DAP3 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science DAP3 Rabbit pAb Catalog No.: A7003 Basic Information Background Catalog No. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in A7003 protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% Observed MW protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is 46kDa reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins Calculated MW comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical 41kDa/45kDa properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by Category tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Primary antibody Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on Applications chromosomes 1q and 2q. WB Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 7818 P51398 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 159-398 of human DAP3 (NP_387506.1). Synonyms DAP3;DAP-3;MRP-S29;MRPS29;bMRP-10;S29mt Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using DAP3 Antibody (A7003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Role of Mitochondrial Ribosomal Protein S18-2 in Cancerogenesis and in Regulation of Stemness and Differentiation
From THE DEPARTMENT OF MICROBIOLOGY TUMOR AND CELL BIOLOGY (MTC) Karolinska Institutet, Stockholm, Sweden ROLE OF MITOCHONDRIAL RIBOSOMAL PROTEIN S18-2 IN CANCEROGENESIS AND IN REGULATION OF STEMNESS AND DIFFERENTIATION Muhammad Mushtaq Stockholm 2017 All previously published papers were reproduced with permission from the publisher. Published by Karolinska Institutet. Printed by E-Print AB 2017 © Muhammad Mushtaq, 2017 ISBN 978-91-7676-697-2 Role of Mitochondrial Ribosomal Protein S18-2 in Cancerogenesis and in Regulation of Stemness and Differentiation THESIS FOR DOCTORAL DEGREE (Ph.D.) By Muhammad Mushtaq Principal Supervisor: Faculty Opponent: Associate Professor Elena Kashuba Professor Pramod Kumar Srivastava Karolinska Institutet University of Connecticut Department of Microbiology Tumor and Cell Center for Immunotherapy of Cancer and Biology (MTC) Infectious Diseases Co-supervisor(s): Examination Board: Professor Sonia Lain Professor Ola Söderberg Karolinska Institutet Uppsala University Department of Microbiology Tumor and Cell Department of Immunology, Genetics and Biology (MTC) Pathology (IGP) Professor George Klein Professor Boris Zhivotovsky Karolinska Institutet Karolinska Institutet Department of Microbiology Tumor and Cell Institute of Environmental Medicine (IMM) Biology (MTC) Professor Lars-Gunnar Larsson Karolinska Institutet Department of Microbiology Tumor and Cell Biology (MTC) Dedicated to my parents ABSTRACT Mitochondria carry their own ribosomes (mitoribosomes) for the translation of mRNA encoded by mitochondrial DNA. The architecture of mitoribosomes is mainly composed of mitochondrial ribosomal proteins (MRPs), which are encoded by nuclear genomic DNA. Emerging experimental evidences reveal that several MRPs are multifunctional and they exhibit important extra-mitochondrial functions, such as involvement in apoptosis, protein biosynthesis and signal transduction. Dysregulations of the MRPs are associated with severe pathological conditions, including cancer.
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IPS-1 Is Crucial for DAP3-Mediated Anoikis Induction by Caspase-8 Activation
Cell Death and Differentiation (2009) 16, 1615–1621 & 2009 Macmillan Publishers Limited All rights reserved 1350-9047/09 $32.00 www.nature.com/cdd IPS-1 is crucial for DAP3-mediated anoikis induction by caspase-8 activation H-M Li1, D Fujikura1, T Harada1, J Uehara1, T Kawai2,3, S Akira2,3, JC Reed4, A Iwai1 and T Miyazaki*,1 Detachment of adherent epithelial cells from the extracellular matrix induces apoptosis, a process known as anoikis. We have shown that DAP3 is critical for anoikis induction. However, the mechanism for anoikis induction mediated by DAP3 is still unclear. Here, we show that interferon-b promoter stimulator 1 (IPS-1) binds DAP3 and induces anoikis by caspase activation. Recently, IPS-1 has been shown to be critical for antiviral immune responses, although there has been no report of its function in apoptosis induction. We show that overexpression of IPS-1 induces apoptosis by activation of caspase-3, -8, and -9. In addition, IPS-1 knockout mouse embryonic ﬁbroblasts were shown to be resistant to anoikis. Interestingly, IPS-1 expression, recruitment of caspase-8 to IPS-1, and caspase-8 activation were induced after cell detachment. Furthermore, DAP3-mediated anoikis induction was inhibited by knockdown of IPS-1 expression. Therefore, we elucidated a novel function of IPS-1 for anoikis induction by caspase-8 activation. Cell Death and Differentiation (2009) 16, 1615–1621; doi:10.1038/cdd.2009.97; published online 31 July 2009 Apoptosis, the programmed physiological cell death, is critical induces programmed cell death, a phenomenon known as for modeling tissues and maintaining homeostasis in multi- anoikis.
