DOCSLIB.ORG

United States Patent (19) 11) Patent Number: 5,382,428 Livingston-Wheeler Et Al

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
United States Patent (19) 11) Patent Number: 5,382,428 Livingston-Wheeler Et Al US005382428A United States Patent (19) 11) Patent Number: 5,382,428 Livingston-Wheeler et al. 45 Date of Patent: Jan. 17, 1995 54 METHOD FOR PREPARNG A PURIFIED 56) References Cited EXTRACTION RESIDUE FRACTION AND TSUSE IN STMULATING THE IMMUNE U.S. PATENT DOCUMENTS RESPONSE 3,958,025 5/1976 Livingston .......................... 424/317 OTHER PUBLICATIONS 75 Inventors: Virginia Livingston-Wheeler, San Livingston, et al., Trans. N.Y. Acad. Sci., vol. 36, p. 569 Diego County, Calif.; John J. (1974). Majnarich, King County, Wash. Chen et al., Proc. Soc. Exp. Biol. and Med., vol. 152, pp. 408-410 (1976). (73 Assignees: John J. Mainarich; Virginia Maruo et al., Proc. Natl. Acad. Sci., vol. 76, pp. Livingston-Wheeler, both of 6622-6626, 1979. Redmond, Wash. Acevedo et al., Cancer, vol. 41, pp. 1217-1229, 1978. Acevedo et al., Injection and Immunity, vol. 24, pp. 920-924, 1979. 21 Appl. No.: 336,567 Primary Examiner-Douglas W. Robinson Assistant Examiner-Deborah K. Ware 22 Fled: Apr. 11, 1989 Attorney, Agent, or Firm-Seed and Berry 57 ABSTRACT Related U.S. Application Data A method is disclosed for preparing a purified extrac 63 Continuation of Ser. No. 150,512, Feb. 8, 1988, aban tion residue fraction (antigen) of a microorganism iso doned, which is a continuation of Ser. No. 935,123, lated from the blood or urine of a warm-blooded animal Nov. 26, 1986, abandoned, which is a continuation of or tumor carried by a warm-blooded animal, the micro Ser. No. 712,468, Mar. 15, 1985, abandoned, which is a organism having the capacity to synthesize the polypep continuation of Ser. No. 523,679, Aug. 16, 1983, aban tide hormone known as "chorionic gonadotropin' in its doned, which is a continuation-in-part of Ser. No. total form or in its alpha and beta subunits. The purified 255,678, Apr. 20, 1981, Pat. No. 4,410,510, which is a extraction residue fraction is an activator and modula continuation-in-part of Ser. No. 128,919, Mar. 10, 1980, tor of immunological response and is capable of evoking abandoned, which is a continuation of Ser. No. pronounced prophylactic and therapeutic effects 957,206, Nov. 3, 1978, abandoned. against a variety of tumors in laboratory animals and man. The immune response is stimulated by administer (51) Int. Cl. ..................... A61K 37/00; A61K 39/02; ing an effective immuno-stimulating amount of the resi C12N 1/20; A01N 37/18 due fraction and/or co-administering the residue frac (52) U.S. C. .............................. 424/234.1; 424/282.1; tion together with an adjuvant such as the complete 435/68. 1; 435/71.1; 435/252.1; 514/2 Freund's adjuvant. 58) Field of Search ................. 424/92, 93 D; 435/68, 435/252.1, 253, 71.7; 514/2 8 Claims, No Drawings 5,382,428 1. 2 Livingston and E. Alexander-Jackson, “A Specific METHOD FOR PRE PARING A PURIFED Type of Organism Cultivated from Malignancy: Bacte EXTRACTION RESOUE FRACTION AND ITS USE riology and Proposed Classification,” Ann. N. Y. Acad. NST MULATING THE MMUNE RESPONSE Sci., 174: 636-654 (1970); and V. W-C. Livingston and A. M. Livingston, "Some Cultural, Immunological and CROSS-REFERENCE TO RELATED Bio-Chemical Properties of Progenitor Cryptocides,” APPLICATION Trans. N. Y. Acad. Sci, 36:569-582 (1974). This application is a continuation of U.S. application The avian leukosis complex comprises the neoplastic Ser. No. 07/150,512, filed Feb. 8, 1988, now abandoned, diseases of the hematopoietic system of the domestic which is a continuation of U.S. patent application Ser. 10 chicken, together with several other neoplastic and No. 935,123, filed Nov. 26, 1986, now abandoned under non-neoplastic conditions which are related either