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CHAPTER 41 Target Genes: Bone Proteins 719 CHAPTER 41 Target Genes: Bone Proteins 719 45. Bellows CG, Reimers SM, Heersche JN 1999 Expression of 61. Price PA, Williamson MK, Lothringer JW 1981 Origin of the mRNAs for type-I collagen, bone sialoprotein, osteocalcin, vitamin K-dependent bone protein found in plasma and its and osteopontin at different stages of osteoblastic differentia- clearance by kidney and bone. J Biol Chem 256:12760–12766. tion and their regulation by 1,25-dihydroxyvitamin D3. Cell 62. Ducy P, Desbois C, Boyce B, Pinero G, Story B, Dunstan C, Tissue Res 297:249–259. Smith E, Bonadio J, Goldstein S, Gundberg C, Bradley A, 46. Broess M, Riva A, Gerstenfeld LC 1995 Inhibitory effects of Karsenty G 1996 Increased bone formation in osteocalcin- 1,25(OH)2 vitamin D3 on collagen type I, osteopontin, and deficient mice. Nature 382:448–452. osteocalcin gene expression in chicken osteoblasts. J Cell 63. Chenu C, Colucci S, Grano M, Zigrino P, Barattolo R, Biochem 57:440–451. Zambonin G, Baldini N, Vergnaud P, Delmas PD, Zallone AZ 47. Yoon K, Buenaga R, Rodan GA, Prince CW, Butler WT 1994 Osteocalcin induces chemotaxis, secretion of matrix pro- 1987 Tissue specificity and developmental expression of teins, and calcium-mediated intracellular signaling in human rat osteopontin. Biochem Biophys Res Commun 148: osteoclast-like cells. J Cell Biol 127:1149–1158. 1129–1136. 64. Watts NB 1999 Clinical utility of biochemical markers of bone 48. Beresford JN, Joyner CJ, Devlin C, Triffitt JT 1994 The effects remodeling. Clin Chem 45:1359–1368. of dexamethasone and 1,25-dihydroxyvitamin D3 on osteogenic 65. Ivaska KK, Hentunen TA, Vaaraniemi J, Ylipahkala H, differentiation of human marrow stromal cells in vitro. Arch Pettersson K, Vaananen HK 2004 Release of intact and frag- Oral Biol 39:941–947. mented osteocalcin molecules from bone matrix during bone 49. Prince CW, Butler WT 1987 1,25-Dihydroxyvitamin D3 regu- resorption in vitro. J Biol Chem 279:18361–18369. lates the biosynthesis of osteopontin, a bone-derived cell 66. Beresford JN, Gallagher JA, Poser JW, Russell RG 1984 attachment protein, in clonal osteoblast-like osteosarcoma Production of osteocalcin by human bone cells in vitro. cells. Coll Relat Res 7:305–313. Effects of 1,25(OH)2D3, 24,25(OH)2D3, parathyroid hormone, 50. Chen J, Thomas HF, Sodek J, Kim RH, Ogata Y 1996 Regulation and glucocorticoids. Metab Bone Dis Relat Res 5:229–234. of bone sialoprotein and osteopontin mRNA expression by 67. Lian J, Stewart C, Puchacz E, Mackowiak S, Shalhoub V, dexamethasone and 1,25-dihydroxyvitamin D3 in rat bone Collart D, Zambetti G, Stein G 1989 Structure of the rat osteo- organ cultures. Connect Tissue Res 34:41–51. calcin gene and regulation of vitamin D-dependent expression. 51. Matsue M, Kageyama R, Denhardt DT, Noda M 1997 Helix– Proc Natl Acad Sci USA 86:1143–1147. loop–helix-type transcription factor (HES-1) is expressed in 68. Lajeunesse D, Kiebzak GM, Frondoza C, Sacktor B 1991 osteoblastic cells, suppressed by 1,25(OH)2 vitamin D3, and Regulation of osteocalcin secretion by human primary bone modulates 1,25(OH)2 vitamin D3 enhancement of osteopontin cells and by the human osteosarcoma cell line MG-63. Bone gene expression. Bone 20:329–334. Miner 14:237–250. 