscle for their job, others just want to look good and some use them to help them in sport or body building. Whatever the reason, care needs to be taken so that as little harm is done to the body as possible because despite having muscle building effects they also have serious side effects especially when used incorrectly. WHAT ARE THEY? Anabolic steroids are man made versions of the hormone testosterone. Testosterone is the chemical in men responsible for facial hair, deepening of the voice and sex organ development, basically the masculine things Steroids are in a man. used in medicine to treat anaemia, muscle weakness after These masculine effects surgery etc, vascular are called the androgenic disorders and effects of testosterone. -
2010 Prohibited List
The World Anti-Doping Code THE 2010 PROHIBITED LIST INTERNATIONAL STANDARD The official text of the Prohibited List shall be maintained by WADA and shall be published in English and French. In the event of any conflict between the English and French versions, the English version shall prevail. This List shall come into effect on 1 January 2010 The Prohibited List 2010 19 September 2009 THE 2010 PROHIBITED LIST WORLD ANTI-DOPING CODE Valid 1 January 2010 All Prohibited Substances shall be considered as “Specified Substances” except Substances in classes S1, S2.1 to S2.5, S.4.4 and S6.a, and Prohibited Methods M1, M2 and M3. SUBSTANCES AND METHODS PROHIBITED AT ALL TIMES (IN- AND OUT-OF-COMPETITION) PROHIBITED SUBSTANCES S1. ANABOLIC AGENTS Anabolic agents are prohibited. 1. Anabolic Androgenic Steroids (AAS) a. Exogenous* AAS, including: 1-androstendiol (5α-androst-1-ene-3β,17β-diol ); 1-androstendione (5α- androst-1-ene-3,17-dione); bolandiol (19-norandrostenediol); bolasterone; boldenone; boldione (androsta-1,4-diene-3,17-dione); calusterone; clostebol; danazol (17α-ethynyl-17β-hydroxyandrost-4-eno[2,3-d]isoxazole); dehydrochlormethyltestosterone (4-chloro-17β-hydroxy-17α-methylandrosta- 1,4-dien-3-one); desoxymethyltestosterone (17α-methyl-5α-androst-2-en- 17β-ol); drostanolone; ethylestrenol (19-nor-17α-pregn-4-en-17-ol); fluoxymesterone; formebolone; furazabol (17β-hydroxy-17α-methyl-5α- androstano[2,3-c]-furazan); gestrinone; 4-hydroxytestosterone (4,17β- dihydroxyandrost-4-en-3-one); mestanolone; mesterolone; metenolone; methandienone -
Land Under Pressure: the Value of Irish Land in a Period of Rapid Population Growth, 1730–1844*
Land under pressure: The value of Irish land in a period of rapid population growth, 1730–1844* land under pressure by Peter M. Solar and Luc Hens Abstract This paper uses information on almost 5000 leases to arrive at estimates for the trends in current land values in County Armagh from 1730 to 1844. The estimates control for the length of the lease, the holding size, and the quality of land in the townland where the property was located, the last relying on information from the General Valuation of Ireland. They show growth in nominal rents up to the early 1770s, a plateau in the 1770s, 1780s and 1790s, an increase to the early 1810s, followed by a fall to the early 1820s and another plateau thereafter, stretching until the famine of the late 1840s. Taken together with information on wage and price trends, the new estimates show little change in real rents and negative total factor productivity growth from the 1780s to the 1830s. The Irish economy in the eighteenth and early nineteenth centuries was predominantly agricultural. In 1841, 53 per cent of the labour force worked on the land, and in the early eighteenth century the share was probably higher.1 The timing and direction of change in the intervening years are a matter of dispute, which is unlikely ever to be resolved fully in the absence of sufficiently reliable statistical information.2 Ireland was also experiencing one of the highest population growth rates in Europe: from the early 1750s until the 1820s upwards of 1.4 per cent per annum.3 The natural rate of population growth remained relatively high into the 1830s and early 1840s, with the actual rate slowing only with the beginnings of mass emigration. -
ALL the PRETTY HORSES.Hwp