etio C.F.R. 8 1.62, which is a continuation of U.S. patent logically or pathologically. The following diseases are application Ser. No. 712,468, filed Mar. 15, 1985, now included in the complex leukosis: lymphoid leukosis, abandoned, which was a continuation of U.S. patent erythroid leukosis, and myeloid leukosis. Diseases etio application Ser. No. 523,679, filed Aug. 16, 1983, now 15 logically related to leukosis include sarcoma, neophrob abandoned, which was a continuation-in-part (CIP) of lastoma, endothelioma and osteopletrosis. Marek's dis U.S. patent application Ser. No. 255,678, filed Apr. 20, ease includes the neural form, visceral form and ocular 1981, now U.S. Pat. No. 4410,510, which was a con form. See The Merck Veterinary Manual, 3rd edition, tinuation-in-part of U.S. Ser. No. 128,919, filed Mar. 10, Merck & Co., Inc., New Jersey, pp. 1081-1091 (1976). 1980, now abandoned, which was a continuation of U.S. Marek's disease, for example, is seen in birds three patent application Ser. No. 957,206, filed Nov. 3, 1978 weeks of age onward to adults. The commonest is two now abandoned. to four months. Young chicks are more susceptible than TECHNICAL FIELD older birds. Genetic factors strongly influence the re sponse of birds to agents of the leukosis complex. See A. This invention relates to a method of preparing a 25 E. Churchill and P. M. Briggs, “Agent of Marek's Dis water-soluble, purified extraction residue fraction (anti ease in Culture,” Nature, London 215: 28–530 (1967), gen) of a microorganism, the purified residue fraction and J. J. Solomon, R. L. Witter, K. Nazerian and B. B. itself, and its use alone or in combination with an adju Burmester, “Studies on the Etiology of Marek's Dis vant for stimulating the immunological response in ease: Ch. I. Propagation of the Agent in Cell Culture,” warm-blooded animals against a variety of tumors. 30 Proc. Soc. Exp. Biol. Med., 127:173-177 (1968). Avian BACKGROUND ART leukosis is presumably caused by a group B herpes vi The purified extraction residue fraction (antigen) of rus. See J. J. Solomon, R. L. Witter, K. Nazerian and B. microorganisms described in detail hereafter has been B. Burmester, “Studies on the Etiology of Marek's Dis found to be useful in the treatment of avian leukosis as 35 ease: Ch. II. Finding of Herpes Virus in Cell Culture,” well as in the treatment of warm-blooded animals, in Proc. Soc. Exp. Biol. Med., 127:177-182 (1968). A live cluding man, by stimulation of their immune response but attenuated form of turkey herpes virus vaccine has against a variety of tumors. The microorganism from been effective until recently in preventing the incidence which the purified extraction residue fraction is ob of Marek's disease tumors; however, its effectiveness tained is a pleomorphic, refractile and filterable form of 40 has markedly declined. The mechanism of protection is bacteria which has been repeatedly isolated from both not clear. Birds vaccinated develop a viremia with the human and animal malignant tissue and blood of tumor vaccine. See Kermani-ARAB, V. T. Moll, B. R. Cho, bearing hosts. These bacteria have been described by W. D. Davis and Y-S. Lu, “Effect of Cyclophospha many investigators, but not many agree on their taxon mide on the Response of Chickens to a Virulent Strain omy because of their pleomorphism. Using standard 45 of Marek's Disease Virus,' Infection & Immunity, culture and fermentation techniques, they resemble 12:1058–1064 (1975). A group of investigators have just common saphophytes. Their various growth phases recently reported protection of chickens against Rous have been described as viruses, depetheroids, micro sarcoma virus with the use of methanol extracts of BCG cocci, bacilli, and fungi. See V. W-C. Livingston and E. challenged with Rous sarcoma. They also reported that Alexander-Jackson, "A Specific Type of Organism 50 