Isolation and Characterization of a Novel Sphingobium Yanoikuyae
F1000Research 2020, 9:767 Last updated: 07 SEP 2020 RESEARCH ARTICLE Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources [version 1; peer review: 2 approved] Mautusi Mitra 1, Kevin Manoap-Anh-Khoa Nguyen1,2, Taylor Wayland Box1, Jesse Scott Gilpin1, Seth Ryan Hamby1, Taylor Lynne Berry3,4, Erin Harper Duckett1 1Biology Department, University of West Georgia, Carrollton, GA, 30118, USA 2Department of Mechanical Engineering, Kennesaw State University, Marietta, GA, 30060, USA 3Carrollton High School, Carrollton, GA, 30117, USA 4Department of Chemistry and Biochemistry, University of North Georgia, Dahlonega, GA, 30597, USA v1 First published: 24 Jul 2020, 9:767 Open Peer Review https://doi.org/10.12688/f1000research.25284.1 Latest published: 24 Jul 2020, 9:767 https://doi.org/10.12688/f1000research.25284.1 Reviewer Status Abstract Invited Reviewers Background: Green micro-alga, Chlamydomonas reinhardtii (a Chlorophyte), can be cultured in the laboratory heterotrophically or 1 2 photo-heterotrophically in Tris-Phosphate-Acetate (TAP) medium, which contains acetate as the carbon source. Chlamydomonas can version 1 convert acetate in the TAP medium to glucose via the glyoxylate cycle, 24 Jul 2020 report report a pathway present in many microbes and higher plants. A novel bacterial strain, CC4533, was isolated from a contaminated TAP agar 1. David Dewez, University of Quebec at medium culture plate of a Chlamydomonas wild type strain. In this article, we present our research on the isolation, and biochemical and Montreal, Montreal, Canada molecular characterizations of CC4533. 2. Arun Karnwal , Lovely Professional Methods: We conducted several microbiological tests and spectrophotometric analyses to biochemically characterize CC4533.
[Show full text]