52. Noda M, Rodan GA 1989 Transcriptional regulation of osteo- 69. Ingram RT, Bonde SK, Riggs BL, Fitzpatrick LA 1994 Effects pontin production in rat osteoblast-like cells by parathyroid of transforming growth factor beta (TGF beta) and 1,25-dihy- hormone. J Cell Biol 108:713–718. droxyvitamin D3 on the function, cytochemistry and morphol- 53. Staal A, Van Wijnen AJ, Desai RK, Pols HA, Birkenhager JC, ogy of normal human osteoblast-like cells. Differentiation Deluca HF, Denhardt DT, Stein JL, Van Leeuwen JP, Stein GS, 55:153–163. Lian JB 1996 Antagonistic effects of transforming growth 70. Bodine PV, Trailsmith M, Komm BS 1996 Development and factor-beta on vitamin D3 enhancement of osteocalcin and characterization of a conditionally transformed adult human osteopontin transcription: reduced interactions of vitamin D osteoblastic cell line. J Bone Miner Res 11:806–819. receptor/retinoid X receptor complexes with vitamin E response 71. Mosavin R, Mellon WS 1996 Posttranscriptional regulation of elements. Endocrinology 137:2001–2011. osteocalcin mRNA in clonal osteoblast cells by 1,25-dihydroxy- 54. Ganss B, Kim RH, Sodek J 1999 Bone sialoprotein. Crit Rev vitamin D3. 574.1905 A67 Arch Biochem Biophys 332:142–152. Oral Biol Med 10:79–98. 72. Hicok KC, Thomas T, Gori F, Rickard DJ, Spelsberg TC, 55. Oldberg A, Jirskog-Hed B, Axelsson S, Heinegard D 1989 Riggs BL 1998 Development and characterization of condi- Regulation of bone sialoprotein mRNA by steroid hormones. tionally immortalized osteoblast precursor cell lines from J Cell Biol 109:3183–3186. human bone marrow stroma. J Bone Miner Res 13:205–217. 56. Li JJ, Sodek J 1993 Cloning and characterization of the rat 73. Lajeunesse D, Frondoza C, Schoffield B, Sacktor B 1990 bone sialoprotein gene promoter. Biochem J 289:625–629. Osteocalcin secretion by the human osteosarcoma cell line 57. Kim RH, Li JJ, Ogata Y, Yamauchi M, Freedman LP 1996 MG-63. J Bone Miner Res 5:915–922. Identification of a vitamin D3-response element that overlaps 74. Hosoda K, Kanzaki S, Eguchi H, Kiyoki M, Yamaji T, a unique inverted TATA box in the rat bone sialoprotein gene. Koshihara Y, Shiraki M, Seino Y 1993 Secretion of osteocalcin Biochem J 318:219–226. and its propeptide from human osteoblastic cells: Dissociation 58. Sodek J, Kim RH, Ogata Y, Li J, Yamauchi M, Zhang Q, of the secretory patterns of osteocalcin and its propeptide. Freedman LP 1995 Regulation of bone sialoprotein gene J Bone Miner Res 8:553–565. transcription by steroid hormones. Connect Tissue Res 32: 75. Zhang R, Ducy P, Karsenty G 1997 1,25-Dihydroxyvitamin D3 209–217. inhibits osteocalcin expression in mouse through an indirect 59. Chang PL, Prince CW 1991 1α,25-Dihydroxyvitamin D3 stim- mechanism. J Biol Chem 272:110–116. ulates synthesis and secretion of nonphosphorylated osteopon- 76. Lian JB, Shalhoub V, Aslam F, Frenkel B, Green J, Hamrah M, tin (secreted phosphoprotein 1) in mouse JB6 epidermal cells. Stein GS, Stein JL 1997 Species-specific glucocorticoid and Cancer Res 51:2144–2150. 1,25-dihydroxyvitamin D responsiveness in mouse MC3T3-E1 60. Pockwinse SM, Stein JL, Lian JB, Stein GS 1995 Developmental osteoblasts: Dexamethasone inhibits osteoblast differentiation stage-specific cellular responses to vitamin D and glucocorti- and vitamin D down-regulates osteocalcin gene expression. coids during differentiation of the osteoblast phenotype: Endocrinology 138:2117–2127. Interrelationship of morphology and gene expression by in situ 77. Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, hybridization. Exp Cell Res 216:244–260. Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, 720 PAUL H. ANDERSON ET AL. Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T 1997 86. Imai K, Neuman MW, Kawase T, Saito S 1992 Calcium in Targeted disruption of Cbfa1 results in a complete lack of bone osteoblast-enriched bone cells. Bone 13:217–223. formation owing to maturational arrest of osteoblasts. Cell 87. Halstead LR, Scott MJ, Rifas L, Avioli LV 1992 Characterization 89:755–764. of osteoblast-like cells from normal adult rat femoral trabecular 78. Otto F, Thornell AP, Crompton T, Denzel A, Gilmour KC, bone. Calcif Tissue Int 50:93–95. Rosewell IR, Stamp GW, Beddington RS, Mundlos S, Olsen BR, 88. El-Maadawy S, Kaartinen MT, Schinke T, Murshed M, Selby PB, Owen MJ 1997 Cbfa1, a candidate gene for Karsenty G, McKee MD 2003 Cartilage formation and calcifi- cleidocranial dysplasia syndrome, is essential for osteoblast cation in arteries of mice lacking matrix Gla protein. Connect differentiation and bone development. Cell 89:765–771. Tissue Res 44:272–278. 79. Javed A, Gutierrez S, Montecino M, van Wijnen AJ, Stein JL, 89. Fraser JD, Otawara Y, Price PA 1988 1,25-Dihydroxyvitamin D3 Stein GS, Lian JB 1999 Multiple Cbfa/AML sites in the rat stimulates the synthesis of matrix gamma-carboxyglutamic osteocalcin promoter are required for basal and vitamin D– acid protein by osteosarcoma cells. Mutually exclusive expression responsive transcription and contribute to chromatin organiza- of vitamin K–dependent bone proteins by clonal osteoblastic cell tion. Mol Cell Biol 19:7491–7500. lines. J Biol Chem 263:911–916. 80. Aslam F, McCabe L, Frenkel B, van Wijnen AJ, Stein GS, 90. Fraser JD, Price PA 1990 Induction of matrix Gla protein Lian JB, Stein JL 1999 AP-1 and vitamin D receptor (VDR) synthesis during prolonged 1,25-dihydroxyvitamin D3 treatment signaling pathways converge at the rat osteocalcin VDR of osteosarcoma cells. Calcif Tissue Int 46:270–279. element: Requirement for the internal activating protein-1 site 91. Barone LM, Owen TA, Tassinari MS, Bortell R, Stein GS, for vitamin D–mediated trans-activation. Endocrinology 140: Lian JB 1991 Developmental expression and hormonal regula- 63–70. tion of the rat matrix Gla protein (MGP) gene in chondrogenesis 81. Grigoriadis AE, Schellander K, Wang ZQ, Wagner EF 1993 and osteogenesis. J Cell Biochem 46:351–365. Osteoblasts are target cells for transformation in c-fos trans- 92. Hewison M, Zehnder D, Bland R, Stewart PM 2000 genic mice. J Cell Biol 122:685–701. 1α-Hydroxylase and the action of vitamin D. J Mol Endocrinol 82. McCabe LR, Banerjee C, Kundu R, Harrison RJ, Dobner PR 25:141–148. 1996 Developmental expression and activities of specific fos 93. Turner RT, Howard GA, Puzas JE, Baylink DJ, Knapp DR and jun proteins are functionally related to osteoblast maturation: 1983 Calvarial cells synthesize 1α,25-dihydroxyvitamin D3 Role of Fra-2 and Jun D during differentiation.
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