chickens with palpable tumors, and then treated, devel Cultivated from Malignancy: Bacteriology and Pro oped necrosis of the tumor. See Y. Markson, F. Dol posed Classification, Ann. N. Y. Acad. Sci, jansky and D. W. Weiss, “Effects of Prophylactic 174:636-654 (1970); G. J. Dominque and J.U. Schlegel, Treatment with the Methanol Extraction Residue Frac "Novel Bacterial Structures in Human Blood: Cultural tion of Tubercle Bacili (MER) on the Development of Isolation,” Infection & Immunity, 15:621-627 (1977); 55 Rous Sarcomas of Chickens Following Challenge with and V. W-C. Livingston and A. M. Livingston, "Some the Rous Sarcoma Virus,” Immunological Parameters of Cultural, Immunological and Biochemical Properties of Host-Tumor Relationships, Vol. 5, D. W. Weiss (Ed.), Progenitor Cryptocides,” Trans. N. Y. Acad. Sci, Academic Press, New York, pp. 51-59 (1978). 36:569-582 (1974). Treatment of warm-blooded animals by subcutaneous In 1970, Virginia Livingston-Wheeler and Eleanor 60 injection of the purified extraction residue fraction is Alexander-Jackson proposed a new taxon for this or based on stimulation of the immune response of the host ganism within the order Actinomycetale. They named to the injected substance. Immunotherapy is a therapeu the organism "Progenitor cryptocides.' Progenitor crypto tic approach to the treatment of cancer which is based cides has been repeatedly isolated from human and ani on the concept that there are distinctive antigens in or mal malignant tumors and has many interesting features, 65 on most tumor cells that distinguish them from normal such as pleomorphism, intermittent acid-fastness, a host cells. Most tumor immunologists favor the view unique ability to pass through bacterial filters, and the that potentially malignant cells constantly arise in the ability to produce chorionic gonadotropin. See V. W-C. body; but because of their foreign nature, they are nor 5,382,428 3 4 mally eliminated by the body's immune system. On chickens an effective immunostimulating amount of a occasion, however, tumor cells escape this immune purified extraction residue fraction of the microorgan surveillance and continue to reproduce, resulting in ism Progenitor cryptocides and an adjuvant.
Load more

Recommended publications
  • United States Patent (19) 11 4,410,510 Livingston-Wheeler Et Al
    United States Patent (19) 11 4,410,510 Livingston-Wheeler et al. 45) Oct. 18, 1983 (54) METHOD FOR PREPARING A PURIFIED EXTRACTION RESIDUE FRACTION AND OTHER PUBLICATIONS ITS USE IN STIMULATING THE IMMUNE Livingston et al., Trans. N.Y. Acad. Sci., vol. 36, p. 569 RESPONSE (1974). Cohen et al., Proc. Soc. Exp. Biol. and Med., vol. 152, 75) Inventors: Virginia Livingston-Wheeler, 84.92 pp. 408-410 (1976). Prestwick Dr., La Jolla, Calif. 92037; Maruo et al., Proc. Natl. Acad. Sci., vol. 76, pp. John J. Majnarich, Mercer Island, 6622-6626, 1979. Wash. Acevedo et al., Cancer, vol. 41, pp. 1217-1229, 1978. 73) Assignee: Virginia Livingston-Wheeler, San Acevedo et al., Infection and Immunity, vol. 24, pp. Diego, Calif. 920–924, 1979. Primary Examiner-Blondel Hazel (21) Appl. No.: 255,678 Attorney, Agent, or Firm-Seed and Berry 22 Filed: Apr. 20, 1981 57 ABSTRACT A method is disclosed for preparing a purified extrac Related U.S. Application Data tion residue fraction (antigen) of a microorganism iso lated from the blood or urine of a warm-blooded animal I63 Continuation-in-part of Ser. No. 128,919, Mar. 10, or tumor carried by a warm-blooded animal, the micro 1980, abandoned, which is a continuation of Ser. No. organism having the capacity to synthesize the polypep 957,206, Nov. 3, 1978, abandoned. tide hormone known as "chorionic gonadotropin' in its total form or in its alpha and beta subunits. The purified 51 Int. Cl.......................... A61K 39/02; C12N 1/20 extraction residue fraction is an activator and modula 52 U.S.
