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DAP3 Antibody Cat. No.: 22-624 DAP3 Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 159-398 IMMUNOGEN: of human DAP3 (NP_387506.1). TESTED APPLICATIONS: WB APPLICATIONS: WB: ,1:500 - 1:2000 POSITIVE CONTROL: 1) MCF7 2) Jurkat 3) Mouse kidney 4) Mouse liver PREDICTED MOLECULAR Observed: 46kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal October 1, 2021 1 https://www.prosci-inc.com/dap3-antibody-22-624.html ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: DAP3 DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, ALTERNATE NAMES: MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29 GENE ID: 7818 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical BACKGROUND: properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 1, 2021 2 https://www.prosci-inc.com/dap3-antibody-22-624.html.

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Role of Mitochondrial Ribosomal Protein S18-2 in Cancerogenesis and in Regulation of Stemness and Differentiation
From THE DEPARTMENT OF MICROBIOLOGY TUMOR AND CELL BIOLOGY (MTC) Karolinska Institutet, Stockholm, Sweden ROLE OF MITOCHONDRIAL RIBOSOMAL PROTEIN S18-2 IN CANCEROGENESIS AND IN REGULATION OF STEMNESS AND DIFFERENTIATION Muhammad Mushtaq Stockholm 2017 All previously published papers were reproduced with permission from the publisher. Published by Karolinska Institutet. Printed by E-Print AB 2017 © Muhammad Mushtaq, 2017 ISBN 978-91-7676-697-2 Role of Mitochondrial Ribosomal Protein S18-2 in Cancerogenesis and in Regulation of Stemness and Differentiation THESIS FOR DOCTORAL DEGREE (Ph.D.) By Muhammad Mushtaq Principal Supervisor: Faculty Opponent: Associate Professor Elena Kashuba Professor Pramod Kumar Srivastava Karolinska Institutet University of Connecticut Department of Microbiology Tumor and Cell Center for Immunotherapy of Cancer and Biology (MTC) Infectious Diseases Co-supervisor(s): Examination Board: Professor Sonia Lain Professor Ola Söderberg Karolinska Institutet Uppsala University Department of Microbiology Tumor and Cell Department of Immunology, Genetics and Biology (MTC) Pathology (IGP) Professor George Klein Professor Boris Zhivotovsky Karolinska Institutet Karolinska Institutet Department of Microbiology Tumor and Cell Institute of Environmental Medicine (IMM) Biology (MTC) Professor Lars-Gunnar Larsson Karolinska Institutet Department of Microbiology Tumor and Cell Biology (MTC) Dedicated to my parents ABSTRACT Mitochondria carry their own ribosomes (mitoribosomes) for the translation of mRNA encoded by mitochondrial DNA. The architecture of mitoribosomes is mainly composed of mitochondrial ribosomal proteins (MRPs), which are encoded by nuclear genomic DNA. Emerging experimental evidences reveal that several MRPs are multifunctional and they exhibit important extra-mitochondrial functions, such as involvement in apoptosis, protein biosynthesis and signal transduction. Dysregulations of the MRPs are associated with severe pathological conditions, including cancer.
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IPS-1 Is Crucial for DAP3-Mediated Anoikis Induction by Caspase-8 Activation
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Hnoa1 Interacts with Complex I and DAP3 and Regulates
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DAP3 Antibody A
C 0 2 - t DAP3 Antibody a e r o t S Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) 2 7 Web: [email protected] 1 www.cellsignal.com 2 # 3 Trask Lane Danvers Massachusetts 01923 USA For Research Use Only. Not For Use In Diagnostic Procedures. Applications: Reactivity: Sensitivity: MW (kDa): Source: UniProt ID: Entrez-Gene Id: WB H Endogenous 46 Rabbit P51398 7818 Product Usage Information 5. Imataka, H. et al. (1997) EMBO J 16, 817-25. 6. Levy-Strumpf, N. et al. (1997) Mol Cell Biol 17, 1615-25. Application Dilution 7. Henis-Korenblit, S. et al. (2000) Mol Cell Biol 20, 496-506. Western Blotting 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Specificity / Sensitivity DAP3 Antibody detects endogenous levels of total DAP3 protein. The antibody does not cross-react with other DAP family members. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys58 of human DAP3. Antibodies are purified by protein A and peptide affinity chromatography. Background Death associated protein 1 (DAP1) is a 15 kDa protein that functions as a positive mediator of cell death initiated by interferon-gamma (1,2). The DAP1 protein is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). The protein is localized in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear.
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And Fas-Induced Cell Death
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DAP3 Antibody
Product Datasheet DAP3 Antibody Catalog No: #31066 Package Size: #31066-1 50ul #31066-2 100ul Orders: [email protected] Support: [email protected] Description Product Name DAP3 Antibody Host Species Rabbit Clonality Polyclonal Applications ELISA WB IHC Species Reactivity Hu Specificity The antibody detects endogenous level of total DAP3 protein. Immunogen Type Recombinant protein Immunogen Description Fusion protein corresponding to a C terminal 300 amino acids of human death associated protein 3 Target Name DAP3 Other Names death associated protein 3, DAP-3, MRPS29, MRP-S29, bMRP-10 Accession No. Swiss-Prot:Q16828Gene ID:1848 Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. Storage Store at -20°C/1 year Application Details Predicted MW: 46kd ELISA: 1:1000-1:5000 Western blotting: 1:200-1:1000 Immunohistochemistry: 1:5-1:20 Images Gel: 12%SDS-PAGE Lane1: 293T cell lysate Lane2: Jurkat cell lysate Lane3: Human Lymphoma tissue lysate Lysates: 30ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31066 (DAP3 Antibody) at dilution 1/10, on the right is treated with the fusion protein. Background This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27.
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