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Hnoa1 Interacts with Complex I and DAP3 and Regulates
Supplemental Material can be found at: http://www.jbc.org/cgi/content/full/M807797200/DC1 THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 284, NO. 8, pp. 5414–5424, February 20, 2009 © 2009 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in the U.S.A. hNOA1 Interacts with Complex I and DAP3 and Regulates Mitochondrial Respiration and Apoptosis*□S Received for publication, October 9, 2008, and in revised form, December 15, 2008 Published, JBC Papers in Press, December 22, 2008, DOI 10.1074/jbc.M807797200 Tingdong Tang‡, Bin Zheng‡1, Sheng-hong Chen‡§, Anne N. Murphy¶, Krystyna Kudlicka‡, Huilin Zhou‡§, and Marilyn G. Farquhar‡2 From the Departments of ‡Cellular and Molecular Medicine and ¶Pharmacology and §Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093-0651 Mitochondria are dynamic organelles that play key roles in fusion proteins such as the dynamin like large G proteins Drp1 metabolism, energy production, and apoptosis. Coordination of and Opa1, the cell’s susceptibility to apoptotic agents (3) or these processes is essential to maintain normal cellular func- ability to generate ATP (4, 5) is altered. tions. Here we characterized hNOA1, the human homologue of Apoptosis is controlled by a diverse range of cell signals, AtNOA1 (Arabidopsis thaliana nitric oxide-associated protein which may originate either extracellularly (extrinsic inducers) Downloaded from 1), a large mitochondrial GTPase. By immunofluorescence, or intracellularly (intrinsic inducers), and mitochondria play immunoelectron microscopy, and mitochondrial subfraction- central roles in both pathways (6). The apoptotic pathways ation, endogenous hNOA1 is localized within mitochondria involve a growing list of mitochondria-associated proteins, where it is peripherally associated with the inner mitochondrial such as Bad, cytochrome c, Smac, AIF, Bcl-2, and others, most membrane facing the mitochondrial matrix.
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DAP3 Antibody A
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DAP3 Mouse Monoclonal Antibody [Clone ID: OTI2D4] Product Data
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And Fas-Induced Cell Death
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DAP3 Antibody
Product Datasheet DAP3 Antibody Catalog No: #31066 Package Size: #31066-1 50ul #31066-2 100ul Orders: [email protected] Support: [email protected] Description Product Name DAP3 Antibody Host Species Rabbit Clonality Polyclonal Applications ELISA WB IHC Species Reactivity Hu Specificity The antibody detects endogenous level of total DAP3 protein. Immunogen Type Recombinant protein Immunogen Description Fusion protein corresponding to a C terminal 300 amino acids of human death associated protein 3 Target Name DAP3 Other Names death associated protein 3, DAP-3, MRPS29, MRP-S29, bMRP-10 Accession No. Swiss-Prot:Q16828Gene ID:1848 Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. Storage Store at -20°C/1 year Application Details Predicted MW: 46kd ELISA: 1:1000-1:5000 Western blotting: 1:200-1:1000 Immunohistochemistry: 1:5-1:20 Images Gel: 12%SDS-PAGE Lane1: 293T cell lysate Lane2: Jurkat cell lysate Lane3: Human Lymphoma tissue lysate Lysates: 30ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31066 (DAP3 Antibody) at dilution 1/10, on the right is treated with the fusion protein. Background This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27.
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