    [Show full text]
  • Alan Cantwell Papers
    http://oac.cdlib.org/findaid/ark:/13030/kt2s2033zz No online items Finding aid of the Alan Cantwell Papers Mor Fleisher-Leach ONE National Gay and Lesbian Archives 909 West Adams Boulevard Los Angeles, California 90007 Phone: (213) 741-0094 Fax: (213) 741-0220 Email: [email protected] URL: http://www.onearchives.org © 2009 ONE National Gay and Lesbian Archives. All rights reserved. Finding aid of the Alan Cantwell Coll2009-008 1 Papers Finding aid of the Alan Cantwell Papers Collection number: Coll2009-008 ONE National Gay and Lesbian Archives Los Angeles, California Processed by: Mor Fleisher-Leach Date Completed: August 31, 2009 Encoded by: Mor Fleisher-Leach © 2009 ONE National Gay and Lesbian Archives. All rights reserved. Descriptive Summary Title: Alan Cantwell papers Dates: 1957-2008 Collection number: Coll2009-008 Creator: Cantwell, Alan, 1934- Collection Size: 2 records boxes + 3 archive cartons + 1 archive half-carton (4.0 linear feet). Repository: ONE National Gay and Lesbian Archives. Los Angeles, California 90007 Abstract: Correspondence, articles, books, clippings, ephemera and audiovisual materials collected and created by Alan Cantwell during the course of his research relating to the theory that the AIDS epidemic is a man-made creation, its link to cancer bacteria, and the Hepatitis B experiments conducted on gay men from 1978-1981. The materials also discuss biological warfare, radiation, genocidal issues, and HIV. Included are a number of articles and books written by Cantwell as well as research material he used for his body of work. The authors of the research material include Robert Gallo, Virginia Livingston and Robert Strecker.
    [Show full text]
  • Breast Cancer and Cancer Bacteria
    Healing Cancer Naturally Breast Cancer and Cancer Bacteria Suppressed and forgotten cancer microbe research could hold the key to the cause and cure of this dread disease 2004 by Alan Cantwell, Jr., M.D. P.O.Box 29532, Los Angeles, CA 90029 Email: alancantwell at sbcglobal.net Despite a century of cancer research the cause of breast cancer remains unknown. Age, diet, stress, hormone factors, genetic predisposition, and cancer viruses are all suspected as possible causative factors, but totally ignored are infectious bacteria which have been implicated in breast cancer, as well as other forms of cancer. Over the past century physicians such as James Young, John Nuzum, Virginia Livingston, and numerous other researchers have reported on the existence of a specific microbe associated with cancer. The microbe is a ubiquitous, pleomorphic organism with a complex life cycle. A review of this literature suggests that the cancer microbe plays an important role in the development of malignancy. Because the etiology of cancer remains unknown, this research deserves further examination and reaappraisal. William Russell (1852-1940) and ”the parasite of cancer” A century ago when major diseases like tuberculosis, leprosy, and syphilis were discovered to be bacterial (not viral) infections, many physicians suspected bacteria might also cause cancer. At the close of the nineteenth century ( when the science of microbiology was in its infancy), many different microbes (variously called ”cancer coccidia,” ”sporozoons” and ”cancer parasites”) were cultured
    [Show full text]
  • Cancer and the Science of Denial with Breast Cancer/Long Island Breast