ALL THE PRETTY HORSES Cormac McCarthy Volume One The Border Trilogy Vintage International• Vintage Books A Division of Random House, Inc. • New York I THE CANDLEFLAME and the image of the candleflame caught in the pierglass twisted and righted when he entered the hall and again when he shut the door. He took off his hat and came slowly forward. The floorboards creaked under his boots. In his black suit he stood in the dark glass where the lilies leaned so palely from their waisted cutglass vase. Along the cold hallway behind him hung the portraits of forebears only dimly known to him all framed in glass and dimly lit above the narrow wainscotting. He looked down at the guttered candlestub. He pressed his thumbprint in the warm wax pooled on the oak veneer. Lastly he looked at the face so caved and drawn among the folds of funeral cloth, the yellowed moustache, the eyelids paper thin. That was not sleeping. That was not sleeping. It was dark outside and cold and no wind. In the distance a calf bawled. He stood with his hat in his hand. You never combed your hair that way in your life, he said. Inside the house there was no sound save the ticking of the mantel clock in the front room. He went out and shut the door. Dark and cold and no wind and a thin gray reef beginning along the eastern rim of the world. He walked out on the prairie and stood holding his hat like some supplicant to the darkness over them all and he stood there for a long time. -
Monmouth Boat Club Has Diamond Jubilee
For All Departments Call RED BANK REGISTER RE 6-0013 VOLUME LXXVI, NO. 49 RED BANK, N. J., THURSDAY, JUNE 3, 1954 10c PER COPY SECTION ONE—PAGES 1 TO 16. Coast Guard Auxiliary to Hold Oceanic Fire Company Celebrates 75th Anniversary Monmouth Boat Club Courtesy Exams This Week-End Has Diamond Jubilee ••- NEW YORK CITY—Roar Ad- This is the diamond jubilee year miral Louis B. Olson, commander save him a g d deal of trouble if of thn Monmouth Boat club cover- of the Coast Guard's eastern area he is inspected later by a regular State Chamber ing 73 years of boating activities. and third Coast Guard district, this Federal Communications commis- Its anniversary program, the birth- week called attention to all pleas- sion official. This check is also day date being May 29, culminates ure boat.owners to the free public rendered as a "courteBy," and no Opposes Bill 9 with thin week's activities. service of the Coast Guard auxili- report Is made to F.C.C. should it The club has Issued a souvenir ary in conducting safety examina- be found that the boat owner has history and roster, 'he introductory tions of pleasure boats. not fully complied with the laws On Teacher Pay page of which carried a message "This season," he said, "presents pertaining to vessel radio stations. from Commodoro Harvey N, a. greator challenge than ever be- Each auxiliary flotilla has its Commissioner Schcnck as follows: fore. Many new boat owners are corps of qualified examiners and "Tho Monmouth Boat club's his- venturing on the waters for the will sponsor certain localities in its Isn't Arbiter, tory over the past 75 years reveals first time with little or no experi- immediate vicinity. -
WO 2012/167278 Al 6 December 2012 (06.12.2012) P O P C T
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2012/167278 Al 6 December 2012 (06.12.2012) P O P C T (51) International Patent Classification: [RU/GB]; 6 Kilwarlin Crescent, Hillsborough, Northern G01N 33/50 (2006.01) C12Q 1/68 (2006.01) Ireland BT26 6QF (GB). KENNEDY, Richard [GB/GB]; 15 Edgcumbe Gardens, Belfast, Northern Ireland BT4 2EG (21) International Application Number: (GB). DAVISON, Timothy [US/US]; 301 Washington PCT/US20 12/040805 Street, Apt. 2109, Conshohocken, PA 19428 (US). (22) International Filing Date: WINTER, Andreas [DE/DE]; Wallensteinstrasse 19, 4 June 2012 (04.06.2012) 86368 Gersthofen (DE). MCCAVIGAN, Andrena [IE/IE]; 25 Pier Rampart, Derryadd, Lurgan, County (25) Filing Language: English Armagh, BT66 6QH (IE). (26) Publication Language: English (74) Agent: NLX, F., Brent; Johnson, Marcou & Isaacs, LLC, (30) Priority Data: 317a. E. Liberty Street, Savannah, GA 31401 (US). 