    Review Article J Tumor Med Prev Volume 1 Issue 3 - July 2017 Copyright © All rights are reserved by Lawrence Broxmeyer DOI: 10.19080/JTMP.2017.01.555563 Cancer and the Science of Denial –with Breast Cancer/Long Island Breast Cancer Dr. Lawrence Broxmeyer, MD* New York Institute of Medical Research, USA Submission: May 24, 2017; Published: July 14, 2017 *Corresponding author: Dr. Lawrence Broxmeyer, MD New York Institute of Medical Research, New York, USA, Tel: (718) 229-3694; Email: Abstract th The word ‘cancer’ is of Latin derivation and means crab. By the turn of the 20 Century organized medicine had come to the conclusion that it was not a matter of whether infectious disease caused cancer, but which one. Then, in 1910, certain American medical powers did a 180-degree rotation –abruptly deciding that cancer was not caused by a microbe. This flew in the face of over two hundred years of research in which a Actinomycetalescancer germ had been discovered and rediscovered. Of all the infectious possibilities for cancer, unquestionably the one class of microbes that has been long recognized to most consistently mimic and imitate ‘cancer’ at both clinical and tissue levels are the mycobacteria of the family of which tuberculosis and leprosy are premier examples. The association of TB with carcinoma was initially described about 200 years ago by Bayle who considered the lung malignancy ‘cavitation cancereuse’ to merely be one of the various types of tuberculosis. Ever since, almost as if in reflex to the obvious –the potential association between TB and subsequent development of cancer has drawn active investigation.
    [Show full text]
  • Further Evidence for Cancer As a Cell-Wall-Deficient Mycobacterial
    Research Article iMedPub Journals 2016 http://journals.imedpub.com Journal of MPE Molecular Pathological Epidemiology Vol. 1 No. 1: 5 Further Evidence for Cancer as a Cell-Wall- Lysenko AP1, Broxmeyer L2, Vlasenko VV3, Krasochko Deficient Mycobacterial Disease PA1, Lemish AP1 and Krasnikova EA1 Abstract 1 Institute of an Experimental Veterinary Science n. S.N. Wyshelesski, Belarus In 2014, Buehring reported that Bovine Leukemic Virus (BLV), a common oncogenic 2 N.Y. Institute of Medical Research in retrovirus of cattle, was present in some humans, primarily localized to the breast Bayside, New York, USA epithelium ― the very cell type from which most breast malignancies arise. By 3 Podolsk Center of Tuberculosis, Ukraine 2015, there appeared data (Buehring, 2015) supporting that as many as 37% of human breast cancer cases could be attributable to BLV exposure. But if recent estimates suggest over 83% of U.S. dairy operations are currently positive for Corresponding author: BLV, they also show that approximately 68% are positive for cell-wall-deficient Lawrence Broxmeyer, M.D Mycobacterium avium subspecies paratuberculosis (MAP). Although tubercular lung infection has been said to cause 11 times the incidence of lung cancer as normal control subjects, it is its cell-wall-deficient (CWD) forms (also called [email protected]. L-forms) that have recently repeatedly been found through genetic analysis and edu appropriate stains in such cancer tissue ― suggesting that CWD tuberculosis or atypical tuberculosis “is likely to be involved in the occurrence or development N.Y. Institute of Medical Research in Bayside, of lung carcinoma”. A similar relationship between tubercular L-forms and the New York, USA.
    [Show full text]
  • Royal Raymond Rife's Story Contains Crucial Information to Be Brought Into Focus Through Correlations with Both Old and New Knowledge
    Copyright © Barry Lynes 1987 All rights reserved. The use of any part of this publication reproduced, transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, or stored in a retrieval system, without the prior written consent of the publisher, is an infringement of the copyright Jaw. First printing March 1987 Second printing January 1989 Third printing August 1989 Fourth printing April 1992 Fifth printing August 1994 Sixth printing October 1997 Published in Canada by Marcus Books, P.O.Box 327, Queensville, Ontario, Canada LOG 1 RO. (905, 478-22trt-e, Fax (905) 478-8338 Cover designed by Doris Diehl ISBN 0-919951-30-9 CONTENTS The People Who Made This Book Foreword Author's warning Chapter 1:)The Cure forCancer Chapter 2: Bacteria and Virus Chapter 3: Medicine in America Chapter 4: The Man Who Found the Cure forCancer Chapter 5: The 1920s Chapter 6: The Early 1930s Chapter 7: "BX" -- The Virus of Cancer Chapter 8: Forward Motion: 1933-1934 Chapter 9: The Cancer Cure Works! Chapter IO: 1935: Climbing A Mountain Chapter 11: 1936: "Astounding" Clinic Results Chapter 12: 1937: Money Woes and Delays Chapter 13: 1938: Beam Ray Chapter 14: 1939: The Stonn Breaks Chapter 15: The Microscope and the Freq. Instrument Chapter 16: '46-'86: Rife's Theory Gains Acceptance Chapter 17: The Victims Chapter 18: Clarificationsand Explanations Chapter 19: How the Legal System Was Corruptedto Suppress Rife's Cancer Cure Selected Bibliography Appendices Beware of Exploiters! The People Who Made This Book- Royal R. Rife, born in 1888, was one of the greatest scien­ tific geniuses of the 20th century.
    [Show full text]
  • A History of Cancer Bacteria Research
    História da pesquisa da bactéria do câncer – L- formas , CWD Ron Falcone A HISTORY OF CANCER BACTERIA RESEARCH Overview Livingston's Findings The NCI Investigation The ACS Disputes a Cancer Bacterium Compelling New Evidence Toward A New Paradigm Conclusion Overview Since the 1920s, researchers have consistently isolated and cultured bacterial forms from cancer patients. Among the first investigators to have made the discovery was the Canadian physician Thomas Glover. Glover claimed that his organism displayed different forms including a filter-passing one closely resembling viruses. In what was perhaps the first long-term clinical trial involving a cancer therapy, two of Glover's colleagues---Dr.'s Clark and White---claimed favorable results for a variety of cancers using an anti-sera originally developed by Glover. The trial, however, was not controlled or randomized and the actual results would be difficult to determine by today's standards or protocols. The Glover anti-sera was made from horse antibodies derived from inoculation of human cancer bacteria (see how Glover's anti-sera was prepared). While Glover, White, Clark and Scott were among the first to document and then use as an anti-bacterial cancer vaccine, a New-Jersey physician named Virginia Livingston advanced Glover's controversial theories starting in the late 1940's. Livingston's Findings After conducting a number of experiments which included the darkfield and electron microscope, Livingston and several colleagues said they had corroborated Glover's bacteria theory via the established tenets of Robert Koch (i.e. "Koch's Postulates)[1]. Livingston named her cancer bacterium Progenitor cryptocides and she described it as a highly pleomorphic entity that behaved, in many ways, like Mycobacteria.