61/492,488 2 June 201 1 (02.06.201 1) US (81) Designated States (unless otherwise indicated, for every (71) Applicant (for all designated States except US): ALMAC kind of national protection available): AE, AG, AL, AM, DIAGNOSTICS LIMITED [IE/GB]; Almac House, 20 AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, Seagoe Industrial Estate, Craigavon, Northern Ireland CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, BT63 5QD (GB). DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, (72) Inventors; and KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, (75) Inventors/Applicants (for US only): HARKIN, Dennis, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, Paul [IE/GB]; 195 Ballygowan Road, Dromore Co. -
伊域化學藥業(香港)有限公司 Cyclopentyl Substituted Compounds
® 伊 域 化 學(香 藥 香 港) 業 港有 有 限 限 公 公 司 司 YICK-VIC CHEMICALS & PHARMACEUTICALS (HK) LTD Rm 1006, 10/F, Hewlett Centre, Tel: (852) 25412772 (4 lines) No. 52-54, Hoi Yuen Road, Fax: (852) 25423444 / 25420530 / 21912858 Kwun Tong, E-mail: [email protected] YICKYICK----VICVICVICVIC 伊域伊域伊域 Kowloon, Hong Kong. Site: http://www.yickvic.com Cyclopentyl Substituted Compounds Product Code CAS Product Name CC-0052BA 39746-00-4 (-)-COREY LACTONE BENZOATE CC-3702AD 1211-29-6 (-)-METHYL JASMONATE SPI-4467C 87269-86-1 (-)-OCTAHYDROCYCLOPENTA[B]PYRROLE-2-CARBOXYLIC ACID HYDROCHLORIDE SPI-4467A 93779-30-7 (+/-)-OCTAHYDROCYCLOPENTA[B]PYRROLE-2-CARBOXYLIC ACID HYDROCHLORIDE SPI-2956FG 142217-81-0 (1S,3R,4S)-2-AMINO-9-(4-BENZYLOXY)-3-(BENZYLOXYMETHYL)-2-METHYLIDENECYCLOPENTYL-3H-PURINE-9-ONE SPI-2956FJ 142217-78-5 (2R,3S,5S)-3-(BENZYLOXY)-5-[2-[[(4-METHOXYPHENYL)DIPHENYLMETHYL]AMINO]-6-(PHENYLMETHOXY)-9H-PURIN-9-Y L]-2-(BENZYLOXYMETHYL)CYCLOPENTANOL SPI-1560DH 4167-77-5 1,1-CYCLOPENTANEDICARBOXYLIC ACID DIETHYL ESTER SPI-2808C 5763-44-0 1,2-CYCLOPENTANE DICARBOXIMIDE CC-1957B 1222-05-5 1,3,4,6,7,8-HEXAHYDRO-4,6,6,7,8,8-HEXAMETHYLCYCLOPENTA[G]-2-BENZOPYRAN SPI-1246B 3859-41-4 1,3-CYCLOPENTANEDIONE SPI-1955AA 646-06-0 1,3-DIOXOLANE SPI-3082AA 54078-29-4 1,8-DIAZAFLUOREN-9-ONE UNIE-13864 564-35-2 11-KETOTESTOSTERONE SPI-0098CA 640-87-9 17-ALPHA,21-DIHYDROXYPREGN-4-ENE-3,20-DIONE 21-ACETATE UNIE-13126 302-76-1 17ALPHA-METHYL-17BETA-ESTRADIOL UNIE-14695 3563-27-7 17BETA-DIHYDROQUILIN UNIE-2836 10316-79-7 1-AMINO-1-CYCLOPENTANEMETHANOL SPI-3077CB 61379-64-4 1-AMINO-4-CYCLOPENTYLPIPERAZINE -
Geographic Names
GEOGRAPHIC NAMES CORRECT ORTHOGRAPHY OF GEOGRAPHIC NAMES ? REVISED TO JANUARY, 1911 WASHINGTON GOVERNMENT PRINTING OFFICE 1911 PREPARED FOR USE IN THE GOVERNMENT PRINTING OFFICE BY THE UNITED STATES GEOGRAPHIC BOARD WASHINGTON, D. C, JANUARY, 1911 ) CORRECT ORTHOGRAPHY OF GEOGRAPHIC NAMES. The following list of geographic names includes all decisions on spelling rendered by the United States Geographic Board to and including December 7, 1910. Adopted forms are shown by bold-face type, rejected forms by italic, and revisions of previous decisions by an asterisk (*). Aalplaus ; see Alplaus. Acoma; township, McLeod County, Minn. Abagadasset; point, Kennebec River, Saga- (Not Aconia.) dahoc County, Me. (Not Abagadusset. AQores ; see Azores. Abatan; river, southwest part of Bohol, Acquasco; see Aquaseo. discharging into Maribojoc Bay. (Not Acquia; see Aquia. Abalan nor Abalon.) Acworth; railroad station and town, Cobb Aberjona; river, IVIiddlesex County, Mass. County, Ga. (Not Ackworth.) (Not Abbajona.) Adam; island, Chesapeake Bay, Dorchester Abino; point, in Canada, near east end of County, Md. (Not Adam's nor Adams.) Lake Erie. (Not Abineau nor Albino.) Adams; creek, Chatham County, Ga. (Not Aboite; railroad station, Allen County, Adams's.) Ind. (Not Aboit.) Adams; township. Warren County, Ind. AJjoo-shehr ; see Bushire. (Not J. Q. Adams.) Abookeer; AhouJcir; see Abukir. Adam's Creek; see Cunningham. Ahou Hamad; see Abu Hamed. Adams Fall; ledge in New Haven Harbor, Fall.) Abram ; creek in Grant and Mineral Coun- Conn. (Not Adam's ties, W. Va. (Not Abraham.) Adel; see Somali. Abram; see Shimmo. Adelina; town, Calvert County, Md. (Not Abruad ; see Riad. Adalina.) Absaroka; range of mountains in and near Aderhold; ferry over Chattahoochee River, Yellowstone National Park.