    [Show full text]
  • Cancer, the FDA, the AMA And
    CANCER, THE FDA, THE AMERICAN MEDICAL ASSOCIATION AND YOU Ricardo Beas – January 2011 "There are two basic theories as to what cancer is. The orthodox theory is that healthy body cells for some UNKNOWN reason mutate into cancer cells. The general idea is that the unknown reason is a result of aging, poor diet, heredity, a bad condition that slowly mutates into cancer, or something along those lines." The second theory – or the unaccepted theory – is that cancer is caused by a very unusual virus that changes from a virus to a bacterium and back to a virus again … This second theory is based on what is called pleomorphism. Research into this theory is not allowed … No money is available for such research and those who try to get funding hit a brick wall … The FDA, FTC and the AMA put every possible roadblock in their way.” From “Breakthrough: The Miracle Mineral Solution of the 21st Century,” by Jim Humble, 4th edition. By permission of the author. Here is a definition of cancer I found in a popular online dictionary site: Noun. Pathology. A malignant and invasive growth or tumor, esp. one originating in epithelium, tending to recur after excision and to metastasize to other sites. In the 1930’s Royal Raymond Rife, from San Diego, CA, invented a powerful non electronic microscope that could magnify up to 60,000x and had a resolution of 31,000x. Other non electronic microscopes only had a 2,500x power. The newer sophisticated microscopes are electronic. The difference between Rife’s Universal Microscope and our modern electron microscopes is that the Universal Microscope does not kill the specimens under observation and affords observation of natural living specimens in all circumstances, meaning it does not rely on fixing or staining to render visibility or definition, whereas the electron microscope does kill the specimen in observation.
    [Show full text]
  • The Russell Body the Forgotten Clue to the Bacterial Cause of Cancer © 2003, by Alan Cantwell, Jr
    Artigo histórico de 1890 escrito por Willian Russell talvez o primeiro a aventar a hipótese de um possível microorganismo como agente etiológico do câncer The Russell Body The Forgotten Clue To The Bacterial Cause Of Cancer © 2003, by Alan Cantwell, Jr. M.D. Nota: lembrar que Willian Russel escreveu artigo “An Adress on a Characteristic Organism of Cancer” no The Btritish Medical Journal Dec, 13, 1890 William Russell 1852-1940, as pictured in The British Medical Journal, August 24, 1940. William Russell and "the parasite of cancer" On December 3, 1890 William Russell, a pathologist in the School of Medicine at the Royal Infirmary in Edinburgh, gave an address to the Pathological Society of London in which he outlined his histopathologic findings of "a characteristic organism of cancer" that he observed microscopically in fuchsine-stained tissue sections from all forms of cancer that he examined, as well as in certain cases of tuberculosis, syphilis and skin infection. The parasite was seen within the tissue cells (intracellular) and outside the cells (extracellular). The size of Russell's parasite ranged from barely visible, up to "half again as large as a red blood corpuscle." The largest round forms were easily seen microscopically. The large size of some of these bodies suggested a fungal or yeast-like parasite. Russell provisionally classified the parasite as a possible "blastomycete" (a type of fungus); and called the forms "fuchsine bodies" because of their bluish-red staining qualities. Microbiology was still in its infancy in Russell's era, and it was generally thought that each microbe could only give rise to a single disease.
    [Show full text]
  • The Microbiology of Cancer and Its Potential Implications for Early Intervention Ron Falcone Website
    Microbiologia do câncer e implicações na intervenção precoce CWD , L-formas Hypothesis: The Microbiology of Cancer and its Potential Implications for Early Intervention Ron Falcone website Introduction The century's old debate as to whether bacteria are simply opportunistic infections "after the fact" or whether they can initiate cancer has been a contentious one. As early as the 1920's, Thomas Glover isolated a bacterium which he named "Glover's organism". Two decades later, Virginia Livingston believed that a single, ubiquitous pathogen she identified as a Mycobacterium (and which she named Progenitor cryptocides) was the primary cause of most human cancer. A handful of others also claimed discovery of a cancer-causing organism which appeared cameleon like, and seemed to defy one, universal taxonomy. From a mainstream standpoint, scientists disagreed with Glover and his later contemporaries, instead arguing that cancer-related pathogens were simply opportunists, contaminating diseased tissues after the fact. But in the 1990's, Shy Chung Lo of the Armed Services Institute of Pathology cultured Mycoplasma fermentans from cancer, injected the organism into animals, and was then able to induce cancer; in effect, Lo had established Koch's Postulates, proving that bacteria were indeed able to directly cause cancer [1]. Other investigators corroborated Lo's Mycoplasma research. For example, Chan reported the prevalence of mycoplasmal DNA in ovarian cancer[2]; Schmidhauser demonstrated that the p37 gene associated with mouse sarcoma originates from Mycoplasma and that a proportionate increase in malignant invasiveness was related to such exposure[3]; Ushio found that Mycoplasma-infected cells have a higher ability to metastasize in vivo than non-infected cells[4]; and Bogoch demonstrated that Mycoplasma secrete a similar polysaccharide used by cancer antigens to avoid immune-system recognition.
    [Show full text]
  • Inflammation-Induced Cancer: Crosstalk Between Tumours, Immune Cells and Microorganisms
    REVIEWS Inflammation-induced cancer: crosstalk between tumours, immune cells and microorganisms Eran Elinav1*, Roni Nowarski2*, Christoph A. Thaiss1*, Bo Hu2,3*, Chengcheng Jin2,4*and Richard A. Flavell2,5 Abstract | Inflammation is a fundamental innate immune response to perturbed tissue homeostasis. Chronic inflammatory processes affect all stages of tumour development as well as therapy. In this Review, we outline the principal cellular and molecular pathways that coordinate the tumour-promoting and tumour-antagonizing effects of inflammation and we discuss the crosstalk between cancer development and inflammatory processes. In addition, we discuss the recently suggested role of commensal microorganisms in inflammation- induced cancer and we propose that understanding this microbial influence will be crucial for targeted therapy in modern cancer treatment. Less than 10% of all cancers are caused by germline muta- infection6. Although the origin and molecular signals tions; the remainder are caused by acquired somatic mutations that drive inflammation during infection may be distinct and environmental factors. In many cases, tumour from inflammation that is caused by other factors, such as development is linked to chronic infection, dietary obesity, noxious inhalants and autoimmunity, many of the factors, obesity, inhaled pollutants, tobacco use or biological concepts that drive and regulate inflammation- autoimmunity1. The unifying principle that underlies associated cancer are similar across the various aetiolo- 1Department of Immunology, these processes is chronic inflammation, which is an gies. Even more intriguing is the emerging possibility Weizmann Institute of aberrantly prolonged form of a protective response to that variation in the commensal microbial ecosystems Science, 100 Herzl Street, a loss of tissue homeostasis2.
    [Show full text]
  • Is Cancer Just an Incurable Infectious Disease?
    Medical Hypotheses (2004) 63, 986–996 http://intl.elsevierhealth.com/journals/mehy Is cancer just an incurable infectious disease? L. Broxmeyer* 148-14A Eleventh Avenue, Whitestone, NY 11357, USA Received 1 April 2004; accepted 5 May 2004 Summary The word ‘cancer’ is of Latin derivation and means crab. By the turn of the 20th Century organized medicine had come to the conclusion that it was not a matter of whether infectious disease caused cancer, but which one. For over two hundred years a cancer germ had been discovered and rediscovered, named and renamed, each scientist adding to the knowledge, but to no avail. Then, in 1910, certain American medical powers did a 180-degree rotation, deciding that cancer was not caused by a microbe, and that anyone who thought otherwise was a heretic, a charlatan or a quack. But Dr. Virginia Livingston and her network were none of the above, their meticulous peer- reviewed research and publications, done at the height of US post World War II technology. And Dean Burk, Head of Cell Chemistry at the NCI went so far as to say that Livingston’s cancer germ was as real and certain as anything known about cancer. Researcher, MD Alan Cantwell Jr. grew up thinking that all germs responsible for the important diseases were supposed to have already been discovered. But much to his dismay, he found one that was left out: the cancer germ. Cantwell already knew that for finding this, Livingston had already been branded by traditional medicine, leaving what he thought to be perhaps the major discovery of the 20th century largely discredited.
    [Show full text]